• 제목/요약/키워드: continuous culture

검색결과 986건 처리시간 0.028초

The Impact of Shared Leadership on Organizational Commitment and Career Development in Startups

  • Chanuk Park;Sin-Bok Lee
    • International Journal of Advanced Culture Technology
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    • 제12권1호
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    • pp.60-74
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    • 2024
  • Due to the relative lack of concerns and research on organizational career development in startups, we study aimed to reveal the impact of shared leadership in startups on organizational career development through organizational commitment. The research method was an online survey targeting 200 startup members from April 11 to April 28, 2023, and a total of 195 copies were used in the final analysis. As a result of the study, first, among the shared leadership of startups, development and mentoring were found to have a positive impact on emotional commitment. Second, among the shared leadership of startups, planning and organizing, support and empathy were found to have a positive impact on continuous commitment. Third, planning and organizing, support and empathy had a positive impact on normative commitment. Third, among startups' shared leadership, planning and organizing, support and empthy had a positive impact on normative commitment. Fourth, emotional and continuous commitment were found to have a positive impact on improving career goals in organizational career growth. Fifth, emotional and continuous and normative commitment were each found to have a positive influence on the development of professional abilities in organizational career development. Lastly, emotional, continuous and normative commitment were all found to have a positive influence on the speed of promotion in organizational career growth. As a result, it is expected that the results of this study will be able to suggest shared leadership policy directions and goals for startups.

Insertional mutations exhibiting high cell-culture density HCD phenotypes are enriched through continuous subcultures in Chlamydomonas reinhardtii

  • Thung, Leena;He, Jing;Zhu, Qingling;Xu, Zhenyu;Liu, Jianhua;Chow, Yvonne
    • ALGAE
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    • 제33권1호
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    • pp.127-141
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    • 2018
  • Low efficiency in microalgal biomass production was largely attributed to the low density of algal cell cultures. Though mutations that reduced the level of chlorophyll or pigment content increased efficiency of photon usage and thus the cell-culture density under high-illumination growth conditions (e.g., >$500{\mu}mol\;photon\;m^{-2}\;s^{-1}$), it was unclear whether algae could increase cell-culture density under low-illumination conditions (e.g., ${\sim}50{\mu}mol\;photon\;m^{-2}\;s^{-1}$). To address this question, we performed forward genetic screening in Chlamydomonas reinhardtii. A pool of >1,000 insertional mutants was constructed and subjected to continuous subcultures in shaking flasks under low-illumination conditions. Complexity of restriction fragment length polymorphism (RFLP) pattern in cultures indicated the degree of heterogeneity of mutant populations. We showed that the levels of RFLP complexity decreased when cycles of subculture increased, suggesting that cultures were gradually populated by high cell-culture density (HCD) strains. Analysis of the 3 isolated HCD mutants after 30 cycles of subcultures confirmed that their maximal biomass production was 50-100% higher than that of wild type under low-illumination. Furthermore, levels of chlorophyll content in HCD mutant strains were similar to that of wild type. Inverse polymerase chain reaction analysis identified the locus of insertion in two of three HCD strains. Molecular and transcriptomic analyses suggested that two HCD mutants were a result of the gain-of-function phenotype, both linking to the abnormality of mitochondrial functions. Taken together, our results demonstrate that HCD strains can be obtained through continuous subcultures under low illumination conditions.

Kasugamycin 생산을 위한 연속 청정생물공정 개발 (Development of Continuous Clean Bioprocess for Kasugamycin Production)

  • 김창준;박선옥;장용근;전계택;이종대;김상용
    • 청정기술
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    • 제4권1호
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    • pp.45-59
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    • 1998
  • 방선균인 Streptomyces kasugaensis에 의해서 생산되는 이차대사산물인 kasugamycin 생산성 증대 및 단위 생산량 당 오염물질 배출량 저감을 위한 연속 고정화 배양을 수행하였다. 세포 고정화에 적합한 포자 수확을 위한 포자형성 촉진 배지 개발은 물론 고정화 담체로 사용한 셀라이트에 수확된 포자를 고정화시키는 방법을 확립하였다. 연속 고정화 배양 시 고정화세포의 유출을 방지함으로써 안정된 연속배양을 가능토록 하기위한 decantor 형태의 고정화세포 분리기가 장착된 반응기를 사용하였다. 희석 속도 및 공급액 중의 기질 농도(당 농도, 인 농도)를 변화시키며 생합성된 kasugamycin 생산성 및 화학적산소요구량(COD)을 측정하였으며 이들을 현탁세포를 이용한 회분식 발효에서의 kasugamycin 생산성 및 발효 폐액 중의 COD 값과 비교하였다. 고정화 세포를 이용한 연속배양에서의 kasugamycin 생산성이 현탁세포를 이용한 회분식 발효에 비해 2.5배 높았으며, 동시에 단위 생산량 당 COD가 2.3배 저감되는 것으로 나타났다. 이것으로부터 연속 고정화 배양이 현탁 회분식 배양에 비해 kasugamycin 생산성 및 오염물질 배출 측면에서 유리한 청정 공정임을 확인할 수 있었다.

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Gellan형 미생물 다당류의 연속생산 (Gellan-type Microbial Polysaccharide Production in Continuous Fermentation)

  • 정봉우;이은미장광엽김춘영
    • KSBB Journal
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    • 제9권1호
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    • pp.85-90
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    • 1994
  • Gellan형 미생물 다당류를 생산하는데 관련된 기초 자료를 얻기 위하여 배지조성, 배양온도 및 초기 pH등에 대한 플라스크 실험을 수행하였으며 이를 근거로 회분식, 유가식 및 연속배양을 실시하였다. 또한 배양액의 비뉴우튼성 유체 특성을 조사하였고 이때의 교반동력을 측정하였다. 플라스크 배양을 통하여 얻은 결과를 이용하여 회분식 배양의 최적조건을 확립하였으며 각종 metabolic parameter들을 추정하였다. 유가식과 연속식 배양을 통하여 생산성 향상을 시도한 결과 유가식과 연속식 배양에서 생산성 향상을 꾀할 수 있었다. 고점성 배양액에서의 물질전달 장해현상을 극복하기 위한 일련의 연구가 필요하다고 사료되며 이로부터 생산성 향상을 크게 기대할 수 있을 것으로 보인다.

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Zoogloea ramigera의 회분식, 유가배양, 연속배양에 의한 생물고분자 생산 (Biopolyrner Production of Zoogloea ramigera in Batch, Fed-Batch and Continuous Culture Processes)

  • 안대희;정윤철
    • 한국미생물·생명공학회지
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    • 제20권2호
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    • pp.196-202
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    • 1992
  • Zooloea ramigera 115를 사용하여 생물응집제로서 사용되는 생물고분자생산 실험을 하였다. 생물고분자 생산을 높이기 위하여 회분식, 유가배양, 연속배양방법을 사용하였다. 탄소원으로는 포도당, 유당, 당밀, 유청을 사용하였다. 기질이 포도당의 경우에는 C/N배 98일 때 생물고분자 생산 효과가 좋았으며, 유당의 경우에는 C/N비 30, 당밀과 유청의 경우에는 C/N비 60일 때 생물고분자 생산이 가장 좋았다. 유가배양 방법이 회분식 배양방법 보다 최종 생물고분자 생산이 우수하였다. C/N비를 달리한 2단계 연속 배양방법으로 생산성을 향상시켰다. 당밀의 경우 0.048$hr^{-1}$의 희석속도에서, 유청의 경우 0.096$hr^{-1}$에서 생산성이 가장 좋았다.

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리더십과 애자일 문화간의 관계에 관한 연구: 조직지원인식의 매개 효과를 중심으로 (A study on the Relationship between Leadership and Agile Culture: focusing on the mediating effect of Perceived Organizational Support(POS))

  • 김태완;유연우;홍정완
    • 융합정보논문지
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    • 제11권6호
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    • pp.226-242
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    • 2021
  • 최근 기업 경영환경의 변화에 대응하기 적합한 조직문화로 주목받고 있는 애자일 문화의 특성을 검토하고 리더십, 애자일 문화, 조직지원인식(POS)의 역할과 상호 관계를 밝히고자 하였다. 연구는 농협중앙회 및 산하의 자회사 직원들을 대상으로 진행하였고, 연구모형 검정에는 주로 SmartPLS 3.3.2를 사용하였다. 연구결과, 서번트리더십과 진성리더십은 모두 POS에 긍정적인 영향을 미쳤고, POS는 애자일 문화의 모든 하위차원(권한위임, 지속적인 학습, 집단지성)에 유의미한 영향을 미쳤다. 또한 POS는 위의 두 가지 리더십이 집단지성과 권한위임에 미치는 영향을 매개하였으나, 지속적 학습과의 관계에서는 매개 효과가 없었다. 결론적으로 본 연구는 애자일 문화를 구축하기 위해서는 애자일 문화에 적합한 리더십과 조직지원에 대한 직원들의 확신이 매우 중요하다는 것을 시사하고 있다.

메타놀자화균의 연속배양에 의한 균체생산의 온-라인 적응최적화 (Adaptive On-line Optimization of Cellular Productivity of Continuous Methylotroph Culture)

  • 이형춘;박정오
    • 한국식품영양학회지
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    • 제1권2호
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    • pp.31-36
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    • 1988
  • An adaptive on-line optimization method has been applied to test the ability to maximize the cellular productivity of a continuous methylotroph culture system which was simulated by a variable yield Monod-type model. Optimum dilution rate and productivity were successively obtained and maintained at all times by the algorithm that utilizes steepest descent technique as optimization method and recursive least-square method with forgetting factor as dynamic model identification.

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Production of Lactococcal Bacteriocin using Repeated-Batch and Continuous Cultures

  • Yoo, Jin-Young
    • Journal of Microbiology and Biotechnology
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    • 제2권4호
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    • pp.284-287
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    • 1992
  • Repeated-batch and continuous cultures of Lactococcus sp. 1112-1 were carried out for bacteriocin production using a glucose-casein medium. Repeated-batch culture did not efficiently enhanced the bacteriocin production. Continuous production was possible at the dilution rate of 0.4 $h^{-1}$. Maximum specific production rate ($Q^p$), bacteriocin production and biomass at the dilution rate were 347, 136 IU/g/h, 2, 121 IU/ml and 2.45 g/L, respectively.

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Evaluation of L-Lactic Acid Production in Batch, Fed-batch, and Continuous Cultures of Rhizopus sp. MK-96-1196 Using an Airlift Bioreactor

  • Liu, Tiejun;Miura, Shigenobu;Arimura, Tomohiro;Tei, Min-Yi;Park, Enoch Y.;Okabe, Mitsuyasu
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제10권6호
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    • pp.522-527
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    • 2005
  • Various processes which produce L-lactic acid using ammonia-tolerant mutant strain, Rhizopus sp. MK-96-1196, in a 3L airlift bioreactor were evaluated. When the fed-batch culture was carried out by keeping the glucose concentration at 30g/L, more than 140 g/L of L-lactic acid was produced with a product yield of 83%. In the case of the batch culture with 200g/L of initial glucose concentration, 121g/L of L-lactic acid was obtained but the low product yield based on the amount of glucose consumed. In the case of a continuous culture, 1.5g/L/h of the volumetric productivity with a product yield of 71% was achieved at dilution rate of $0.024\;h^{-1}$. Basis on these results three processes were evaluated by simple variable cost estimation including carbon source, steam, and waste treatment costs. The total variable costs of the fed-batch and continuous cultures were 88% and 140%, respectively, compared to that of batch culture. The fed-batch culture with high L-lactic acid concentration and high product yield decreased variable costs, and was the best-suited for the industrial production of L-lactic acid.