• Title/Summary/Keyword: confocal microscope

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Statistical Analysis of Fluorescence Correlation Spectroscopy of Ultra Low Concentration Molecules with a Confocal Microscope

  • Lee, Soon-Hyouk;Lim, Gyu-Chang;Kim, Soo-Yong;Kim, Eun-Kyung;Kim, Hak-Sung;Kim, Sok-Won
    • Journal of the Optical Society of Korea
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    • v.12 no.3
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    • pp.170-173
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    • 2008
  • In this study, we simulated a statistical model of FCS(fluorescence correlation spectroscopy) based on a Poisson process to understand and explain observations of the experiment performed on molecules of ultra-low concentration by the home-built laser-scanning confocal microscope. The statistical model confirmed that the relative mean square amplitude of fluctuations is shown to be inversely proportional to the average number of molecules, even in the ultra-low concentration, if some conditions are satisfied. Signal-to-noise ratio and the variability of dwelling time under the confocal volume were found to be effective conditions for the experiment.

Characterization of Fracture Roughness in Coarse.medium.fine Grained Granite (암반 불연속면의 거칠기 특성 - 조.중.세립질 화강암을 중심으로 -)

  • 김종태;정교철;김만일;송재용;박창근
    • The Journal of Engineering Geology
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    • v.14 no.2
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    • pp.147-168
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    • 2004
  • Purpose of this study is to quantitatively characterize the fracture roughness which was measured with a confocal laser scanning microscope. The roughness discrete data measured by confocal laser microscope were analyzed by spectral analysis and fast Fourier transform (FFT).The roughness data by used noise reduction filter were applied for fractal analysis to describe roughness features quantitatively. Artificial fractures created by Brazilian test on granites were used to measure fracture roughness under the confocal laser scanning microscope. Measurements were performed along three scan lines on each fracture surface. 36 scan lines were determined on 12 specimens in total. Features of roughness showed that coarse and medium grained granites tend to more rough features than those of fine grained granites. Continuous analog data of roughness is possible to described as discrete data of measure roughness with a fixed interval under the confocal laser microscope. Results of FFT with the measured data showed the highest values on the second harmonics. Distribution of average amplitude of second harmonics was observed 0.9853 in coarse grained granite, 1.0792 in medium grained granite and 0.6794 in fine grained granite. This indicates that the larger roughness has the higher energy of harmonics as the result of fractal analysis in low frequency zone.

New Measurement of Whitening Effects by Using Confocal Scanning Laser Microscope (CSLM) (Confocal Scanning Laser Microscope (CSLM)을 이용한 신규 미백 효과 측정 연구)

  • Kim, Myong Ki;Cho, Seok-Cheol;Nam, Gaewon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.3
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    • pp.279-285
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    • 2015
  • Hyperpigmentation on face is a highly anxiety-producing symptom, especially for women from the aspect of beauty. Pigmentation of the skin is related to the amount of melanin that provides protection against UV radiation. In vivo reflectance confocal microscopy is a non-invasive imaging tool allowing visualization of the skin without tissue alteration, by placing a microscopy directly on the living skin. The aim of this study was to develop the new evaluation method of whitening effects using in vivo reflectance confocal microscopy and to validate other instruments for measuring skin colors, and UV-induced hyperpigmentation was elicited on the inside skin of the forearm. It suggested that the new method for whitening effects using the confocal microscopy was useful to evaluate the de-pigmentation products and to easy for understanding to customers.

Fabrication and studies on the properties of a spinning-disk confocal microscope (회전원판식 공초점 현미경의 구성과 광학특성)

  • 신은성;남기봉
    • Korean Journal of Optics and Photonics
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    • v.8 no.4
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    • pp.255-259
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    • 1997
  • In this paper, the result of a performance study on a home-built spinning disk type confocal microscope is presented. The confocal microscope was fabricated with a Nipkow disk made of the made of the microfilm. The throughput of the disk was 0.5%, allowing the observation of specimen with higher reflectivities only. A laser diode at 692.7nm was used as the light source. The topographic structures of a PC ROM and the CD ROM were observed with sufficient reliability, while the effect of the convolution of the beam size with the finite object size was found dominant. Also the shadowing effect by the etched pattern was observed.

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Scanning confocal microscope of a surface following effect using a quad-detector (4분할 광검출기를 이용한 표면 추적 효과의 공초점 현미경)

  • 김수철;송장섭;권남익
    • Korean Journal of Optics and Photonics
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    • v.9 no.1
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    • pp.1-4
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    • 1998
  • A compact scanning confocal microscope has been constructed using a quad-detector in a CD-player. The variation of error signal on depth which obtained by a quad-detector is used in the scanning confocal microscope. Bipolar error signals, which is sensitive near a focus, give a surface following effect whiout following the surface. In the case of small depth difference, the difference of materials through the reflection signal has been identified. The image which uses reflection signal only, has been obtained with the same setup. And, the results obtained in two different way were compared and analyzed.

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A New Method for Measuring Refractive Index with a Laser Frequency-shifted Feedback Confocal Microscope

  • Zhou, Borui;Wang, Zihan;Shen, Xueju
    • Current Optics and Photonics
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    • v.4 no.1
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    • pp.44-49
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    • 2020
  • In this paper, a new method is presented to measure the refractive index of single plain glass or multilayered materials, based on a laser frequency-shifted confocal feedback microscope. Combining the laser frequency-shifted feedback technique and the confocal effect, the method can attain high axial-positioning accuracy, stability and sensitivity. Measurements of different samples are given, including N-BK7 glass, Silica plain glass, and a microfluidic chip with four layers. The results for N-BK7 glass and Silica plain glass show that the measurement uncertainty in the refractive index is better than 0.001. Meanwhile, the feasibility of this method for multilayered materials is tested. Compared to conventional methods, this system is more compact and has less difficulty in sample processing, and thus is promising for applications in the area of refractive-index measurement.