• 생명과학회지
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• 제14권3호
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• pp.453-458
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• 2004

콩의 oil 및 단백질은 식품에서 매우 중용한 영양학적인 구성요소이다. Oil및 단백질과 같은 종자 구성물질들은 polygenetic 형질들로 되어있다. 본 시험은 큰올콩과${\times}$익산10호의 RIL 계통과 SSR marker를 이용하여 유전자 지도를 작성하고, 이를 바탕으로 oil 및 단백질 함량과 관련된 양적형질 유전자좌(QTLs)를 탐색하였다. Oil함량과 관련된 QTLs는 연관군 C2와 satt100과 연관군 DIb+W의 satt546및 연관군 L의 satt418의 세 개의 독립적인 QTLs를 확인하였다. 단백질 함량에 있어서는 연관군 B2와 J 및 L에 각각 satt556과 satt414 및 satt238의 marker에서 독립적인 QTLs를 확인하였다. 본시험의 결과, oil 및 단백질 함량과 관련된 공통의 QTL은 연관군 L이었다. 한편, oil 및 단백질과 같은 종자구성물질은 주로 환경적인 stress및 종자의 크기 등에 의해서 구성되어지는 것으로 생각된다.

• 한국수산과학회지
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• 제53권2호
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• pp.165-173
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• 2020

Fish roe is among the most valuable food resources produced by fisheries. Raw fish roe requires processing for conversion into hygienic, marketable, and consumer-acceptable forms. In this study, to investigate the food compositional characteristics of various types of fish roe, we applied vacuum freeze-drying to prepare fish roe concentrates (FRCs) from roe of Alaska pollack Theragra chlcogramma, bastard halibut Paralichythys olivaceus, and skipjack tuna Katsuwonus pelamis. The FRC yield ranged from 22.7 to 26.7 g/100 g roe. The major constituents of FRCs were protein (65.4-79.6%), moisture (2.8-6.2%), lipids (8.5-18.3%), and ash (4.8-7.2%). Potassium, sulfur, sodium, and phosphorus were the major mineral elements of FRCs, and the major amino acids were aspartic acid (9.0-10.4 g/100 g protein), glutamic acid (13.2-14.5 g/100 g protein), lysine (8.4-8.6 g/100 g protein), and leucine (8.3-9.7 g/100 g protein). Vacuum freeze-dried FRCs differed among fish species in terms of amino acid composition and electrophoresis protein band distribution. Therefore, FRCs are an excellent source of protein nutrition and an appropriate protein fortification material in human foods or animal feed.

• The Plant Pathology Journal
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• 제23권4호
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• pp.281-286
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• 2007

Interactions between viral proteins and host proteins are essential for virus replication. Especially, translation of viral genes completely depends on the host machinery. In potyviruses, interactions of genome-linked viral protein (VPg) with host translation factors including eIF4E, eIF(iso)4E, and poly(A)-binding protein (PABP) has previously been characterized. In this study, we investigated interactions between Soybean mosaic virus (SMV) viral proteins and host translation factors by yeast two-hybrid system. SMV VPg interacted with eIF4E, eIF(iso)4E, and PABP in yeast two-hybrid system, while SMV helper component proteinase (HC-pro) interacted with neither of those proteins. The interaction between SMV NIb and PABP was also detected. These results are consistent with those reported previously in other potyviruses. Interestingly, we found reproducible and specific interactions between SMV coat protein (CP) and PABP. Deletion analysis showed that the region of CP comprising amino acids 116 to 206 and the region of PABP comprising amino acids 520 to 580 are involved in CP/PABP interactions. Soybean library screening with SMV NIb by yeast two-hybrid assay also identified several soybean proteins including chlorophyll a/b binding preprotein, photo-system I-N subunit, ribulose 1,5-biphosphate carboxylase, ST-LSI protein, translation initiation factor 1, TIR-NBS type R protein, RNA binding protein, ubiquitin, and LRR protein kinase. Altogether, these results suggest that potyviral replicase may comprise a multi-protein complex with PABP, CP, and other host factors.

• Journal of Gastric Cancer
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• 제3권2호
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• pp.75-79
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• 2003

Purpose: Evidence exists that dysregulation of apoptosis is involved in the pathogenesis of cancer development. The Bcl-$x_{L}$/Bcl-2-associated death promoter (BAD), a member of the Bcl-2 family, is a critical regulatory component of the intrinsic cell-death pathway that exerts its pro-apoptotic effect upon heterodimerization with anti-apoptotic proteins Bcl-2 and Bcl-$X_{L}$. Expression of the BAD protein has been reported in several cancer types, but not in stomach cancer. The aim of this study was to explore the expression status of the BAD protein in gastric carcinomas. Materials and Methods: In the current study, we analyzed the expression of the BAD protein in 60 advanced gastric adenocarcinomas by using immunohistochemistry and a tissue microarray approach. Results: Immunopositivity (defined as $\geq\30\%$) was observed for the BAD protein in 57 ($95\%$) of the 60 cancers. Normal gastric mucosal cells showed weaker expressions of the BAD protein than gastric carcinomas. Conclusion: Taken together, these results suggest that stomach cancer cells in vivo may need BAD protein expression for apoptosis. Also, the higher expression of the BAD protein in stomach cancer cells than in normal gastric mucosal cells suggests that apoptosis might be easily triggered in susceptible stomach cancer cells, thereby producing selective pressure to make more apoptosis-resistant cells during tumor development.

• 한국환경과학회지
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• 제31권3호
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• pp.285-289
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• 2022

This study investigated the effects of dietary Ptecticu tenebrifer powder and canned mixtures on protein digestibility by different breeds of companion dogs (15 Bichons, 15 Malteses, 15 Chihuahuas and 15 Poodles). The mixtures were divided into Diet A, Diet B, Diet C, Diet D, and Diet E, which were supplied from five farms. Twenty-five grams each was mixed with 100 g of each canned food, and a total of 125 g was measured for each breed of dog. The result of component analysis of the mixtures showed the highest protein contents rather than dry matter, crude ash or crude fat. There were statistical significances (p<0.05) in all mixed feeds fed to bichon, maltese, chihuahua and poodle dog. Overall, protein digestibility by the breeds of dog ranged from 87.44% to 97.18%. Result of breed of dog comparison revealed that Diet E by poodle dog had the highest protein digestibility, and the lowest protein digestibility was observed in Diet C by Maltese. In conclusion, the use of dietary Ptecticu tenebrifer powder and canned mixtures did not only increased protein digestibility by different breeds of dog but also maintained normal manure properties.

• Parasites, Hosts and Diseases
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• 제28권2호
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• pp.101-108
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• 1990

포도낭미충증을 포함한 유구낭미충증 환자의 신경외과적 수술 과정에서 수거한 낭미충 낭액의 성분 단백질을 비교 검토하였다. 7.5% gel에서 Disc-PAGE와 reducing condition의 10~15% gel에 SDS-PAGE를 실시하여 다음과 같은 결과를 얻었다. 1. 7.5% gel에서 실시한 Disc-PAGE 소견상 제주도와 Ecuador에서 수집한 돼지의 유구낭미충 낭액은 U, A, B, C, E, S의 6개 band로 구성되어 있었고 그 중 band C가 주 구성 성분이었다. 그리고 인체에서 수집한 낭액 은 band C가 주 구성 성분이었고 경우에 따라 band E가 주 구성 성분으로 관찰되고 있었다. 정상 뇌척수액은 band C, E 위치에서 ${\gamma}-globulin$과 albumin이 보이고 있었다. 2. 10~15% gel에서 실시한 SDS-PAGE상 제주도 낭액은 최소 25개 이상의 subunit로 구성되어 있었고 그 중 95, 64, 48, 39, 34, 24, 15, 10및 7 kDa band가 주 구성 성분이었다. 그리고 Ecuador감염 돼지의 유구낭미충 낭액은 전체적으로 비슷했으나 48, 39, 34, 24 kDa band가 의미한 반면 21, 17 kDa가 진하게 염색되었다. 인체 뇌낭미충증 환자에서 수집한 낭액들은 15~21개 정도의 band가 보이고 있었다. 그 중 94, 64, 15, 10 그리고 7 kDa band는 모든 낭액에 공통적으로 포함되어 있었다. 한편, 정상 뇌척수액의 SDS-PAGE에서는 77, 66 (albumin), 55 (heavy chain of ${\gamma}-globulin$), 22.5 (light chain of ${\gamma}-globulin$) 그리고 17 kDa band가 보였다. 환자의 낭액은 77, 66, 55, 22.5 kDa에서 진하게 염색되는 경우가 있었다. 이상의 결과로 인채 유구낭미충증에서 수집한 낭액에도 15, 10, 7kDa의 subunit로 구성된 150kDa의 band C protein이 주 성분으로 구성되어 있으며 수술장에서 수집하는 과정에서 뇌척수액이 섞이는 경우가 많음을 알 수 있었다.

• 동아시아식생활학회지
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• 제16권1호
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• pp.23-28
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• 2006

The purpose of this paper was to clarify the actual conditions of the 'Dietary externalization' mainly by using the economic and nutrition-related data, accompanied by the economic development in Korea and Japan. 'Modernization of food style' and other modernization have taken place, among which 'Dietary externalization' in particular has recently drawn interest. At the time this paper clarified with econometric analysis whether there are differences between the two countries in term of the modernization of food style and dietary externalization trend. The trends of Dietary externalization of both Korea and Japan were studied using Principal Component Analysis method. The food subgroup were investigated based on the annual report on the household income and expenditure survey of Korea and the annual report on the family income and expenditure survey of Japan. The statistical data from both country were analyzed by SAS program. The results are as follows; 1. In Korea, the ratio of carbohydrates in the total calorie intake is quite high and animal protein is rather low compared to those in Japan. 2. Traditional food such as grains and vegetables are consumed much more in Korea than in Japan. 3. The Principal Component 1, 2 were extracted in both countries during the whole analysis period, which suggested the 'Dietary externalization' 4. Principal Component 1 has a positive factor loaded in all food items including meals outside the home and process food. In other words, it is apparent that the 'Dietary externalization' tread in Korea has a simple pattern suggesting that all externalization related items are on the rise. 5. Principal component 1, 2 which indicated the dietary externalization, were detected in Japan.

• Journal of Nutrition and Health
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• 제6권2호
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• pp.1-16
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• 1973

Some effects of dietary conditions on the metabolism of nucleic acid and protein in organs of the Albino Rats have been studied. The young rats to be examined were fed on the control diet and the diets deprived of one component among protein, carbohydrate, and lipid, such as protein free diet (PF: 432 kcal/100g) carbohydrate free diet (CF: 432kcal/100g), and lipid free diet (LF: 392kcal/100g) for three, seven, and fifteen days, respectively. The contents of DNA and RNA in the liver and the brain, and also those of protein-nitrogen(PN) and nonprotein-nitrogen (NPN) in the live, the brain, and the serum have been measured. The results are as followe: 1. The contents of DNA per gram of liver were increased by feeding on protein free diet. It is concluded that the critical factor for the result is not the increase in the rates of DNA syntheses, but the decrease in the turnover rates of DNA. 2. The metabolism of DNA in the liver showed the normal status by feeding on carbohydrate free diet. On the other hand, the rates of DNA syntheses were increased by feeding on lipid free diet. 3. The rates of DNA syntheses in the brain were decreased by feeding on the unbalanced diet, such as protein free, carbohydrete free, and lipid free diet. 4. In the liver and the brain, the rates of DNA syntheses were decreased by feeding on protein free diet. But the rates showed the normal status by feeding on the carbohydrate free diet, and also showed the similar metabolism to that in the case of the control group by feeding on lipid free diet. 5. In the liver, the rates of protein syntheses were decreased, whereas the contents of nonprotein-nitrogen were increased by feeding on protein free diet. 6. In the liver and the brain, the protein syntheses showed the more increasing rates than the rates in the case of the control diet by feeding on lipid free diet. 7. In the serum, the contents of protein did not change in a short period, also the insufficient feeling on protein was examined. It is clear that in the liver the rates of protein syntheses are decreased and the rates of protein catabolism are increased, since the rates of nucleic acid syntheses are decreased by feeding on the protein free diet. On the other hand, it is considered that in the brain the turnover rates of protein does not have correlation with the rates of nucleic acid syntheses, also these are decreased by feeding on protein free diet. And also it is believed that the phenomena of homeostasis for carrying the normal metabolism of nucleic acid and protein in the liver and the brain are operated in a short period as possible, by feeding on carbohydrate free and lipid free diets.

• BMB Reports
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• 제42권9호
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• pp.541-551
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• 2009

Amyloidogenesis defines a condition in which a soluble and innocuous protein turns to insoluble protein aggregates known as amyloid fibrils. This protein suprastructure derived via chemically specific molecular self-assembly process has been commonly observed in various neurodegenerative disorders such as Alzheimer's, Parkinson's, and Prion diseases. Although the major culprit for the cellular degeneration in the diseases remains unsettled, amyloidogenesis is considered to be etiologically involved. Recent recognition of fibrillar polymorphism observed mostly from in vitro amyloidogeneses may indicate that multiple mechanisms for the amyloid fibril formation would be operated. Nucleation-dependent fibrillation is the prevalent model for assessing the self-assembly process. Following thermodynamically unfavorable seed formation, monomeric polypeptides bind to the seeds by exerting structural adjustments to the template, which leads to accelerated amyloid fibril formation. In this review, we propose another in vitro model of amyloidogenesis named double-concerted fibrillation. Here, two consecutive assembly processes of monomers and subsequent oligomeric species are responsible for the amyloid fibril formation of $\alpha$-synuclein, a pathological component of Parkinson's disease, following structural rearrangement within the oligomers which then act as a growing unit for the fibrillation.

• Bulletin of the Korean Chemical Society
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• 제33권9호
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• pp.3071-3075
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• 2012

Human ${\alpha}$-synuclein is the major component of the protein aggregates known as Lewy bodies or Lewy neurites, which define the intracellular lesions of Parkinson's disease. Despite extensive efforts, the physiological function of ${\alpha}$-synuclein has not yet been elucidated in detail. As an approach to defining its function, proteins that interacted with ${\alpha}$-synuclein were screened in phage display assays. The SNARE protein vesicle t-SNARE-interacting protein homologous 1B (VTI1B) was identified as an interacting partner. A selective interaction between ${\alpha}$-synuclein and VTI1B was confirmed by coimmunoprecipitation and GST pull-down assays. VTI1B and ${\alpha}$-synuclein were colocalized in N2a neuronal cells, and overexpression of ${\alpha}$-synuclein changed the subcellular localization of VTI1B to be more dispersed throughout the cytosol. Considering the role played by VTI1B, ${\alpha}$-synuclein is likely to modulate vesicle trafficking by interacting with a SNARE complex.