• Korean Chemical Engineering Research
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• 제46권4호
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• pp.722-726
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• 2008

실리카 microsphere는 HPLC를 위한 흡착 충진제와 같은 용도로 사용하기에 적합한 혁신적인 소재로 널리 알려져 있다. microsphere을 기능성고분자나 금속, 생리활성 물질과 같은 특정한 성질을 지닌 물질로 표면 개질시키므로 다양한 용도로 활용할 수 있다. 콜라겐은 생체조직을 구성하는 기본 단백질로 생체적합성이 뛰어난 물질로 주목받고 있는 기능성 소재이다. 본 연구에서는 50% 이상의 세공부피를 지닌 다공성 silica microsphere를 고분자 응집법인 PICA 법을 이용하여 colloidal silica로부터 제조하고 콜라겐 hydrogel을 사용하여 표면 개질시키므로 생체적합성을 증진시키는 방법을 연구하였다. 실리카/콜라겐 microsphere 에 은 나노입자를 담지시킨 microsphere 복합체를 만들고 특성을 조사하므로 생체소재로의 활용 가능성을 조사하였다.

• Journal of Oral Medicine and Pain
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• 제22권1호
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• pp.65-80
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• 1997

Cytochrome P450 is an oxidase involved in oxidation of alcohol and is known to be an activator of carcinogen. The present study was perfomed to analyze the effect of diabetes and cold stress on the expression of Cytochrome P450 IIE1(CYPIIE1) in the liver and salivary glands in rats by an immunoblot analysis. Sixty three divided into 4 groups; 1) 20 rats belonging to group I were allowed diabetes (40mg/kg. I.V.) 2) 20 rats of group II were bathed in cold water for 30 seconds twice a day 3) 20 rats comprising group III were received diabetes and cold stress as described above 4) 3 rats of group IV were selected as a control. The rats were sacrificed at the end of the same day 1, 2, 3, 4 weeks experiment. The liver and parotid glands were removed and stored at $-70^{\circ}C$ until use. The stored organs were homogenized for 10 seconds and the supernatants were obtained by centrifugation. The proteins of the supernatants were separated by sodium dodecyl sulfate- polyacrylamide gel electrophoresis and subjected to Western blotting. The blotted membranes were incubated with polyclonal antibodies to CYPIIE1. And sepimens were observed with light microscope also under the Hematoxillin-Eosin staining. The obtained results were as follows : 1. In diabetes group, acini had changed to degeneration severely 1 week experiment, but repaired gradually in lapse of time. 2. In diabetes group, septal connective tissue had changed to degeneration little by little from 1 week after experiment, and progressed severely in lapse of time. 3. In stress group, acini had not changed remarkably, but slightly separated each other 3 weeks after experiment. 4. In diabetes and stress group, histological feature had changed remarkably campare with in the group of diabetes only. 5. In all experimental group, CTPIIE1 had expressed remarkably in the liver tissue, but not in the parotid gland tissues. 6. In diabetes and stress group, CYPIIE1 had expressed remarkably campare with in the group of diabetes only.

• 구강회복응용과학지
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• 제19권3호
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• pp.139-151
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• 2003

This study was to evaluate and to compare the compressive strength and the displacement effecting the abutment or the residual ridge which are transformed by the angle and the heights of the konus denture inner crown when restorating the unilateral konus denture by using the mandibular canine and the 1st premolar as an abutment. The author made 9 different models for different inner crown heights and konus angles. The inner crown height were divided to 5mm, 6mm, and 7mm and konus angles was divided to $4^{\circ}$, $6^{\circ}$, and $8^{\circ}$. And then in each model, 5kg of $15^{\circ}$ mesial load was stressed on the central fossa of the 1st premolar and the 1st molar. The stresses and displacement were measured using the finite element analysis. The results were as follows 1. The maximum compressive strength was shown on the connective area of the abutment and the denture base. 2. As the angle of the inner crown becomes increased, the compressive strength was shown smaller. 3. As the height of the inner crown becomes increased, the maximum compressive strength was shown smaller while the compressive strength of the root apex and the residual ridge showed larger. 4. When the stress was loaded only on the 1st premolar, the more compressive strength was concentrated on the root apex area of the 1st premolar. 5. When the stress was loaded only on the 1st premolar, the compressive strength was concentrated uniformly on the abutment and the residual ridge. 6. When the stress was loaded only on the 1st molar, the maximum displacement was shown on the distal part of the residual ridge.

• 구강회복응용과학지
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• 제35권4호
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• pp.253-259
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• 2019

본 증례 보고는 하악 전치에 발생한 치은 퇴축을 laterally closed tunnel technique을 이용하여 처치하였다. 환자는 모두 과거 교정 치료 시 하악 전치의 경사도를 변화시켰으며 치주 적으로 얇은 생체형을 가지고 있었다. 터널링법으로 수여 부를 형성하고 구개 측에서 채득한 결합조직이식편을 터널내에 위치시킨 후 판막의 변연을 치근 중앙부를 향해 측방으로 이동시켜 봉합하여 이식편을 피개 하였다. 얇은 치주 생체형에도 불구하고 치근 피개를 성공적으로 얻을 수 있었으며 각화 치은의 획득과 주변 조직과의 색, 형태 조화로 심미성을 달성할 수 있었다.

• 한국자기공명학회논문지
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• 제24권1호
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• pp.1-8
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• 2020

An extracellular matrix protein, transforming growth factor-beta-induced protein (TGFBIp/βig-h3), which is induced by transforming growth factor-β in the human cornea, skin, and matrix of many connective tissues, is associated with the adhesion, migration, proliferation, and differentiation of various cells. TGFBIp contains four homologous repeat domains, known as FAS1 domains, where certain mutations have been considered to cause corneal dystrophies. In this study, backbone NMR assignments of FAS1-3/FAS1-4 tandem domain were obtained and compared with those previously known for the isolated FAS1-4 domain. The results corroborate in solution the inter-domain interaction between FAS1-3 and FAS1-4 in TGFBIp.

• 대한예방한의학회지
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• 제15권1호
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• pp.71-87
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• 2011

Objective : The present study is aimed at providing basic data to help oriental medical hospitals devise efficient operational plans by analyzing the medical expenses of in-patients in an oriental medical hospital and the factors affecting such expenses. Methods : PASW 18.0 was used to analyze the medical insurance program data of 929 patients who were discharged from a university oriental medical hospital(with 105 sick-beds) during the period from January 1 to December 31, 2010 after treatment under the coverage of health insurance and medical aid. Results : 1) Of all the patients hospitalized, 63.3% were females, their mean age was 52.73 years old, and 87.7% was covered by the health insurance program. The biggest number or 31.2% of the patients were treated by the department of acupuncture, 31.5% suffered mainly from the diseases of musculoskeletal system and connective tissues, and the average length of stay at the hospital was 19.49 days. 2) There were statistically significant differences in total medical expenses by age, clinical department in charge, principal diagnosis, and number of days hospitalized while daily average medical expenses differed depending on age, type of medical security, clinical department, principal diagnosis, and number of days staying at the hospital. 3) Total medical expenses were found significantly influenced by age, type of medical security, clinical department, principal diagnosis, and number of days hospitalized(explanatory power : 95.9%), whereas type of medical security, clinical department and principal diagnosis turned out to exercise significant influence on the daily average medical expenses(explanatory power : 26.9%). Conclusion : Oriental medical hospitals are suggested to make efforts to ensure geographical and economical accessibility for their main clients, the elderly and middle-aged, as well as to improve satisfaction of the clients with the medical service provided. They are also encouraged to work out systems to specialize in treatment with a focus on chronic degenerative and adult diseases. In addition, they are expected to try to enhance people's awareness of oriental medicine in an attempt to diversify the brackets of clients and increase frequency of their utilization.

• 한방안이비인후피부과학회지
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• 제28권3호
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• pp.101-113
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• 2015

Objectives : Microneedle therapy and Subcision are commonly used for therapeutic modalities of acne scars in Korean medicine. Beevenom could promote the expression of various cytokines, enzymes and receptors related to increasing collagen content of connective tissues. Through this mechanism, we considered that Beevenom pharmacopuncture therapy is applicable to the treatment of acne scars. We investigated twenty-seven patients with acne scars treated by Microneedle therapy and Subcision combined with Beevenom pharmacopuncture therapy and evaluated the clinical effect.Methods : Twenty-seven patients with acne scars were treated by Microneedle therapy and Subcision combined with Beevenom pharmacopuncture therapy and evaluated the clinical effect. for twelve weeks. The severity of acne scars and treatment effect were evaluate by standardized photography, GASC, ECCA scale and patient`s satisfaction. And side effects was also checked.Results : After twelve weeks for treatment, there was an improvement in GASC, ECCA scale. 77.8% of patient replied excellent or good on patient satisfaction survey. One patient sufferied from pruritus, papules and comedones as side effect of treatment.Conclusion : It can be considered that Microneedle, Subcision, Beevenom pharmacopuncture combining therapy are effective method for treating acne scars.

• Imaging Science in Dentistry
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• 제51권3호
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• pp.223-235
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• 2021

Purpose: This study aimed to summarize the impact of neck and head radiation treatment on maxillofacial structures detected on panoramic radiographs. Materials and Methods: In this systematic review, the authors searched PubMed Central, Embase, Scopus, Cochrane Central Register of Controlled Trials, Web of Science, and Google Scholar for original research studies up to February 2020 that included the following Medical Subject Headings keywords: words related to "radiotherapy" and synonyms combined with keywords related to "panoramic radiography" and "oral diagnosis" and synonyms. Only original studies in English that investigated the maxillofacial effects of radiotherapy via panoramic radiographs were included. The quality of the selected manuscripts was evaluated by assessing the risk of bias using Cochrane's ROBINS-I tool for non-randomized studies. Results: Thirty-three studies were eligible and included in this review. The main objectives pertained to the assessment of the effects of radiation on maxillofacial structures, including bone architecture alterations, periodontal space widening, teeth development abnormalities, osteoradionecrosis, and implant bone loss. The number of participants evaluated ranged from 8 to 176. Conclusion: The interaction between ionizing radiation and maxillofacial structures results in hazard to the tissues involved, particularly the bone tissue, periosteum, connective tissue of the mucosa, and endothelium. Hard tissue changes due to radiation therapy can be detected on panoramic radiographs.

• Biomolecules & Therapeutics
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• 제30권6호
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• pp.520-528
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• 2022

Mast cells are an effector cell that plays a pivotal role in type I hypersensitive immune responses. Mast cells exist in connective tissues, such as skin and mucosal tissue, and contain granules which contain bioactive substances such as histamine and heparin in cells. The granules of mast cells are secreted by antigen stimulation to cause the type I allergic hypersensitivity. In addition, stimulated by antigen, mast cells synthesize and secrete various eicosanoids and cytokines. While AT9283 is known to have anticancer effects, the therapeutic effect of AT9283 on allergic disorders is completely unknown. In this study, it was found that AT9283 reversibly inhibited antigen-IgE binding-induced degranulation in mast cells (IC₅₀, approx. 0.58 μM) and suppressed the secretion of the inflammatory cytokines IL-4 (IC₅₀, approx. 0.09 μM) and TNF-α (IC₅₀, approx. 0.19 μM). For a mechanism of mast cell inhibition, while not inhibiting Syk phosphorylation, AT9283 suppressed the activation of LAT, a downstream substrate protein of Syk, in a dose-dependent manner. As expected, AT9283 also inhibited the activation of PLCγ1 and Akt, downstream signaling molecules of Syk/LAT, and MAP kinases such as JNK, Erk1/2, and P38. In an in vitro protein tyrosine kinase assay, AT9283 directly inhibited Syk activity. Next, AT9283 dose-dependently inhibited passive cutaneous anaphylaxis (PCA), an IgE-mediated allergic acute response, in mice (ED50, approx. 34 mg/kg, p.o.). These findings suggest that AT9283 has potential to use as a new drug for alleviating the symptoms of IgE-mediated allergic disorders.

• Journal of Animal Science and Technology
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• 제65권2호
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• pp.311-323
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• 2023

Beef consumers valued meat quality traits such as texture, tenderness, juiciness, flavor, and meat color that determining consumers' purchasing decision. Most research on meat quality has focused on marbling, a key characteristic related to meat eating quality. However, other important traits such as meat texture, tenderness, and color have not much studied in cattle. Among these traits, meat tenderness and texture of cattle are among the most important factors affecting quality evaluation of consumers. Collagen is the main component of connective tissues.It greatly affects meat tenderness. The objective of this study was to determine significant variants and candidate genes associated with collagen contents trait (total collagen) through genome-wide association studies (GWAS). Phenotypic and genomic data from 135 Hanwoo were used. The BLUPF90 family program and GRAMMAR method for GWAS were applied in this study. A total of 73 potential single nucleotide polymorphisms (SNPs) showed significant associations with collagen content. They were located in or near 108 candidate genes. TMEM135 and ME3 genes were identified to have the most significant SNPs associated with collagen contents trait. Data indicated that these genes were related to collagen. Biological processes and pathways for the prediction of biological functions of candidate genes were confirmed. We found that candidate genes were involved in positive regulation of CREB transcription factor activity and actin cytoskeleton related to tenderness and texture of beef. Three genes (CRTC3, MYO1C and MYLK4) belonging to these biological functions were related to tenderness. These results provide a basis for improving genomic characteristics of Hanwoo for the production of tender beef. Furthermore, they could be used they could be used as an index to select desired traits for consumers.