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Growth and Survival Rates of Flat Oyster, Ostrea denselamellosa, by Condition of Larval Cultivation (벗굴 (Ostrea denselamellosa) 유생의 생육조건에 따른 성장과 생존)

  • Yang, Moon-Ho;Oh, Bong-Se;Han, Chang-Hee
    • The Korean Journal of Malacology
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    • v.19 no.2
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    • pp.133-142
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    • 2003
  • For the effective seedling production of flat oyster, Ostrea denselamellosa, dietary value of live food, densities, water temperature and salinity on growth and survival rate of the larvae were examined. In rearing larvae by feeding them phytoplankton diets, the optimal survival rate and growth rate of larvae were found using a mixed phytoplankton diet which was mixed with Isochrysis galbana, Chaetoceros calcitrans and Chlorella sp. The highest growth and survival rates of the larvae were 208.4% and 38.8% with the phytoplankton diet. In growth and survival rates of larvae with various rearing densities, the highest survival and growth rates were 228.1% and 29.0% at the density of 2 individuals/ml. In observing rearing experiments of the flat oyster larvae under various temperature conditions, average growth rates of the larvae in respect to shell length were 202.2%, 240.4%, 250.6% and 121.3% in natural water temperatures (18-22$^{\circ}C$), 24$^{\circ}C$, 28$^{\circ}C$ and 32 $^{\circ}C$, respectively. And average survival rates of the larvae were 16.0%, 32.0%, 13.0% and 0% in natural water temperatures (18-22$^{\circ}C$), 24$^{\circ}C$, 28$^{\circ}C$ and 32$^{\circ}C$, respectively. In rearing at various salinities, the highest growth rates of the larvae in shell length was 240.0% at 30.0 psu and the highest survival rate was 31.0% at 25 psu.

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Petrography and Geochemistry of the Ultramafic Rocks from the Hongseong and Kwangcheon areas, Chungcheongnam-Do. (충남 홍성 및 광천 지역 초염기성암의 암석 및 지구화학)

  • Song Suckhwan;Choi Seon Gyu;Oh Chang Hwan;Seo Ji Eun;Choi Seongho
    • Economic and Environmental Geology
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    • v.37 no.5
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    • pp.477-497
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    • 2004
  • In the Hongseong and Kwangcheon areas, two ultramafic rocks are exposed as isolated bodies in the Precambrian Kyeonggi gneiss complex. The ultramafic rocks extend for several hundred meters to NNE direction and are contact with adjacent metasediments by steeply dipping faults. The rocks are dunite or harzburgite showing dominantly equigranular-mosaic and protogranular textures with a minor amount of porphyroclastic textures. They contain varying amounts of fosteritic olivine (F$o_{0.91-0.93}$), magnesian pyroxene (E$n_{0.89-0.93}$) and tremolitic to magnesian hornblende with minor amounts of spinel, serpentine, chlorite, magnetite, phlogopite and talc. The rocks are in contrast with adjacent gneiss complex or metabasite (amphibole, biotite, plagioclase, alkali-feldspar and quartz). Geochemically, these ultramafic rocks are characterized by high magnesium number (M$g_#$> 0.88) and transitional element (mainly, Ni>1716 ppm, Cr>1789 ppm), low alkali element (e.g. $K_2$O<0.09 wt.%, Na$_2$O<0.19 wt.%) and depletion of incompatible elements. The calculated correlation coefficients showed good positive correlations among the ferrous (e.g. Sc, V, Zn) elements, incompatible elements (e.g. REE), and among SiO$_2$ or $Al_2$O$_3$ with ferrous elements, whereas negative correlations are appeared between Ni and major elements. These results involve increasing of the ferrous- and $Al_2$O$_3$-bearing minerals(e.g. amphibole and mica) with decreasing of Mg-bearing minerals (e.g. olivine) depending on the degree of alteration. Calculated geothermometries and mineral assemblages suggest that the ultramafic rocks have been metamorphosed through the condition from the greenschist to amphibolite facies. Compared with ultramafic rocks elsewhere, it is thought that those of the Hongseong and Kwangcheon areas are derivatives of the depleted sources since they are depleted in incompatible elements including REE abundances. Moreover overall characteristics of the ultramafic rocks are similar to the those of orogenic related Alpine type ultramafic rocks, especially, shallow mantle slab varieties.

A Study on Optimum Rate of Cattle Slurry Application for Forage Production using Idled Arable Land. I. The effect of applying time of cattle slurry and fertilization added mineral nitrogen on dry matter yield in Orchardgrass (유휴지에서 조사료 생산을 위한 적정 가축분뇨의 시용에 관한 연구 I. 액상구비의 시용시기와 무기태 질소의 첨가가 Orchardgrass의 건물수량에 미치는 영향)

  • Jo, Ik Hwan
    • Journal of the Korea Organic Resources Recycling Association
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    • v.2 no.2
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    • pp.65-75
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    • 1994
  • The aim of this experiment was to investigate the effects of applying time of cattle slurry and fertilizing added mineral nitrogen on dry matter yield of orchardgrass and also to estimate proper levels of fertilizing nitrogen when idled arable land rapidly increased these days, which was used for the production of Orchardgrass. The results were as follows. 1. The annual yields of dry matter were produced 5.62~6.67 ton per ha when cattle slurry applied at rates of $30m^3$(average mineral fertilizer equivalent to 120kg) per ha in different dressing times. Those were higher 0.60~1.65 ton or 0.16 ton per ha than the yields from non-fertilizing or phosphrous and potassium fertilizing. 2. Relative yields of annual dry matter from mineral nitrogen fertilization of 120kg per ha were 142~146% in comparsion with control plots(cattle slurry plot or phosphrous and potassium plot). On the other hand, those of cattle slurry application were 112~133% in comparion with non-fertilizing plot. 3. The efficiencies of nitrogen on dry matter yield(kg DM/kg N) were 5.0~13.8 DM kg/N kg when cattle slurry applied to Orchardgrass at rates of $30m^3$(average mineral fertilizer equivalent to 120kg) per ha, and were 21.3~23.1kg DM/kg N at the same level fertilization of mineral nitrogen. 4. On the condition of this study, mineral nitrogen were fertilized after applying cattle slurry at rates of $30m^3$(average mineral fertilizer equivalent to 120kg) per ha in order to minimize chemical fertilizer and increase the potential production of Orchardgrass. At this situation, marginal dry matter yields of 8.48~8.63 ton per ha were obtained as the level of economic even point goint when mineral nitrogen fertilization is between 119.4 and 133.3kg per ha. It was possible to obtain the maximal dry matter yield(8.98 tons/ha) with fertilization of 202.8kg N/ha.

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Analysis of Pesticide Residues in Rice Straw for Livestock Feed (사료용 볏짚 중 잔류농약 분석)

  • Gil, Geun-Hwan;Kim, Jin-Bae;Kim, Chan-Sub;Son, Gyeong-Ae;Gwon, Hye-Yeong;Park, Jea-Eup;Lee, Kyu-Seung
    • The Korean Journal of Pesticide Science
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    • v.16 no.4
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    • pp.273-281
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    • 2012
  • This study was conducted for the establishment of the analytical method of pesticide residues in rice straw for 9 pesticides; etofenprox, tricyclazole, diazinon, edifenphos, propiconazole, carbaryl, carbofuran, 3-hydroxy carbofuran and 3-keto carbofuran and for the monitoring of these pesticides in rice straw for livestock feed in Korea. These pesticides were classified into 4 groups according to analytical instrument condition. Group 1 (HPLC-UVD1) included tricyclazole and etofenprox while group 2 (HPLC-UVD2) included propiconazole and edifenphos. Group 3 (HPLC-FLD) included carbaryl, carbofuran, 3-hydroxy carbofuran and 3-keto carbofuran. Group 4 (GC-NPD) included Diazinon. The dried rice straw samples were extracted with acetone and purified by liquid-liquid partition and solid phase extraction (SPE): Combination of Florisil SPE and amino-propyl SPE was used for group 1 and group 2, amino-propyl SPE for group 3, and Florisil SPE was for group 4. Recovery was in the ranged 70~110% and the limits of quantitation (LOQ) were lower than the half of maximum residue limits. Therefore this method was proved to be efficient for monitoring of these pesticides residue in rice straw. A total of 18 rice straw samples from 6 provinces in Korea in 2010 were analyzed using established method and, only 3-keto carbofuran was detected in one sample at concentration of 0.04 mg/kg.

Analysis of Rainfall Infiltration Velocity in Unsaturated Soils Under Both Continuous and Repeated Rainfall Conditions by an Unsaturated Soil Column Test (불포화토 칼럼시험을 통한 연속강우와 반복강우의 강우침투속도 분석)

  • Park, Kyu-Bo;Chae, Byung-Gon;Park, Hyuck-Jin
    • The Journal of Engineering Geology
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    • v.21 no.2
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    • pp.133-145
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    • 2011
  • Unsaturated soil column tests were performed for weathered gneiss soil and weathered granite soil to assess the relationship between infiltration velocity and rainfall condition for different rainfall durations and for multiple rainfall events separated by dry periods of various lengths (herein, 'rainfall break duration'). The volumetric water content was measured using TDR (Time Domain Reflectometry) sensors at regular time intervals. For the column tests, rainfall intensity was 20 mm/h and we varied the rainfall duration and rainfall break duration. The unit weight of weathered gneiss soil was designed 1.21 $g/cm^3$, which is lower than the in situ unit weight without overflow in the column. The in situ unit weight for weathered granite soil was designed 1.35 $g/cm^3$. The initial infiltration velocity of precipitation for the two weathered soils under total amount of rainfall as much as 200 mm conditions was $2.090{\times}10^{-3}$ to $2.854{\times}10^{-3}$ cm/s and $1.692{\times}10^{-3}$ to $2.012{\times}10^{-3}$ cm/s, respectively. These rates are higher than the repeated-infiltration velocities of precipitation under total amount of rainfall as much as 100 mm conditions ($1.309{\times}10^{-3}$ to $1.871{\times}10^{-3}$ cm/s and $1.175{\times}10^{-3}$ to $1.581{\times}10^{-3}$ cm/s, respectively), because the amount of precipitation under 200 mm conditions is more than that under 100 mm conditions. The repeated-infiltration velocities of weathered gneiss soil and weathered granite soil were $1.309{\times}10^{-3}$ to $2.854{\times}10^{-3}$ cm/s and $1.175{\times}10^{-3}$ to $2.012{\times}10^{-3}$ cm/s, respectively, being higher than the first-infiltration velocities ($1.307{\times}10^{-2}$ to $1.718{\times}10^{-2}$ cm/s and $1.789{\times}10^{-2}$ to $2.070{\times}10^{-2}$ cm/s, respectively). The results reflect the effect of reduced matric suction due to a reduction in the amount of air in the soil.

Intracellular Concentrations of NAD(P), NAD(P)H, and ATP in a Simulated Oxic-settling-anaerobic (OSA) Process (OSA 공정의 세포 내 ATP, NAD(H), NADP(H) 농도)

  • Ventura, Jey-R Sabado;Nam, Ji-Hyun;Yang, Benqin;Na, Ri;Kil, Hyejin;Nam, Deok-Hyeon;Kang, Ki-Hoon;Jahng, Deokjin
    • Journal of Korean Society on Water Environment
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    • v.31 no.6
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    • pp.599-609
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    • 2015
  • In order to investigate why OSA (oxic-settling-anaerobic) process produces less sludge than CAS (conventional activated sludge) process, sequential cultivation through 1st aerobic-anaerobic-2nd aerobic conditions, were carried out. Then, the intracellular concentrations of adenosine triphosphate (ATP), nicotinamide adenine dinucleotide (NAD and NADH), and nicotinamide adenine dinucleotide phosphate (NADP and NADPH) were monitored for these three stages. Results showed that the concentrations of these energy substances rapidly decreased through time in both aerobic and anaerobic conditions but the anaerobic culture contained the lower energy level than aerobic culture. The 2nd aerobic culture that experienced anaerobic condition showed lower concentration of these energy substances than those of the 1st aerobic culture. Meanwhile, the anaerobic culture corresponding to the sludge holding stage of OSA was subjected to different soluble chemical oxygen demand (SCOD) levels, detention time, and temperature to evaluate the effects of these variations on the energy level difference between the 1st and 2nd aerobic stages. The lower the SCOD concentration, the longer detention time; and the higher temperature in the anaerobic stage tended to further reduce the intracellular level of the 2nd aerobic culture. On the average, the intracellular energy level of the anaerobic and 2nd aerobic stage were 57.73% and 39.12% of the 1st aerobic culture, respectively. These indicated that the insertion of an anaerobic stage between two aerobic stages could lower the intracellular energy levels, hence the lower the sludge in OSA than CAS process. Moreover, manipulation of the operating conditions of the intervening anaerobic stage can change intracellular energy levels thereby controlling sludge production.

Functional Brain Mapping Using $H_2^{15}O$ Positron Emission Tomography ( I ): Statistical Parametric Mapping Method ($H_2^{15}O$ 양전자단층촬영술을 이용한 뇌기능 지도 작성(I): 통계적 파라메터 지도작성법)

  • Lee, Dong-Soo;Lee, Jae-Sung;Kim, Kyeong-Min;Chung, June-Key;Lee, Myung-Chul
    • The Korean Journal of Nuclear Medicine
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    • v.32 no.3
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    • pp.225-237
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    • 1998
  • Purpose: We investigated the statistical methods to compose the functional brain map of human working memory and the principal factors that have an effect on the methods for localization. Materials and Methods: Repeated PET scans with successive four tasks, which consist of one control and three different activation tasks, were performed on six right-handed normal volunteers for 2 minutes after bolus injections of 925 MBq $H_2^{15}O$ at the intervals of 30 minutes. Image data were analyzed using SPM96 (Statistical Parametric Mapping) implemented with Matlab (Mathworks Inc., U.S.A.). Images from the same subject were spatially registered and were normalized using linear and nonlinear transformation methods. Significant difference between control and each activation state was estimated at every voxel based on the general linear model. Differences of global counts were removed using analysis of covariance (ANCOVA) with global activity as covariate. Using the mean and variance for each condition which was adjusted using ANCOVA, t-statistics was performed on every voxel To interpret the results more easily, t-values were transformed to the standard Gaussian distribution (Z-score). Results: All the subjects carried out the activation and control tests successfully. Average rate of correct answers was 95%. The numbers of activated blobs were 4 for verbal memory I, 9 for verbal memory II, 9 for visual memory, and 6 for conjunctive activation of these three tasks. The verbal working memory activates predominantly left-sided structures, and the visual memory activates the right hemisphere. Conclusion: We conclude that rCBF PET imaging and statistical parametric mapping method were useful in the localization of the brain regions for verbal and visual working memory.

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Studies on the grassland Development in the Forest IV. Possibility of the grassland improvement by spring sowing and microenvironmental conditions in the forest (임간초지 개발에 관한 연구 IV. 임간지에서 춘파초지개량 가능성과 주요 미기상 조사)

  • Park, M.S.;Han, Y.C.;Seo, S.;Lee, B.S.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.6 no.1
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    • pp.31-37
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    • 1986
  • In order to study the possibility of grassland improvement by spring sowing in the forest, microenvironmental conditions, emergence, percentage of grasses and weeds, root weight and dry matter yield of grasses were investigated. Two field sites (forest grassland and full-sunlight grassland) and two sowing times (March 20 and April 10) were assigned. The condition of the forest grassland was area of pine trees with 50% shading, and the experiment was performed at the Livestock Experiment Station in Suweon, 1984. The results obtained are summarized as follows: 1. For germination and early growth of grasses, full-sunlight grassland was more advantageous than forest grassland. Growth after that stage, on the other hand, forest grassland was more suitable. Especially, during dry and high temperature season, temperature of soil surface and underground in the forest grassland were decreased by $6-7^{\circ}C$ and $3-4^{\circ}C$ each other, compared with those of the full-sunlight grassland. Also soil moisture content was continuously higher in the forest grassland. 2. At March 20 sowing the emergence time in the full-sunlight grassland was shortened by 8 days, compared with that of the forest grassland. In case of sowing on April 10, however, there was no difference between two grassland sites. 3. Grasses grown in the forest was more prostrate and leaves from them decayed more, compared with those of the full-sunlight grassland. 4. The percentage of grasses in the forest grassland was 80 to 85 %, on the other hand, that of the full-sunlight grassland was only 15 to 20 %. And the percentage of grasses tended to be high in the plot of early sowing time. 5. Dry root weight and root length of grasses grown in the forest were inferior to those of the full-sunlight grassland (P<0.05), but there was no significant difference between two sowing times. 6. Dry matter yield of grasses was significantly higher (P<0.05) in the forest grassland than in the full-sunlight grassland, and yield was influenced by sowing time. Higher yield (4,011 kg/ha) was produced in the plot of the forest grassland with early spring sowing. 7. From above results, it is suggested that grassland improvement by spring sowing in the forest is possible, and it is desirable to sow in early spring.

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An Investigation on the Environmental Factors of Certified Organic and Non-pesticide Paddy Soils Cultivating Rice at Goseong-Gun (고성지역 유기농산물과 무농약농산물인증 논토양의 환경 조사)

  • Joo, Heui Sig;Cho, Young Son;Chun, Hyun Sik
    • Journal of Life Science
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    • v.24 no.4
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    • pp.403-410
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    • 2014
  • This study was conducted in organic certification soil for the comparison of heavy metals, nutrients, and irrigated water standards to certify a farm. It was carried out in 811 paddy fields of organic rice (Oryza sativa L.) cultivated at Goseong-Gun. The amounts of 8 heavy metals, Cd, Cu, As, Hg, Pb, $Cr^{6+}$, Zn, and Ni were found to be 0.05, 14.5, 1.08, 0.92, 10.7, 1.34, 35.9, and 22.2 mg $kg^{-1}$ in regular sequence in the organic paddy soil, and they were 0.32, 13.6, 1.01, 0.03, 10.4, 0.91, 42.4 and 22.5 mg $kg^{-1}$ in the non-pesticide paddy soil. In comparing organic and non-pesticide paddy soil with respect to the chemical characteristics of the soil, the average pH and the amount of organic matter, available phosphate and available silicate were 5.88 and 27.6 g $kg^{-1}$, 134.5 mg $kg^{-1}$, and 165.3 mg $kg^{-1}$, while they were 5.78 and 32.1 g $kg^{-1}$, 107.7 mg $kg^{-1}$, and 175.2 mg $kg^{-1}$, respectively. The amount of exchangeable cation $K^+$, $Ca^{2+}$, and $Mn^{2+}$ were 0.25, 5.20, and 1.04 cmol+ $kg^{-1}$ in organic paddy soil, while they were 0.38, 5.13, and 1.19 cmol+ $kg^{-1}$ in non-pesticide paddy soil. The pH, DO, BOD, COD and SS conditions of the irrigated water used in the organic paddy soil were found to be 7.23, 8.40, 2.80, 1.86, and 2.58 mg $l^{-1}$ and the condition of irrigated water used in the non-pesticide paddy soil were found to be 7.65, 9.16, 2.25, 4.11, and 4.00 mg $l^{-1}$, respectively. Based on these findings, we suggest that environmentally-friendly certificates in Korea have to unify organic and non-pesticide agro-products in an organic standard in food policy and control because there is no difference between soil and irrigated water standards in the two certifications.

Evaluation of the stability of IgM and specific antibody response of sevenband grouper Epinephelus septemfasciatus for application of antibody-detection ELISA (항체검출 ELISA 적용을 위한 능성어 IgM의 안정성 및 특이 항체 반응 평가)

  • Kim, Chun-Seob;Jang, Min-Seok;Kim, Wi-Sik;Kim, Jong-Oh;Kim, Du-Woon;Kim, Do-Hyung;Han, Hyun-Ja;Jeong, Sung-Ju;Oh, Myung-Joo
    • Journal of fish pathology
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    • v.22 no.3
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    • pp.335-342
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    • 2009
  • The stability of immunoglobulin M (IgM) on different serum storage conditions and specific antibody response were tested using the serum collected from sevenband grouper Epinephelus septemfasciatus by enzyme-linked immunosorbent assay (ELISA). To test the effect of storage temperature and duration, sevenband grouper antiserum against bovine serum albumin (BSA) was stored at -80, -20 or 4${^{\circ}C}$ for 1, 34, 61 or 119 days. In addition, to test the effect of repeated freeze-thawing condition, the anti-BSA fish serum was frozen at -20 and -80${^{\circ}C}$ and then thawn and frozen for 1, 5 or 10 times repeatedly. Consequently, no significant difference was found in ELISA optical density (O.D.) values of sera for the above mentioned storage conditions: different temperatures (-80, -20 and 4${^{\circ}C}$), durations of storage (1, 34, 61 and 119 days), and repeated thaw-freeze cycles (1, 5, and 10 times), indicating that IgMs of test fish were stable. The specific antibody response of sevenband grouper was observed after BSA-immunization of the test fish reared at 20 ${^{\circ}C}$ or 25${^{\circ}C}$. At the rearing temperature of 20${^{\circ}C}$, the specific antibody against BSA first appeared at 14 days and maximum antibody titer was observed between 21 and 28 days, while at the rearing temperature of 25 ${^{\circ}C}$, specific antibody appeared at 7 days and maximum antibody titer was observed between 14 and 21 days. In conclusion, the rearing temperature at 25${^{\circ}C}$ gave a faster and higher specific antibody response than at 20${^{\circ}C}$ and the specific antibody response maintained for approximately 2 months at 20℃ and 25${^{\circ}C}$.