• Transactions of the Korean Society of Machine Tool Engineers
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• v.11 no.6
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• pp.75-82
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• 2002

Design for a quiet operation of fluid power system requires the understanding of noise and vibration characteristics of the system. It's not easy to analyze noise problem in hydraulic cylinder used in typical actuator Because they've got complex fluid dynamics. One of the fundamental problems associated with the hydraulic system is the pulsating flow in pipe lines, which can be tackled by the analysis under simplifying assumptions. The present study focuses on theoretic analysis and experimental study on the dynamics of laminar pulsating flow in a circular pipe. We analyze the propagation characteristics of the pressure pulse within a hydraulic pipe line taking into account the pulsating flow frequency variation. We also measure instantaneous pressure pulses within pipe line to identify the transfer functions. We conduct series of experiments to investigate the propagation characteristics of pressure pulse for various pressure of pulsating flow. The working fluid of the present study is ISO VG46 and the temperature ranges from 20 to $60^{\circ}$ with normal pressure at 4000kPa. The flow rate is measured by using an ultrasonic flow meter. Pressures at fixed upstream and downstream positions are measured concurrently. The electric signals of the pressure sensor are stored and analyzed using a system analyzer(PKE 983 series). The frequency is varied in the range of 10~500Hz. The Reynolds number is kept below 2,000. In the present study, boundary condition was varied by installing a surge tank and an orifice at the end of pipe. Experimental and theoretical results were compared each other under various boundary conditions.

• YAKHAK HOEJI
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• v.39 no.2
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• pp.161-168
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• 1995

Brain dopamine systems play a central role in the control of movement, hormone release, and many complex behavior. The action of dopamine at its synapse is terminated predominately by high affinity reuptake into presynaptic terminals by dopamine transporter (DAT). The dopamine transporter(DAT) is membrane protein localized to dopamine-containing nerve terminals and closely related with cocaine abuse, Parkinsonism, and schizophrenia. In present study, the recombinant plasmid pRc/CMV-DAT, constructed by subcloning of a cDNA encoding a bovine DAT into eukaryotic expression vector pRc/CMV, was stably transfected into CV-1 cells(monkey kidney cell line). The DAT activities in the cell lines selected by Geneticin$^{R}$ were determined by measuring the uptake of $[^3H]$-dopamine. The transfected cell lines showed 30-50 fold higher activities than untransfected CV-1 cell line, and this result implies that DAT is well expressed and localized in transfected cells. The transfected cells accumulated $[^3H]$-dopamine in a dose-dependent manner with a $K_{m}$ of 991.6nM. Even though high doses of norepinephrine, epinephrine, serotonin, and choline neurotransmitters inhibited the uptake of $[^3H]$-dopamine, DAT in transfected cell line was proven to be much more specific to dopamine. The psychotropic drugs such as GBR12909, CFT, normifensine, clomipramine, desipramine, and imipramine inhibited significantly the dopamine uptake in tissue culture cells stably transfected with DAT cDNA. Radioactive in situ hybridization was done to map the cellular localization of DAT mRNA-containing cells in the adult rat central nervous system. The strong hybridization signals were detected only in the substantia nigra pars compacta and ventral tegmental area. The restricted anatomical localization of DAT mRNA-containing cells confirms the DAT as a presynaptic marker of dopamine-containing cells in the rat brain.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.45 no.8
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• pp.60-68
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• 2008

The complex permittivity and permeability of various periodic metamaterial (MTM) cells are extracted by simulating a fictitious rectangular waveguide consisting of PEC and PMC walls. The shapes of the MTM cells include a thin wire (TW), a single split-ring resonator (SSRR), a double SRR (DSRR), a modified SRR, and a combined structure of the TW and the DSRR. The TW falls on a negative-$\varepsilon$/positive-$\mu$ region, the SRRs on a positive-$\varepsilon$/negative-$\mu$ region, and the combined structure on a negative-$\varepsilon$/negative-$\mu$ region. We also investigate how the permeability and permeability are affected by the dimension parameters of the MTM cells. Another extraction technique utilizing time domain signals is developed overcoming some limitations that the waveguide technique can not handle.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2017.05a
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• pp.285-289
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• 2017

Laser-induced breakdown spesctroscopy (LIBS) is a technique that complements the disadvantages of conventional laser-based combustion diagnosis techniques such as weak signal strength, complex equipment configuration, and low accuracy. In this study, basic research was carried out to measure the combustion gas temperature of scramjet engines using LIBS. Spray flames were generated from Jet A-1 fuel used in scramjet engines and gas temperatures were measured at the top of the flames with a calibrated thermocouple. The LIBS signals were acquired at the same points as the temperature measurement positions of the thermocouple. The LIBS spectra were analyzed to obtained a calibration curve between the LIBS signal and the reference temperature measured at the thermocouple. Therefore, it was confirmed that the combustion gas temperature can be measured in-situ using LIBS.

• Molecules and Cells
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• v.38 no.2
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• pp.105-111
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• 2015

The beta2-adrenergic receptor (${\beta}2AR$) belongs to the G protein coupled receptor (GPCR) family, which is the largest family of cell surface receptors in humans. Extra attention has been focused on the human GPCRs because they have been studied as important protein targets for pharmaceutical drug development. In fact, approximately 40% of marketed drugs directly work on GPCRs. GPCRs respond to various extracellular stimuli, such as sensory signals, neurotransmitters, chemokines, and hormones, to induce structural changes at the cytoplasmic surface, activating downstream signaling pathways, primarily through interactions with heterotrimeric G proteins or through G-protein independent pathways, such as arrestin. Most GPCRs, except for rhodhopsin, which contains covalently linked 11 cis-retinal, bind to diffusible ligands, having various conformational states between inactive and active structures. The first human GPCR structure was determined using an inverse agonist bound ${\beta}2AR$ in 2007 and since then, more than 20 distinct GPCR structures have been solved. However, most GPCR structures were solved as inactive forms, and an agonist bound fully active structure is still hard to obtain. In a structural point of view, ${\beta}2AR$ is relatively well studied since its fully active structure as a complex with G protein as well as several inactive structures are available. The structural comparison of inactive and active states gives an important clue in understanding the activation mechanism of ${\beta}2AR$. In this review, structural features of inactive and active states of ${\beta}2AR$, the interaction of ${\beta}2AR$ with heterotrimeric G protein, and the comparison with ${\beta}1AR$ will be discussed.

• Molecules and Cells
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• v.39 no.4
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• pp.345-351
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• 2016

Wound healing is a complex physiological process necessitating the coordinated action of various cell types, signals and microRNAs (miRNAs). However, little is known regarding the role of miRNAs in mediating this process. In the present study, we show that let-7c miRNA is decreased in heat-denatured fibroblasts and that inhibiting let-7c expression leads to the increased proliferation and migration of dermal fibroblasts, whereas the overexpression of let-7c exerts an opposite effect. Further investigation has identified heat shock protein 70 as a direct target of let-7c and has demonstrated that the expression of HSP70 in fibroblasts is negatively correlated with let-7c levels. Moreover, down-regulation of let-7c expression is accompanied by up-regulation of Bcl-2 expression and down-regulation of Bax expression, both of which are the downstream genes of HSP70. Notably, the knockdown of HSP70 by HSP70 siRNA apparently abrogates the stimulatory effect of let-7c inhibitor on heat-denatured fibroblasts proliferation and migration. Overall, we have identified let-7c as a key regulator that inhibits fibroblasts proliferation and migration during wound healing.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.1
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• pp.127-132
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• 2006

The Global Positioning System (GPS), Inertial Navigation System (INS) and Pseudolite (PL) technologies all play very important roles in navigation systems. As an independent navigation system, GPS can provide high precision positioning results which are independent of time. However, the performance will become unreliable when the system experiences high dynamics, or when the receiver is exposed to jamming or RF interference. In comparison to GPS, though INS is autonomous and provides good short-term accuracy, its use as a standalone navigation system is limited due to the time-dependent growth of the inertial sensor errors. PLs are ground-based transmitters that can transmit GPS-like signals. They have some advantages in that their positions can be determined precisely, and the Signal-to-Noise Ratios (SNR) are relatively high. Because their combined performance, in principle, overcomes the shortcomings of the individual systems, the integration of GPS, INS and PL is increasingly receiving attention from researchers. Depending on the desired performance vs complexity, system integration can be carried out at different levels, namely loose, tight and ultra-tight coupling. Compared with loose and tight integration, although it is more complex in terms of system design, ultra-tight integration will be the basis of the next generation of reliable and robust navigation systems. Its main advantages include improved performance under exposure to high dynamics, and jamming and RF interference mitigation. This paper presents an overview of the ultra-tight integration developments and discusses some of the challenging issues.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.1
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• pp.277-282
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• 2006

It is well known that Kalman filtering is an optimal real-time data fusion method for GPS/INS integration. However, it has some limitations in terms of stability, adaptability and observability. A Kalman filter can perform optimally only when its dynamic model is correctly defined and the noise statistics for the measurement and process are completely known. It is found that estimated Kalman filter states could be influenced by several factors, including vehicle dynamic variations, filter tuning results, and environment changes, etc., which are difficult to model. Neural networks can map input-output relationships without apriori knowledge about them; hence a proper designed neural network is capable of learning and extracting these complex relationships with enough training. This paper presents a GPS/INS integrated system that combines Kalman filtering and neural network algorithms to improve navigation solutions during GPS outages. An Extended Kalman filter estimates INS measurement errors, plus position, velocity and attitude errors etc. Kalman filter states, and gives precise navigation solutions while GPS signals are available. At the same time, a multi-layer neural network is trained to map the vehicle dynamics with corresponding Kalman filter states, at the same rate of measurement update. After the output of the neural network meets a similarity threshold, it can be used to correct INS measurements when no GPS measurements are available. Selecting suitable inputs and outputs of the neural network is critical for this hybrid method. Detailed analysis unveils that some Kalman filter states are highly correlated with vehicle dynamic variations. The filter states that heavily impact system navigation solutions are selected as the neural network outputs. The principle of this hybrid method and the neural network design are presented. Field test data are processed to evaluate the performance of the proposed method.

• Journal of Information Technology Services
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• v.10 no.1
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• pp.105-116
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• 2011

With recent spotlight on the, uniquitous computing technology, the need for object of indentification and location infrastructure has increased. Such GPS technolgy must utilize IEEE 802.15.4 Zigbee used for existing wireless sensor network infra as a basice element for user's context-awareness in a uniquitous environement, for effectiveness.Such real-time GPS service is provided in the internal environment where the user would actually are and most high-rise buildlings apply. Underthe assumption, the real-time GPS technology is seperated by each floor, and signals do not get transmitted to other floors, the application on one floor within the high-rise buildling was conducted. This study intends to suggest a floor detection algorithm using IEE 802.15.3/Zigbee's RSSI which supports the accuracy within a couple of meters for the user's the movement between the floors in high-rise buildings in a complex environment. It proposes an floor detection algorithm using IEEE 802.15.4/Zigbee's RSSI which provides accuracy within a radius of few meters for the users movement between the floors for real-time location tracking within high-rise building in a cmoplex environment. Furthermore, for more accurate real-time location tracking, it suggests an algorithm for real-time location tracking using IEEE 802.15.4a/Zigbee's CSS technology based on triangulation. Based on the suggested algorithm, it designs a hybrid real-time location tracking service system in a high-rise buildling and test its functions.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.39A no.5
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• pp.251-257
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• 2014

Digital RDA system is retransmit into the opposite direction of the incident signals. Digital RDA system have a disadventage that this system do not signal classification in multipath environment. because multipath signal is shown as vector sum of multipath signal, digital RDA system required complex signal process for multipath signal classification. In this paper, to solve these problem we propose hybrid digital RDA system which combination of the MUSIC algorithm and the digital RDA system. Proposed system has two modes. First mode is digital RDA mode. Secornd mode is digital beamforming mode. Digital RDA mode is used in situations where the less the impact of multipath. Digital beamforming mode is applied to multipath effects is greater. In secornd mode, we find optimal path using MUSIC algorithm. After than the proposed system uses only the optimal path. Through the proposed system in a multipath environment with digital RDA can be used to supplement a disadvantage.