• The Korean Journal of Mycology
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• v.44 no.4
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• pp.338-341
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• 2016

A dark septate endophytic fungal strain 13E043 was isolated from the needle leaves of Thuja koraiensis from a forest in Korea. Morphological characteristics of conidia and phialids. Along with a molecular phylogenetic analysis based on the internal transcribed spacer region of rDNA, the isolate was identified as Phialocephala piceae (also known as Phaeomollisia piceae). This is the first report of a dark septate endophyte isolated from the foliage of conifer trees in Korea.

• Asian Journal of Turfgrass Science
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• v.9 no.4
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• pp.317-329
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• 1995

The study of standing crop of the ground vegetation under the coniferous stands was pursuited in regard of soil nutrients at Kwangnung plantation from May to Octoher, 1971 and 1991. The coniferous stands implied pine and larch associations of which the young stands of each as-sociation were 9~12 years and the older one 40~70 years. The larch stands were more plentiful in species than in the pine stands and the Festuca ovina and Oplismenus burmannii each were dominant species of pine and larch stands. The standing crop of pine stands is from 98.02g /$m^2$ to 385.32g /$m^2$ The standing crops of those stands showed great increase from June to August when the air temperature and rainfall were fairly high. The standing crop has correlation to total nitrogen and organic matter in the soil.

• The Korean Journal of Mycology
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• v.48 no.2
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• pp.117-124
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• 2020

In this study, we isolated endophytic fungi from the conifer leaves of Pinus densiflora inhabiting Mt. Gyehwasan in Korea. We identified the isolated fungal strains based on their morphological characteristics and phylogenetic analysis using DNA sequences of the internal transcribed spacer, small subunit rDNA, large subunit rDNA, and β-tubulin region. In the process of this study, we confirmed two endophytic fungi that have not been recorded previously in Korea, Lophiostoma macrostomum and Pestalotiopsis lespedezae. In this report, we described the morphological characteristics of these fungal strains and the results of their molecular analysis.

• The Korean Journal of Mycology
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• v.47 no.1
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• pp.35-42
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• 2019

We isolated endophytic fungi from the conifer leaves of Pinus densiflora inhabiting Buan-gun, Jeollabuk-do, Korea. We identified the isolated fungal strains based on phylogenetic analysis performed using the nucleotide sequences of the internal transcribed spacer, large subunit, and beta-tubulin. We confirmed the presence of three novel endophytic fungi in Korea, namely Paracamarosporium hawaiiense, Tubakia dryina, and Zasmidium fructigenum. In this report, we described the morphological characteristics of these fungal strains and the results of their phylogenetic analysis.

• The Korean Journal of Mycology
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• v.50 no.3
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• pp.173-181
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• 2022

Endophytic fungi were isolated from needle leaves of two conifer species in Korea. The fungal isolates were identified based on their morphological characteristics and phylogenetic analyses of their internal transcribed spacer and large-subunit rDNA regions. Three species of endophytic fungi, namely Celosporium laricicola, Neocatenulostroma germanicum, and Phaeophleospora eucalypticola were the first records in Korea. In this study, we reported the morphological and phylogenetic characteristics of these fungi.

• Journal of Korean Society of Forest Science
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• v.98 no.4
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• pp.464-471
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• 2009

Vegetation types of the native conifer forests in the ridge of the Baekdudaegan, South Korea, were classified using the Braun-Blanquet method and TWINSPAN and its ecological characteristics analyzed with special repect to floristic composition. The vegetation type was classified into two types (Pinus densiflora-Carex humilis and Pinus koraiensis-Dryopteris crassirhizoma types) and seven groups. Mean species richness and total plot cover per unit area ($/100m^2$) were $21.9{\pm}8.9$ spp. and $146.9{\pm}32.3$%, respectively. The plant species composed of 82 families, 217 genera, and 387 species and the three major families, Compositae, Liliaceae, and Rosaceae, made up about 25.6% of the total species. The 7.8% of total families were the families with occurrence of only one species. The highest relative importance value was 19.2, the species belonged to Pinaceae and the pattern was different from species richness pattern of other families.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.24-24
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• 2018

Degraded plantation forests are increasing because of unfavorable forestry conditions prevailing in Japan, including falling timber prices, increasing operational costs, and aging and declining forestry workforce. To remedy this situation, appropriate management strategy is required. This study introduces the challenges of Odai Town, Mie Prefecture that employed a new management strategy by evaluating site suitability for conifer forests and that proposes a new forest management regime of planting deciduous trees in unsuitable sites. The site suitability for conifer forests was evaluated from two aspects: the natural site conditions and the relationship among site conditions, growth, and damage by Anaglyptus subfasciatus Pic. in Cryptomeria japonica D. Don and Chamaecyparis obtusa Sieb. Et Zucc. forests. By analyzing the relationship among site conditions, growth, and insect damage based on field data obtained in plantation forests, growth evaluation and insect damage evaluation maps were developed. Based on the natural forest investigation, natural site condition maps for C. japonica and C. obtusa were established. Furthermore, by integrating these evaluation maps with the forest road maps showing the accessibility to the forest, the forest management regime for the whole plantation area of Odai Town was established. The forest management regime map indicates the sites suitable for forestry: suitable for long-rotation, short-rotation, and potential sites for short-rotation. The sites unsuitable for forestry were considered to be more suitable for broadleaved forests. Clear-cutting was conducted in a small area and different seral stage saplings (approximately 20 deciduous tree species) suitable to the site conditions were planted in an area of $80-120m^2$ protected by deer-fences. This might establish a forest composed of many species with a multilayer vertical forest structure in a short period. The planted saplings were distributed neither randomly nor uniformly to reflect the natural distribution of trees in the forest. A challenge to develop new products using the deciduous trees has started, such as wood chips for preparing smoked food, essential oil, and deodorizer. As these challenges have just begun, their effects on enhancing sustainable resource management are still being monitored. Even with the challenges, this regime can be of high value as a management strategy to remedy the situation of expansion of degraded forests in Japan.

• The Korean Journal of Quaternary Research
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• v.15 no.2
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• pp.75-81
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• 2001

We have analyzed the pollen sequence since the Late Glacial at Sorori Archaeological Site. The Sorori Site is an open site located at Soro-ri, Oksan-myeon, Cheongwon-gun, Chungchongbukdo. The main results are as follows: Four forest zones have been distinguished. \circled1 OS-1 zone : Pinus-Abies-Picea forest (the sub-alpine conifer forest), the Late Glacial (about 15,000~10,000yr. B.P.) ; \circled2 OS-2 zone : Pinus-Colyus forest (the mixed conifer and deciduous broad-leaved forest), R I (about 10,000 yr.B.P.) : \circled3 OS-3 zone : Alnus-Quercus forest (the cool temperate deciduous broadleaved forest), R II(about 10,000~2,000yr.B.P.) : \circled4 OS-4 zone : Pinus forest (the conifer forest), R III (about after 2,000yr.B.P.)

• Parasites, Hosts and Diseases
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• v.51 no.3
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• pp.319-325
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• 2013

This study describes the seasonal distribution of larvae, nymph, and adult life stages for 3 species of ixodid ticks collected by tick drag and sweep methods from various habitats in the Republic of Korea (ROK). Grasses less than 0.5 m in height, including herbaceous and crawling vegetation, and deciduous, conifer, and mixed forests with abundant leaf/needle litter were surveyed at United States (US) and ROK operated military training sites and privately owned lands near the demilitarized zone from April-October, 2004 and 2005. Haemaphysalis longicornis Neumann adults and nymphs were more frequently collected from April-August, while those of Haemaphysalis flava Neumann and Ixodes nipponensis Kitaoka and Saito were collected more frequently from April-July and again during October. H. longicornis was the most frequently collected tick in grass habitats (98.9%), while H. flava was more frequently collected in deciduous (60.2%) and conifer (57.4%) forest habitats. While more H. flava (54.1%) were collected in mixed forest habitats than H. longicornis (35.2%), the differences were not significant. I. nipponensis was more frequently collected from conifer (mean 8.8) compared to deciduous (3.2) and mixed (2.4) forests.

• Korean Journal of Plant Resources
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• v.27 no.3
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• pp.242-248
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• 2014

This study was conducted to establish an efficient transformation system by using somatic embryogenesis in an important Korean native conifer, Korean fir (Abies koreana). Embryogenic masses were induced from mature zygotic embryos of the Korean fir on Schenk and Hildebrandt medium, which was supplemented with thidiazuron. For genetic transformation, the embryogenic masses were co-cultivated with a disarmed Agrobacterium tumefaciens strain C58/pMP90 containing the plasmid vector pBIV10 or LBA4404 containing the plasmid vector MP90. Both vectors contain the kanamycin resistance and beta-glucuronidase (GUS) reporter genes. A total of 48 lines of embryogenic masses were selected on mLV medium containing $50{\mu}g/mL$ of kanamycin after 4 weeks of culture, following 3 days of co-cultivation with A. tumefaciens strain C58/pMP90 carrying pBIV10 (none of the lines was cultivated with strain LBA4404 carrying MP90). Quantitative real-time PCR was performed, and high levels of GUS transcripts were observed in the 48 putative transgenic lines; however, the control (non-transgenic line) showed negative results. Results of histochemical staining showed that the expression of the GUS reporter gene was observed in somatic embryos that developed from the embryogenic masses of all 48 lines. Stably transformed cultures were successfully produced by co-cultivation with A. tumefaciens strain C58/pMP90 carrying pBIV10 in Korean fir. Here, we have reported an Agrobacterium-mediated gene transfer protocol via somatic embryogenesis that may be helpful in developing breeding and conservation strategies for the Korean fir.