• ALGAE
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• v.25 no.1
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• pp.11-15
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• 2010

Two new genera of red algae, Madagascaria erythrocladioides West et Zuccarello and Pseudoerythrocladia kornmannii West et Kikuchi (Erythropeltidales, Compsopogonophyceae, Rhodophyta), were previously described using molecular analysis and confocal microscopy of isolates in laboratory culture. We examined the ultrastructure of both genera to compare with ultrastructure of other members of the class Compsopogonophyceae. Both genera had Golgi bodies not associated with mitochondria and chloroplasts with a peripheral encircling thylakoid similar to all other members of the class studied thus far. Confocal autofluorescence images showed that Madagascaria has a single round central pyrenoid while Pseudoerythrocladia has no pyrenoid. Our electron microscopic work confirms these initial observations. Tables and keys are presented that assist in interpreting cellular details of genera in the class Compsopogonophyceae.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.4
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• pp.295-297
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• 2006

Potassium (K) distribution in rice (Oryza sativa L.) root was studied by confocal laser microscopy, using potassium sensitive fluorescent dye potassium-binding benzofuran isophthalate (PBFI). Significantly high intensity of K-specific fluorescence was detected at the root cap region followed by meristematic and basal regions. A negligible or fainted fluorescence was observed at the root hairs area. These results suggest that K is heavily distributed in the apical area of rice root, which may be required in higher concentration for division and extension of cells, as it is the rapidly growing region of the root, moreover, may also be involved in water uptake by creating osmotic gradient across membranes.

• Journal of the Optical Society of Korea
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• v.9 no.2
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• pp.49-53
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• 2005

We report a new configuration of a reflection-type confocal scanning optical microscope system for measuring the refractive index profile of an optical waveguide. Several improvements on the earlier design are proposed; a light emitting diode (LED) at 650 nm wavelength instead of a laser diode (LD) or He-Ne laser is used as a light source for better index precision, and a simple longitudinal linear scanning and curve fitting techniques are adapted instead of a servo control for maintaining an optical confocal arrangement. We have obtained spatial resolution of 700 nm and an index precision of $2\times10^{-4}$. To verify the system's capability, the refractive index profiles of a conventional multimode fiber and a home-made four-mode fiber were examined with our proposed measurement method.

• Journal of the Optical Society of Korea
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• v.9 no.1
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• pp.22-25
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• 2005

The differential heterodyne interferometer (DHI) is suitable for precise measurement of step height and line width, since its differential configuration can significantly reduce disturbances from the environment [1,2]. Like most phase measuring interferometers, however, the DHI is limited, in that it can obtain only the phase from 0 to 2π, because of the sinusoidal nature of the optical interference involved. Thus, the measurable step height is limited to one quarter of the wavelength of the light source. This study describes a confocal differential heterodyne interferometer (CDHI) for measuring step heights of several micrometers, with a high resolution and line width with high repeatability. The CDHI has a simple structure and rapid measurement speed.

• Korean Journal of Optics and Photonics
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• v.17 no.2
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• pp.186-190
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• 2006

The use of null optics is proposed as a new concept for the precise alignment of a confocal unstable resonator. The characteristics of the proposed null optics are investigated and analysed with the designed null lens for a real confocal unstable resonator of which the length is 3.5 m. As a result of the analysis, the pupil map data are shown about the despace error of 1.0 mm and tilt error of 1.0 mrad.

• Korean Journal of Optics and Photonics
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• v.10 no.3
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• pp.221-225
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• 1999

The positive branch confocal unstable resonator equipped with an intra-cavity beam splitter has been studied experimentally to achieve low beam divergence in the copper vapor laser. Output laser beams of 0.15 mrad divergence was achieved from the laser system with a 10 cm curvature convex mirror (M=30), and laser beams of 0.1 mrad was achieved for a 5 cm curvature convex mirror (M=60). The power density of the far-field beam from the M=60 resonator laser was 130 times higher than the case of the 2 mrad-operation with the plane-parallel resonator. We also observed the self-mode-locking from the unstable resonator laser pulses.

• Journal of Korea Multimedia Society
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• v.24 no.11
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• pp.1472-1480
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• 2021

Image stacking technique is one of the key techniques for complex surface reconstruction. The process includes sample collection, image processing, algorithm editing, surface reconstruction, and finally reaching reliable conclusions. Since this experiment is based on laser scanning confocal microscope to collect the original contour information of the sample, it is necessary to briefly introduce the relevant principle and operation method of laser scanning confocal microscope. After that, the original image is collected and processed, and the data is expanded by interpolation method. Meanwhile, several methods of surface reconstruction are listed. After comparing the advantages and disadvantages of each method, one-dimensional interpolation and volume rendering are finally used to reconstruct the 3D model. The experimental results show that the final 3d surface modeling is more consistent with the appearance information of the original samples. At the same time, the algorithm is simple and easy to understand, strong operability, and can meet the requirements of surface reconstruction of different types of samples.

• Current Optics and Photonics
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• v.5 no.6
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• pp.660-671
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• 2021

We present a novel optical system for an augmented reality head-up display (AR HUD) with two virtual images at different conjugates by employing a confocal off-axis two-mirror and introducing the horopter circle. For a far virtual image with large asymmetrical aberrations, we initially obtain an off-axis two-mirror system corrected for these aberrations and compensated for the down angle by configuring its parameters to satisfy the confocal and Scheimpflug conditions, respectively. In addition, this system is designed to reduce the biocular parallax by matching Petzval surface into the longitudinal horopter circle in a near virtual image. This design approach enables us to easily balance the residual aberrations and biocular parallax when configuring the optical system with two different conjugates, which results in an AR HUD available for near and far virtual images together.

• Journal of Advanced Information Technology and Convergence
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• v.10 no.1
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• pp.25-36
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• 2020

Motivation: Fibers as the extracellular filamentous structures determine the shape of the cytoskeletal structures. Their characterization and reconstruction from a 3D cellular image represent very useful quantitative information at the cellular level. In this paper, we presented a novel automatic method to extract fiber diameter distribution through a pipeline to reconstruct fibers from 3D fluorescence confocal images. The pipeline is composed of four steps: segmentation, skeletonization, template fitting and fiber tracking. Segmentation of fiber is achieved by defining an energy based on tensor voting framework. After skeletonizing segmented fibers, we fit a template for each seed point. Then, the fiber tracking step reconstructs fibers by finding the best match of the next fiber segment from the previous template. Thus, we define a fiber as a set of templates, based on which we calculate a diameter distribution of fibers.

• 제어로봇시스템학회:학술대회논문집
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• 2001.10a
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• pp.161.6-161
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• 2001

Confocal scanning microscopy (CSM) has an important role as the three-dimensional profiler. An image distribution can be reconstructed by a correlation analysis of spots with the bandwidth of radio frequency. But it is a serious problem for the high performance to align the optical components. Especially, the parasitic motion of focus on the detector gives rise to the fatal distortion of an image profile named the extinction effect while using acousto-optical(AO) deflector. An image profile can be regenerated in CSM with many advantages of non-contact, high speed and high resolution comparatively. In addition to the axial response of the primary focus, the lateral movement of it gives a necessity of the unitary lens to the scanning system. While using the beam deflector, the pupil of beam may be fixed at the nominal position. Furthermore, the use of a deflector may result in ...