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Evaluation of the stability of IgM and specific antibody response of sevenband grouper Epinephelus septemfasciatus for application of antibody-detection ELISA  

Kim, Chun-Seob (Division of Food Science and Aqualife Medicine, Chonnam National University)
Jang, Min-Seok (Division of Food Science and Aqualife Medicine, Chonnam National University)
Kim, Wi-Sik (Division of Food Science and Aqualife Medicine, Chonnam National University)
Kim, Jong-Oh (Division of Food Science and Aqualife Medicine, Chonnam National University)
Kim, Du-Woon (Division of Food Science and Aqualife Medicine, Chonnam National University)
Kim, Do-Hyung (Division of Food Science and Aqualife Medicine, Chonnam National University)
Han, Hyun-Ja (Division of Food Science and Aqualife Medicine, Chonnam National University)
Jeong, Sung-Ju (Division of Food Science and Aqualife Medicine, Chonnam National University)
Oh, Myung-Joo (Division of Food Science and Aqualife Medicine, Chonnam National University)
Publication Information
Journal of fish pathology / v.22, no.3, 2009 , pp. 335-342 More about this Journal
Abstract
The stability of immunoglobulin M (IgM) on different serum storage conditions and specific antibody response were tested using the serum collected from sevenband grouper Epinephelus septemfasciatus by enzyme-linked immunosorbent assay (ELISA). To test the effect of storage temperature and duration, sevenband grouper antiserum against bovine serum albumin (BSA) was stored at -80, -20 or 4${^{\circ}C}$ for 1, 34, 61 or 119 days. In addition, to test the effect of repeated freeze-thawing condition, the anti-BSA fish serum was frozen at -20 and -80${^{\circ}C}$ and then thawn and frozen for 1, 5 or 10 times repeatedly. Consequently, no significant difference was found in ELISA optical density (O.D.) values of sera for the above mentioned storage conditions: different temperatures (-80, -20 and 4${^{\circ}C}$), durations of storage (1, 34, 61 and 119 days), and repeated thaw-freeze cycles (1, 5, and 10 times), indicating that IgMs of test fish were stable. The specific antibody response of sevenband grouper was observed after BSA-immunization of the test fish reared at 20 ${^{\circ}C}$ or 25${^{\circ}C}$. At the rearing temperature of 20${^{\circ}C}$, the specific antibody against BSA first appeared at 14 days and maximum antibody titer was observed between 21 and 28 days, while at the rearing temperature of 25 ${^{\circ}C}$, specific antibody appeared at 7 days and maximum antibody titer was observed between 14 and 21 days. In conclusion, the rearing temperature at 25${^{\circ}C}$ gave a faster and higher specific antibody response than at 20${^{\circ}C}$ and the specific antibody response maintained for approximately 2 months at 20℃ and 25${^{\circ}C}$.
Keywords
Antibody detection; Enzyme-linked immunosorbent assay; Immunoglobulin M; Sevenband grouper
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Times Cited By KSCI : 2  (Citation Analysis)
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