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http://dx.doi.org/10.3746/jkfn.2007.36.8.1062

Optimization of Extraction Conditions for Functional Components of Roasted Pleurotus eryngii by Microwave-Assisted Extraction  

Lee, Myung-Hee (Dept. of Bio-Food, Kyongbuk College of Science)
Yoon, Sung-Ran (Traditional Food Institute, Kyongbuk College of Science)
Jo, Deok-Jo (Traditional Food Institute, Kyongbuk College of Science)
Kim, Hyun-Ku (Korea Food Research Institute)
Lee, Gee-Dong (Bio Industry Center, Daegu New Technology Agency)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.36, no.8, 2007 , pp. 1062-1069 More about this Journal
Abstract
Response surface methodology was employed to optimize extraction conditions for finding the maximizing the functional properties of roasted Pleurotus eryngii. Based on the central composite design, independent variables were ethanol concentration ($0{\sim}100%$), extraction time ($1{\sim}9$ min) and microwave power ($25{\sim}125$ W). Soluble solid content, electron donating ability and nitrite-scavenging ability were mainly affected by ethanol concentration, but ACE inhibition activity was largely affected by extraction time. The optimum ranges of extraction conditions resulting from superimposing the response surface were predicted to be ethanol concentration ($25{\sim}50%$), extraction time ($3{\sim}9$ min) and microwave power ($80{\sim}125$ W). Total protein and total phenolic compound content of optimal extracts were 45.80 mg/g and 7.42 mg/g, respectively. In phenolic compounds of roasted Pleurotus eryngii extracts, protocatechuic acid was the highest concentration at 1226.32 ${\mu}g/g$, followed by salicylic acid, catechin, p-hydroxybenzoic acid, caffeic acid, coumaric acid and hesperidin.
Keywords
microwave-assisted extraction; Pleurotus eryngii; optimization; electron donating ability; ACE inhibition activity;
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Times Cited By KSCI : 12  (Citation Analysis)
Times Cited By SCOPUS : 2
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