• Title/Summary/Keyword: concentration of nitrite

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Effect of sodium nitrite on Trichomonas vaginalis (아질산나트륨(sodium nitrite)이 질편모충 증식과 미세구조에 미치는 영향)

  • Ryu, Jae-Suk;Buk, Jung-Hwa;Min, Deuk-Yeong
    • Parasites, Hosts and Diseases
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    • v.33 no.4
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    • pp.349-356
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    • 1995
  • We have investigated the action of sodium nitrite on the growth and morphologic changes of T uaginolis and on the treatment of subcutaneous abscess by trichomonad in mice. Sodium nitrite inhibited the growth of metronidazole-sensitive KT9 isolate and metronidazole-resistant CDC85 strain of T vcsinalis as concentration of 6 mM and 10 mM respectively Intraperitoneal injection of sodium nitrite (70 ㎍, 100 ㎍, 130 ㎍/g body weight) did not reduce the size of abscess produced by subcutaneous inoculation of T uasinnlis in mice. T uosinnlis, treated with sodium nitrite at concentration giving about 50% inhibition of growth, showed fissures, many vacuoles and electron-translucent zone in the cytoplasm by transmission electron microscopy. In the case of CDC85 treated with 9 mM sodium nitrite, hydrogenosomal matrical change , destruction of hydrogenosomal membrane, autophagic vacuoles, disappearance of Golgi complex and polysome were notably observed . With above results, it is assumed that sodium nitrite inhibits the growth of metronidazole-sensitive and - resistant strains of T. ucsinalis and induces the morphological changes of 7 uusinalis although it does not affect in reducing of abscess size by vagiginalis in mice.

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Biofilm airlift 반응기를 이용한 선택적 질산화의 연구

  • Yun, Ho-Jun;Jang, Jae-Seon;Kim, Dong-Jin
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.457-460
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    • 2000
  • A biofilm airlift reactor filled with biomass-covered carriers (sand) were used to remove ammonium by selective nitrification (ammonium to nitrite). The effects of experimental conditions (ammonium load, pH, dissolved oxygen) on nitrification and nitrite accumulation were investigated. The reactor showed more than 90% nitrification efficiencies at 2.5 kg $NH_4\;^+-N/m^3/d$ and $NO_2\;^--N$ could be accumulated between 75% and 90% in the effluent. It is likely that nitratation (nitrite oxidizer) was inhibited by low dissolved oxygen concentration while nitritation (ammonium oxidizer) was kept stable.

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Effect of Substituted Conjugated Linoleic Acid for Fat on Meat Qualities, Lipid Oxidation and Residual Nitrite Content in Emulsion-type Sausage

  • Hah, K.H.;Yang, H.S.;Hur, S.J.;Moon, S.S.;Ha, Y.L.;Park, G.B.;Joo, Seon-Tea
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.5
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    • pp.744-750
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    • 2006
  • An experiment was carried out to evaluate the effects of substituted conjugated linoleic acid (CLA) for fat on qualities, lipid oxidation and residual nitrite contents in emulsion-type sausage (ETS) after different periods of storage. The control sausage contained 20% fat, however, the substituted CLA for fat sausages contained 17.5% fat+2.5% CLA, 15% fat+5% CLA, 12.5% fat+7.5% CLA and 10% fat+10% CLA, respectively. CLA concentration was significantly increased (p<0.05) by substitution of CLA sources for fat, and storage did not affect the CLA concentration. TBARS were significantly increased with the increasing of storage time in all treatments (p<0.05). Significantly higher $L^*$ values were observed in the 10% CLA treatment compared to other treatments during storage. Moreover, $a^*$ values in CLA treatments significantly (p<0.05) increased compared to the control. Again, $b^*$ values were significantly (p<0.05) higher in CLA treatments than those of the control during storage. The concentration of residual nitrite in ETS was significantly (p<0.05) different between the treatments during storage and the results were in the following order: control >2.5% CLA>5% CLA and 7.5% CLA>10% CLA. Overall acceptability of CLA treatments was significantly (p<0.05) lower than the control during storage, whereas no significant differences were found in overall acceptability between the treatments after 28 days of storage.

Antioxidant and Anti-inflammatory activity of Colpomenia sinuosa extract (불레기말 추출물의 항산화 및 항염효과)

  • Cho, Young Jae;Kim, Sook-hee;Choi, Jae Young;Lee, Ja-bok
    • Journal of Convergence for Information Technology
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    • v.12 no.1
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    • pp.135-143
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    • 2022
  • In this study, the antioxidant and anti-inflammatory properties of Colpomenia sinuosa extracts were identified. Antioxidant experiments included DPPH, ABTS, nitrite scavenging experiments, and FRAP, polyphenol concentration measurements, flavonoid concentration measurements. DPPH assay results showed an antioxidant function of 2.821 mg ascorbic acid/g extract. ABTS assay results showed an antioxidant function of 3.923 mg ascorbic acid/g extract. nitrite assay results showed an antioxidant function of 17.89 mg ascorbic acid/g extract. In FRAP, 1 mg of the Colpomenia sinuosa extract showed a reduction of 2.062±0.177 ㎍ of ascorbic acid. For polyphenols, 62.85±3.18 mg/g was shown. Flavonoids showed 10.01±0.24 mg/g. In the meantime, cell experiments showed cytotoxicity and anti-inflammatory functions. In cytotoxicity experiments, Colpomenia sinuosa extracts showed cytotoxicity of less than 20% and an inflammatory inhibition of 30.93±2.93% at a concentration of 100 ㎍/mL. These results indicate that Colpomenia sinuosa extract is available as functional cosmetic material.

Change in Quality Properties of Extracts from Roasted Pleurotus eryngii by Microwave-Assisted Extraction Condition (마이크로웨이브 추출조건에 따른 볶음 새송이버섯의 품질 특성 변화)

  • Yoon, Sung-Ran;Lee, Myung-Hee;Kim, Hyun-Ku;Lee, Gee-Dong
    • Food Science and Preservation
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    • v.13 no.6
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    • pp.732-739
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    • 2006
  • This study was performed to investigate the changes in quality properties of extract from roasted Pleurotus eryngii by microwave-assisted extraction (MAE). MAE was carried out under different conditions including ethanol concentration, extraction time and microwave power. Total phenolics compound contents, electron donating ability (EDA) and nitrite-scavenging ability at pH 1.2 were high at 50% ethanol concentration. Superoxide dismutase (SOD)-like activity was high at 75% ethanol concentration. Soluble solid and total phenolics compound content decreased as extending extraction time up to 7 min. EDA decreased as increased extraction time. SOD-like activity and nitrite-scavenging ability were not significantly different. The highest soluble solid content, total phenolics compound content and EDA were obtained at 100 W extraction of microwave power. SOD-like activity was high at 25 W. But microwave power did not significantly affect the nitrite-scavenging ability.

Nitrite-Scavenging and Antioxidant Activities of Wood Vinegar (목초액의 아질산염 소거 및 항산화 활성)

  • 정창호;심기환
    • Food Science and Preservation
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    • v.9 no.3
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    • pp.351-355
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    • 2002
  • The purpose of this study was to investigate the nitrite scavenging, eletron donating activity and antioxidant activity of wood vinegar on soybean oil. Wood vinegar was added to soybean oil in the quantities of 0.5, 1 and 5%. Nitrite scavenging effect of all concentrations diminished at higher pH, while in the case of pH 1.2, it showed a nitrite scavenging effect of more than 80% at concentration above of 100 ㎕/mL. The electron donating activity was about 80% at concentration above of 50 ㎕/mL. Soybean oil without the addition of wood vinegar was used as a control. The oxidation of these samples was determined by measuring the peroxide value(POV), thiobarbituric acid(TBA) value and acid value(AV). When wood vinegar was added 1 and 5%, the peroxide value wag lower than both the control and 0.1% BHT. TBA value were in the 5% wood vinegar tallowed by 1% wood vinegar, 0.1% BHT, 0.5% wood vinegar and the control, respectively. The results showed that the acid values were lowest in 5% wood vinegar, followed by the 0.1% BHT, 1% wood vinegar, 0.5% wood vinegar and finally the control.

Effects of natural nitrite source from Swiss chard on quality characteristics of cured pork loin

  • Kim, Tae-Kyung;Hwang, Ko-Eun;Song, Dong-Heon;Ham, Youn-Kyung;Kim, Young-Boong;Paik, Hyun-Dong;Choi, Yun-Sang
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.12
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    • pp.1933-1941
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    • 2019
  • Objective: This study was conducted to evaluate quality characteristics of cured pork loin with natural nitrite source from Swiss chard. Methods: Pork loin was cured in brine and the ratio of water and fermented Swiss chard (FSC) solution in the brine was changed by 4:0 (control), 3:1 (T1), 1:1 (T2), 1:3 (T3), 0:4 (T4), and pickled samples with 0.012% sodium nitrite (PC, positive control) and nitrite free brine (NC, negative control) were considered as the control. Results: The pH values of cured pork loins with FSC were decreased with increasing addition level of FSC. Cooking loss was not significantly different among all treatments. T4 had the lowest value in moisture content and lightness value and the highest value in curing efficiency. The redness value of T4 was not significantly different from that of PC in raw. After cooking, however, it was higher than that of PC. The yellowness value of cured pork loin added with FSC was increased with increasing level of FSC. Volatile basic nitrogen content of cured pork loin added with FSC was higher than PC and NC. Thiobarbituric acid reactive substance value of cured pork loin added with FSC was decreased with increasing FSC level. Residual nitrite level and shear force were increased with increasing FSC level. In the sensory evaluation, sensory score for flavor, off-flavor, chewiness, juiciness, and overall acceptability were not significantly different among all treatments. However, sensory score for color was increased when the concentration of FSC added to pork loin was increased. Conclusion: The FSC solution had a positive effect on redness and lipid oxidation. As shown by the results in protein deterioration and sensory, Swiss chard can replace sodium nitrite as natural curing agent.

Amperometric Determination of Nitrite at Poly(Methylene Blue)-Modified Glassy Carbon Electrode

  • Xu, Guang-Ri;Xu, Guifang;Xu, Ming-Lu;Zhang, Zhengqing;Tian, Yuan;Choi, Han-Nim;Lee, Won-Yong
    • Bulletin of the Korean Chemical Society
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    • v.33 no.2
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    • pp.415-419
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    • 2012
  • Electrochemical characteristics of nitrite ion were investigated at a poly(methylene blue)-modified glassy carbon electrode by cyclic voltammetry and differential pulse voltammetry. The poly(methylene blue)-modified glassy carbon electrode exhibited enhanced anodic signals for nitrite. The effects of key parameters on the detection of nitrite were evaluated at the modified electrode, such as pH, accumulation time, and scan rate. Under optimum condition, the chemically modified electrode can detect nitrite in the concentration range $2.0{\times}10^{-6}$ to $5.0{\times}10^{-4}$ M with the detection limit of $2.0{\times}10^{-6}$ M and a correlation coefficient of 0.999. The detection of nitrite using the chemically modified electrode was not affected by common ions such as $Na^+$, $K^+$, $Ca^{2+}$, $Cl^-$, $HPO_4^{2-}$ and $H_2PO_4^- $. The modified electrode showed good stability and reproducibility. The practical application of the present method was successfully applied to the determination of nitrite ion in cabbage samples.

Autotrophic Nitrite Denitrification Using Sulfur Particles for Treatment of Wastewaters with Low C/N Ratios (Batch Tests) (C/N비가 낮은 하.폐수에서 황입자를 이용한 아질산성질소 탈질 연구(회분식 실험))

  • Yoon, Seung-Joon;Kang, Woo-Chang;Bae, Woo-Keun;Oh, Sang-Eun
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.9
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    • pp.851-856
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    • 2010
  • A sulfur utilizing nitrite denitrification process could be placed after the shortcut biological nitrogen removal (SBNR) process. In this study, removal of nitrite using sulfur oxidizing denitrifier was characterized in batch tests with granular elemental sulfur as an electron donor and nitrite as an electro acceptor. At sufficient alkalinity, initial nitrite nitrogen concentration of 100 mg/L was almost completely reduced in the batch reactor within a incubation time of 22 h. Sulfate production with nitrite was 4.8 g ${SO_4}^{2-}/g$ ${NO_2}^-$-N, while with nitrate 13.5 g ${SO_4}^{2-}/g$ ${NO_3}^-$-N. Under the conditions of low alkalinity, nitrite removal was over 95% but 15 h of a lag phase was shown. For nitrate with low alkalinity, no denitrification occurred. Sulfate production was 2.6 g ${SO_4}^{2-}/g$ ${NO_2}^-$-N and alkalinity consumption was 1.2 g $CaCO_3/g$ ${NO_2}^-$. The concentration range of organics used in this experiment did not inhibit autotrophic denitrification at both low and high alkalinity. This kind of method may solve the problems of autotrophic nitrate denitrification, i.e. high sulfate production and alkalinity deficiency, to some extent.

Phosphorus Removal by DPAOs (Denitrifying Phosphorus Accumulating Organisms) in Aerobic Condition (호기 조건에서 DPAOs (Denitrifying Phosphorus Accumulation Organisms)에 의한 인 제거)

  • Jeong, No-Sung;Park, Young-Seek;Kim, Dong-Seog
    • KSBB Journal
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    • v.25 no.1
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    • pp.62-66
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    • 2010
  • This study was carried out to get phosphorus uptake rate in aerobic condition with nitrate and nitrite. Nitrate and nitrite inhibited phosphorus accumulating organisms' (PAOs') luxury uptake in aerobic condition. Nitrite awfully decreased the phosphorus uptake rate in aerobic condition. At the influent of 10 mg ${NO_3}^-$-NL, the phosphorus uptake was decreased to 52% comparing that at no influent of nitrate. And at the influent of 10 mg ${NO_2}^-$-NL, the phosphorus uptake was decreased to 28% comparing that at no influent of nitrite. At the influent of 20 mg ${NO_3}^-$-NL, nitrite and nitrate were co-existed and the phosphorus uptake rate was decreased to 16% comparing that at no influent of nitrite and nitrate. Also, the denitrification was occurred by denitrifying glycogen accumulating organisms (DGAOs)/denitrifying phosphorus accumulating organisms (OPAOs) in spite of aerobic condition, and the phosphorus uptake rate was increased by the decrease of influent nitrate concentration at the aerobic condition. The inflection point in the phosphorus uptake rate was shown at the nitrite concentration of 1.5~2 mg/L.