Insertional mutations exhibiting high cell-culture density HCD phenotypes are enriched through continuous subcultures in Chlamydomonas reinhardtii |
Thung, Leena
(Institute of Chemical Engineering and Sciences, A-STAR)
He, Jing (Ocean Research Centre of Zhoushan, Zhejiang University) Zhu, Qingling (Ocean College, Zhejiang University) Xu, Zhenyu (Ocean College, Zhejiang University) Liu, Jianhua (Ocean Research Centre of Zhoushan, Zhejiang University) Chow, Yvonne (Institute of Chemical Engineering and Sciences, A-STAR) |
1 | Polle, J. E. W., Kanakagiri, S. D. & Melis, A. 2003. tla1, a DNA insertional transformant of the green alga Chlamydomonas reinhardtii with a truncated light-harvesting chlorophyll antenna size. Planta 217:49-59. |
2 |
Pollock, S. V., Colombo, S. L., Prout, D. L. Jr., Godfrey, A. C. & Moroney, J. V. 2003. Rubisco activase is required for optimal photosynthesis in the green alga Chlamydomonas reinhardtii in a |
3 | Pollock, S. V., Pootakham, W., Shibagaki, N., Moseley, J. L. & Grossman, A. R. 2005. Insights into the acclimation of Chlamydomonas reinhardtii to sulfur deprivation. Photosynth. Res. 86:475-489. DOI |
4 | Sartory, D. P. & Grobbelaar, J. U. 1984. Extraction of chlorophyll a from freshwater phytoplankton for spectrophotometric analysis. Hydrobiologia 114:177-187. DOI |
5 | Shimogawara, K., Fujiwara, S., Grossman, A. & Usuda, H. 1998. High-efficiency transformation of Chlamydomonas reinhardtii by electroporation. Genetics 148:1821-1828. |
6 | Sizova, I., Fuhrmann, M. & Hegemann, P. 2001. A Streptomyces rimosus aphVIII gene coding for a new type phosphotransferase provides stable antibiotic resistance to Chlamydomonas reinhardtii. Genetics 277:221-229. |
7 | Sueoka, N. 1960. Mitotic replication of deoxyribonucleic acid in Chlamydomonas reinhardi. Proc. Natl. Acad. Sci. U. S. A. 46:83-91. DOI |
8 | Tam, L. W. & Lefebvre, P. A. 1993. Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis. Genetics 135:375-384. |
9 | Trapnell, C., Pachter, L. & Salzberg, S. L. 2009. TopHat: discovering splice junctions with RNA-Seq. Bioinformatics 25:1105-1111. DOI |
10 | Wang, Z. T., Ullrich, N., Joo, S., Waffenschmidt, S. & Goodenough, U. 2009. Algal lipid bodies: stress induction,purification, and biochemical characterization in wildtype and starchless Chlamydomonas reinhardtii. Eukaryot.Cell 8:1856-1868. DOI |
11 | Work, V. H., Radakovits, R., Jinkerson, R. E., Meuser, J. E., Elliott, L. G., Vinyard, D. J., Laurens, L. M. L., Dismukes, G. C. & Posewitz, M. C. 2010. Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complemented strains. Eukaryot. Cell 9:1251-1261. DOI |
12 |
Yoshioka, S., Taniguchi, F., Miura, K., Inoue, T., Yamano, T. & Fukuzawa, H. 2004. The novel Myb transcription factor LCR1 regulates the |
13 | Harris, E. H. 2001. Chlamydomonas as a model organism. Annu. Rev. Plant Physiol. Plant Mol. Biol. 52:363-406. DOI |
14 | Kang, S. G., Ortega, J., Singh, S. K., Wang, N., Huang, N. N., Steven, A. C. & Maurizi, M. R. 2002. Functional proteolytic complexes of the human mitochondrial ATPdependent protease, hClpXP. J. Biol. Chem. 277:21095-21102. DOI |
15 | Leon-Banares, R., Gonzalez-Ballester, D., Galvan, A. & Fernandez, E. 2004. Transgenic microalgae as green cellfactories. Trends Biotechnol. 22:45-52. DOI |
16 | Lumbreras, V., Stevens, D. R. & Purton, S. 1998. Efficient foreign gene expression in Chlamydomonas reinhardtii mediated by an endogenous intron. Plant J. 14:441-447. DOI |
17 | Gonzalez-Ballester, D., de Montaigu, A., Higuera, J. J., Galvan, A. & Fernandez, E. 2005. Functional genomics of the regulation of the nitrate assimilation pathway in Chlamydomonas. Plant Physiol. 137:522-533. DOI |
18 | Folda, A., Citta, A., Scalcon, V., Calì, T., Zonta, F., Scutari, G., Bindoli, A. & Rigobello, M. P. 2016. Mitochondrial thioredoxin system as a modulator of cyclophilin D redox state. Sci. Rep. 6:23071. DOI |
19 | Grabherr, M. G., Haas, B. J., Yassour, M., Levin, J. Z., Thompson, D. A., Amit, I., Adiconis, X., Fan, L., Raychowdhury, R., Zeng, Q., Chen, Z., Mauceli, E., Hacohen, N., Gnirke, A., Rhind, N., di Palma, F., Birren, B. W., Nusbaum, C., Lindblad-Toh, K., Friedman, N. & Regev, A. 2011. Fulllength transcriptome assembly from RNA-Seq data without a reference genome. Nat. Biotechnol. 29:644-652. DOI |
20 | Harris, E. H. 1989. The Chlamydomonas sourcebook: a comprehensive guide to biology and laboratory use. Academic Press, San Diego, CA, 780 pp. |
21 | Bolger, A. M., Lohse, M. & Usadel, B. 2014. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30:2114-2120. DOI |
22 | Cheng, X., Liu, G., Ke, W., Zhao, L., Lv, B., Ma, X., Xu, N., Xia, X., Deng, X., Zheng, C. & Huang, K. 2017. Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas. Plant Methods 13:36. DOI |
23 | Dent, R. M., Haglund, C. M., Chin, B. L., Kobayashi, M. C. & Niyogi, K. K. 2005. Functional genomics of eukaryotic photosynthesis using insertional mutagenesis of Chlamydomonas reinhardtii. Plant Physiol. 137:545-556. DOI |
24 | Engler-Blum, G., Meier, M., Frank, J. & Muller, G. A. 1993. Reduction of background problems in nonradioactive northern and southern blot analyses enables higher sensitivity than 32P-based hybridizations. Anal. Biochem. 210:235-244. DOI |
25 | Ochman, H., Gerber, A. S. & Hartl, D. L. 1988. Genetic applications of an inverse polymerase chain reaction. Genetics 120:621-623. |
26 | Erjavec, N., Bayot, A., Gareil, M., Camougrand, N., Nystrom, T., Friguet, B. & Bulteau, A. L. 2013. Deletion of the mitochondrial Pim1/Lon protease in yeast results in accelerated aging and impairment of the proteasome. Free Radic. Biol. Med. 56:9-16. DOI |
27 | Merchant, S. S., Prochnik, S. E., Vallon, O., Harris, E. H., Karpowicz, S. J., Witman, G. B., Terry, A., Salamov, A., Fritz-Laylin, L. K., Maréchal-Drouard, L., Marshall, W. F., Qu, L. -H., Nelson, D. R., Sanderfoot, A. A., Spalding, M. H., Kapitonov, V. V., Ren, Q., Ferris, P., Lindquist, E., Shapiro, H., Lucas, S. M., Grimwood, J., Schmutz, J., Cardol, P., Cerutti, H., Chanfreau, G., Chen, C. -L., Cognat, V., Croft, M. T., Dent, R., Dutcher, S., Fernandez, E., Ferris, P., Fukuzawa, H., Gonzalez-Ballester, D., Gonzalez-Halphen, D., Hallmann, A., Hanikenne, M., Hippler, M., Inwood, W., Jabbari, K., Kalanon, M., Kuras, R., Lefebvre, P. A., Lemaire, S. D., Lobanov, A. V., Lohr, M., Manuell, A., Meier, I., Mets, L., Mittag, M., Mittelmeier, T., Moroney, J. V., Moseley, J., Napoli, C., Nedelcu, A. M., Niyogi, K., Novoselov, S. V., Paulsen, I. T., Pazour, G., Purton, S., Ral, J. -P., Riano-Pachón, D. M., Riekhof, W., Rymarquis, L., Schroda, M., Stern, D., Umen, J., Willows, R., Wilson, N., Zimmer, S. L., Allmer, J., Balk, J., Bisova, K., Chen, C. -J., Elias, M., Gendler, K., Hauser, C., Lamb, M. R., Ledford, H., Long, J. C., Minagawa, J., Page, M. D., Pan, J., Pootakham, W., Roje, S., Rose, A., Stahlberg, E., Terauchi, A. M., Yang, P., Ball, S., Bowler, C., Dieckmann, C. L., Gladyshev, V. N., Green, P., Jorgensen, R., Mayfield, S., Mueller-Roeber, B., Rajamani, S., Sayre, R. T., Brokstein, P., Dubchak, I., Goodstein, D., Hornick, L., Huang, Y. W., Jhaveri, J., Luo, Y., Martinez, D., Ngau, W. C. A., Otillar, B., Poliakov, A., Porter, A., Szajkowski, L., Werner, G., Zhou, K., Grigoriev, I. V., Rokhsar, D. S. & Grossman, A. R. 2007. The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science 318:245-250. DOI |
28 | Moellering, E. R. & Benning, C. 2010. RNA interference silencing of a major lipid droplet protein affects lipiddroplet size in Chlamydomonas reinhardtii. Eukaryot. Cell 9:97-106. DOI |
![]() |