• The Korean Journal of Pain
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• 제20권1호
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• pp.31-39
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• 2007

Background: Cervical radicular pain can arise fromvarious structures, including spinal nerves, discs, zygapophyseal joints, ligaments, and myofascial connective tissue. However, no adequate experiments have been found regarding methods for the microadhesiolysis of adhesional connective tissue around the zygapophyseal joints and nerves. The first objective of this study was to ascertain the effect of fluoroscopy guided interventional microadhesiolysis and nerve stimulation (FIMS) on chronic cervical radicular pain caused by zygapophyseal joint dysfunction. The second objective was to identify the duration of pain alleviation, as well as commonly occurring regions for zygapophyseal joint dysfunction. Methods: Twenty-eight patients were diagnosed with cervical radicular pain. The cervical zygapophyseal joints and adhesional structures around the cervical zygapophyseal joints were stimulated by adhesiolysis with a rounded needle; the procedure was performed once every second week. A visual analogue scale (VAS) for pain and neck range of motion (ROM) were used as indices for evaluating the degree of pain 1 and 3 months after completion of the procedures. A relief effect of FIMS was accepted when the VAS index decreased 50% compared with a previous VAS, and when there was absence of limitation of ROM. Results: Among the patients, 52% showed zygapophyseal joint dysfunction in C5-6, 38% in C4-5, 7% in C2-3, and 3% in C6-7. After performing FIMS, the VAS index decreased in most of the patients after 1 and 3 months (92.8% and 75%, respectively), and treatment frequency was $2.7{\pm}1.2$. There was no correlation between the number of FIMS procedures and the degree of VAS. Conclusions: FIMS is considered an effective modality in patients suffering from cervical radicular pain.

• Molecules and Cells
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• 제38권1호
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• pp.75-80
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• 2015

Osteoclasts are unique cells responsible for the resorption of bone matrix. MicroRNAs (miRNAs) are involved in the regulation of a wide range of physiological processes. Here, we examined the role of miR-26a in RANKL-induced osteoclastogenesis. The expression of miR-26a was upregulated by RANKL at the late stage of osteoclastogenesis. Ectopic expression of an miR-26a mimic in osteoclast precursor cells attenuated osteoclast formation, actin-ring formation, and bone resorption by suppressing the expression of connective tissue growth factor/CCN family 2 (CTGF/CCN2), which can promote osteoclast formation via upregulation of dendritic cell-specific transmembrane protein (DC-STAMP). On the other hand, overexpression of miR-26a inhibitor enhanced RANKL-induced osteoclast formation and function as well as CTGF expression. In addition, the inhibitory effect of miR-26a on osteoclast formation and function was prevented by treatment with recombinant CTGF. Collectively, our results suggest that miR-26a modulates osteoclast formation and function through the regulation of CTGF.

• 대한심미치과학회지
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• 제29권2호
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• pp.84-91
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• 2020

치주질환으로 손상된 치간유두의 재생은 아주 도전적인 과제이다. 지금까지 많은 치과의사들이 훌륭한 수술적인 방법을 고안하여 소개하였다. 지금까지 소개된 술식들을 순서대로 비교해보고 장단점을 비교해 치간유두를 재생을 위한 최적의 방법을 고안해보았다. 치주수술만으로 해결하기 어려운 좁은 치간유두 사이의 공간을 부분교정치료를 통하여 공간을 일시적으로 만들어 주는 것이 치은이식을 위해 큰 도움이 되었다. 치은이식은 마이크로 블레이드를 이용하여 박리를 시행하고 치은경계부에서 떨어진 수직절개를 하나만 시행해 여기를 통해 추가적인 박리와 이식편을 넣어서 치은이식을 시행하였다. 이렇게 교정치료를 동반하여 좁은 치간유두 사이의 폭을 벌려서 치은이식이 더 용이한 상태로 만들어 치은이식을 진행하고, 치은이 성숙한 뒤 교정력으로 다시 치아사이를 모아주는 술식(ELSA technique)을 통해 치간유두 재생을 할 수 있었다.

• 대한심미치과학회지
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• 제30권2호
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• pp.91-101
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• 2021

치간유두의 Connective Tissue Graft (CTG)는 좁은 공간 때문에 아주 까다롭다. 그래서 치간유두의 재생은 아주 도전적인 과제라고 생각한다. 이렇게 인접치와의 좁은공간에서 잇몸이식은 아주어렵지만, 3mm의 공간을 만들 수 있다면 CTG는 그렇게 어렵지 않다. 그래서 교정력을 이용하여 임시적으로 3mm의 공간을 만들어 주고 잇몸이식을 진행하였다. CTG는 마이크로블레이드를 이용하여 치은박리를 시행하였고, 치아사이의 공간에 수직절개를 만들어 그곳을 이용해서 결합조직을 이식하였다. 유지기간후에 교정력을 이용해 다시 치아사이의 공간을 모아서 치아를 배열하였다. 이 방법을 통해 치아사이의 치간유두를 재생하였고 이 술식을 이름을 ELSA (Enlargement of space - Labial graft - Squeezing - for Augmentation of papilla)로 이름지었다. 치주질환 등으로 치간유두가 소실된 경우, ELSA technique을은 치간유두를 재생하는 아주 효과적인 방법이다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제26권6호
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• pp.565-580
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• 2000

This study was designed to evaluate the influence of cultured epidermal tissue graft and the administration of transforming growth factor(TGF)-${\beta}_3$ on maxillary growth in surgically created palatal defects. A total of 155 rats were divided into 2 groups according to surgical timing : postnatal 2 weeks(n=95), 4 weeks(n=40) and control(unoperated) group(n=20). The postnatal 2-week surgical group was subdivided into 3 groups according to repair methods: conventional surgery(Von Langenbeck technique)group(n=23); cultured tissue graft group(n=25); and full thickness skin graft group(n=25). Additionally, recombinant human TGF-${\beta}_3$ was administered(30ng or 150ng) on collagen matrix in surgically created palatal defects during surgery(9 conventional surgeries, 9 cultured tissue grafts) in 2-week-old rats. The results showed that all types of surgical treatment decreased maxillary growth compared with the control(unoperated) group(p<0.0001). On the other hand, the tissue graft group, whether cultured tissue or grafted skin, contributed to increased maxillary growth(p<0.0001).And exogenous TGF-${\beta}_3$ might play a role in connective tissue proliferation and new bone generation during wound healing on palatal defects. Our results suggest that grafting cultured epidermis with collagen matrix decreases the scar tension on maxillary growth more than conventional palatal surgery does. Therefore, exogenous TGF-${\beta}_3$ may contribute to accelerate wound healing on palatal defects.

• Restorative Dentistry and Endodontics
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• 제41권1호
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• pp.44-54
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• 2016

Objectives: The purpose of this study was to evaluate the histopathological effects of an antioxidant therapy on the pulp tissue of rat teeth exposed to a bleaching gel with 35% hydrogen peroxide. Materials and Methods: Forty rats were subjected to oral ingestion by gavage of distilled water (DW) or ascorbic acid (AA) 90 min before the bleaching therapy. For the bleaching treatment, the agent was applied twice for 5 min each to buccal surfaces of the first right mandibular molars. Then, the animals were sacrificed at 6 hr, 24 hr, 3 day, or 7 day post-bleaching, and the teeth were processed for microscopic evaluation of the pulp tissue. Results: At 6 hr, the pulp tissue showed moderate inflammatory reactions in all teeth of both groups. In the DW and AA groups, 100% and 80% of teeth exhibited pulp tissue with significant necrosis and intense tissue disorganization, respectively. At 24 hr, the AA-treated group demonstrated a greater regenerative capability than the DW group, with less intense inflammatory reaction and new odontoblast layer formation in 60% of the teeth. For up to the 7 day period, the areas of pulpal necrosis were replaced by viable connective tissue, and the dentin was underlined by differentiated odontoblast-like cells in most teeth of both groups. Conclusions: A slight reduction in initial pulpal damage during post-bleaching was promoted by AA therapy. However, the pulp tissue of AA-treated animals featured faster regenerative potential over time.

• Journal of Korean Dental Science
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• 제16권2호
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• pp.172-181
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• 2023

Purpose: To investigate the effect of epidermal growth factor (EGF) with collagen matrix (CM) for increasing gingival thickness. Materials and Methods: In five mongrel dogs, bilateral gingival defects were surgically made on the maxillary canines. After two months, either a subepithelial connective tissue graft (group SCTG) or CM with EGF (0.1 ug/ml, group EGF) was grafted, and the flap was coronally positioned to cover the graft materials. The animals were sacrificed after three months. Intraoral scanning was performed for soft tissue analysis. Histologic and histomorphometric analyses were performed. Result: Two animals exhibited wound dehiscence during the healing phase, leaving three for analysis. No statistically significant difference was found in soft tissue changes (P>0.05). The level of gingival margin (GM) increased in both groups (1.02±0.74 mm in group SCTG vs. 1.24±0.83 mm in group EGF). Linear increases at the GM pre-augmentation in the soft tissue profile were 1.08±0.58 mm in group SCTG and 0.96±0.73 mm in group EGF. Histomorphometric parameters (keratinized tissue height, tissue thickness, and rete peg density) were not significantly different between the groups (P>0.05). Conclusion: EGF loaded onto CM led to comparable gingival phenotype enhancement to SCTG.

• 대한수의학회지
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• 제37권2호
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• pp.245-252
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• 1997

To investigate morphological changes in the exocrine pancreas of chicken after pancreatic duct ligation, experimental animals were subdivided to control, 12 hours, 1 day, 2 days, 4 days, 7 days and 10 days groupes and all of three pancreatic ducts of chicken were ligated by surgical procedure and then the morphological changes were observed. In pancreatic ducts, once for a while the ducts were dilated on 12 hours after pancreatic duct ligation and then they were obstructed because of proliferated epithelial cells and connective tissues in pancreatic duct. Marginal dissociation of acini was detected in 12 hours after pancreatic duct ligation and then dissociation of acini was increased with time and finally in 4 days after pancreatic duct ligation the acini showed completely dissociation except periductular regions and around pancreatic islets. Most of dissociated acini cells showed marginal condensation of nuclear chromatin and atropy of cytoplasm, namely, apoptotic features were detected in dissociated acinar cells. Interacinar spaces of dissociated acinar regions were dilated and fulfilled with increased connective tissue and in 4 days after pancreatic duct ligation, deposition of lymphocytes and hemocytes was occurred.

• Journal of Mechanical Science and Technology
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• 제17권8호
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• pp.1164-1170
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• 2003

The objectives of this work here focus on the differences in responses to multiple cyclic tests of different sections along the length of the same tendon. Tendon specimens were obtained from the hindlimbs of canines and frozen to -70$^{\circ}C$. After thawing, specimens were mounted in the immersion bath at room temperature (22$^{\circ}C$) , preloaded to 0.13 N and then subjected to 3% or 4% of the initial length at a strain rate of 5%/sec. It was found that different sections of the same long tendons had different resistances to deformation. In general, the bone end sections were stiffer and carried greater loads for a given strain than the muscle end sections, and the mid-portions were the least stiff and carried the smallest loads for a given strain. The results of this study offer new information about the mechanical responses of collagenous tissues. We know more about their responses to multiple cyclic extensions and how their responses are different from the positions along the length of the tendon specimen. The nature and causes of these differences in the stiffness are not fully known. However, it is clear that differences in the mechanical response of tendons and other connective tissues are significant to musculoskeletal performance.

• 한국임상수의학회지
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• 제36권4호
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• pp.233-237
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• 2019

A 10-year-old castrated male Shih Tzu dog was submitted to a local animal hospital with a mass from gingiva to maxillofacial skeleton. Computed tomography revealed that strong invasion of the mass result in osteolysis in orbit and frontal bone. The excised mass was presented to the Pathology Department of the Veterinary Medicine, Jeju National University. Surgically excised mass was rubbery to firm in consistency. Histologically, the neoplastic mass was composed of irregular or interdigitating cords, islands or pseudo-glandular structures of stratified epithelial cells. These cords or islands showed typical palisading pattern of neoplastic epithelial cells to periphery without intercellular bridge (desmosome) and surrounded by eosinophilic immature collagenous matrix. Some area showed islands of well differentiated keratinizing squamous cell foci. Some lumen of glandular structures contained fibrin-like materials and RBC. These neoplastic cells showed marked invasive tendency to adjacent connective tissues and bony tissues, therefore solitary neoplastic cells were widely distributed throughout the surround connective tissue. The neoplastic cells showed positive reactions for pan-CK and CK14, weakly positive reaction for CK5/6. And the surrounding immature collagenous matrix was only labeled for vimentin.