Browse > Article

Evaluation of the stability of IgM and specific antibody response of sevenband grouper Epinephelus septemfasciatus for application of antibody-detection ELISA  

Kim, Chun-Seob (Division of Food Science and Aqualife Medicine, Chonnam National University)
Jang, Min-Seok (Division of Food Science and Aqualife Medicine, Chonnam National University)
Kim, Wi-Sik (Division of Food Science and Aqualife Medicine, Chonnam National University)
Kim, Jong-Oh (Division of Food Science and Aqualife Medicine, Chonnam National University)
Kim, Du-Woon (Division of Food Science and Aqualife Medicine, Chonnam National University)
Kim, Do-Hyung (Division of Food Science and Aqualife Medicine, Chonnam National University)
Han, Hyun-Ja (Division of Food Science and Aqualife Medicine, Chonnam National University)
Jeong, Sung-Ju (Division of Food Science and Aqualife Medicine, Chonnam National University)
Oh, Myung-Joo (Division of Food Science and Aqualife Medicine, Chonnam National University)
Publication Information
Journal of fish pathology / v.22, no.3, 2009 , pp. 335-342 More about this Journal
Abstract
The stability of immunoglobulin M (IgM) on different serum storage conditions and specific antibody response were tested using the serum collected from sevenband grouper Epinephelus septemfasciatus by enzyme-linked immunosorbent assay (ELISA). To test the effect of storage temperature and duration, sevenband grouper antiserum against bovine serum albumin (BSA) was stored at -80, -20 or 4${^{\circ}C}$ for 1, 34, 61 or 119 days. In addition, to test the effect of repeated freeze-thawing condition, the anti-BSA fish serum was frozen at -20 and -80${^{\circ}C}$ and then thawn and frozen for 1, 5 or 10 times repeatedly. Consequently, no significant difference was found in ELISA optical density (O.D.) values of sera for the above mentioned storage conditions: different temperatures (-80, -20 and 4${^{\circ}C}$), durations of storage (1, 34, 61 and 119 days), and repeated thaw-freeze cycles (1, 5, and 10 times), indicating that IgMs of test fish were stable. The specific antibody response of sevenband grouper was observed after BSA-immunization of the test fish reared at 20 ${^{\circ}C}$ or 25${^{\circ}C}$. At the rearing temperature of 20${^{\circ}C}$, the specific antibody against BSA first appeared at 14 days and maximum antibody titer was observed between 21 and 28 days, while at the rearing temperature of 25 ${^{\circ}C}$, specific antibody appeared at 7 days and maximum antibody titer was observed between 14 and 21 days. In conclusion, the rearing temperature at 25${^{\circ}C}$ gave a faster and higher specific antibody response than at 20${^{\circ}C}$ and the specific antibody response maintained for approximately 2 months at 20℃ and 25${^{\circ}C}$.
Keywords
Antibody detection; Enzyme-linked immunosorbent assay; Immunoglobulin M; Sevenband grouper
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Kim, W.S., Mochizuki, M., Nishizawa, T. and Yoshimizu, M.: Detection of specific antibodies against infectious hematopoietic necrosis virus from rainbow trout sera by ELISA using two novirhabdoviruses. Fish Pathol., 43:112-116, 2008   DOI
2 Shi, Y., Yi, Y., Li, P., Kuang, T., Li, L., Dong, M., Ma, Q. and Cao, C.: Diagnosis of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid antibodies in an antgencapturing enzyme-linked immunosorbent assay. J. Clin. Microbiol., 41: 5781-5782, 2003   DOI   ScienceOn
3 Wilson, M.R. and Warr, G.W.: Fish immunoglobulins and the genes that encode them. Annu. Rev. Fish Dis., 201-221, 1992   DOI   ScienceOn
4 손상규, 박명애, 오명주, 전세규.: 양식 능성어로부터 fish nodavirus 분리. 한국어병학회지, 11:97-104, 1998
5 奧田律子: サクラマスのスモルト生産における細菌性腎臟病原因菌の感染環の形成 とその防除對策に關する硏究. 博士學位論文, 北海道大學, 日本, 2007
6 방종득, 박수일.: 넙치의 면역 반응에 수온이 미치는 영향. 한국어병학회지, 7:119-126, 1994
7 LaPatra, S.E.: The use of serological techniques for virus surveillance and certification of finfish. Annu. Rev. Fish Dis., 6: 15-28, 1996   DOI   ScienceOn
8 Corbel, M.J.: The immune response in fish: a review. J. Fish Biol., 7:539-563, 1975   DOI
9 De Marco, M.A., Foni, E., Campitelli, L., Raffini, E., Delogu, M. and Donatelli, I.: Long-term monitoring for avian influenza viruses in wild bird species in Italy. Vet. Res. Commun., 27:107-114, 2003   DOI   ScienceOn
10 Fijan, N., Petrinec, Z., Stancl, Z., Dorson, M. and LeBerre, M.: Hyperimmunization of carp with Rhabdovirus carpio. Bull. Off. Int. Epizoot., 87:439-440, 1977
11 김위식, 장민석, 김종오, 김두운, 정성주, 김석렬, 박명애, 오명주.: 넙치 혈청의 보존 조건이 ELISA 결과에 미치는 영향. 한국어병학회지, 22: 167-172. 2009   ScienceOn
12 원호근, 김길동, 신은경, 강정무, 김현일, 조윤상, 한태욱.: 기니픽에서 Haemophilus parasuis 백신 (글래써씨병 백신)의 항체가 측정을 위한 ELISA기법 개발. 한국수의공중보건학회지, 29:65-72, 2005
13 粉川愉記: コイヘルペスウイルス(KHV)に對する抗を檢出するためのELISA法に 關する硏究. 修士學位論文, 北海道大學, 日本, 2006
14 Nitzan, S., Shwartsburd, B. and Hellerb, E.D.: The effect of storing hybrid bass (Morone saxatilis × M. chrysops) sera at freezing temperatures on antibody titers. Fish Shellfish Immunol., 15: 341-347, 2003   DOI   ScienceOn
15 Suarez, D.L. and Schultz-Cherry, S.: Immunology of avian influenza virus: a review. Dev. Comp. Immunol., 24: 269-283, 2000   DOI   ScienceOn
16 손상규, 전세규.: 능성어, Epinephelus septemfasciatus의 바이러스성 신경괴사증 바이러스의 병원성 연구. 한국어병학회지, 12: 107-113, 1999
17 Kollner, B., Wasserrb, B., Kotterba, G. and Fisher, U.: Evaluation of immune functions of rainbow trout (Oncorhynchus mykiss) - how can environmental influences be detected?. Toxicology Letters, 131:83-95, 2002   DOI   ScienceOn
18 Renzullo, P.O., Sateren, W.B., Garner, R.P., Milazzo, M.J., Birx, D.L. and McNeil, J.G. : HIV-1 seroconversion in united states army active duty personnel, 1985-1999. AIDS, 15:1569- 1574, 2001   DOI   ScienceOn
19 Yoshimizu, M., Direkbusarakom, S., Nomura, T., Ezura, Y. and Kimura, T.: Detection of antibody against Aeromonas salmonicida in the serum of salmonid fish by the enzyme linked immunosorbent assay. Fish Pathol., 27: 73-82, 1992   DOI
20 Kim, W.S., Nishizawa, T. and Yoshimizu, M.: Nonspecific adsorption of fish immunoglobulin M (IgM) to blocking reagents on ELISA plate wells. Dis. Aquat. Org., 78:55-59, 2007   DOI   ScienceOn