Browse > Article
http://dx.doi.org/10.4489/KJM.20220018

Characterization of Three Species of Endophytic Fungi Isolated from Conifer Leaves in Korea  

Jae-Wook Choi (Department of Biology Education, Korea National University of Education)
Ju-Hui Gwon (Department of Biology Education, Korea National University of Education)
Jong-Chul Lee (Department of Biology Education, Korea National University of Education)
Hyeok Park (Department of Biology Education, Korea National University of Education)
Ahn-Heum Eom (Department of Biology Education, Korea National University of Education)
Publication Information
The Korean Journal of Mycology / v.50, no.3, 2022 , pp. 173-181 More about this Journal
Abstract
Endophytic fungi were isolated from needle leaves of two conifer species in Korea. The fungal isolates were identified based on their morphological characteristics and phylogenetic analyses of their internal transcribed spacer and large-subunit rDNA regions. Three species of endophytic fungi, namely Celosporium laricicola, Neocatenulostroma germanicum, and Phaeophleospora eucalypticola were the first records in Korea. In this study, we reported the morphological and phylogenetic characteristics of these fungi.
Keywords
Conifers; Fungal endophytes; Needle leaves;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Crous P, Schoch C, Hyde K, Wood A, Gueidan C, De Hoog G, Groenewald J. Phylogenetic lineages in the Capnodiales. Stud Mycol 2009;64:17-47.    DOI
2 Markovskaja S, Kacergius A, Davydenko K, Fraser S. First record of Neocatenulostroma germanicum on pines in Lithuania and Ukraine and its co-occurrence with Dothistroma spp. and other pathogens. For Pathol 2016;46:522-33.    DOI
3 Crous PW, Wingfield MJ, Richardson D, Leroux J, Strasberg D, Edwards J, Roets F, Hubka V, Taylor P, Heykoop M. Fungal Planet description sheets: 400-468. Persoonia 2016;36:316-458.   DOI
4 Carroll G. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 1988;69:2-9.    DOI
5 Sinclair J, Cerkauskas R. Endophytic fungi in grasses and woody plants systematics, Ecology and Evolution. Minnesota: APS Press; 1996. 
6 Serfling A, Wirsel SG, Lind V, Deising HB. Performance of the biocontrol fungus Piriformospora indica on wheat under greenhouse and field conditions. Phytopathology 2007;97:523-31.    DOI
7 Khiralla A, Mohamed I, Thomas J, Mignard B, Spina R, Yagi S, Laurain-Mattar D. A pilot study of antioxidant potential of endophytic fungi from some Sudanese medicinal plants. Asian Pac J Trop Med 2015;8:701-4.    DOI
8 Ghaffari MR, Ghabooli M, Khatabi B, Hajirezaei MR, Schweizer P, Salekdeh GH. Metabolic and transcriptional response of central metabolism affected by root endophytic fungus Piriformospora indica under salinity in barley. Plant Mol Biol 2016;90:699-717.    DOI
9 Rodriguez R, White Jr J, Arnold A, Redman R. Fungal endophytes: diversity and functional roles. New Phytol 2009;182:314-30.    DOI
10 Douanla-Meli C, Langer E. Diversity and molecular phylogeny of fungal endophytes associated with Diospyros crassiflora. Mycology 2012;3:175-87. 
11 Nascimento T, Oki Y, Lima D, Almeida-Cortez J, Fernandes GW, Souza-Motta C. Biodiversity of endophytic fungi in different leaf ages of Calotropis procera and their antimicrobial activity. Fungal Ecol 2015;14:79-86.    DOI
12 Rodriguez R, White Jr J, Arnold A, Redman R. Fungal endophytes: diversity and functional roles. New Phytol 2009;182:314-30.    DOI
13 Malhadas C, Malheiro R, Pereira JA, de Pinho PG, Baptista P. Antimicrobial activity of endophytic fungi from olive tree leaves. World J Microb Biot 2017;33:1-12.    DOI
14 Gange AC, Eschen R, Wearn JA, Thawer A, Sutton BC. Differential effects of foliar endophytic fungi on insect herbivores attacking a herbaceous plant. Oecologia 2012;168:1023-31.    DOI
15 National Institue of Biological Resources. National list of species of Korea. Incheon, Korea: National Institue of Biological Resources; 2022 [cited 2022 Aug]. Available from: https://kbr.go.kr. 
16 Kong WS. Species composition and distribution of native Korean conifers. J Korean Geogr Soc 2004;39:528-43. 
17 Luo X, Yu C. First report of damping-off disease caused by Fusarium oxysporum in Pinus massoniana in China. J Plant Dis Protect 2020;127:401-9.    DOI
18 Kim ES, Lee JS, Park GE, Lim JH. Change of subalpine coniferous forest area over the last 20 years. J Korean Soc For Sci 2019;108:10-20. 
19 Ravnikar M, Tercelj M, Janes D, Strukelj B, Kreft S. Antibacterial activity of endophytic fungi isolated from conifer needles. Afr J Biotechnol 2015;14:867-71.    DOI
20 Tanney JB, McMullin DR, Green BD, Miller JD, Seifert KA. Production of antifungal and antiinsectan metabolites by the Picea endophyte Diaporthe maritima sp. nov. Fungal Biol 2016;120:1448-57.    DOI
21 Ismail HM, Hussain A, Khan SA, Iqbal A, Lee I-J. An endophytic fungus Aspergillus violaceofuscus can be used as heat stress adaptive tool for Glycine max L. and Helianthus annuus L. J Appl Bot Food Qual 2020;93:112. 
22 Eo JK, Eom AH. Diversity of foliar endophytic fungi inhabiting coniferous trees in Korea. Kor J Mycol 2018;46:205-11. 
23 White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 1990;18:315-22. 
24 Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 1990;172:4238-46.    DOI
25 Alves A, Crous PW, Correia A, Phillips A. Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Divers 2008;28:1-13. 
26 Tamura K, Stecher G, Kumar S. MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 2021;38:3022-7.    DOI
27 Tsuneda A, Davey M, Tsuneda I, Currah R. Two new dothideomycetous endoconidial genera from declining larch. Botany 2010;88:471-87.