Parasites, Hosts and Diseases
- Volume 33 Issue 4
- /
- Pages.349-356
- /
- 1995
- /
- 2982-5164(pISSN)
- /
- 1738-0006(eISSN)
Effect of sodium nitrite on Trichomonas vaginalis
아질산나트륨(sodium nitrite)이 질편모충 증식과 미세구조에 미치는 영향
- Ryu, Jae-Suk (Department of Parasitology, College of Medicine, Hanyang University) ;
- Buk, Jung-Hwa (Department of Parasitology, College of Medicine, Hanyang University) ;
- Min, Deuk-Yeong (Department of Parasitology, College of Medicine, Hanyang University)
- Published : 1995.12.01
Abstract
We have investigated the action of sodium nitrite on the growth and morphologic changes of T uaginolis and on the treatment of subcutaneous abscess by trichomonad in mice. Sodium nitrite inhibited the growth of metronidazole-sensitive KT9 isolate and metronidazole-resistant CDC85 strain of T vcsinalis as concentration of 6 mM and 10 mM respectively Intraperitoneal injection of sodium nitrite (70 ㎍, 100 ㎍, 130 ㎍/g body weight) did not reduce the size of abscess produced by subcutaneous inoculation of T uasinnlis in mice. T uosinnlis, treated with sodium nitrite at concentration giving about 50% inhibition of growth, showed fissures, many vacuoles and electron-translucent zone in the cytoplasm by transmission electron microscopy. In the case of CDC85 treated with 9 mM sodium nitrite, hydrogenosomal matrical change , destruction of hydrogenosomal membrane, autophagic vacuoles, disappearance of Golgi complex and polysome were notably observed . With above results, it is assumed that sodium nitrite inhibits the growth of metronidazole-sensitive and - resistant strains of T. ucsinalis and induces the morphological changes of 7 uusinalis although it does not affect in reducing of abscess size by vagiginalis in mice.
이 연구에서는 방부제로 사용되는 아질산나트륨이 질편모충에 어떤 영향을 주는지 알 아보고자 in vivo 및 in vitro에서 관찰하였다 Metronidasole 감수성인 KT9 분리주 및 내성인 CDC85주의 배양배지에 아질산나트륨을 넣은 경우 10% 이하로 증식이 억제되는 농도는 KT9주에서는 6mM, CDC85주에서는 10 mM로 KT9 분리주가 CDC85주에 비해 아질간나트륨의 더 낮은 농도에서 증식이 억제되었다. KT9 분리주 및 CDC85주를 마우스 피하에 주사하고 마우스 체중 g당
Keywords
- Trichomonas vaginalis;
- metronidazole-sensitive KT9 isolate;
- metronidazole-resistant CDC85 strain;
- sodium nitrite