• Journal of Pharmacopuncture
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• v.15 no.2
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• pp.7-10
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• 2012

Objectives: The root of Lithospermum erythrorhizon Sieb. et Zucc. (Lithospermi Radix, LR) is a kind of heat clearing and blood cooling medicinal herbs. It can clear away heat and cool the blood, reduce toxins and disperse maculae. LR has long been used as efficacious therapy for inflammation, burns, frostbite and skin diseases such as eczema and psoriasis. Methods: In the present study, we investigate anti-allergic and anti-inflammatory effects of LR by using the 1-fluoro-2, 4- dinitrofluorobenzene (DNFB)-induced contact dermatitis mouse model. Results: Topical application of 10 mg/mL of LR effectively inhibited skin lesions induced by repeated paintings with DNFB. Topical application of LR also inhibited hyperplasia, edema, spongiosis and infiltrations of mononuclear cells. In addition, production levels of total immunoglobulin and IgG1 in serum were decreased by using LR in vivo. Conclusions: These data suggest that LR acts as an antiinflammatory agent, improving skin lesions in CD mice.

• Journal of Environmental Health Sciences
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• v.47 no.6
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• pp.513-520
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• 2021

Background: Benzalkonium chloride (BKC) has been extensively used as a preservative in industrial products and in hygiene, medical, and cosmetic applications. Humidifier disinfectant (HD) products containing BKC have been used in South Korea. Objectives: This study was aimed to review types of products containing BKC, to summarize the regulations in the US, EU, Japan, and South Korea, and to review the health effects associated with the use of HD. Methods: We reviewed and summarized documents which were searched through PubMed and Google Scholar with the key words: BKC and asthma/contact dermatitis, humidifier disinfectant-associated lung injury (HDLI), and more. Results: Regulations in most countries including South Korea do not allow its use as disinfectants in spray-type of products for medicine, cosmetics, and household products. Two types of HD containing BKC (800~1,270 ppm) were marketed in South Korea from 1996~2003. Health effects reported from people who used products containing BKC were allergic contact dermatitis, erythema, and respiratory disease, including asthma. Two people who responded as HD users containing BKC only were confirmed to have developed asthma. HD-associated lung injury (HDLI) was reported by consumers who used both HD containing polyhexamethylene guanidine (PHMG) and HD containing BKC. Conclusions: In conclusion, the use of BKC as a biocide has to be controlled considering the route and pattern of exposure. Products containing BKC as preservatives were reviewed with exposure routes and sites in the human body such as skin, eyes, and the respiratory tract. HD containing BKC was clinically evaluated to be associated with asthma.

• Biomolecules & Therapeutics
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• v.32 no.1
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• pp.136-145
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• 2024

People with obesity maintain low levels of inflammation; therefore, their exposure to foreign antigens can trigger an excessive immune response. In people with obesity or allergic contact dermatitis (ACD), symptoms are exacerbated by a reduction in the number of regulatory T cells (Tregs) and IL-10/TGF-β-modified macrophages (M2 macrophages) at the inflammatory site. Benefits of intermittent fasting (IF) have been demonstrated for many diseases; however, the immune responses regulated by macrophages and CD4⁺T cells in obese ACD animal models are poorly understood. Therefore, we investigated whether IF suppresses inflammatory responses and upregulates the generation of Tregs and M2 macrophages in experimental ACD animal models of obese mice. The IF regimen relieved various ACD symptoms in inflamed and adipose tissues. We showed that the IF regimen upregulates Treg generation in a TGF-β-dependent manner and induces CD4⁺T cell hypo-responsiveness. IF-M2 macrophages, which strongly express TGF-β and inhibit CD4⁺T cell proliferation, directly regulated Treg differentiation from CD4⁺T cells. These results indicate that the IF regimen enhances the TGF-β-producing ability of M2 macrophages and that the development of Tregs keeps mice healthy against ACD exacerbated by obesity. Therefore, the IF regimen may ameliorate inflammatory immune disorders caused by obesity.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.15 no.1
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• pp.76-95
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• 2002

Allergic rhinitis and allergic dermatitis are common allergic diseases. Galgeun-tang has been used for treating various diseases, which include allergic rhinitis and allergic dermatitis. Experimental sutdies have been done to research the anti-allergic effects of Galgeun-tang, Gamigalgeun-tang and Geomahwanggalgeun-tang. We have observed: the vascular permeability response induced by serotonin and histamine, the contact dermatitis response induced by picryl chloride, the delayed type hypersensitivity response to SRC, the mice paw edema induced by carrageenin, and the rectal temperature in febrile rats induced by endotoxin, as well as the writhing syndrome induced by $0.7{\%}$ acetic acid. The results were as follows: 1. In the vascular permeability response to intradermal serotonin and histamine, Galgeun-tang and Gamigalgeun-tang showed a significant inhibitory effect. However Geomahwanggalgeun-tang showed an insignificant inhibitory effect. 2. In the contact dermatitis response induced by picryl chloride, Galgeun-tang and Gamigalgeun-tang showed a signigicant inhibitory effect. However Geomahwanggalgeun-tang showed an insignificant inhibitory effect. 3. In the delayed type hypersensitivity response to SRC, Galgeun-tang, Gamigalgeun-tang and Geomahwanggalgeun-tang showed a significant inhibitory effect. 4. In the mice paw edema induced by carrageenin, Galgeun-tang and Gamigalgeun-tang showed a significant inhibitory effect. However Geomahwanggalgeun-tang showed an insignificant inhibitory effect. 5. In the rectal temperature in febrile rats induced by endotoxin, Galgeun-tang, Gamigalgeun-tang and Geomahwanggalgeun-tang showed a significant inhibitory effect. 6. In the writhing syndrome induced by a $0.7{\%}$ acetic acid solution. Galgeun-tang and Gamigalgeun-tang showed a significant inhibitory effect. However Geomahwanggalgeun-tang showed an insignificant inhibitory effect.

• The Korea Journal of Herbology
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• v.23 no.2
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• pp.59-65
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• 2008

Objectives : HRHDT has been known as a useful prescription with antibiotic, anti-inflammatory, antioxidative and immunosuppressive activity. To evaluate anti-atopic dermatitis effect of HRHDT, we treated HRHDT in NC/Nga mice model skin induced contact hypersensitivity. Methods : Contact hypersensitivity, a local inflammatory response of skin, was induced by spreading the back skin of NC/Nga mice with 0.4${\sim}$1% DNCB. HRHDT was prepared by dissolving 3% HRHDT in solution and treated 3 weeks on the back skin. Results : HRHDT significantly reduced TEWL and erythema by 0.4${\sim}$1% of DNCB treatment compared to control group. HRHDT also reduced IgE on serum obtained from blood of DNCB-treated mice. Conclusions : These results showed that HRHDT could be used as a pharmaceutical material with anti-atopic dermatitis effect by reducing IgE in contact, hypersensitivity atopic dermatitis NC/Nga mice model by DNCB.

• Journal of Preventive Medicine and Public Health
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• v.32 no.2
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• pp.206-214
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• 1999

Objectives: This study was carried out to identify relation of health care utilization due to skin disease(ICD-10, L00-L99) and characteristics(sex, age, exposure status, type of industry, size of enterprise) of workers. Methods: We made new database composed of 30,536 workers' health examination results in one specific health examination institute in Inchon and data of medical insurance utilization due to skin disease in 4 medical insurance associations for enterprise from January, 1995 to December, 1997 And we analyzed determinants of health care utilization due to skin disease of workers. Results: Among 30,536 study subjects, 8,837(28.9%) workers and 4,181 (13.7%) workers utilized medical insurance due to total skin disease(ICD-10, L00-L99) and contact dermatitis(ICD-10, L23-L25), respectively. Female workers(p<.001), workers exposed to organic solvents(p<.05), workers if manufacturing industries(p<.05, p<.01, respectively) and in large scale enterprises(p<.001) utilized more medical insurance due to total skin disease and contact dermatitis than male workers, workers not exposed to organic solvents, workers in non-manufacturing industries and small scale enterprises. With multiple logistic regression analysis, significant explanatory variables affecting workers' medical utilization due to total skin disease and dermatitis and eczema(ICD-10, L20-L30) in total workers were sex, age, specific chemicals and size of enterprises. And age, type of industries, organic solvents, specific chemicals and size of enterprises were significant explanatory variables related to medical insurance utilization due to dermatitis and eczema and contact dermatitis in male workers. Conclusion: From the above results, we found that workers exposed to organic solvents utilized more medical insurance due to skin disease than workers not exposed to. And, comparing to workers in large scale enterprises, workers in small scale enterprises may have unmet medical care utilization due to skin disease. Therefore we have to establish counterplan to manage occupational skin disease of high risk group(organic solvent exposure group) and to satisfy unmet medical care utilization of workers in small scale enterprises.

• Journal of Preventive Medicine and Public Health
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• v.30 no.4 s.59
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• pp.830-839
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• 1997

Painting is risky work to occupational skin disease. This research was carried out to investigate the prevalence of occupational skin diseases in painting department of a shipyard company in June 1996. Dermatological examination, self-administered questionnaire survey were conducted. Exposed group(n=379) was selected randomly in painting department and control of group(n=151) was selected in those who had not exposed to paints or solvents. The prevalence of contact dermatitis(11.9%) is significantly elevated compared with control of group(2.6%), and odds ratio(OR) is 4.95 (95% confidence interval[CI]: 1.75-14.02). The prevalence of Tinea pedis, the most common skin disease, in exposure group is 48.0%, and its odds ratio(OR) is significantly elevated compared with control group (OR:3.18, 95% CI: 2.06-4.90). Pompholyx is also significantly elevated in prevalence(11.9%) and OR(OR:6.64, 95% CI:2.03-21.69). There were no difference in the prevalence of contact dermatitis, Tinea pedis, and pompholyx by use of personal protective equipments(PPE), job categories, and duration of employment in painting department. In exposure group, 71.1% suffer from contact dermatitis improved in vacation or holiday and 68.9% of them had lesion on exposed area, it suggest that contact dermatitis in exposed group is related to their work. In conclusion, workers in painting department have high risks of contact dermatitis, Tinea pedis, and pompholyx. A health policy should be provided to prevent skin disease among painting department.

• Journal of Veterinary Clinics
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• v.31 no.3
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• pp.180-193
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• 2014

Allergic contact dermatitis (ACD) is an inflammatory skin disease and regarded as a prototype of T-cell mediated delayed-type hypersensitivity reactions. Aroma Oil Complex (AOC) is composed of lavender true oil, chamomile roman oil and tea tree oil. This study was performed to assess the effects of AOC in a canine model of ACD. ACD was induced on the back of dogs induced by sensitization and repeated application by 2,4-dinitro-1-chlorobenzene (DNCB). Topical treatment of AOC was applied once a day for 8 days and skin biophysical parameters including transepidermal water loss (TEWL), skin hydration, skin thickness and erythema index, were measured every two days during experimental periods. Histopathology and immunohistochemistry were performed to evaluate the anti-inflammatory effect. In skin biophysical parameters, TEWL, skin hydration, skin thickness and erythema index were significantly increased, with a maximum increase appeared on day 2 (p<0.05). After the completion of AOC treatment, skin biophysical parameters were significantly reached those of baseline in a time-dependent manner (p<0.05). In histopathology, marked increases of epidermal thicknesses were induced after DNCB challenge with numerous inflammatory cell infiltrations and edematous changes, decreases of connective tissue occupied regions in dermis. In addition, marked increases of cytokine - tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interferon-${\gamma}$ (IFN-${\gamma}$)-immunoreactivities in the dermis and of apoptotic markers - caspase-3 and PARP-immunoreactivities in the epidermis were observed in DNCB control as compared with intact control, respectively (p<0.01). The decrease of infiltrated inflammatory cells and related decreases of pro-inflammatory cytokine immunoreactivities were observed in AOC treated skin (p<0.01). Based on these findings, AOC may have anti-inflammatory and alleviatory effects in the allergic contact dermatitis.

• Journal of Life Science
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• v.21 no.5
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• pp.713-719
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• 2011

In this study, we evaluated whether Lunasin Enriched Soybean (LES) suppressed 1-chloro-2,4-dinitrochlorobenzene (DNCB) induced allergic contact dermatitis in vivo. In the group treated with DNCB alone, erythema and hemorrhage in the skin were observed, while the application of LES after DNCB treatment modulated erythema and hemorrhage similar to the untreated group. In histopathological analysis of the skin, DNCB-treated groups showed increased skin thickness compared to the untreated groups, while the treatment of LES dose-dependently modulated skin thickness. Also, hypertrophy and hyperkeratosis of the epidermis, intracellular edema and infiltration of inflammatory cells were observed in the group treated with DNCB alone, while treatment of LES inhibited these inflammatory changes. DNCB also induced a significant up-regulation of iNOS and COX-2 expression, as compared to the untreated groups. However, the treatment of LES significantly decreased the down-regulation of iNOS and COX-2 expression, as compared to DNCB-treated groups. Also, LES reduced the IgE level in DNCB-stimulated groups. And lunasin from LES translocated the nucleus of the cells in the skin of Sprague-Dawley rats. Taken together, it is thought that LES has the potential to be used as an alternative treatment for allergic contact dermatitis.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.11 no.1
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• pp.197-218
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• 1998

In this study, I invastigate about oriental medical drug, dosage form and directions in external therapy of contact dermatitis. and after study on relationship oriental medical drug, dosage form and directions with cause, symptom and differentiation of symptom and sign. The results are as follows; 1. Most frequently used oriental medical drug is until qing(淸) dynasty Eriocheir sinensis H. Milne-Edwards(蟹), Salix babylonica L.(柳葉), Natrii sulfas(芒硝), Allium macrostemon Bge.(해), Nelumbo nucifera Gaertn.(荷葉), nowadays in Korea and China Phellodendron amurense Rupr.(黃柏), Gypsum(石膏), Rheum palmatum L.(大黃), Baphicacanthus cusia Bremek(靑黛), Talcum(滑石). 2. In the frequency of dosage form, until qing(淸) dynasty powder 1case, liquor 49cases, liquer and solid mixture 58cases, nowadays Korea and China powder 16cases, liquor 96cases, liquer and solid mixture 59cases. 3. Most frequently used directions of dosage is thinly attaching method(薄貼法), attaching method(敷貼法), furnigating and cleansing method(熏洗法), cleansing method(洗傷法), wet dressing method(濕敷法), spreading powder method(撲粉法), plaster method(途차法), rubbing skin method(摩擦法) 4. In the external therapy of contact dermatitis, oriental medical drug's usage is based on stage of contact dermatitis In acute stage, most frequently used drug is heat and damp remove drug(淸熱燥濕藥), nature of drug(藥性) is bitter taste and cold charactor(苦寒), In chronic stage, most frequently used drug is nourishing the blood drug(養血藥), promoting blood circulation drug(活血藥). 5. The dosage form of drug is based on symptom. In acute stage, when papules and vesicles, or erosion and exudation is the main symptom of skin, liquor or powder is used, when erosion and crust is the main symptom of skin, plaster is used. In chronic stage, plaster is used. 6. In the directions of dosage is based on dosage form of drug and symptom. In acute stage, when papules and vesicles is the main symptom of skin, fumigating and cleansing method, cleansing method, plaster method is used, when erosion, vesicles and exudation the main symptom of skill, cleansing method, wet dressing method, spreading powder method, attaching method, spreading powder method is used, when crust is the main symptom of skin, plaster method is used. In chronic stage, plaster method, rubbing skin method is used.