• Journal of the Korean Society of Hazard Mitigation
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• v.11 no.2
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• pp.45-52
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• 2011

To improve fire-resistance of a high strength concrete against spalling under elevated temperature, fibers can be mixed to provide flow paths of evaporated water to the surface of concrete when heated. In this study, the experiment of a column under fire and mechanical loads is conducted and the material model for predicting temperature of reinforcement steel bar and mechanical behavior of fiber-mixed high strength concrete is suggested. The material model in previous studies is modified by incorporating physical behavior of internal concrete and thermal characteristics of concrete at the elevated temperature. Thermo-mechanical analysis of the fiber-mixed high strength concrete column is conducted using the calibrated material model. The performance of the proposed material model is confirmed by comparing thermo-mechanical analysis results with the experiment of a column under fire and mechanical loads.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.545-549
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• 2003

After irradiation of a cloned dextransucrase gene (dsrB742) with ultrasoft X-ray, an E. coli transformant (pDSRB742CK) was first developed for the expression of an extracellular dextransucrase, having increased activity and the synthesis of a highly branched dextran. Seven nucleotides of the parent gene (dsrB742) were changed in the nucleotide sequences of dsrB742ck. Among them, four nucleotides were changed at the ORF of dsrB742, resulting in a 30 amino acids deletion in the N-terminal of DSRB742 dextransucrase. The activity of DSRB742CK dextransucrase in culture supernatant was approximately 2.6 times higher (0.035 IU/ml) than that of the DSRB742 clone. The pDSRB742CK clone produced DSRB742CK dextransucrase when grown both on a sucrose medium (inducibly) and on a glucose medium (constitutively). The DSRB742 clone did not produce dextran constitutively on a glucose medium. DSRB742CK dextran had 15.6% branching and 2.7-times higher resistance to dextranase hydrolysis compared to DSRB742 dextran. $^{13}C-NMR$ showed that DSRB742CK dextran contained ${\alpha}-(1{\rightarrow}3)$ branch linkages that were not present in DSRB742 dextran.

• Journal of Applied Biological Chemistry
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• v.51 no.2
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• pp.50-56
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• 2008

As one way to approach to cold defense mechanism in plants, we previously identified the gene for protein-tyrosine phosphatase (CaTPP1) from hot pepper (Capsicum annuum) using cDNA microarray analysis coupled with Northern blot analysis. We showed that the CaTPP1 gene was strongly induced by cold, drought, salt and ABA stresses. The CaTPP1 gene was engineered under control of CaMV 35S promoter for constitutive expression in transgenic tobacco plants by Agrobacterium-mediated transformation. The resulting CaTPP1 transgenic tobacco plants showed significantly increased cold stress resistance. It also appeared that some of the transgenic tobacco plants showed increased drought tolerance. The CaTPP1 transgenic plants showed no visible phenotypic alteration compared to wild type plants. These results showed the involvement of protein tyrosine phosphatase in tolerance of abiotic stresses including cold and drought stress.

• Computers and Concrete
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• v.18 no.5
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• pp.999-1018
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• 2016

Enhanced tensile properties of fiber reinforced concrete make it suitable for strengthening of reinforced concrete elements due to their superior corrosion resistance and high tensile strength properties. Recently, the use of fibers as strengthening material has increased motivating the development of numerical tools for the design of this type of intervention technique. This paper presents numerical analysis results carried out on a set of concrete beams reinforced with short fibers. To this purpose, a database of experimental results was collected from an available literature. A reliable and simple three-dimensional Finite Element (FE) model was defined. The linear and nonlinear behavior of all materials was adequately modeled by employing appropriate constitutive laws in the numerical simulations. To simulate the fiber reinforced concrete cracking tensile behavior an approach grounded on the solid basis of micromechanics was used. The results reveal that the developed models can accurately capture the performance and predict the load-carrying capacity of such reinforced concrete members. Furthermore, a parametric study is conducted using the validated models to investigate the effect of fiber material type, fiber volume fraction, and concrete compressive strength on the performance of concrete beams.

• Structural Engineering and Mechanics
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• v.4 no.3
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• pp.257-276
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• 1996

In North America, a large number of concrete old slab-on-steel girder bridges, classified noncomposite, were built without any mechanic connections. The stablizing effect due to slab/girder interface contact and friction on the steel girders was totally neglected in practice. Experimental results indicate that this effect can lead to a significant underestimation of the load-carrying capacity of these bridges. In this paper, the two major components-concrete slab and steel girders, are treat as two deformable bodies in contact. A finite element procedure with considering the effect of friction and contact for the analysis of concrete slab-on-steel girder bridges is presented. The interface friction phenomenon and finite element formulation are described using an updated configuration under large deformations to account for the influence of any possible kinematic motions on the interface boundary conditions. The constitutive model for frictional contact are considered as slip work-dependent to account for the irreversible nature of friction forces and degradation of interface shear resistance. The proposed procedure is further validated by experimental bridge models.

• Journal of the Korean Society for Heat Treatment
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• v.5 no.4
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• pp.201-208
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• 1992

An experimental study of the constitutive response of precipitation-strengthened Al-0.55wt% Zr alloy, which consists of an Al matrix precipitation-strengthened by coherent particles, ${\beta}^{\prime}(Al_3Zr)$ with $L1_2$ structure has been performed. The deformation response of the materials has been examined by stress relaxation test at 573K, 623K and 673K. It was found that there exist the threshold stress during stress relaxation and threshold stress results from the presense of ${\beta}^{\prime}(Al_3Zr)$ particles. The ratio of threshold stress and Orowan stress decreased gradually with increasing temperature. The resistance to climb-pass of particles was independent of particles size for a fixed volume fraction although the threshold for bowing and particles cutting are sensitive to the particles dimensions. The smaller particles cutted by dislocations. This behavior of dislocations in this alloy was explained in terms of the small value antiphase boundary energy. The dislocation networks wrere more extensive in spesimens subjected to stress relaxation and there were numerous areas that have a high denstiy of jogged dislocation. This experiment results indicate that the rate controlling stress relaxation process is the climb of edge dislocation over particles.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.11a
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• pp.135-139
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• 1996

$p70^{s6k}$ lies on a $p21^{ras}$-independent signalling pathway and plays an important role in mitogenesis. Activation is associated with phosphorylation at multiple sites, four of which lie in an autoinhibitory region. The immunosuppressant rapamycin induces $p70^{s6k}$ inactivation through dephosphorylation of a second set of mitogen-induced sites. Here we identify these sites as $T_{229}$, $T_{389}$, and $S_{404}$. $T_{229}$ resides in the "T loop" of the catalytic domain, an essential phosphorylation site in other kinases. However, $p70^{s6k}$ inactivation by rapamycin most closely parallels $T_{389}$ dephosphorylation. Mutation of $T_{389}$ to alanine ablates kinase activity, whereas mutation to glutamic acid confers constitutive kinase activity and rapamycin resistance. indicating an essential role for phosphorylation at this site. $T_{389}$ resides in an unusual hydrophobic motif, not previously noted, between the catalytic and autoinhibitory domains. The importance of this site, and surrounding motif, is emphasized by its conservation in other kinases including homologues of $p70^{s6k}$ derived from such distantly related organisms as yeast and plant.

• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2004.05a
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• pp.27-29
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• 2004

Drug transporters play an essential role in the absorption, distribution and elimination of clinical drugs, nutrients and toxicants. The importance of the transporters is exampled by therapeutic failure in cancer chemotherapy that is mainly caused by the overexpression of multidrug resistance (MDR)-related transporters. In addition, the transporters may involve in drug-drug interactions that lead to serious adverse drug responses and some transporters also contribute to inter-individual variation in drug responses. As an effort to understand the mechanism underlying the inter-individual variation of transporters activity, genetic and environmental factors influencing the expression or function of the transporters have extensively explored through last decade. Among them, genetic polymorphism of drug transporter encoding genes has generated much interest since the discovery of functional single nucleotide polymorphisms (SNP) of MDR1 gene. Besides drug transporters, xenobiotic receptors also modulate drug disposition by regulating the transcription of drug metabolizing enzymes and drug transporters. Among many xenobiotic receptors, pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are two most well characterized since these receptors show wide substrate specificities and regulate the expression of various enzymes involved in drug disposition. Recently, several functional genetic polymorphisms were reported in PXR coding gene. In the present study, genetic polymorphisms of two drug transporters, MDR1 and BCRP, and two xenobiotic receptors, PXR and CAR, were investigated in Korean population.

• Journal of Korean Physical Therapy Science
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• v.9 no.4
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• pp.45-52
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• 2002

Rats that are fed a fructose-rich diet develop hypertension, insulin resistance, and hypertriglyceridemia. To elucidate whether altered expression levels of endothelin-1 and nitric oxide synthase are related to the development of insulin-resistant hypertension, we examined the present study. Male Sprague-Dawley rats were fed a fructose-rich diet for 5 weeks. Systolic blood pressure significantly increased in fructose-fed rats. While serum free fatty acid and plasma nitrite/nitrate levels did not significantly differ between the fructose-fed and control groups, plasma insulin and serum triglyceride concentrations significantly increased in the former. Endothelin-1 mRNA expression in the aorta increased in fructose-fed rats. Neither the protein expression of constitutive nitric oxide synthase nor that of inducible nitric oxide synthase were significantly affected by fructose feeding. However, nitrite/nitrate levels in the aorta were significantly increased. These results suggest that an increase in vascular endothelin-1 is an important contributing factor to the development of hypertension in fructose-fed rats. However, the vascular nitric oxide pathway may not be causally related to the development of fructose-induced hypertension.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2005.05a
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• pp.275-278
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• 2005

INCONEL 718, nickel based superalloy, has good formability, high strength, excellent corrosion resistance and mechanical properties at high temperature. Owing to theses attractive properties, it finds use in applications such as combustion system, turbine engines and nuclear reactors. In such applications, components are typically required to be tolerant of high stress impact loading. This may cause material degradation and lead to catastrophic failure during service operation. In order to design optimal structural parts made of INCONEL 718, accurate understanding of material's mechanical properties, dynamic behavior and fracture characteristic as a function of strain rates are required. This paper concerned with the dynamic material properties of the INCONEL 718 for the various strain rates. The dynamic response of the INCONEL 718 at intermediate strain rate is obtained from the high speed tensile test machine test and at the high strain rate is from the split Hopkinson pressure bar test. Based on the experimental results, the effects of strain rate on dynamic flow stress, work hardening characteristics, strain rate sensitivity and elongation to the failure are evaluated. Experimental results from both quasi-static and high strain rate up to the 5000/sec are interpolated in order to construct the Johnson-Cook model as the constitutive relation that should be applied to simulate and design the structural parts made of INCONEL 718.