• 제목/요약/키워드: component protein

Search Result 1,198, Processing Time 0.034 seconds

Nutritive Components of Edible Bamboo Shoots of Phyllostachys edulis Produced in Korea (한국산(韓國產) 맹종죽(孟宗竹) 죽순(竹筍)의 성분(成分)에 관(關)한 연구(硏究))

  • Cheong, Jong Seong;Park, Nam Chang;Lee, Chung Woo;Whon, Joo Sang
    • Journal of Korean Society of Forest Science
    • /
    • v.78 no.1
    • /
    • pp.55-60
    • /
    • 1989
  • To find the nutritive values of the edible bamboo shoot (Playllostachys edulis) produced in Korea, the component analysis was conducted using the fresh and canned bamboo shoot. As the principal component ; water, protein, lipid, sucrose, ash, cellulose and calorie content were measured in fresh bamboo shoot and canned bamboo shoot. Also the content of minerals ; K, P, Ca, Fe and Vitamins were analysed and compared between fresh and canned bamboo shoot. The contents of component were devided into upper, middle and bottom bamboo parts. The contents of protein, lipid, ash, cellulose and calories of raw bamboo shoot were higher than those of canned shoot. Generally, the components of bamboo shoot produced in Korea were not significantly different to those of Ueda's results in Japan. But the contents of lipid, calcium and potassium were higher in Korean bamboo shoot.

  • PDF

Effect of Tex1/THOC3, a component of THO complex, on growth and mRNA export in fission yeast (분열효모에서 THO 복합체의 구성요소인 Tex1/THOC3가 생장 및 mRNA 방출에 미치는 영향)

  • Bae, Soo Jeong;Koh, Eun-Jin;Yoon, Jin Ho
    • Korean Journal of Microbiology
    • /
    • v.53 no.4
    • /
    • pp.292-296
    • /
    • 2017
  • In eukaryote, THO/TREX complex plays a critical role in transcriptional elongation, pre-mRNA processing, and nuclear mRNA export. This complex is evolutionally well- conserved, but there are some differences in composition and function according to organisms. Here we showed that spTex1, a component of THO/TREX complex, is not essential for growth and mRNA export in a fission yeast, Schizosaccharomyces pombe, which is more similar to higher eukaryote than budding yeast. Deletion and overexpression of the spTex1 gene do not lead to any detectable growth phenotype and accumulation of poly(A)+ RNA in the nucleus. And the spTex1-GFP protein is localized mainly in the nucleus. Yeast two-hybrid and Co-immunoprecipitation analysis showed that the spTex1 protein interacted with spHpr1 (THOC1) and spTho2 (THOC2), main subunits of THO complex. We conclude that the S. pombe Tex1 is a component of THO/TREX complex, but does not plays important roles in growth and bulk mRNA export from the nucleus.

A Clinical Proterties on Nutrients Intake and Serum Concentration of Diabetic Subjects by Duration in Ansung Kyunggi-do (경기도 안성지역 당뇨병 환자의 유병기간에 따른 영양소 섭취 실태와 혈액성상의 임상적 특징)

  • 노숙령
    • Journal of Nutrition and Health
    • /
    • v.30 no.10
    • /
    • pp.1203-1210
    • /
    • 1997
  • This study was performed from March to June 1994 in ANsung Medical Center. We investigated serum components of 78 diabetics in the center, in addition to the nutrient intake status and anthrophometric status of 60 diabetics among them. We separated the latter into 2 groups(ⅰ) less tan 5 years of diabetic duration and ⅱ) more than 6 years of diabetic duration) and analyzed the clinical characteristics of each group. The results are as follows : \circled1 The average ages of male and female subjects were 57.1 and 58.9 years, respectively. The average diabetic duration of male subjects was 4.8 years and that of female subjects was 5.9 . The major occupational field of both male and female subjects was agriculture(33.3%, 36.1%). 50% of male and 75% of female subject's monthly incomes were less than 600, 000 won and 75% of male and 94.4% of female subjects had weekly food expense less than 40, 000 won. The nutrient intake status of the subjects was lower than RDA on the whole. There was no big differences between 2 the male groups of different diabetic duration , but calorie, protein , fat carbohydrate, phosphorus, iron and Vit B$_1$intakes of the above 6 year female group were appearently higher than those of the under 5 year female group (p<0.05). There were different intake tendencies between male and female subjects . For females. the longer the diabetic duration, the less intakes of calorie, protein , fat carbohydrate, phosphorus, iron Vit B$_1$, Vit B$_2$, and niacin were observed. Furthermore, the longer the diabetic duration in females , the more intakes of energy and nutrients, except Vit C were observed. Fasting blood glucose levels were 155mg/dl and 164mg/dl and Postprandial glucose levels were 242mg/dl and 247mg/dl for males and females respectively. Hb of both males and females , and Ht of males met the lower limit of normal status, The serum TG level was higher than normal , so that they seemed to suffer from hyperlipidemia ; their high HbAIC status indicated that the management of diabetes mellitus was poor. The total protein level of female subject was higher when compared with that of males(p<0.05). All subjects blood pressure were higher than normal , and the longer the diabetic duration, the higher was the blood pressure. The serum HDL-cholesterol levels of the above 6 year group male subjects were lower(p<0.05) than those of the under 5 year male group subjects, and the former group's systolic and diastolic blood pressures were higher than those of the latter(p<0.05). Levels of serum total protein, albumin and TG of the above 6 year female group were lower than those of the under 5 year female group(p<0.05). As the diabetic duration was extended, HDL-cholesterol and LDL-cholesterol levels showed a tendency to increase, while Hb, Ht, total protein, albumin, and A/G, which represent the nutrients state of iron and protein, were inclined to decrease. This result showed that serum lipid components went bad. To summarize the results, the longer the diabetic duration, the worse were the male subjects nutrient intake status, but those of the female subjects were good. With respect to serum components and serum lipid subjects Fe, protein, nutrition status and serum lipid component got worse as the diabetic duration was extended , implying the possibility of diabetes mellitus complication grows.

  • PDF

Development of hypothermic preservation solution for the human dermal fibroblast using protein hydrolysates (단백질 가수분해 물을 이용한 인간 피부 섬유아세포의 저온 보존액 개발)

  • Byoun, Soon-Hwi;Choe, Tae-Boo
    • KSBB Journal
    • /
    • v.24 no.3
    • /
    • pp.312-320
    • /
    • 2009
  • Stable cell preservation is an essential factor in the regenerative medicine for cell therapies and transplantation of biologic materials. In this study, we studied to provide more stable hypothermic preservation by protection of cell damage during the preservation at $4^{\circ}C$. The result of searching for key components that have excellent efficacy in hypothermic preservation of cells, we have identified the fact that the hypothermic preservation adding protein hydrolysates such as yeast hydrolysate is far superior to others. All protein hydrolysates that are derived from animal, plant and microbe sources have superior efficacy, especially the peptides which have molecular weights under 10 kDa have the best efficacy among the components of protein hydrolysate. The protein hydrolysates prevented the decrease of ATP level in the cells caused by hypothermic environment and they inhibited the generation of ROS. Adding antioxidants and control agents of osmotic pressure were showed to have more superior efficacy in hypothermic preservation. Finally, KUL261 solution (DMEM/F12 1 : 1 medium, yeastolate 1%, $\alpha$-tocopherol $100{\mu}M$, dextran 2.5%), the preservation solution developed in this study, showed the best efficacy in both cell viability and cell growth more than other conventional preservation solutions. In conclusion, the improved hypothermic preservation solution that contains the protein hydrolysates as a key component provide the best preservation efficacy. It provides better efficacy than other preservation solutions and will contribute to both the development of regenerative medicine and global commercialization in this therapeutic field.

Design and Implementation of an Analysis System for Diseases and Protein Based on Components (컴포넌트 기반의 질병 및 단백질 분석 시스템의 설계 및 구현)

  • Park, Jun-Ho;Yeo, Myung-Ho;Lee, Ji-Hee;He, Li;Kang, Gwang-Goo;Kwon, Hyun-Ho;Lee, Jin-Ju;Lee, Hyo-Joon;Lim, Jong-Tae;Jang, Yong-Jin;WeiWei, Bao;Kim, Mi-Kyoung;Ryu, Jae-Woon;Kang, Tae-Ho;Kim, Hak-Yong;Yoo, Jae-Soo
    • The Journal of the Korea Contents Association
    • /
    • v.10 no.12
    • /
    • pp.59-69
    • /
    • 2010
  • The research on protein for the diseases analysis and the new medicines development is one of the most important themes in biotechnology. Since the analysis on diseases and protein needs to handle a large scale of data, we don't use the way to approach it by the experiments anymore. In recent, we have accelerated the research on diseases and protein analysis by sharing and connecting the various experimental data by combining the biotechnology with the IT technology. However, many biotechnology researchers have difficulty in handling the protein analysis tools based on the IT knowledge. In order to solve such problems, data analysis tools through the cooperation between IT researchers and biologists have been developed. However, the existing data analysis tools still have the problems that it is very hard for biologists to extend their functions and to use them. In this paper, we design and implement an effective analysis system for diseases and protein based on components that alleviates the problems of the existing data analysis systems.

Identification of Brassinosteroid-Related Protein, BAK1 from Nutrition Deficient Tomato Cultivated by Soilless Cultivation System (수경재배 영양결핍토마토에서 브레시노스테로이드관련 신호전달 단백질 BAK1의 동정)

  • Shin, Pyung-Gyun;Chang, An-Cheol;Hong, Sung-Chang;Lee, Ki-Sang
    • Journal of Life Science
    • /
    • v.17 no.12
    • /
    • pp.1729-1733
    • /
    • 2007
  • Brassinolide insensitive associated receptor kinase 1(BAK1) is a critical component that play an important roles in signaling of brassinosteroid biosynthesis. Brassinosteroid-deficient and -insensitive mutants showed the characteristic of dwarf symptom. The nutrient deficient tomato showing stunt phenomenon was selected from soiless cultivation system using modified Sonneveld hydroponic solution. Twenty eight protein spots showing different expression levels compared to the control were isolated from extracts of stunted tomato leaves by 2D PAGE analyses. Significantly down-regulated 6 protein spots out of 28 protein spots were analyzed and sequenced by MALDI-TOF mass spectrometry. The protein spot having pI=4.5 and MW=24 kDa was identified as a signal protein, BAK1, which is directly related to brassinosteroid biosynthesis. In addition, five other protein spots were identified as BCK1, cystein proteinase, sulfutase, peroxidase and zinc finger factor respectively, and they were also signal proteins related to brassinosteroid biosynthesis. Furthermore, amplification of 500bp of BAK1 mRNA by RT-PCR using a primer set of peptide matched regions was inhibited conpared to that of the wild type. The results sugested that the BAK1 might be regulated at the transcription level in response to nutrition applications.

Effect of Seed Coat Color and Seed Weight on Protein, Oil and Fatty Acid Contents in Seeds of Soybean (Glycine max (L.) Merr.) Germplasms

  • Yu-Mi Choi;Hyemyeong Yoon;Myoung-Jae Shin;Yoonjung Lee;On Sook Hur;XiaoHan Wang;Kebede Taye Desta
    • Proceedings of the Plant Resources Society of Korea Conference
    • /
    • 2021.04a
    • /
    • pp.15-15
    • /
    • 2021
  • Seed coat color and seed weight are among the key agronomical traits that determine the nutritional quality of soybean seeds. This study aimed to evaluate the contents of total protein, total oil and five prominent fatty acids in seeds of 49 soybean varieties recently cultivated in Korea, and assess the influences of seed coat color and seed weight on each. Total protein and total oil contents were in the ranges of 36.28-44.19% and 13.45-19.20%, respectively. Likewise, individual fatty acid contents were in the ranges of 9.90-12.55, 2.45-4.00, 14.97-38.74, 43.22-60.26, and 5.37-12.33% for palmitic, stearic, oleic, linoleic, and linolenic acids, respectively. Our results found significant variations of protein, oil and fatty acid contents between the soybean varieties. Moreover, both seed coat color and seed weight significantly affected total oil and fatty acid contents. Total protein content, however, was not significantly affected by any factor. Among colored soybeans, pale-yellow soybeans were characterized by a high level of oleic acid (30.70%) and low levels of stearic (2.72%), linoleic (49.30%) and linolenic (6.44%) acids, each being significantly different from the rest of colored soybeans (p < 0.05). On the other hand, small soybeans were characterized by high levels of all individual fatty acids except oleic acid. The level of oleic acid was significantly high in large seeds. Cluster analysis grouped the soybeans into two classes with notable content differences. Principal component analysis also revealed fatty acids as the prime factors for the variability observed among the soybean varieties. As expected, total oil and total protein contents showed a negative association with each other (r = -0.714, p < 0.0001). Besides, oleic acid and linoleic acid showed a tradeoff relationship (r = -0.936, p < 0.0001) which was reflected with respect to both seed coat color and seed weight. In general, the results of this study shade light on the significance of seed coat color and seed weight to distinguish soybeans in terms of protein, oil and fatty acid contents. Moreover, the soybean varieties with distinct characteristics and nutritional contents identified in this study could be important genetic resources for consumption and cultivar development.

  • PDF

Investigation of Heavy Metals, Residual Pesticides and Nutrient Component from Agricultural By-products Imported as Medium Substrates for Mushroom Cultivation (버섯 재배용 배지 재료로 수입한 농업부산물에서 중금속, 잔류농약, 영양성분 조사)

  • Kim, Jun Young;Lee, Geun Sick;Lee, Chan Jung;Kim, Seong Hwan
    • Korean Journal of Environmental Agriculture
    • /
    • v.36 no.3
    • /
    • pp.217-221
    • /
    • 2017
  • BACKGROUND: For the food safety of cultivated mushroom, information on the safety of agricultural by-products imported as medium substrates for mushroom cultivation is urgently needed. Therefore, this study was performed to detect the presence of heavy metals, residual pesticides, and nutrient component in the imported medium substrates. METHODS AND RESULTS: Six kinds of medium substrates imported from nine countries from 2015 to 2017 were investigated. A mercury analyzer MA-2000 and an inductively coupled plasma spectrometer OPTIMA 7000DV were used to analyze mercury, lead, arsenic, copper, nickel and cadmium. All of these heavy metals were detected at lower level than heavy metal tolerance standard level of by-product fertilizer in Korea. When 246 kinds of residual pesticides were examined by GC and HPLC, imidacloprid, thiamethoxam and carbendazim were detected from Egyptian beet pulp, Indian cottonseed meal and cottonseed hull, respectively. The content of nutrient components (water, crude ash, crude fat, crude protein and crude fiber) varied among imported countries and the medium substrates. CONCLUSION:The presence of heavy metals and residual pesticides in imported medium substrates for mushroom cultivation was confirmed. For the safe production of mushroom, this study shows that imported medium materials for mushroom cultivation need to be managed through continuous monitoring.

Physicochemical and sensory characteristics of pizza dough prepared with domestic and imported wheat flour (우리밀과 수입밀로 제조한 피자반죽의 이화학적 및 관능적 특성 비교)

  • Choi, Jae Suk;Lee, Su Han;Rha, Young-Ah;Kim, Jung Hoan
    • Korean Journal of Food Science and Technology
    • /
    • v.48 no.4
    • /
    • pp.366-371
    • /
    • 2016
  • This study was carried out to evaluate the feasibility of using domestic wheat flour as ingredient of pizza dough by examining the physicochemical and sensory properties of the dough. Five pizza dough samples were prepared with different ratio of domestic and imported wheat flour. Significant differences were observed in crude protein content, crude ash content, and color of the pizza dough samples; however, no significant difference was found in mechanically measured rheology. A sensory evaluation based on 23 descriptive attributes indicated significant differences between pizza dough made from domestic wheat flour and that made from imported wheat flour for springiness and softness of the surface, roughness, and chewiness (p<0.05). Principal component analysis showed that the greater amount of Korean wheat flour in the dough resulted in different values from negative to positive ends of principal component 1 (37.4%).

Comparisons of Food Component Characteristics of Wild and Cultured Edible Pufferfishes in Korea (자연산과 양식산 식용복어의 성분 비교)

  • Hwang, Seok-Min;Oh, Kwang-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.46 no.6
    • /
    • pp.725-732
    • /
    • 2013
  • To identify the food component characteristics of seven edible pufferfishes (five wild pufferfishes including striped puffer Takifugu xanthopterus; eyespot puffer Takifugu chinensis; purple puffer Takifugu porphyreus; rough-backed puffer Lagocephalus wheeleri; and grass puffer Takifugu niphobles; and two cultured pufferfishes including yellow puffer Takifugu obscurus, and tiger puffer Takifugu rubripes) in Korea, the proximate, fatty/amino acid, chemical and taste compositions were investigated. The proximate compositions were not significantly different among the sampled pufferfishes, whereas grass puffer had lower moisture and crude lipid levels contents, and higher crude protein and ash contents than the other species. The total amino acid levels in wild and cultured pufferfishes were 14,941.6-16,427.9 mg/100 g, and the major amino acids were aspartic acid, glutamic acid, glycine, alanine, leucine, lysine and arginine. Regarding the fatty acid and mineral compositions, the major fatty acids included 22:6n-3, 16:0, 18:1n-9, 20:5n-3, 18:0, 20:4n-3, and 22:5n-3 in that order, and there was a little difference among the sampled pufferfishes. Grass puffer had a higher monoenes ratio, i.e., 16:1n-7 and 18:1n-9, and a lower polyenes ratio than the other pufferfishes. The main minerals detected K, P, Na, S and Ca. Regarding the taste-active compounds, the free amino acid contents of pufferfish extracts were 236.4-428.1 mg/100 g, consisting primarily of taurine, lysine, proline, glycine, alanine and arginine in that order. The amino nitrogen content of the pufferfishes was 84.5-156.4 mg/100 g, and there was a little difference among the species. As for taste intensity, the total taste value of the purple puffer was higher than that of the other pufferfishes.