• Journal of Life Science
• /
• v.33 no.8
• /
• pp.623-631
• /
• 2023

This study was conducted to develop microsatellite markers in Parapristipoma trilineatum using next-generation sequencing. A total of 402,244,934 reads were generated on the Illumina Hiseq X Ten System, yielding 60,738,985,034 bp of sequences. The de novo assembly resulted in 1,320,995 contigs. A total of 952,326 contigs (0.016%) including 151 microsatellite loci were derived from the 1,320,995 contigs longer than 640 bp. A total of 34 primer sets were designed from the 151 microsatellite loci. As a result, 15 microsatellite loci were chosen and used for assuming population genetic parameters in the wild and farmed populations. The mean number of effective alleles was 12, ranging from 6 to 25. The observed heterozygosity (H_O) and the expected heterozygosity (H_E) ranged between 0.530 and 0.873, with an average of 0.750, and from 0.647 to 0.895, with an average of 0.793, respectively. According to these results, the developed set of 15 microsatellite markers is expected to be useful for the analysis of genetic characteristics in the population of P. trilineatum in Korea. There are requirements now for further genetic information, fishery resource management, breeding guidelines, support with the selection of breeds and studies on the effects of release, all of which will improve species conservation, and through future research, we aim to offer genetic foundational data with that goal.

• Korean Journal of Ecology and Environment
• /
• v.56 no.4
• /
• pp.375-383
• /
• 2023

To determine the cause of the population decline in Gobiobotia naktongensis, substrate preference and burying behaviour were investigated in this study. In general, the species was shown to prefer a substrate size of 1 mm or less, depending on the flow. In addition, the burying depth varied according to the size of the fish and increased with a decrease in water temperature. Our findings showed that the main cause of the population reduction was the physical changes in the substrate structure due to the dams or barrages construction. Notably, the accumulation of silt and mud in the substrate upon the formation of an upstream lentic water region for structural construction and bed armouring caused by scouring and reduced downstream inflow of fine sediment were deterministic in the fish habitat changes, causing problems in burying. As sand substrate structure is critical for the survival and inhabitation of psammophilous species, efficient strategies should be developed with proper habitat management to reduce the anthropogenic damage

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.13 no.2
• /
• pp.129-139
• /
• 2008

Marine ecotoxicological standard method was applicated using marine rotifer and benthic copepod as primary consumer of marine ecosystem. Marine rotifer, Brachionus plicatilis and benthic copepod, Tigriopus japonicus were designated as standard test species with the endpoints of 24hr neonate mortality (24hr $LC_{50}$) and 48hr population growth (48hr $EC_{50}$) for rotifer, and 48hr nauplius mortality (48hr $LC_{50}$) for benthic copepod. Tests method was referred to those of ASTM (American Society for Testing and Materials) with the replacement of test species which are widely distributed in Korean waters. The two species showed a wide tolerance on salinity ($5{\sim}35\;psu$) and can be easily cultured in small space even they were not as sensitive as in the mortality test using the nauplius of marine invertebrates. However, these species revealed the significant "concentrationresponse relationship" tested with ocean disposal wastes, and reproducibility using cadmium as standard reference material between laboratories. Accordingly, these two species have good potential as test species for marine ecotoxicological test species. Also, we strongly recommend that at least one of these species be included in the test species of "battery test" in marine bioassay.

• Animal cells and systems
• /
• v.14 no.1
• /
• pp.37-44
• /
• 2010

Black sea bream, Acanthopagrus schlegelii, is a commercially important fish in Korea. As a preliminary investigation into the effect of hatchery rearing for stock enhancement, we examined genetic diversity between wild and farmed black sea bream populations from Jeju using six microsatellite markers. High levels of polymorphism were observed between the two populations. A total of 87 different alleles were found at the loci, with some alleles being unique. Allelic variability ranged from 8 to 22 in the wild population and from 7 to 17 in the farmed one. Average observed and expected heterozygosities were estimated at 0.87 and 0.88 in the wild sample. The corresponding estimates were 0.83 and 0.86 in the farmed sample. Although a considerable loss of rare alleles was observed in the farmed sample, no statistically significant reductions were found in heterozygosity or allelic diversity in the farmed sample, compared with the wild one. Significant genetic heterogeneity was found between the wild and farmed populations. These results suggest that more intensive breeding practices for stock enhancement may have resulted in a further decrease of genetic diversity. Thus, it is necessary to monitor genetic variation in bloodstock, progeny, and target populations and control inbreeding in a commercial breeding program for conservation. This information may be useful for fisheries management and the aquaculture industry.

• Safety and Health at Work
• /
• v.6 no.2
• /
• pp.134-138
• /
• 2015

Background: Noise-induced hearing loss (NIHL) is a major preventable occupational health problem with 250 million people worldwide known to have disabling impairment of moderate to greater severity. The aims of the study are to estimate the prevalence of NIHL in the police force; and study its association with age, sex, duration of service (years), smoking and alcohol habits, use of hearing protective devices, as well as preexisting chronic diseases. Methods: A cross-sectional study was conducted on 543 police personnel who had undergone periodic medical examination over a 12-month period. The diagnostic criteria for NIHL were (1) history of occupational noise exposure, (2) bilateral hearing loss, (3) hearing loss of ${\geq}25dBA$ at 4,000 Hz in two consecutive audiograms, and (4) no significant medical history affecting hearing. Severity of NIHL was based on the World Health Organization grading. Results: Males (74.8%) made up the majority of the police force. The mean age for police personnel was $35.55{\pm}9.57years$, and the mean duration of service was $14.75{\pm}9.39years$. Compliance with the usage of hearing protective devices was seen in 64.4%. The prevalence of NIHL in this study population was 34.2%, with a higher prevalence in males (37.7%) than in females (23.9%). The study also showed strong associations between NIHL and male sex (odds ratio, 1.9; P < 0.05), and hypertension (odds ratio, 3.3; P < 0.001). Overall, 93% were found to have mild NIHL, 3.5% had moderate NIHL, and 3.5% had severe NIHL. No police personnel were found to have profound hearing loss. Conclusion: The prevalence of NIHL in this study is high compared to other similar studies among police personnel. This study shows that increasing age, male, presence of hypertension, diabetes, and longer duration of service are significant associated factors for NIHL. Preventative strategies include health surveillance, implementation of a hearing conservation program, and legislation.

• Journal of Korean Society of Forest Science
• /
• v.90 no.4
• /
• pp.573-581
• /
• 2001

The purposes of this study are to estimate mean willingness to pay (WTP) for preventing forest fires by contingent valuation method (CVM), and to calibrate the variables affecting WTP. The forest fire prevention fund was utilized as a payment vehicle to elicit respondents' willingness to pay (WTP). A total of 500 adults who reside in Seoul Metropolitan area were selected by two-stage cluster sampling and conducted the face-to-face interview. The scenario was designed to meet the requirements for double-bounded dichotomous choice CVM. More than half of the respondents (64.6%) have a willing to pay for the fund. The mean WTP was \4,532. Therefore a total WTP for the population was \34,165,758,000. The calibration of Weibull proportional hazard model showed that education level, environmental conservation intention and negative consciousness about the effect of forest fire were independent variables strongly influencing the WTP.

• Asian-Australasian Journal of Animal Sciences
• /
• v.24 no.12
• /
• pp.1637-1643
• /
• 2011

Korean native chickens are a very valuable chicken population in Korea and their prices are higher than that of commercial broilers. In order to discriminate two commercial Korean native chicken populations (CCP1 and CCP2), single nucleotide polymorphisms (SNPs) from mitochondrial (mt) DNA D-loop sequences and LEI0258 marker polymorphisms in the major histocompatibility complex (MHC) region were investigated. A total of 718 birds from nine populations were sampled and 432 mtDNA sequences were obtained. Of these, two commercial Korean native chicken populations (363 birds) were used for investigation of their genetic relationship and breed differentiation. The sequence data classified the chickens into 20 clades, with the largest number of birds represented in clade 1. Analysis of the clade distribution indicated the genetic diversity and relation among the populations. Based on the mtDNA sequence analysis, three selected SNPs from mtDNA polymorphisms were used for the breed identification. The combination of identification probability (Pi) between CCP1 and CCP2 using SNPs from mtDNA and LEI0258 marker polymorphisms was 86.9% and 86.1%, respectively, indicating the utility of these markers for breed identification. The results will be applicable in designing breeding and conservation strategies for the Korean native chicken populations and also used for the development of breed identification markers.

• Asian-Australasian Journal of Animal Sciences
• /
• v.27 no.11
• /
• pp.1548-1553
• /
• 2014

Four Korean native cattle (KNC) breeds-Hanwoo, Chikso, Heugu, and Jeju black-are entered in the Domestic Animal Diversity Information System of the United Nations Food and Agriculture Organization (FAO). The objective of this study was to assess the genetic diversity, phylogenetic relationships and population structure of these KNC breeds (n = 120) and exotic breeds (Holstein and Charolais, n = 56). Thirty microsatellite loci recommended by the International Society for Animal Genetics/FAO were genotyped. These genotypes were used to determine the allele frequencies, allelic richness, heterozygosity and polymorphism information content per locus and breed. Genetic diversity was lower in Heugu and Jeju black breeds. Phylogenetic analysis, Factorial Correspondence Analysis and genetic clustering grouped each breed in its own cluster, which supported the genetic uniqueness of the KNC breeds. These results will be useful for conservation and management of KNC breeds as animal genetic resources.

• Journal of Korean Society on Water Environment
• /
• v.34 no.1
• /
• pp.67-76
• /
• 2018

There are 34 mega-cities with a population of more than 10 million in the world. One of the highly populated cities in the world is Seoul in South Korea. Seoul receives $1,140million\;m^3/year$ for domestic water, $2million\;m^3/year$ for agricultural water and $6million\;m^3/year$ for industrial water from multi-purpose dams. The maintenance water used for water conservation, ecosystem protection and landscape preservation is $158million\;m^3/year$, which is supplied from natural precipitation. Recently, the use of the other water for preservation of water quality and ecosystem protection in urban areas is increasing. The objectives of this study is to develop the seasonal forecast method of environmental water in urban areas (Seoul, Daejeon, Gwangju, Busan) and to evaluate its predictability. In order to estimate the seasonal outlook information of environmental water from Land Surface Model (LSM), we used the observation weather data of Automated Synoptic Observing System (ASOS) sites, forecast and hind cast data of GloSea5. In the past 30 years (1985 ~ 2014), precipitation, natural runoff and Urban Environmental Water Index (UEI) were analyzed in the 4 urban areas. We calculated the seasonal outlook values of the UEI based on GloSea5 for 2015 year and compared it to UEI based on observed data. The seasonal outlook of UEI in urban areas presented high predictability in the spring, autumn and winter. Studies have depicted that the proposed UEI will be useful for evaluating urban environmental water and the predictability of UEI using GloSea5 forecast data is likely to be high in the order of autumn, winter, spring and summer.

• Journal of Environmental Policy
• /
• v.10 no.3
• /
• pp.73-91
• /
• 2011

The fisheries in East Asia are reviewed in conjunction with climate change and social-economic developments in the 20th century. About one third of the human population resides in this region, producing a large share of the world's fisheries products, consuming them, and contributing significantly to the international trade of the products. Ongoing local and global climate changes, as well as ocean warming and acidification, are anticipated to have significant impacts on fisheries. Frequent typhoons have brought untold calamities and miseries to coastal communities. The rate of environmental change is outpacing our ability to respond effectively. The science must now move beyond identifying issues and toward providing sound bases for the development of innovative solutions, including effective adaptation and mitigation strategies. Fisheries management plans must be made to consider both changes in climate and social systems. It seems logical that an international forum should be made available to coordinate scientific research, management, and conservation of the region's fishery resources.