• Journal of Microbiology and Biotechnology
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• 제29권9호
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• pp.1453-1459
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• 2019

Zeaxanthin is an important pigment in the photo-protection mechanism of microalgae. However, zeaxanthin epoxidase, an enzyme involved in the accumulation and conversion of zeaxanthin, has not been extensively studied in microalgae. In this work, we report the expression pattern of zeaxanthin epoxidase in Dunaliella tertiolecta (DtZEP) at different light and diverse salinity conditions. To confirm the responsiveness to light conditions, the ZEP expression pattern was investigated in photoperiodic (16 h of light and 8 h of dark) and continuous (24 h of light and 0 h of dark) light conditions. mRNA expression levels in photoperiodic conditions fluctuated along with the light/dark cycle, whereas those in continuous light remained unchanged. In varying salinity conditions, the highest mRNA and protein levels were detected in cells cultured in 1.5 M NaCl, and ZEP expression levels in cells shifted from 0.6 M NaCl to 1.5 M NaCl increased gradually. These results show that mRNA expression of DtZEP responds rapidly to the light/dark cycle or increased salinity, whereas changes in protein synthesis do not occur within a short period. Taken together, we show that DtZEP gene expression responds rapidly to light irradiation and hyperosmotic stress. In addition, ZEP expression patterns in light or salinity conditions are similar to those of higher plants, even though the habitat of D. tertiolecta is different.

• 한국농림기상학회지
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• 제5권3호
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• pp.208-212
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• 2003

가로등과 보안등의 증가로 인한 야간조명의 확대와 야간 이동차량의 증가로 전조등 등 농경지 주변에 간헐적으로 조명이 이루어지고 있어 2000년부터 2001년까지 2년 동안 암기간과 야간조파가 벼와 콩의 출수 및 개화에 미치는 영향을 구명하고자 수행한 시험결과를 요약하면 다음과 같다. 12시간 암기 중 3시간마다 10분간 백열등(50∼60 lux)으로 조명한 야간조파 처리에 의하여 벼 생육은 두 품종 모두 암기처리 12시간에 비해 간장은 증가되었으며, 수당립수는 감소되었다. 벼의 출수는 야간조파시 암기처리 12시간에 비하여 일품벼 9일, 화성벼는 26일 지연되었으며, 12시간의 암기처리에 비하여 9시간 암기처리시 일품벼 47일, 화성벼는 41일 지연되었다. 야간조파에 의한 황금콩의 생육은 야간조파시 암기처리 12시간에 비해 경장파 절수가 증가되었으며, 협수는 감소되었고, 12시간의 암기처리에 비하여 9시간 암기처리시 경장 및 절수는 증가되었지만 협수는 감소되었다. 콩의 개화까지 소요일수는 야간조파시 암기처리 12시간에 비하여 4일 지연되었으며, 12시간의 암기처리에 비해 9시간 암기처리시 19일 지연되었다.

• 상하수도학회지
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• 제29권3호
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• pp.319-323
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• 2015

This study investigated the effects of the light conditions on the productivity of scenedesmus dimorphus in the continuous mass cultivation system. To compare the algal productivity according to the light conditions, S. dimorphus was cultivated continuously under the wide range of light intensity(200-600 PPFD) and various light wavelength(white light and red-blue mixed light). After 100 days of cultivation under the different light intensity, the productivity of S. dimorphus increased as light intensity decreased. So, the productivity was maximized as 100 mg/L/d when light intensity was 200 PPFD. In case of light wavelength, the productivity of S. dimorphus was enhanced about 20% with the white light compared to that of the red-blue mixed light. Consequently, the optimal light conditions for the continuous mass cultivation of S. dimorphus were 200 PPFD as light intensity and white light as light wavelength.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2006년도 춘계학술대회
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• pp.478-481
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• 2006

Energy is vital to global prosperity, yet dependence on fossil fuels as our primary energy source contributes to global climate change environmental degradation, and health problems. Hydrogen $(H_2)$ offers tremendous potential as a clean renewable energy currency. Hydrogen has the highest gravimetric energy density of any known fuel and is compatible with electrochemical and combustion processes for energy conversion without producing carbon-based emission that contribute to environmental pollution and climate change. Numerous methodologies have been developed for effective hydrogen production. Among them, the biological hydrogen production has gained attention, because hydrogen can be produced by cellular metabolismunder the presence of water and sunlight. The green alga Chlamydomonas reinhardtii is capable of sustained $H_2$ photoproduction when grown under sulfur deprived condition. Under sulfur deprived conditions, PSII and photosynthetic $O_2$ evolution are inactivated, resulting in shift from aerobic to anaerobic condition in the culture. After anaerobiosis, sulfur deprived algal cells induce a reversible hydrogenase and start to evolve $H_2$ gas in the light. According to above principle, we investigated the effect of induction parameters such as cell age, cell density. light intensity, and sulfate concentration under sulfur deprived condition We also developed continuous hydrogen production system by sulfate re-addition under sulfur deprived condition.

• 전자공학회논문지
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• 제50권8호
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• pp.151-161
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• 2013

본 논문에서는 입력전압을 감지하지 않는 전류연속/임계동작모드 active power factor correction(PFC) circuit을 제안하였다. 기존의 입력전압을 감지하지 않는 PFC circuit의 경우 출력전류가 낮은 경 부하 조건에서 DCM 동작을 수행하고, 이에 따라 PF가 감소하는 문제가 발생한다. 제안한 PFC circuit은 70KHz의 주파수로 CCM 동작을 수행하고, 경 부하 조건에서 최대 200KHz까지 스위칭 주파수가 가변되는 CRM 동작을 수행하도록 하였다. 이를 통해 경 부하 조건에서 PF가 감소하는 문제를 해결하였다. PFC controller IC는 $0.35{\mu}m$ BCDMOS 공정을 이용하여 제작하였으며, 240W급 PFC prototype을 제작하여 실험하였다. 제안한 PFC circuit은 기존의 PFC circcuit 대비 최대 10%의 역률이 향상되었고, IEC 61000-3-2 Class D 규격에 따른 경 부하 조건에서는 최대 4% 역률이 향상되었다.

• Current Photovoltaic Research
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• 제6권3호
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• pp.69-73
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• 2018

PV system is consisted with PV module, inverter and BOS(balance of system). To have robustic operation more than 20 years, the expected and guaranteed durability and reliability of products should be met. Almost components of PV system are qualified through IEC standards at test laboratory. But the qualification certificate of product does not ensure long-term nondefective operation. PV module's expected life time is nowadays more than 20 years and annual maximum power degradation ratio would be less than -1%. But the power degradation ratio is basically based on real data more than several years' record. Developing test method for ensuring annual maximum power degradation ratio is very need because there are many new products every month with new materials. In this paper, we have suggested new test method under continuous artificial light irradiation test condition for analyze expected maximum power drop ratio.

• Natural Product Sciences
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• 제2권2호
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• pp.90-95
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• 1996

When reserpine-producing cell strains of Rauwolfia serpentina were transferred from the dark to the light irradiation, the production of reserpine was extremely enhanced whereas the cell growth was suppressed. In an incubation period of 20 days, the most effective culture condition for reserpine production was the combination of 8 days of dark culture and following 12 days of light culture. The time courses of both cell growth and reserpine production were measured in vitro in order to clarify the effect of wave length range of light on the biosynthesis of reserpine. Although the growth of cultured cells which had been incubated under continuous red, yellow, and green lights, respectively, was similar to that of the cultured cells subcultured in the dark. The cells cultured under red light irradiation produced less reserpine than dark-grown cultures. Both blue and near-ultraviolet light inhibited the growth of cultured cells. The production of reserpine was strikingly enhanced by blue light, but was strongly inhibited by near-ultraviolet light.

• 한국자동차공학회논문집
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• 제25권1호
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• pp.111-115
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• 2017

This forged piston is proposed with a lighter weight and higher durability than a gravity casting piston for gasoline engines. However, a forged piston is very difficult to develop and mass-produce due to lack of basic technologies such as design, material and forging technique. First, we benchmarked existing forged pistons according to database design parameters. Second, we evaluated two solidification processes, continuous casting and spray forming, to produce heat-resistant alloy billets for forging. The spray forming process gives better mechanical properties at all temperatures, particularly at elevated temperatures except when poor formability is present. We used DEFORM simulation to determine the optimum process condition with billet from spray forming and successfully commercialized it with LF Sonata HEV.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2001년도 춘계학술대회 논문집
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• pp.655-659
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• 2001

Resident time of the continuous and Instantaneous centrifugal separator using that separates the light and heavy liquids by use of density difference is the orptical factor that affects significantly the chemical metial extraction and the productivity in the chemical and mechanical process. In this paper, the overflow of the device is investigated under consideration of the relationships between inclination angle of liquid feeding screw and the centrifugal force. From the design of the length of a centrifugal separator, the radiuses of rotor and housing, theoretical formulation on the contact radius of separation weir is established through the experiments. From the experiments, it is identified that how much the capacity of inlet impeller and the emulsion phenomenon depend on the screw angle of inlet impeller. Also, we investigate the separation condition and the resident times that are functions of the phase ratio and density.

• 산업기술연구
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• 제15권
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• pp.5-13
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• 1995

Natural gas, which is getting more important as a fuel, should be liquefied and shipped in a special tank. During transportation, a spill of liquefied natural gas(LNG) could occur by a collision or even an accident. As a result, violent explosion called the superheat-limit explosion(SLE) can take place in some cases, unexpectedly. Such explosion may result from the formation of a superheated liquid which has attained the superheat-limit temperature when hot(water) and cold(LNG) liquids come into contact. Natural gas mixtures can be considered as discrete light components plus continuous heavy fractions where several continuous distribution function can be adopted. This work is aiming at prediction of the superheat-limit explosion condition by suing continuous thermodynamics development of algorithm to predict.