• Title/Summary/Keyword: continuous light condition

Search Result 79, Processing Time 0.024 seconds

Phytochrome Signal Transduction Regulates Anthocyanin Biosynthesis in Cell Suspension Cultures of Vitis vinifera (포도 세포현탁배양계에서 Phytochrome 신호전달에 의해 조절되는 안토시아닌 생합성)

  • Choi, Kwan-Sam;Kim, Sun-Kyung;In, Jun-Gyo;Shin, Dong-Ho
    • Journal of Plant Biotechnology
    • /
    • v.31 no.3
    • /
    • pp.239-248
    • /
    • 2004
  • This experiment was carried out to confirm that phytochrome regulates anthocyanin bio-synthesis during cell suspension culture system of grape or not. In suspension culture of grape, maximum accumulation of anthocyanin was observed at the stationary phase under continuous white light condition. From mono-chromatic light interruption for 24h at the 4th or 7th day on the suspension cultured cells, the anthocyanin accumulation was highly enhanced at the light interruption at 7th day than 4th day under all monochromatic light treatment. However, the cell growth patterns were not affected by any light treatment. In the darkness, the anthocyanin synthesis was very low but remarkably increased by blue light or red light irradiation. However, the increase of anthocyanin accumulation by blue or red light was suppressed by far-red light in the suspension cells of grape. This suppression by far-red light on the anthocyanin synthesis also observed on the cells treated red or far-red light alternatively. These results implied that phytochrome regulation system may be involved in the anthocyanin biosynthesis of the suspension grape cells. By RNA expression analysis, chalcone synthase (CHS) gene was expressed highly by blue and red light but low by far-red light. The synergistic increase of CHS gene expression was also observed at the treatment of blue light followed by red for 24h. This result may explain the increase of anthocyanin accumulation in B/R treatment. Although the expression of phytochrome gene (PHYA or PHYB) was not highly increased by all light treatment (blue, red, and far-red light) the expression of both PHYA gene and PHYB gene was increased a little in cells treated red or far-red light. In grape suspension cells, the red light enhanced the anthocyanin synthesis, whereas the far-red light was suppressed. Although it was not confirmed whether or not phytochrome gene is activated in anthocyanin accumulating grape cells, we believed that anthocyanin biosynthesis in grape cells may be regulated under phytochrome signal transduction system.

Wastewater from Instant Noodle Factory as the Whole Nutrients Source for the Microalga Scenedesmus sp. Cultivation

  • Whangchenchom, Worawit;Chiemchaisri, Wilai;Tapaneeyaworawong, Paveena;Powtongsook, Sorawit
    • Environmental Engineering Research
    • /
    • v.19 no.3
    • /
    • pp.283-287
    • /
    • 2014
  • Cultivation of microalgae using wastewater exhibits several advantages such as nutrient removal and the production of high valuable products such as lipid and pigments. With this study, two types of wastewater from instant noodle factory; mixed liquor suspended solids (MLSS) and effluents after sedimentation tank were investigated for green microalga Scenedesmus sp. cultivation under laboratory condition. Optimal wastewater dilution percentage was evaluated in 24 wells microplate. MLSS and effluent without dilution showed the highest specific growth rate (${\mu}$) of $1.63{\pm}0.11day^{-1}$ and $1.57{\pm}0.16day^{-1}$, respectively, in which they were significantly (p < 0.05) higher than Scenedesmus sp. grown in BG11 medium ($1.08{\pm}0.14day^{-1}$). Ten days experiment was also conducted using 2000 ml Duran bottle as culture vessel under continuous light at approximately 5000 lux intensity and continuous aeration. It was found that maximum biomass density of microalgae cultivated in MLSS and effluent were $344.16{\pm}105.60mg/L$ and $512.89{\pm}86.93mg/L$ respectively and there was no significant (p < 0.05) difference on growth to control (BG11 medium). Moreover, cultivation microalgae in wastewater could reduce COD in wastewater by 39.89%-73.37%. Therefore, cultivation of Scenedesmus sp. in wastewater from instant noodle factory can yield microalgae biomass production and wastewater reclamation using photobioreactor simultaneously.

Degradation Efficiencies of Gas Phase Hydrocarbons for Photocatalysis Reactor With TiO2Thin Film (TiO2광촉매 반응기의 기체상 탄화수소의 분해효율)

  • 이진홍;박종숙;김진석;오상협;김동현
    • Journal of Korean Society for Atmospheric Environment
    • /
    • v.18 no.3
    • /
    • pp.223-230
    • /
    • 2002
  • Titania photocatalytic oxidation reactors were studied to investigate degradation efficiencies of hydrocarbons. In general, it is well known phenomena that thin layered titania oxidizes most of hydrocarbons to carbon dioxide and water under UV light. In this study, degradation efficiencies were measured due to changes in reactor structures, UV sources, the number of titania coatings, and various hydrocarbon chemicals. It was proven that gas degradation efficiencies are related to such factors as UV transmittance of coating substance, collision area of surface, and gas flow rate. For packing type annular reactor, about 98% degradation efficiency was achieved for achieved for propylene of 500 ppm level at a flow rate of 100 ml/min. Several gases were also tested for double-coated titania thin film under the condition of continuous flow of 100 ml/min and 365 nm UV source. It was shown that degradation efficiencies were decreasing in the order: $C_3$ $H_{6}$, n-C$_4$ $H_{10}$, $C_2$ $H_4$, $C_2$ $H_2$, $C_{6}$ $H_{6}$ and $C_2$ $H_{6}$./. 6/./.

광합성 미세조류인 Chlorococcum littorale을 이용한 이산화탄소의 생물학적 고정화

  • Kim, Tae-Ho;Sung, Ki-Dong;Lee, Jin-Suck;Lee, Joon-Yeop;Ohh, Sang-Jip;Lee, Hyeon-Yong
    • Microbiology and Biotechnology Letters
    • /
    • v.25 no.3
    • /
    • pp.235-239
    • /
    • 1997
  • Chlorococcum littorale has been grown in high $CO_2$ concentrations to utilize $CO_2$ gas in the polluted air. The effect of incident light intensity on the specific growth rate is expressed by a photoinhibition model, showing half- saturation constant, $K_0\;as\;8\;(W/m^2)$ and inhibition constant, Ki as 35 $(W/m^2)$. The maximum specific growth rate was also estimated as 0.095 (1/day) under this condition. This strain maintained the optimum growth rate in 20% of $CO_2$ gas but 50% of input $CO_2$ gas is the maximum concentration considering the economical efficiency. The maximum Specific $CO_2$ consumption rate, $qCO_2$ was measured as 17.48 (mg $CO_2/g$ dry wt./day) in batch cultivation, 11.2 (mg $CO_2/g$ dry wt./day) in fed-batch cultivation and 10.87 (mg $CO_2/g$ dry wt./day) at 0.065 (1/day) of dilution rate in continuous cultivation. The chemical composition of the biomass obtained from this process showed 32.5% of protein, 27.5% of lipid, 16.5% of carbohydrate and ash 11.7%.

  • PDF

An Analysis of Riparian Vegetation Distribution Based on Physical Soil Characteristics and Soil Moisture Content -Focused on the Relationship between Soil Characteristics and Vegetation- (토양의 물리적 특성 및 수분조건에 다른 하반식물의 분포 -토양환경과 식생과의 관계를 중심으로-)

  • 안홍규
    • Journal of the Korean Institute of Landscape Architecture
    • /
    • v.28 no.5
    • /
    • pp.39-47
    • /
    • 2000
  • This study is to investigate the conditions closely related to the establishment of vegetation in the riparian zone: the soil condition, an important factor along with climate and light. Especially, the soil structure of the microtopographical formations in the specific area known as the riparian microtopographical zone investigated. In addition, the effect of the riparian microtopographical features on the ground water level, soil moisture content, and vegetation was studied. The results of this study are as follows; 1) At all sample sites, below the sand layer, a gravel layer is always present. This is the result of past floods. 2) Although Salix koreensis experiences frequent disturbances such as increase in river level and floods, this vegetation establishes itself in the most secure are in the microtopographical zone. 3) The growth of Phragmites japonica is closely related to the underground water level. 4) It is clear that Miscanthus sacchariflorus grows concentrated in dry areas. 5) The soil accumulation conditions differ according to the soil moisture content of each microtopgraphical feature. Accordingly, the moisture content of the soil is clearly different within the microtopographical zone. The continuous and long-term investigation and research on the relation of riparian reproduction and the relevance with location surrounding factors are necessary in the future.

  • PDF

Formation of Oxidative Cleavage from Phytofluene by Autoxidation (자동산화에 의한 Phytofluene으로부터 산화개열산물의 생성)

  • 김선재
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.29 no.4
    • /
    • pp.568-574
    • /
    • 2000
  • Phytofluene was subjected to ozonolysis in ice-cold dichloromethane. The ozonolysis products were fractionated with a silica column and the carbonyl fraction was analyzed by ODS-HPLC with a photodiode array detector. Phytofluene was solubilized in 5% tween 40, and then oxidized by incubating under dim yellow light at 37$^{\circ}C$, 24 hr with continuous shaking. Carbonyl compound and acidic compound were produced. In comparison with autoxidation and ozonolysis, each compound showed the same retention time and UV-vis spectra were identical to the reference cleavage products prepared by ozeonolysis of phytofluene. Absorption spectrum of acidic compound was similar to that of standard 4,5-didehydrogeranyl geranyl acid which is known to possess biological activity. Thus, eccentric cleavage of phytofluene was confirmed to occur in vitro under oxidation condition.

  • PDF

A Study on Treatment of a Contaminated Soil by Oil using Continuous System of High Temperature Heating Element and Microwave (마이크로웨이브와 고온발열체를 이용한 연속식 공정의 유류오염토양 처리에 관한 연구)

  • Ha, Sang-An;You, Mi-Young
    • Journal of Soil and Groundwater Environment
    • /
    • v.17 no.1
    • /
    • pp.8-12
    • /
    • 2012
  • This study is maintains the condition of high temperature (above $600^{\circ}C$) within a short time using on microwave and high temperature heating elements. And removal characteristics according to changes in soil moisture, microwave power and temperature through the decomposition of the contaminated soil by oil. The difficulty resolvability material was sort of lubricating oil having long carbocyclic (C18-C50) and TPH removal rate reached 85.2% at 6 kW and $700^{\circ}C$ and thus the contaminant was removed 1,788 mg/kg within a process time of 40 minutes. In the case of light oil, gasoline contaminated soil, the removal amount showed 567 mg/kg and the treatment rate reached 98.4% at 6 kW, $500^{\circ}C$ and 20 minute. In the case of non-resolvability reached TPH concentrations on 2,000 mg/kg of worrisome level of soil contamination in the 3 zones at 6 kW, $700^{\circ}C$ and 30 minute. At the time, showed up processing costs 8,173 won per ton.

석탄 합성가스로부터 효율적인 생물학적 수소 생산에 관한 연구

  • 강환구;전희진
    • KSBB Journal
    • /
    • v.15 no.3
    • /
    • pp.268-273
    • /
    • 2000
  • A microbiological hydrogen production process was optimized. Anaerobic photosynthetic bacteria like Rhodospirillum rubrum which is known to produce hydrogen from carbon monoxide efficiently and remove sulfur was used. To evaluate the potenital of this microorganism the optimization of media fermentation condition light intensity and light requirement for CO conversionwas tried in batch cultures and the continuous fermenter was also applied for this process. The gas residence time on CO conversion was sought out to get high conversion of carbon monoxide to hydrogen. Through this study the possibility of microbial synthtics gas concersion process was proposed.

  • PDF

Q-Switched Nd YAG's SHG conversion techniques for a skin diseased treatment (피부질환 치료를 위한 Q-Switched Nd:YAG의 SHG 변환기술)

  • Kim, Whi-Young
    • Journal of the Korea Institute of Information and Communication Engineering
    • /
    • v.13 no.6
    • /
    • pp.1141-1149
    • /
    • 2009
  • Pulse style Nd: YAG Laser is suitable in skin remaking treatment, in compliance with the ramp continuous oscillation until of course normal takeoff, the Q-switch and mode motive takeoff the takeoff form which is various is possible and it is coming to be widely used in microsurgery and skin remaking promotion. According to therapeutic objective very it is important to control a energy density. Control of energy density the method which controls the pulse repetition rate of Laser output is mainly used. From the research which it sees pulse style Nd: It will be able to control the pulse repetition rate of YAG, the 2nd harmonic occurrence Laser (second harmonic generation: SHG) with the energy part of the light-wave which is a footnote wave number will hold and nonlinear decision it propagates and is converted by energy of the light-wave which is a footnote wave number the actual condition which and it applies the second harmonic occurrence in compliance with a secondary nonlinearity it leads and until skin deep part therapeutic possibility is the thing it will be able to observe simply.

Development of harmful ultraviolet blocking transparent flexible device using TiO2-x thin film process (TiO2-x 산화물 박막공정을 이용한 유해자외선차단 투명유연소재개발)

  • Kim, Geug Tae
    • Journal of the Korean Crystal Growth and Crystal Technology
    • /
    • v.29 no.3
    • /
    • pp.123-131
    • /
    • 2019
  • In this study, the development of transparent UV blocking material using $TiO_{2-x}$ oxide thin film process was developed. A process technology is related to a process technology for making a sample with ultraviolet-shielding property of visible light transmittance of 78 % or more (total light transmittance at 550 nm) and of a UV cut-off characteristic of more than 95 % at 315 nm in ultraviolet wavelength band. In this study, it is possible to establish a flexible device process condition of high performance ultraviolet (UV) shielding thin film, to design mixed type of transparent flexible device with heterogeneous characteristics and to formulate composite deposition technology, according to various market demands. Establishment of actual roll-to-roll continuous process and equipment and process technology will affect related industries greatly.