• The Korea Journal of Herbology
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• v.34 no.1
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• pp.109-116
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• 2019

Objectives : This study aimed to analyze contents of chlorogenic acid among major ingredients and determine the optimal solvent for the antioxidant activity from Solidago virga-aurea var. gigantea(SV) aerial part. Methods : Mature aerial part of extracted SV at the first crop on June 2018 was used. SV was mixed with each at the ethanol concentrate rates of water, 10, 30, 50, 70, and 100% and extracted them for three times for eight hours at $70^{\circ}C$. The contents of chlorogenic acid and seven kinds of antioxidant activities were measured in SV extracts. Results : The highest the contents of chlorogenic acid was from 10% ethanol extracts showed. Total polyphenol and flavonoids of SV extracts were 126.16 mg/g and 105.84 mg/g, respectively. Scavenging activities of DPPH, ABTS, and hydroxyl radical significantly increased in a dose-dependent in SV extracts, while it was almost similar with control at the concentration of $500{\mu}g/mL$. $Fe^{2+}$ chelating activity significantly increase in a dose-dependent in SV extract, it was lower than control. Nitrite scavenging activity increased, dose-dependent in the sequence of pH 1.2> pH 3.0 > pH 6.0 in SV extracts, while it was almost similar with control at the concentration of $500{\mu}g/mL$, pH 1.2, and pH 3.0. Conclusions : Thus, this study found that higher contents of chlorogenic acid and excellent antioxidant activity were contained in 10% ethanol extracts in mature aerial part of SV. It is expected to be used as basic data as natural antioxidant materials.

• KSBB Journal
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• v.26 no.2
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• pp.112-118
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• 2011

Contents of amino acids, vitamins, and minerals as well as its antioxidant activitiy of Laminaria japonica water extract have been analyzed for preparation of functional foods and cosmetic products. From the analysis of total amino acids, eighteen kinds of amino acids were found in the water extract of Laminaria japonica. Among total amino acids, the order of contents was glutamic acid (2.07 mg/g), alanine (0.51 mg/g), aspartic acid (0.44 mg/g), glycine (0.34 mg/g), and valine (0.34 mg/g). In case of free amino acids, glutamic acid (0.95 mg/g), prolin (0.54 mg/g), aspartic acid (0.44 mg/g), leucine (0.07 mg/g), and phenylalanine (0.07 mg/g) were dominant compositions. Vitamin E was only detected in water extract of Laminaria japonica. The mineral contents were as follows: K 752.60 mg, Na 259.20 mg, Ca 80.20 mg, P 29.50 mg, and Fe 8.32 mg based on 100 g Laminaria japonica water extract. The nitrite scavenging activity of the extract were gradually increased with the extracts contents to 86.2% at concentration of 100 mg/mL and DPPH radical scavenging activity of the extracts were 86.4% at concentration of 50 mg/mL.

• Korean journal of applied entomology
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• v.15 no.4 s.29
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• pp.205-214
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• 1976

Effect of Butachlor(2-chloro-2, 6-diethyl N-(buthoxymethyl) acetanilide), Nitrofen(2,4-dichloro-4-nitrodiphenyl ether), Benthiocarb+Simetryne(s-(4-chlorobenzyl)-N, N-diethylthiocarbamate $7\%$+2-methylthio-4, 6-bis(ethylamino)-s-triazine $1.5\%)$, Propanil (3,4-dichloropropionanilide), and Perfluidone {1. 1. 1-trifluoro-N, N-(2-methyl-4-(phenylsulfonyl) Pheny1) methanesulfon amide} on urea hydrolysis and subsequent nitrification was investigated in a flooded soil incubated at $24\pm1^{\circ}C$ for 9 weeks. 1. Butachlor and Perfluidone at the rate of 1,440 and 1,200g, ai/10a, respectively, slightly inhibited the early stage of urea decomposition, and caused a slight decrease in the production of ammomium, which, however, was recovered readily. 2. Propanil at the rate of 2,800g, ai/10a imhibited the first stage of nitrification, and brought about a slight increase in the ammonium conentration and a decrease in the concentration of nitrite and nitrate. This inhibitive effect was a little more evident at higher concentration of applied nitrogen. The other herbicides caused no inhibition of urea decomposition and subsequent nitrification even at the highest rate of application. 3. pH and Eh of the soil were not significantly affected by the herbicides tested.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.9
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• pp.1204-1210
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• 2011

Objective of this study was to examine the effect of sodium nitroprusside (SNP), a nitric oxide (NO) donor on steroid synthesis, growth and apoptosis of buffalo granulosa cells (GCs) in vitro. Follicular fluid of antral follicles (3-5 mm diameter) was aspirated and GCs were cultured in 0 (control), $10^{-3}$, $10^{-5}$, $10^{-7}$, $10^{-9}\;M$ of SNP for 48 h. To evaluate whether this effect was reversible, GCs were cultured in presence of $10^{-5}\;M$ SNP+1.0 mM $N^{\omega}$-nitro-L-arginine methyl ester (L-NAME) a NO synthase (NOS) inhibitor or hemoglobin (Hb, $1.0{\mu}g$) as NO scavenger. Nitrate/nitrite concentration was evaluated by Griess method, progesterone and estradiol concentrations by RIA and apoptosis by TUNEL assay. SNP ($10^{-3}$, $10^{-5}$, $10^{-7}\;M$) significantly (p<0.05) inhibited estradiol and progesterone synthesis, growth, disorganized GCs aggregates and induced apoptosis in a dose dependent manner. However, $10^{-9}\;M$ SNP induced the progesterone synthesis and stimulated GCs to develop into a uniform monolayer. Combination of SNP $10^{-5}$ M+L-NAME strengthened the inhibitory effect while, SNP+Hb together reversed these inhibitory effects. In conclusion, SNP at greater concentrations ($10^{-3}$, $10^{-5}$ and $10^{-7}\;M$) has a cytotoxic effect and it may lead to cell death whereas, at a lower concentration ($10^{-9}\;M$) induced progesterone synthesis and growth of GCs. These findings have important implications that NOS derived NO are involved at physiological level during growth and development of buffalo GCs which regulates the steroidogenesis, growth and apoptosis.

• Journal of Microbiology and Biotechnology
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• v.13 no.2
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• pp.207-216
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• 2003

A number of different sources, such as composts, leachates, and pig feces samples, were collected from different pig farms in Korea, and several microorganisms were screened for their ability to deodorize the malodorous gases. Consequently, a novel malodorous gases-deodorizing bacterial strain, KJ-108. was isolated, because it was highly abundant in nitrate-supplemented minimal medium ($MM-NO_3^-$) under anaerobic culture conditions. Airtight crimp-sealed serum bottles containing $MM-NO_3^-$ , medium were inoculated with KJ-108. Nitrate concentration was decreased rapidly after 20 h of incubation, and incubation was carried out until nitrite production reached almost zero. Taxonomic identification, including 16S rDNA base sequencing and phylogenetic analysis, indicated that the isolate had $100\%$ homology in its 165 rDNA base sequence with Lactobacillus pentosus. Among the volatile fatty acids, acetic acid contained in large amounts in fresh piggery slurry was decreased by about $40\%$ after 50 h incubation with strain KJ-108. n-Butyric acid, n-valeric acid, and isovaleric acid were gradually decreased, and isobutyric acid and capronic acid were dramatically eliminated at theinitial period with the treatment. Moreover, NH, removal efficiency reached a maximum of $98.5\%$ after 50 h of incubation, but the concentration of $H_2S$ was not changed.

• Journal of Korean Society of Water and Wastewater
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• v.23 no.5
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• pp.547-555
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• 2009

The objectives of this research were focused on the effects of various operating parameters on nitrous oxide emission such as C/N ratio, ammonia concentration and HRT in the hybrid and suspension reactors. With the decreasing of C/N ratios, $N_2O$ emission rates in the both processes were increased because organic carbon source for denitrification was depleted. In case of biofilm reactor operated using medium, $N_2O$ release from the nitrification was not affected by the variation of ammonia concentration. But in the suspension reactor, $N_2O$ production from the nitrification was rapidly increased with the increase of ammonia. Nitrite accumulation caused by undesirable nitrification conditions could be a important reason for the increase in the $N_2O$ production from the aerobic reactor. And rapid increase in $N_2O$ production was reflected by the decrease of HRT, similar to the results observed in the results of ammonia loading changes. So it could be said that it is very important to put in consideration both its optimum conditions for wastewater treatment efficiency and suitable conditions for $N_2O$ diminish, simultaneously, in order to development an eco-friendly and advanced wastewater treatment, especially in BNR process.

• The Korean Journal of Physiology and Pharmacology
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• v.3 no.4
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• pp.447-454
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• 1999

The production of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) are known to be modulated by a variety of factors. Recent study showed that endotoxin-induced NO synthesis and iNOS expression were greatly enhanced by elevation of extracellular glucose concentration in murine macrophages. Although this was suggested to be due to the activation of protein kinase C (PKC) via sorbitol pathway, there was lack of evidence for this speculation. This study was performed to delineate the underlying intracellular mechanisms of glucose-enhancing effect on endotoxin-induced NO production in Raw264.7 macrophages. The levels of NO release induced by lipopolysaccharide (LPS) significantly increased by the treatment of glucose in a concentration dependent manner and also, this effect was observed in LPS-preprimed cells. Concurrent incubation of cells with PKC inhibitors, H-7 or chelerythrine, and LPS resulted in the diminution of NO production regardless of glucose concentration but this was not in the case of LPS-prepriming, that is, chelerythrine showed a minimal effect on the glucose- enhancing effect. PMA, a PKC activator, did not show any significant effect on glucose-associated NO production. Modulation of sorbitol pathway with zopolrestat, an aldose reductase inhibitor, did not affect LPS-induced NO production and iNOS expression under high glucose condition. And also, sodium pyruvate, which is expected to normalize cytosolic $NADH/NAD^+$ ratio, did not show any significant effect at concentrations of up to 10 mM. Glucosamine marginally increased the endotoxin-induced nitrite release in both control and high glucose treated group. 6-diazo-5-oxonorleucine (L-DON) and azaserine, glutamine: fructose- 6-phosphate amidotransferase (GFAT) inhibitors, significantly diminished the augmentation effect of high glucose on endotoxin-induced NO production. On the other hand, negative modulation of GFAT inhibitors was not reversed by the treatment of glucosamine, suggesting the minimal involvement, if any, of glucosamine pathway in glucose-enhancing effect. In summary, these results strongly suggest that the hexosamine biosynthesis pathway and the activation of PKC via sorbitol pathway do not contribute to the augmenting effect of high glucose on endotoxin induced NO production in macrophage-like Raw264.7 cells.

• Food Engineering Progress
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• v.13 no.1
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• pp.16-23
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• 2009

Onion juices supplemented with different concentrations of puffed red ginseng extract were fermented using Pediococcus pentosaceus KC-007 and their biologically functional properties were investigated. When onion juices were supplemented with puffed red ginseng extract at the concentration of 0.5, 1, 2, and 4% (v/v) each, viable cell number of lactic acid bacteria was the highest at 24 hr of fermentation in all samples. The titratable acidity increased as the fermentation proceeds irrespective of the added amount of red ginseng extract, and the pH of fermentation broth decreased until 36 hr of fermentation. The reducing sugar of fermentation broth decreased until 24 hr of fermentation and did not change thereafter. The electron donating ability and nitrite scavenging ability were highest when red ginseng extract was added at the concentration of more than 1% (w/v). The overall acceptance in sensory evaluation was the best when red ginseng extract was added at the concentration of 1% (w/v). From these results, it is confirmed that the optimum concentration of puffed red ginseng extract for the lactic acid fermentation of onion juice was 1% (w/v).

• Proceedings of the Korean Environmental Health Society Conference
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• 2001.11a
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• pp.19-29
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• 2001

Nutrients are key environmental factors in marine ecosystem. They limit algal growth when at low concentrations and cause algal bloom when at high contents. They also control the growth and succession of many other biota including bacteria and zooplankton, either directly or indirectly. Nutrient contents therefore affect both the structure and functions of marine ecosystem. To study the contents and distribution of nutrients in Chinese Bohai Sea, two cruise surveys were undertaken in August 2000 (summer) and January 2001 (winter), respectively. A total of 595 water samples were collected from 91 sites. After collection the samples were transported to the laboratory and five nutrients, i.e., nitrate, nitrite, ammonia, phosphate and silicate, were analyzed. The results showed that tile average concentration of total inorganic nitrogen (TIN) in Bohai Sea in winter (6.5293.717 ${\mu}$mol$.$l$\^$-1/) was significantly higher than that in summer (3.717 ${\mu}$mol$.$l$\^$-1/). The phosphorus concentration in winter (0.660 ${\mu}$mol$.$l$\^$-1/) was also significantly higher than that in summer (0.329 ${\mu}$mol$.$l$\^$-1/). Mean silicate concentration in winter (7.858 ${\mu}$mol$.$l$\^$-1/) was not significantly different from that in summer (7.200 ${\mu}$mol$.$l$\^$-1/). Nutrients also varied considerable among different areas within Bohai Sea. TIN concentration in Laizhou Bay (4.444 ${\mu}$mol$.$l$\^$-1/), for example, was significantly higher than those in Bohai Bay (2.270 ${\mu}$mol$.$l$\^$-1/) and Bohai Straight (2.431 ${\mu}$mol$.$l$\^$-1/), which probably reflects tile discharge of large amounts of nitrogen into Laizhou Bay via Yellow River. The nutrients also showed vertical distribution pattern. In summer, nutrients in bottom layer were generally higher than those in surface and medium layers. In winter, however. nutrients in different layers were not significantly different Compared with historic data, TIN contents increased continuously since early 1980s, phosphorus arid silicone contents, nevertheless, fell down to some degree. Based on atomic ratios of different nutrients, nitrogen is still the main limiting factor for algal growth in Bohai Sea.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.8
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• pp.1062-1069
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• 2007

Response surface methodology was employed to optimize extraction conditions for finding the maximizing the functional properties of roasted Pleurotus eryngii. Based on the central composite design, independent variables were ethanol concentration ($0{\sim}100%$), extraction time ($1{\sim}9$ min) and microwave power ($25{\sim}125$ W). Soluble solid content, electron donating ability and nitrite-scavenging ability were mainly affected by ethanol concentration, but ACE inhibition activity was largely affected by extraction time. The optimum ranges of extraction conditions resulting from superimposing the response surface were predicted to be ethanol concentration ($25{\sim}50%$), extraction time ($3{\sim}9$ min) and microwave power ($80{\sim}125$ W). Total protein and total phenolic compound content of optimal extracts were 45.80 mg/g and 7.42 mg/g, respectively. In phenolic compounds of roasted Pleurotus eryngii extracts, protocatechuic acid was the highest concentration at 1226.32 ${\mu}g/g$, followed by salicylic acid, catechin, p-hydroxybenzoic acid, caffeic acid, coumaric acid and hesperidin.