• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1998.05a
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• pp.153-157
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• 1998

무등산 수박의 유묘 생산에 있어 적정 배양액 농도를 구명하기 위하여 일본원시균형배양액을 기준으로 하여 NO$_3$-N, K 및 Ca의 농도별 첨가량에 따른 유묘생장반응을 조사하였다. 1. 배양액내 질소농도을 증가시킬수록 초장, 엽면적, 엽수, 엽장, 엽폭이 증가하였으며 생체중과 건물중도 증가하였다. 2. 배양액내 칼륨의 농도를 증가시켰을 경우 수박 유묘의 초장은 200ppm까지는 약간 증가하지만 엽면적, 엽수, 엽장, 생체중 및 건물중은 200ppm이상으로 증가시키면 감소하는 경향이었다. 3. 칼슘처리의 경우 농도의 증가가 생장의 증가를 나타내지는 않았으며 원시균형 배양액의 표준이라 할 수 있는 80ppm처리구의 생장이 양호한 것으로 나타났다. 4. 배양액(일본원시균형배양액 1/2농도)에 N의 농도를 200ppm으로 증가시킬 경우 수박유묘의 엽병내 N, K 및 Mg의 함량이 증가하였지만 P 및 Ca의 증가나 감소의 경향은 보이지 않았다. 5. K의 농도를 100ppm으로 증가시킬 경우 수박유묘의 엽병내 N, K 및 Mg의 함량이 증가하였지만 P 및 Ca의 증가나 감소의 경향은 보이지 않았다. 5. K의 농도를 150ppm으로 증가시킬 경우 수박유묘의 엽병내 N, K 및 Mg의 함량이 증가하였지만 P 및 Ca의 증가나 감소의 경향은 보이지 않았다. 그러나 200ppm이상으로 증가시킬 경우 N과 Mg의 감소가 나타났다. 6. 양액내 Ca의 농도를 증가시킬수록 엽병내 N, K Ca 및 Mg의 농도가 증가하는 반면 P의 경우 거의 변화가 없었으며,N의 경우는 200ppm 이상의 농도에서 감소하는 경향을 나타내었다. Mg의 경우는 150ppm까지는 함량의 변화가 없었지만 200ppm 이상의 처리구에서 급격한 함량증가를 나타내었다.~21.2ml가 흘러나와 배지의 공극이 일정하지 않아 시간당 통과하는 양이 일정하지 않았다고 생각되었다. $\bigcirc$ 펄라이트 : 합섬A(비스코스+레이온)급액천의 유입은 소(1$\times$60cm)에서 21.8ml, 중(2$\times$60cm) 33.5ml, 대(3$\times$60cm) 43.4ml가 통과되었고 합섬(폴리에스텔)에서는 19.0~30.7ml로서 급액천의 규격에 따라 통과되는 차이가 있었다. 배지가 규격화되어 있어 급액천의 규격별로 일정하게 유입되었으며 급액천의 재질이 유입에 영향을 미친 것으로 사료되었다. (2) 급액관과 베드상과의 높이에 따른 유출양 : 급액과 베드상과의 낙차가 클수록 유출이 증가함을 알수 있었으나 합섬C(인견)실험구에서는 낙차가 유출에 영향을 미치지 않았다. (4) 급액된 양액의 EC 및 pH조사 : 급액된 양액의 EC 및 pH에 전혀 변화가 없어 재배 적응에 문제가 없을것으로 사료되었다.이가 가장 이상적인 것으로 생각된다.세포수에 대한 내부세포괴세포(ICM/total cells)가 20~40% 범주에 드는 비율은 처리구가 대조구보다 낮은 결과를 나타냈다. 결론적으로 돼지난포란을 이용하여 체외성숙을 유기할 때 효과적인 cysteamine의 농도는 50$\mu$M이 적당하며, 초기배발달을 유기할 때의 효과적인 cysteamine의 농도는 25~50$\mu$M인 것으로 판단된다.N)A(N)/N을 제시하였다(A(N)=N에 대한 A값). 위의 실험식을 사용하여 헝가리산 Zempleni 시료(15%$S_{XRD}$)의 기본입자분포로부터 %$S_{XRD}$를 계산한

• Analytical Science and Technology
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• v.28 no.6
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• pp.436-445
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• 2015

Amomi Fructus with anti-oxidative activity was chosen and essential oil was obtained by SDE (simultaneous distillation extraction), and 39 constituents were determined by GC-MS (gas chromatography-mass spectrometry). Major components were camphor, borneol acetate, borneol, D-limonene and camphene. Three solvent extracts such as hexanes, diethyl ether and methylene chloride from Amomi Fructus were obtained. These were analyzed by GC-MS and 4 more constituents were identified in addition to 39 components discovered in essential oil. Five major components such as camphor, borneol acetate, borneol, D-limonene and camphene were also detected, however the relative peak percents of those components were different from those of constituents in essential oil. To estimate the kind and the amount of materials evaporated at certain temperature and conditions from essential oil and solvent extracts, dynamic headspace apparatus was used and materials evaporated and trapped at certain conditions were analyzed by GC-MS. Recovery yield of SDE method from Amomi Fructus was measured by using camphor and standard calibration solution of camphor methanol solution and, the yield was 82.0%. Content of Hg was measured by mercury analyzer and contents of Cd, Pb, Cr, Mn, Co, Ni, Cu and Zn in Amomi Fructus, essential oils and solvent extracts were determined by ICP-MS (Inductively coupled plasma-mass spectrometer). Pb, Cd and Hg were measured in the concentration of 0.72 mg/kg, <0.10 mg/kg and 0.0023 mg/kg, respectively and these were below permission level of purity test. Contents of Mn, Cu and Zn in Amomi Fructus were 213 mg/kg, 8.29 mg/kg and 31.0 mg/kg, respectively and which were relatively higher than other metals such as Cr, Co and Ni. Metals such as Mn (0.65 ~ 9.08 mg/kg), Cu (1.16 ~ 4.40 mg/kg) and Zn (1.10 ~ 3.80 mg/kg) in essential oil and solvent extracts were detected. At this point it is not clear that the metals were cross-contaminated in the course of treating Amomi Fructus or metals were contained in Amomi Fructus. The influence evaluation toward biological model study of these metals in essential oil and solvent extracts will be needed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.6
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• pp.547-557
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• 1986

The characteristics of the three alkaline proteinases, Enz. A, B and C, from the pyloric caeca of mackerel have been investigated. The optimum condition for the activity of the Enz. A, B and C was pH 9.4, 9.8 and 9.8 at $45^{\circ}C$ for $2\%$ casein solution, and was pH 9.2 10.2 and 9.8 at $45^{\circ}C$ for $5\%$ hemoglobin denatured by urea, respectively. Enz. A, B and C by heat treatment at $50^{\circ}C$ for 5 min were inactivated 90, 33 and $37\%$, respectively, over the original activity. The reaction rate of the three alkaline proteinases was constant to the reaction time to 40 min in the reaction condition of $2{\mu}g/ml$ of enzyme concentration and $2\%$ casein solution. The reaction rate equation and Km value against casein substrate determined by the method of Lineweaver and Burk were: Enz. A, Y=3.6X and $Km=5.0{\times}10^{-3}\%$; Enz. B, Y=6.0X and $Km=1.0{\times}10^{-3}\%$; Enz. C, Y=4.2X and $Km=3.6{\times}10^{-3}\%$. The three alkaline proteinases were inactivated by $Ag^+$ and $Hg^{2+}$, but activated by $Mn^{2+},\;Sn^{2+}\;and\;Pb^{2+}$, Enz. B and C were remarkably inhibited by the soybean trypsin inhibitor. Molecular weight of Enz. A, B and C determined by SDS-polyacrylamide gel electrophoresis and Sephadex G-100 gel filtration was in the range of $27,500{\pm}2,500,\;20,500{\pm}1,500\;and\;15,250{\pm}250$, respectively.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.11
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• pp.812-820
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• 2011

This study was conducted to characterize $CO_2$ biomineralization on several minerals (i.e., CaO, MgO, $SiO_2$) by bottle test in an aqueous solution and solidified sludge using different aerobic bacterial strains like Bacillus megaterium and Bacillus pasteurii by batch test. These bacteria promote the formation of microenvironments that facilitate the precipitation of mineral phases that were unsaturated in the bulk solution. For one type of mineral solely amended, the $CO_2$ was reduced at the highest of 4.0 mmol for MgO while it was not that much lower for CaO and $SiO_2$ showing 1.1 and 0.3 mmol $CO_2$2, respectively. For two types of minerals simultaneously amended, the $CO_2$ was reduced at the greater extent for both Ca + Mg and Mg + Si showing 2.7 and 2.3 mmol, respectively whereas it was less for Ca + Si at 1.8 mmol. For solidified sludge, the $CO_2$ reduction rate changed depending on the volume of solidified sludge placed in the medium and the input $CO_2$ concentration.. The reduction rate of $CO_2$ was increased with increasing the volume of solidified sludge. Results of XRD analysis indicate that $CaCO_3$ (Calcite) was dominantly formed among others (e.g., Aragonite, Dolomite). SEM analysis showed that the sample with Bacillus pasteurii, could more form minerals rather than control. As demonstrated in this study, $CO_2$ would be effectively sequestered in biomineralization process.

• Food Engineering Progress
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• v.21 no.4
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• pp.360-366
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• 2017

Fish skin peptide-loaded liposomes were prepared in 100 mL and 1 L solution as lab scales, and 10 L solution as a prototype scale. The particle size and zeta potential were measured to determine the optimal conditions for the production of fish skin peptide-loaded liposome. The liposome was manufactured by the following conditions: (1) primary homogenization at 4,000 rpm, 8,000 rpm, and 12,000 rpm for 3 minutes; (2) secondary homogenization at 40 watt (W), 60 W, and 80 W for 3 minutes. From this experimental design, the optimal conditions of homogenization were selected as 4,000 rpm and 60 W. For the next step, fish peptides were prepared as the concentrations of 3, 6, and 12% at the optimum manufacturing conditions of liposome and stored at $4^{\circ}C$. Particle size, polydispersion index (pdI), and zeta potential of peptide-loaded liposome were measured for its stability. Particle size increased significantly as manufacture scale and peptide concentration increased, and decreased over storage time. The zeta potential results increased as storage time increased at 10 L scale. In addition, 12% peptide showed the formation of a sediment layer after 3 weeks, and 6% peptide was considered to be the most suitable for industrial application.

• Journal of Bio-Environment Control
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• v.13 no.4
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• pp.233-239
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• 2004

This research was conducted to determine the effect of soil pH change followed by leaching with ground water on crop growth, yield and nutrient uptake of strawberry. Treatments were leaching with ground water (A), hydrated lime application of dissolved portion in $500g{\cdot}L^{-1}$ solution followed by leaching (B), application of $Al_2SO_4$ solution with the concentration of $0.75g{\cdot}L^{-1}$ followed by leaching (C), pH elevation by (B) followed by pH lowering by (C), then leaching (D). Soil samples taken before experiment had 6.0 in soil pH. But the pH of all treatments taken after pH changes and leaching were in the range from 5.8 to 6.2 without statistical differences among treatments. Electrical conductivity of soils taken before experiment was $1.82dS{\cdot}m^{-1}$ and those of (A), (B), (C), and (D) were 1.65, 1.72, 1.71 and $1.83dS{\cdot}m^{-1}$, respectively. The plant height in treatment (C) was 20.4 cm at 80 days after transplanting which was the highest among treatments tested. The other growth characteristics did not show statistical differences among treatments. Tissue contents of Fe, Mn, Zn, Cu and B in treatment (C) were higher than those of other treatments at 80 days after transplanting. The treatment (C) had the highest number of special sizes and fruit weight per 50 plants such as 137 and 3.784 g, respectively. The treatment also had 3.123 kg in total fruit weight and 2,489 kg in marketable fruit weight per 10 are. The treatment (B) also had good results such as 2,820 kg in marketable fruit weight per 10 are.

• Analytical Science and Technology
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• v.9 no.4
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• pp.336-344
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• 1996

The extraction of trace cobalt, copper, nickel, cadmium, lead and zinc in urine samples of organic and alkali metal matrix into chloroform by the complex with a dithizone was studied for graphite furnace AAS determination. Various experimental conditions such as the pretreatment of urine, the pH of sample solution, and dithizone concentration in a solvent were optimized for the effective extraction, and some essential conditions were also studied for the back-extraction and digestion as well. All organic materials in 100 mL urine were destructed by the digestion with conc. $HNO_3$ 30 mL and 30% $H_2O_2$ 50 mL. Here, $H_2O_2$ was added dropwise with each 5.0 mL, serially. Analytes were extracted into 15.0 mL chloroform of 0.1% dithizone from the digested urine at pH 8.0 by shaking for 90 minutes. The pH was adjusted with a commercial buffer solution. Among analytes, cadmium, lead and zinc were back-extracted to 10.00 mL of 0.2 M $HNO_3$ from the solvent for the determination, and after the organic solvent was evaporated, others were dissolved with $HNO_3-H_2O_2$ and diluted to 10.00 mL with a deionized water. Synthetic digested urines were used to obtain optimum conditions and to plot calibration-eurves. Average recoveries of 77 to 109% for each element were obtained in sample solutions in which given amounts of analytes were added, and detection limits were Cd 0.09, Pb 0.59, Zn 0.18, Co 0.24, Cu 1.3 and Ni 1.7 ng/mL, respectively. It was concluded that this method could be applied for the determination of heavy elements in urine samples without any interferences of organic materials and major alkaline elements.

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.1-8
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• 2011

Recently the increasing of vinyl and green houses and development of reclaimed land including Saemangeum induced the need for breeding salt-tolerant crops which can survive and grow in high salinity soil. So we try to develop salt-tolerant transgenic chrysanthemum (Dendranthema grandiflorum.) lines by using anti-porter gene TANHX and HVNHX. Through marker selection and plant regeneration step, we could get 284 putative transgenic chrysanthemum lines. On selected putative transgenic plants, 40 candidates were used for genetic analysis and 30 lines could be made up of target size band on PCR, so about 75% of marker selected lines were decided as real transgenic lines. Selected 284 transgenic lines were also used for salt-tolerance test as a range of NaCl 0.2 ~ 1.2% (300 mM). As a result of salt-tolerance test, 15 selected transgenic lines could live and grow on the continuous supply of 0.8% (200 mM) NaCl solution and another 7 lines were could survive under 1.2% (300 mM) NaCl solution. This salt-tolerant transgenic lines under salt stress also lead a cell alternation especially a guard cell. A stressed guard cell be swelled and grow larger in proportion to NaCl concentration. TTC test for cell viability on transgenic chrysanthemum lines pointed out that more strong salt-tolerant lines can be live more than another under same salt stress. The numerical value of strong salt-tolerant 7 transgenic lines were 0.206 ~ 0.331 under 1.2% NaCl stress, and then it's value is more larger than middle salinity lines' 0.114 ~ 0.193 and non-transgenic's 0.046. And the proline contents as indicated stress compound also pointed out that HVNHX introduced salt-tolerant transgenic lines were less stressed than other under same salt stress. The contents of strong salt-tolerant transgenic lines were 2.255 ~ 2.638 mg/kg and it is much higher than that of middle salinity lines' 1.496 ~ 2.125.

• Journal of Technologic Dentistry
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• v.19 no.1
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• pp.21-35
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• 1997

The Ag-Pd-Cu alloys containing a small amount of Au is commonly used for dental purposes, because this alloy is cheaper than Au-base alloys for clinical use. However, the most important characteristic of this alloy is age-hardenability, which is not exhibited by other Ag-base dental alloys. The specimens used were Ag-20Pd-20Cu ternary alloy and Au addition alloy. These alloys were melted and casted by induction electic furace and centrifugal casting machine in Ar atmoshpere. These specimens were solution treated for 2hr at $800^{\circ}C$ and were then quenched into iced water, and aged at $350{\sim}550^{\circ}C$ Age-hardening characteristics of the small Au-containing Ag-pPd-Cu dental alloys were investigated by means of hardness testing, X-ray diffraction and electron microscope observations, electrical resistance, differential scanning calorimetric, emergy dispersed spectra and electron probe microanalysis. Principal results are as follows : Hardening occured in two stages, I. e., stage I in low temperature and stage II in high temperature regions, during continuous aging. The case of hardening in stage I was due to the formation of the Llo type face centered tetragonal PdCu-ordered phase in the grain interior and hardening in stage I was affedted by the Cu concentration. In stage II, decomposition of the $\alpha$ solid solution to a PdCu ordered phase(L1o type) and an Agrich ${\alpha}2$ phase occurred and a discontiunous precipitation occurred at the grain boundary. Form the electron microscope study, it was concluded that the cause of age-hardening in this alloy is the precipitation of the PdCu ordered phase, which has AuCu I type face-centered tetragonal structure. Precipitation procedure was ${\alpha}\to{\alpha}+{\alpha}2+PdCu\to{\alpha}1+{\alpha}2+PdCu$ at Pd/Cu = 1 Ag-Pd-Cu alloy is more effective dental alloy as ageing treatment and is suitable to isothermal ageing at $450^{\circ}C$.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.409-415
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• 2022

Plant growth and development are also affected by root-zone environment. Therefore, it is important to consider the variables of the root-zone environment when establishing an irrigation strategy. The purpose of this study is to analyze the relationship between the volumetric moisture content (VWC), Bulk EC (ECb), and Pore EC (ECp) used by plant roots using FDR sensors in two types of rockwool media with different water transmission characteristics, using the method above this was used to establish a method for collecting and correcting available root-zone environmental data. For the experiment, two types of rockwool medium (RW1, RW2) with different physical characteristics were used. The moisture content (MC) and ECb were measured using an FDR sensor, ECp was measured after extracting the residual nutrient solution from the medium using a disposable syringe in the center of the medium at a volumetric moisture content (VWC) of 10-100%. Then, ECb and ECp are measured by supplying nutrient solution having different concentration (distilled water, 0.5-5.0) to two types of media (RW1, RW2) in each volume water content range (0 to 100%). The relationship between ECb and ECp in RW1 and RW2 media is best suited for cubic polynomial. The relationship between ECb and ECp according to volume moisture content (VWC) range showed a large error rate in the low volume moisture content (VWC) range of 10-60%. The correlation between the sensor measured value (ECb) and the ECp used by plant roots according to the volumetric water content (VWC) range was the most suitable for the Paraboloid equation in both media (RW1, RW2). The coefficient of determination the calibration equation for RW1 and RW2 media were 0.936, 0.947, respectively.