• Journal of Korean Medicine Rehabilitation
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• v.31 no.2
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• pp.1-14
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• 2021

Objectives We evaluated the improving effects of Taeksa-tang (TST) using 3T3-L1 cells and C57BL/6 mice were fed on a high-fat diet. Methods The anti-radical activities of TST were studied using 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid). The content of total polyphenol was measured using Folin-Ciocalteu reagent, whereas aluminum chloride colorimetric method was used for the content of total flavonoid. Moreover, the factors related to lipid profile and the protein expressions such as 𝛽-oxidation and anti-oxidant enzyme were analyzed using serum and western blotting of 3T3-L1 cells. Additionally, we examined lipolysis through glycerol appearance in mouse adipose tissue. Results TST treatment showed strong free radical scavenging activities with half maximal inhibitory concentration and the presence of a amount of total polyphenol and total flavonoid. TST treatment significantly increased factors related to 𝛽-oxidation such as carnitine palmitoyl transferase-1 and uncoupling protein 2 via the phosphorlyation of liver kinase B1 (LKB1) and AMP-activated protein kinase (AMPK). Moreover, the protein expressions of anti-oxidant enzyme and lipolysis were significantly elevated by TST administration. In addition, TST supplementation lowered serum malondialdehyde, triglyceride, and total cholesterol levels compared with the control group. Taken together, these data suggest that TST treatment regulated lipid parameters via the increase of 𝛽-oxidation by LKB1-AMPK signaling pathway. Conclusions TST may have a potential remedy in the prevention and treatment of obesity. Therefore, this study may provide the scientific basis for TST use.

• Food Engineering Progress
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• v.15 no.2
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• pp.155-161
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• 2011

The aim of this study was to determine the effects of different extrusion conditions on the saccharification characteristics( initial reaction velocity, reaction rate constant, yield) of extruded corn starch. Extruded corn starch-water slurries were mixed with alpha-amylase for the enzymatic saccharification. The saccharification yield of extruded corn starch was high at lower feed moisture content and higher barrel temperature. The solubility of extrudates increased with increase in the SME input which increased with increase in the feed moisture content. Starch hydrolysates having DE 63.8 was obtained after 2 hr reaction. The initial reaction velocity of the extrudate slurry with alpha-amylase was higher with decrease in the feed moisture content. The initial reaction velocity of extruded corn starch was the highest ($2.26{\times}10^{-3}mmol/mL{\cdot}min$) at 25% feed moisture content and $120^{\circ}C$ barrel temperature, 250 rpm screw speed. The pregelatinized starch was $1.83{\times}10^{-3}mmol/mL{\cdot}min$ as a control. Reaction rate constant was a similar trend to initial reaction velocity.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.2
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• pp.404-411
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• 2019

In this study, the HEC-HMS was applied to determine rainfall-runoff processes for the Gokgyuchun basin. Several sub-basins have large-scale reservoirs for agricultural needs and they store large amounts of initial runoff. Three infiltration methods were implemented to reflect the effect of initial loss by reservoirs: 'SCS-CN'(Scheme I), 'SCS-CN' with simple surface method(Scheme II), and 'Initial and Constant rate'(Scheme III). Modeling processes include incorporating three different methods for loss due to infiltration, Clark's UH model for transformation, exponential recession model for baseflow, and Muskingum model for channel routing. The parameters were calibrated using an optimization technique with trial and error method. Performance measures, such as NSE, RAR, and PBIAS, were adopted to aid in the calibration processes. The model performance for those methods was evaluated at Gangcheong station, which is the outlet of study site. Good accuracy in predicting runoff volume and peak flow, and peak time was obtained using the Scheme II and III, considering the initial loss, whereas Scheme I showed low reliability for storms. Scheme III did not show good matches between observed and simulated values for storms with multi peaks. Conclusively, Scheme II provided better results for both single and multi-peak storms. The results of this study can provide a useful tool for decision makers to determine master plans for regional flood control management.

• Journal of Life Science
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• v.29 no.3
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• pp.311-317
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• 2019

To investigate the effects of light on growth in fish, olive flounder (Paralichthys olivaceus) were reared under four kinds of monochromatic light-emitting diodes (LEDs) at violet (400 nm), blue (465 nm), green (508 nm), and red (635 nm) wavelengths, along with a white fluorescent lamp as control. The rearing experiments were carried out with 15 fish per tank under different wavelength illumination at the same intensity. After rearing the fish under a 12 hr:12 hr light:dark photoperiod for 60 days, percentage increases in weight gain of $269.92{\pm}13.02$, $363.21{\pm}3.74$, $433.22{\pm}4.83$, $290.17{\pm}11.83$, and $340.74{\pm}26.58%$ and increases in specific growth rates (SGR) of $2.18{\pm}0.06$, $2.56{\pm}0.07$, $2.79{\pm}0.01$, $2.27{\pm}0.05$, and $2.47{\pm}0.10$ were observed in fish grown under the illumination of red, blue, green, and violet LEDs and the white fluorescent light, respectively. The results show faster growth in fish reared under green LEDs, but slower growth in those reared under red light. Differences in most blood parameters were minor, aside from an increased level of glutamic oxaloacetic transaminase in the fish grown under red LED illumination. Histological analysis of the retina showed few changes in the ratio of photoreceptor layer thickness to total retina thickness in fish reared under the green LEDs compared to those in other illumination groups. These results indicate that green LED light can foster increased growth in olive flounder with no distinct harmful effects on their light-sensitive photoreceptor layers.

• Journal of Microbiology and Biotechnology
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• v.29 no.7
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• pp.1083-1095
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• 2019

Butyrate is known to play a significant role in energy metabolism and regulating genomic activities that influence rumen nutrition utilization and function. Thus, this study investigated the effects of an isolated butyrate-producing bacteria, Clostridium saccharobutylicum, in rumen butyrate production, fermentation parameters and microbial population in Holstein-Friesian cow. An isolated butyrate-producing bacterium from the ruminal fluid of a Holstein-Friesian cow was identified and characterized as Clostridium saccharobutylicum RNAL841125 using 16S rRNA gene sequencing and phylogenetic analyses. The bacterium was evaluated on its effects as supplement on in vitro rumen fermentation and microbial population. Supplementation with $10^6CFU/ml$ Clostridium saccharobutylicum increased (p < 0.05) microbial crude protein, butyrate and total volatile fatty acids concentration but had no significant effect on $NH_3-N$ at 24 h incubation. Butyrate and total VFA concentrations were higher (p < 0.05) in supplementation with $10^6CFU/ml$ Clostridium saccharobutylicum compared with control, with no differences observed for total gas production, $NH_3-N$ and propionate concentration. However, as the inclusion rate (CFU/ml) of C. saccharobutylicum was increased, reduction of rumen fermentation values was observed. Furthermore, butyrate-producing bacteria and Fibrobacter succinogenes population in the rumen increased in response with supplementation of C. saccharobutylicum, while no differences in the population in total bacteria, protozoa and fungi were observed among treatments. Overall, our study suggests that supplementation with $10^6CFU/ml$ C. saccharobutylicum has the potential to improve ruminal fermentation through increased concentrations of butyrate and total volatile fatty acid, and enhanced population of butyrate-producing bacteria and cellulolytic bacteria F. succinogenes.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.12
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• pp.1930-1938
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• 2018

Objective: The present study attempted to determine safe and sufficient growth promoters in poultry feeding. Methods: A total of 520 seven-day-old quail chicks were randomly allotted to eight treatment groups in a $4{\times}2$ factorial design experiment to evaluate the effect of different levels of humic acid (HA) and black cumin (BC) seed and their interactions on growth, carcass traits, gut microbes, and blood chemistry of growing quails. Quails were randomly distributed into 8 groups in a $4{\times}2$ factorial design, included 4 HA levels (0, 0.75, 1.5, and 2.25 g/kg diet) and 2 BC levels (0 or 5 g/ kg diet). Results: Increasing HA level associated with a gradual increase in final weight, feed intake and body weight gain along with an improvement in feed conversion ratio. Dietary addition of 5 g BC powder/kg diet gave similar results. The highest level of HA (2.25 g/kg diet) recorded the best values of carcass weight, breast yield, intestinal length, and intestinal weight comparing with the control and other HA levels. Total viable microbial counts decreased (p<0.05) with increasing levels of HA except the intermediate level (1.5 g/kg diet). The concentration of serum cholesterol and low density lipoprotein (excluding that 0.75 g HA) decreased (p<0.05) and high density lipoprotein increased (p = 0.034) along with increasing HA level. The interaction between the $2.25g\;HA{\times}5g$ gave the best results regarding most studied parameters. Conclusion: These findings indicated that HA combined with BC could be used as effective growth promoters, with the recommended level being 2.25 g HA+5 g BC/kg of quail diet.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.24 no.3
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• pp.121-130
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• 2018

'Mats-kyeong-chae (Brassica lee ssp. namai cv. Tongssamchoo)', a new type of sweet Korean cabbage for fresh wrapping and soup cooking, was released by the Korea National College of Agriculture and Fisheries. The shelf-life and quality changes of this new cultivar was evaluated by packaging with PP (polypropylene) film bag and storing at three temperatures ($5^{\circ}C$, $15^{\circ}C$, and $25^{\circ}C$). As control, the cabbage was packaged in a corrugated paper box and stored at $25^{\circ}C$. At the storage temperature of $5^{\circ}C$, the degree of freshness, weight loss, hue angle and SPAD on cabbage packaged with PP film bag maintained its good quality and exhibited a longer period of marketable life compared to the samples stored in other treatments. Results showed that the use of PP film packaging and storing at $5^{\circ}C$ could be the optimum condition for commercial storage of 'Mats-kyeong-chae'. Further investigation on other quality parameters according to different packaging methods would be useful in maintaining the quality of 'Mats-kyeong-chae' at postharvest stages.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.6
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• pp.826-833
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• 2019

Objective: This study was performed to evaluate whether hot temperature and rumen-protected fat (RPF) supplementation affect growth performance, rumen characteristics, and serum metabolites in growing stage of Korean cattle steers. Methods: Twenty Korean cattle steers ($230.4{\pm}4.09kg$ of body weight [BW], $10.7{\pm}0.09months$ of age) were divided into a conventional control diet group (n = 10) and a 0.8% RPF supplementation group (n = 10). Steers were fed 1.5% BW of a concentrate diet and 4 kg of tall fescue hay for 16 weeks (July 10 to August 6 [P1], August 7 to September 3 [P2], September 4 to October 1 [P3], October 2 to 30 [P4], of 2015). Results: The mean temperature-humidity index (THI) was higher (p<0.001) in P1 (76.8), P2 (76.3), and P3 (75.9) than in P4 (50.9). The mean THI of P1-3 were within the alert heat stress (HS) category range according to previously reported categories for feedlot cattle, and the mean THI of P4 was under the thermo-neutral range. Neither month nor RPF supplementation affected (p>0.05) average daily gain and gain to feed ratio. Month and RPF supplementation affected concentrations of glucose, albumin, and high-density lipoprotein (HDL); those of albumin and glucose tended to decrease (p<0.10), but HDL concentration increased (p<0.01) by RPF supplementation. Neither month nor RPF affected (p>0.05) ruminal pH, $NH_3-N$, and volatile fatty acid concentrations, whereas the C2:C3 ratio was affected (p<0.05) by month. Conclusion: Korean cattle may not have been significantly affected by alert HS during the growing stage. Growth performance was higher during hotter months, although some changes in blood metabolites were observed. The RPF supplementation affected some blood lipids and carbohydrate metabolites but did not affect growth performance.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.1
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• pp.14-22
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• 2019

Objective: The aim of the study was to find a possible association between the ${\beta}-$ and ${\kappa}-casein$ and ${\beta}-lactoglobulin$ genotypes and important milk physiochemical and technological characteristics such as acidity, alcohol stability, the contents of some minerals and the parameters of acid fermentation ability (FEA) in Czech Fleckvieh Cattle. Methods: Milk and blood samples were collected from 338 primiparous Czech Fleckvieh cows at the same stage of lactation. The genotypes of individual cows for ${\kappa}-casein$ (alleles A, B, and E) and ${\beta}-lactoglobulin$ (alleles A and B) were ascertained by polymerase chain reaction-restriction fragment length polymorphism, while their ${\beta}-casein$ (alleles $A^1$, $A^2$, $A^3$, and B) genotype was determined using melting curve genotyping analysis. The data collected were i) milk traits including active acidity (pH), titratable acidity (TA), alcohol stability (AS); calcium (Ca), phosphorus (P), sodium (Na), magnesium (Mg), and potassium (K) contents; and ii) yoghurt traits including active acidity (Y-pH), titratable acidity (Y-TA), and the counts of both Lactobacilli and Streptococci in 1 mL of yoghurt. A linear model was assumed with fixed effects of herd, year, and season of calving, an effect of the age of the cow at first calving and effects of the casein and lactoglobulin genotypes of ${\beta}-CN$ (${\beta}-casein$, CSN2), ${\kappa}-CN$ (${\kappa}-casein$, CSN3), and ${\beta}-LG$ (${\beta}-lactoglobulin$, LGB), or the three-way interaction between those genes. Results: The genetic polymorphisms were related to the milk TA, AS, content of P and Ca, Y-pH and Lactobacilli number in the fresh yoghurt. The CSN3 genotype was significantly associated with milk AS (p<0.05). The effect of the composite CSN2-CSN3-LGB genotype on the investigated traits mostly reflected the effects of the individual genes. It significantly influenced TA (p<0.01), Y-pH (p<0.05) and the log of the Lactobacilli count (p<0.05). Conclusion: Our findings indicate that the yoghurt fermentation test together with milk proteins genotyping could contribute to milk quality control and highlight new perspectives in dairy cattle selection.

• Journal of the Korea institute for structural maintenance and inspection
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• v.23 no.1
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• pp.171-178
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• 2019

Fiber-reinforced polymer (FRP) composites have proved to be reliable as strengthening materials. Most of existing studies used single types of FRP composites. Therefore, in this experimental study, carbon FRP sheet, aramid FRP sheet, and hybrid FRP plate including glass fibers were fabricated, and the effect of pre-stressed FRP composites on flexural strengthening of reinforced concrete (RC) beams was investigated. In total, eight RC beam specimens were fabricated, including one control beam (specimen N) without FRP composites and seven FRP-strengthened beams. The main parameters were type of FRP composite, the number of anchors used for pre-stressing, and thickness of FRP plates. As a result, the beam strengthened with pre-stressed FRP plate showed superior performance to the non-strengthened one in terms of initial strength, strength and stiffness at yielding, and ultimate strength. As the number of anchors and thickness of FRP plate (i.e., amount of FRP plates) increased, the strengthening effect increased as well. When hybrid FRP plates were pre-stressed, the strengthening effect was higher in comparison with pre-stressed single type FRP plate.