• Food Science and Preservation
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• v.22 no.6
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• pp.831-837
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• 2015

Insects have gained increasing attention as an alternative protein and nutrient rich food source for humans. This study was conducted to investigate the physicochemical characteristics and harmful components of edible crickets (Gryllus bimaculatus) in the 6 districts of Yeonggwang (YG), Jeongseon (JS), Wonju (WJ), Hwaseong (HS), Geochang (GC), and Chungju (CJ). The average crude protein and crude lipid contents on a dry basis were 64.34% and 16.60%, respectively. The crude protein content of CJ was the highest (67.40%), whereas YG (59.42%) had the lowest content. On the other hand, the crude fat content of YG was the highest (20.61%), whereas CJ (14.04%) had the lowest content. The unsaturated fatty acid contents were 57.97-63.93 g/100 g of the total fatty acid content in the crickets of the 6 districts. The major fatty acids of the crickets in the 6 districts were palmitic acid, oleic acid, and linoleic acid. Among the essential amino acids, valine, leucine, and lysine were the most abundant. GC had the highest total amino acids (57.93 g/100 g), whereas YG (48.65 g/100 g) had the lowest. Major mineral contents included potassium (K, 0.92~1.01 mg/100 mg) and phosphorus (P, 0.74~0.88 mg/100 mg). The mineral composition was fairly similar among the crickets. Crickets in the 6 districts were verified to have safe levels of residual heavy metals according to the Korea Food & Drug Administration (KFDA) advisory levels.

• Journal of Applied Biological Chemistry
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• v.52 no.1
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• pp.8-14
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• 2009

This study was conducted to assess the effects of diet food containing Jerusalem artichoke's inulin, lotus leaf, and herb on weight and body fat. Participants in this study were selected based on the following criteria: BMI over $25kg/m^2$, body fat percentage higher than 25%, abdominal obesity level of 0.85 measured by body composition measurement unit (ZEUS 9.9 PLUS, Korea) total 24 female and male university students over 20 of age were assigned to two different groups: control group and diet group and the study was carried out for 30 days. When we measured what the tester's body weight and height, we used an automatic measure machin which is called IMI-1000 from Immanuel company and when we measured what the hip and wist circumference, we used a tape measure. When we measured body mss index (BMI), body Int, body mass, lean mass, waist/hip ratio (WHB), obesity index, we used a ZEUS9.9 PLUS-Korea which is based on bioelectrical impedance analysis, The food intake was checked by means of diet record method to be input into CAN program in order to analyze nutrient intake. Our findings indicated that the diet group, as compared to the control group, lost weight of approximately 2.5 kg and showed statistically significant difference. In addition the level of body Int, muscle, abdominal obesity, obesity, waist and bottom measurement all showed significant decrease after study period. However, there was no big difference in body fat percentage because both body fat level and muscle level dropped together, Putting all these together, diet food in this study containing Jerusalem artichoke's inulin, lotus leaf powder, and herbs powder including nettle, eucalyptus was found to be effective in significant reduction of weight and body fat and obesity-related body indicators. Also, it is considered that this diet food has potential to prevent and improve effectively obesity from abnormal fat accumulation.

• Korean Journal of Organic Agriculture
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• v.14 no.1
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• pp.55-67
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• 2006

A survey of 31 organic farmers were conducted to investigate the actual conditions of organic matter application. The amounts of organic matter application in the fields were higher in order of fruit, vegetable and rice farm. The average was 50 ton/ha in vegetable farms. In the green vegetable farms saw dust and animal manure were mainly utilized to make compost. Rice straw, wood chip, and forest bushes were also used for composting. In the fruit vegetable farms materials relatively lower in nitrogen content such as rice straw and cattle manure were used in vegetative period and materials higher in nitrogen content such as oil cake and wild grass were used in reproductive phase. Nutrient balance investigated in the farm in Icheon region who produce lettuce, angelica, and kale continuously in one cropping year indicated surplus in three major nutrients. Nitrogen and phosphorous were in excess by 29 and 10 kg respectively in the organic rice farm in yang-pyoung region. While soil chemical properties in the organic farms are within the adequate range in open field, it is much higher than the limits in the greenhouse soils. Overall application of organic matter is in an oversupply state. This results suggested that the organic matter management should be based on the soil conditions for sustainable cultivation. Chemical composition of organic matters and soil test reports should be considered prior to the application of organic matter.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.1
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• pp.45-51
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• 2013

This study was performed on two groups (10 cows) for primiparous Holstein lactating cows (av. 98 days in milk : DIM) which were divided into low forage diet (LF) and high forage diet (HF) groups based on forage : concentrate ratio (F : C ratio). The F : C ratios of LF and HF groups were 37:63 and 62:38, respectively. Concentrate intake was significantly higher in the LF group than the HF group whereas the HF group showed higher forage intake (12.9 kg) compared to the LF group (7.4 kg) (p<0.05). No significant difference was observed in total feed intake between the HF (20.9 kg) and LF (19.4 kg) group (p>0.05), but the HF group tended to be higher. CP, TDN and NEL intake showed no significant difference between the two groups (p>0.05). Though, there was no significant difference on actual milk between the two groups (26 vs. 23.9 kg/d, p>0.05), the LF group tended to be higher. 4% FCMs of LF and HF groups were 22.8 and 22.3 kg/d, respectively, and showed no significant difference (p>0.05). The HF group was higher in fat content and lower in MUN. C14:0, C16:0 and C16:1n7 of milk fatty acid were significantly higher in the HF group (p<0.05), but there were no differences in other milk fatty acids between the two groups. As a result, increasing high quality forage such as BIRG silage and hay in the diet will not only fulfill nutrient requirements but also reduce milk production.

• 한국해양학회지
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• v.27 no.3
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• pp.250-258
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• 1992

The oxygen isotope composition of surface waters in the Bransfield Strait was determined as one extra state variable in order to characterize water masses in the region, since salinity is significantly modified due to the freezing and ice-melting in the polar region. The salinity, temperature, and $\delta$/SUP 18/O values vary from 34.0 to 34.5$\textperthousand$, -.05 to 2.1$^{\circ}C$ and -0.50 t -0.26$\textperthousand$, respectively. The combined effects of evaporation, precipitation, freezing, ice-melting are reflected in the widely scattered data. Although it is small, the distribution of $\delta$/SUP 18/O of the Bransfield Strait is strongly affected by the freezing-ice melting rather than the evaporation-precipitation. The ice melted fresh water which has higher temperature, depleted salinity and nutrients may be injected to the Bransfield Strait from the north. The concentrations of nutrients are decreasing gradually from the north to the south. The waters were characterized by two groups of higher (about 19.4) and lower N/P ratio (about 16.7). The lower N/P ratio is found in the northern part where ice-melted fresh water is injected. and the higher N/P ratio is found in the southern part of the Bransfield Strait. Although more precise work is needed, the deference of N/P ratio can be an evidence of the ice melted water injection to the Bransfield Strait. Chlorophyll a concentrations, in general, increase from northwest (Waddell Sea) to the southeast (Smith and Hosseason Islands). Probably the injection of nutrient depleted fresh water from the ice melting reduce the chlorophyll a concentration.

• Journal of Aquaculture
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• v.6 no.1
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• pp.29-46
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• 1993

In order to determine the optimum digestible energy to protein (DE/P) ratio in diets for Korean rockfish, Sebastes schlegeli, $45\%$ and $40\%$ protein diets with graded levels of DE/P ratio were fed to average 36g and 80g fish, respectively. The DE/P ratios in the $45\%$ protein diets ranged from 7.4 to 10.0 kcal/g protein, while those in the $40\%$ protein diets varied between 7.5 and 8.9 kcal/g protein. Both in the $45\%$ and $40\%$ protein diets, the changes in the DE/P ratios did not affect either the daily weight gain or the feed efficiency. However daily protein retention, daily energy retention, nutrient utilization and chemical composition in carcass varied depending on the DE/P ratios. Thus based on these parameters as criteria, the optimum DE/P ratio for both the $40\%$ and $45\%$ protein diets, estimated to be about 8 kcal/g protein. DE/P ratios more than 8 kcal/g protein, led to the deposition of a large amount of body fat, especially in the viscera. Any protein-sparing effect was not observed with the increase of the energy content in the diets. Apparent digestibility coefficients determined by the indirect method, using $Cr_2O_3$ as an indicator, were around $90,\;98\%\;and\;70\%$, for protein, lipid and digestible carbohydrate respectively, without any distinct differences between the diets.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.143-144
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• 2003

봄철 해역에 따른 부착미세조류의 영양성분 변화를 알아보기 위하여 본 실험을 수행하였다. 실험은 울진, 부안, 완도, 부산 지역에서 봄철인 2~4월 동안 실시되었으며 실험간의 오차를 줄이기 위해 실험 시작일의 차이는 최대 10일 이내로 하였다. 배양을 위한 실험해역의 기초 조사로 배양지역 연안해수의 수온, 염분, pH, 총질소, 총인, Chlorophyll-a의 측정을 하였고 각 지역의 부착미세조류를 수확하여 영양분석을 하였다. 실험에 사용된 파판은 전복 종묘생산에 이용되는 규조배양용 파판과 틀을 이용하였고 실험기간은 총 8주 동안 실시하였다. 2주마다 파판을 수거한 뒤 부착된 미세조류를 부드러운 솔로 분리하여 원심 분리한 후 지방산과 아미노산의 측정을 하였다. 또 각 시료의 일정량을 Sedwick-rafter counting chamber를 이용하여 검경ㆍ계수한 후 이를 단위면적당 개체수(cells/$\textrm{cm}^2$)로 환산하여 biomass를 구하였다. 봄철 지역간의 수온의 범위는 13.9~16.6$^{\circ}C$를 보였으며 울진이 가장 높고 부안지역이 가장 낮았다. 염분은 부안지역의 염분이 평균 2$\textperthousand$ 이상 낮았으며 그 외의 지역은 큰 차이가 없었다. pH는 7.77~8.21의 범위를 보이고 있었으며 질산염 농토는 부산이 0.265mg/$\ell$로 최대값을 보였고 울진이 0.032mg/$\ell$로 제일 낮았다. 인산염 농도는 완도지역이 0.018mg/$\ell$로 최대치를 보였고 부안은 검출되지 않았다. Chlorophyll-a의 농도는 울진지역이 0.66mg/$\ell$으로 제일 낮았으며 부산이 2.69mg/$\ell$, 부안지역이 2.27mg/$\ell$ 로 높은 값을 보였다. 봄철 지역에 따른 부착미세조류의 아미노산 분석결과는 울진이 실험지역 중 제일 높은 총 아미노산과 필수아미노산의 함량을 나타냈으며 각 지역별로 울진은 4주에 20.3%, 부안은 8주째 8.1%, 완도는 8주째 4.7%, 부산은 6주째 10.4%로 서로다른 시기에 높은 아미노산의 함량을 나타냈다. 실험지역 부착미세조류의 지방산 결과를 보면 전 실험기간동안 지방산 중 대부분은 $C_{18}$이하의 포화 또는 불포화 지방산이 주를 이루고 있었으며 palmitic acid( $C_{16:0}$)와 palmitoleic acid( $C_{16:0}$)의 양이 전체 지방산 함량중 37.47~75.94%로 대부분을 차지하고 있었다. 그리고 n-3HUFA(high unsaturated fat쇼 acids)인 EPA는 부안지역이 2주째 20.85%로 전체 지역중 함량이 제일 높았고, 울진은 실험기간중 6주가 14.76%로 제일 높은 함량을 보였다. 완도는 6주에만 10.97%의 함량을 보였으며 부산지역의 시료에서는 EPA의 검출이 없었다. 봄철 각지역에 설치된 파판에서의 부착미세조류 출현량을 보면 2주째의 부착미세조류량은 부안이 8.3$\times$$10^3$ cells/$\textrm{cm}^2$로 가장 높았고 부산이 0.6$\times$$10^3$ cells/$\textrm{cm}^2$로 가장 낮았다. 울진의 경우 0.7$\times$$10^3$ cells/$\textrm{cm}^2$로 부산과 비슷하게 낮은 출현량을 보였고 완도는 2.4$\times$$10^3$ cells/$\textrm{cm}^2$였다. 4주째의 출현량은 울진지역이 471.1$\times$$10^3$ cells/$\textrm{cm}^2$로 가장 높았으며 부안, 완도, 부산 순으로 출현량이 낮았고 특히 부산은 1.2$\times$$10^3$ cells/$\textrm{cm}^2$로 가장 낮았다. 6주째의 출현량은 울진 지역이 368.6$\times$$10^3$ cells/$\textrm{cm}^2$로 가장 높았으며 부안 89.8$\times$$10^3$ cells/$\textrm{cm}^2$, 완도 25.1$\times$$10^3$ cells/$\textrm{cm}^2$, 부산 5.9$\times$$10^3$ cells/$\textrm{cm}^2$ 순이었다. 8주째의 경우에도 울진, 부안, 완도, 부산이 각각 255.8$\times$$10^3$ cells/$\textrm{cm}^2$, 157.8$\times$$10^3$ cells/$\textrm{cm}^2$, 44.3$\times$$10^3$ cells/$\textrm{cm}^2$, 1.1$\times$$10^3$ cells/$\textrm{cm}^2$로 4주, 6주의 경우와 같은 경향을 보였다.보였다.

• Journal of Life Science
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• v.12 no.6
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• pp.700-707
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• 2002

To study the characteristics of organic and nutrient removal by Bacillus species at high COD concentration of influent, three lab-scale batch reactors(R1, R2, R3), each of which has different substrate composition, were operated. More than 95% of $NH_4^+$-N and $COD_{cr}$, concentrations were removed under an aerobic condition, and their removal efficiencies were found to be 22.6 and 90.5%(R1), 23.9 and 65.8%(R2), 30.2 and 86.4%(R3), respectively. The removal efficiency of $NH_4^+$-N was high when an enough amount of $NO_3^{-}$-N was supplied, and that of $COD_{cr}$. was low when a high concentration of initial $NO_2^{-}$-N was added. The amount of carbon utilized in denitrification was a little. In all reactors,$NO_3^{-}$-N was removed under an anoxic condition, but in the R3 reactor, 10% of $NO_3^{-}$-N could be removed even undo, an aerobic condition. The removal efficiencies of TN and TP were 41.8 and 49.5%(R1), 40.1 and 35.8%(R2), 47.0 and 57.6%(R3), respectively. Alkalinities destructed under an aerobic condition for each reactor were 4.96, 5.41 and 3.93 mg/L (as $CaCO_3$) per each gram of $NH_4^+$-N oxidized, respectively, while 3.06, 3.17 and 2.60 mg/L (as $CaCO_3$) of alkalinities were produced for each gram of ,$NO_3^{-}$-N reduced to $N_2$. The SOUR were found to be 38.5, 52.7 and 42.0 mg $O_2$/g MLSS/hr, which indicated that Bacillus sp. had a higher cell activity than activated sludge. The OLR and sludge production were estimated to be 0.69 and 0.28(Rl), 0.77 and 0.20(R2), 0.61 kg COD/$m^3$/day and 0.25 kg MLSS/kg COD(R3), respectively. From the N-balance, the highest percentage(40.9%) of nitrogen lost to $N_2$ was obtained in the R3 reactor. From all the results, the possibility of aerobic denitrification Bacillus sp. has been shown and the B3 process seemed to have two advantages: a little amount of carbon was required in denitrification and not much amount of alkalinity was destructed under an aerobic condition.