• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.963-977
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• 2015

The well-characterized gram-positive bacterium Bacillus subtilis is an outstanding industrial candidate for protein expression owing to its single membrane and high capacity of secretion, simplifying the downstream processing of secretory proteins. During the last few years, there has been continuous progress in the illustration of secretion mechanisms and application of this robust host in various fields of life science, such as enzyme production, feed additives, and food and pharmaceutical industries. Here, we review the developments of Bacillus subtilis as a highly promising expression system illuminating strong chemical- and temperatureinducible and other types of promoters, strategies for ribosome-binding-site utilization, and the novel approach of signal peptide selection. Furthermore, we outline the main steps of the Sec pathway and the relevant elements as well as their interactions. In addition, we introduce the latest discoveries of Tat-related complex structures and functions and the countless applications of this full-folded protein secretion pathway. This review also lists some of the current understandings of ATP-binding cassette transporters. According to the extensive knowledge on the genetic modification strategies and molecular biology of Bacillus subtilis, we propose some suggestions and strategies for improving the yield of intended productions. We expect this to promote striking future developments in the optimization and application of this bacterium.

• Journal of Advanced Navigation Technology
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• v.12 no.6
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• pp.634-639
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• 2008

In mobile communication networks, the main power consumption is due to the actual transmissions power. Therefore, power efficiency network structures have gained considerable importance in mobile multi-hop systems and networks in recent years. In this paper, the performance of mobile multi-hop wireless system with M-QAM signal and forward error control (FEC) technique are analyzed The FEC technique uses extra processing power related to encoding and decoding, it is need complex functions to be built into the communication node. The probability of receiving a correct bit and codeword for relaying a data frame over h hop relay station to the final station is evaluated as a function of channel parameter and number of hops, and the distance between the different station.

• Journal of Advanced Navigation Technology
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• v.11 no.2
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• pp.209-216
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• 2007

In this paper, we embodied posture-stabilization and dynamic gait in a walking robot. 10 RC servo motors are used to operate joints. And the joints have enough moving ranges suitable in any walking pattern. Each joint trajectory is generated by cubic spline interpolation method and the stability of the trajectory is verified by using Zero Moment Point from the robot modeling. To avoid complex structure and expression, Zero Moment Point of the biped robot used angular acceleration is suggested. To measure the stability of the biped robot, Tilt sensor and gyro sensor are used. Finally, Personal Computer is used computer monitoring and data processing. Most of computation, such as 10 RC servo motor control, joint trajectory generating, ZMP compensation, sense measuring, etc, was used Digital Signal Processor.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.23 no.5
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• pp.438-444
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• 2013

This paper presents the replication of a desired vibration response by iterative learning control (ILC) system for a vibration motion replication actuator. The vibration motion replication actuator has parameter uncertainties including system nonlinearity and joint nonlinearity. Vehicle manufacturers worldwide are increasingly relying on road simulation facilities that put simulated loads and stresses on vehicles and subassemblies in order to reduce development time. Road simulation algorithm is the key point of developing road simulation system. With the rapid progress of digital signal processing technology, more complex control algorithms including iterative learning control can be utilized. In this paper, ILC algorithm was utilized to produce simultaneously the six channels of desired responses using the Stewart platform composed of six linear electro-magnetic actuators with Halbach magnet array. The convergence rate and accuracy showed reasonable results to meet the requirement. It shows that the algorithm is acceptable to replicate multi-channel vibration responses.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.6C
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• pp.629-636
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• 2006

In array signal processing, high order statistics can be used to estimate parameters from signal of sums of complex exponential. In this paper, we derive two types of direction finding algorithms which use the fourth-order cumulant and moment of the received array data. Since the fourth order cumulant can suppress the Gaussian noise, the response of MPM has better noise immunity than the conventional approaches. The performance of each method in regard to the probability of resolution and SNR in the presence of the Gaussian noise is investigated. As a result, the proposed method applied to the fourth-order statistic can find DOA more correctly in the presence of the Gaussian noise.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2018.10a
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• pp.255-257
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• 2018

Recently, as the frequency of use of video media increases in various fields, the importance of signal processing is increasing. However, many kinds of noise are generated in the transmission and reception process and affect the information of the signal. For this reason, the noise removal is essential as a preprocessing process. In this paper, we propose an algorithm to remove mixed noise of impulse noise and AWGN. The proposed algorithm restores the image through noise determination and pixel change for efficient noise removal. Unlike the conventional method, noise is removed by minimizing both noise effects. Simulation showed excellent noise removal characteristic results were compared and analyzed using the PSNR for such decisions.

• Proceedings of the KOSOMBE Conference
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• v.1996 no.05
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• pp.175-178
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• 1996

The purpose of this thesis is the propose of various signal processing algorithm for the ECG(electrocardiogram) and the design of realtime automated ECG analyzer feasible with these algorithms. The algorithms are composed of (1)filtering procedure fer the estimation and removal of baseline drift, 60Hz power line interference, and muscle artifacts (2)detection procedure of QRS complex and P wave (3)typification procedure for the pattern classification according to the morphologies (4) selection of representative beat, significant point and wave boundary decision procedure and (5) parameter extraction and diagnosis procedure. All verifications are carried out between the algorithms proposed in this paper and other algorithms already proposed by many researchers, for the objective comparison in each procedure. The efficiency of proposed algorithms are demonstrated with the aid of internationally validated CSE database and the performances of filtering procedure are compared on artificial noise signal as well as actual ECG signals with appropriate noise components. for the comparison on the performance of designed automated ECG analyzer, the diagnosis results were compared with ECG analyzer manufactered by Fukuda denshi in Japan.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2001.05a
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• pp.307-310
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• 2001

A parameterized complex-number multiplier (PCMUL) core IP (Intellectual Property), which can be used as an essential arithmetic unit in baseband signal processing of digital communication systems, is described. The bit-width of the multiplier is parameterized in the range of 8-b~24-b and is user-selectable in 2-b step. The PCMUL_GEN, a core generator with GUI, generates VHDL code of a CMUL core for a specified bit-width. The IP is based on redundant binary (RB) arithmetic and a new radix4 Booth encoding/decoding scheme proposed in this paper. It results in a simplified internal structure, as well as high-speed, low-power, and area-efficient implementation. The designed IP was verified using Xilinx FPGA board.

• Journal of Haehwa Medicine
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• v.10 no.1
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• pp.365-374
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• 2001

The brain is one of the most complex systems in nature. Brain waves, or the "EEG", are electrical signals that can be recorded from the brain, either directly or through the scalp. The kind of brain wave recorded depends on the behavior of the animal, and is the visible evidence of the kind of neuronal (brain cell) processing necessary for that behavior. But, EEG had been considered as a virtually infinite-dimensional random signal. However, nonlinear dynamics light on dynamical aspects of the human EEG. The methods of nonlinear dynamics provide excellent tolls for the study of multi-variable, complex system such as EEG. In this study, 20 persons seperated in 2 groups were examined with EEG, one group stimulated on specific area of the sole of the foot with footbed inside the shoes. This experiment resulted in at the group stimulated on specific area of the sole of the foot correlation dimension of P4 and O1 channels increased significantly. Therefore. we obserbed that stimulation on specific area of the body had a constant effections on the specific channels.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.5
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• pp.614-628
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• 2019

Objective: The inhibitory leukocyte immunoglobulin-like receptors (LILRBs) play an important role in innate immunity. The present study represents the first description of the cloning and structural and functional analysis of LILRB1 and LILRB3 isolated from two genetically disparate chicken lines. Methods: Chicken LILRB1-3 genes were identified by bioinformatics approach. Expression studies were performed by transfection, quantitative polymerase chain reaction. Signal transduction was analyzed by western blots, immunoprecipitation and flow cytometric. Cytokine levels were determined by enzyme-linked immunosorbent assay. Results: Amino acid homology and phylogenetic analyses showed that the homologies of LILRB1 and LILRB3 in the chicken line 6.3 to those proteins in the chicken line 7.2 ranged between 97%-99%, while homologies between chicken and mammal proteins ranged between 13%-19%, and 13%-69%, respectively. Our findings indicate that LILRB1 and LILRB3 subdivided into two groups based on the immunoreceptor tyrosine-based inhibitory motifs (ITIM) present in the transmembrane domain. Chicken line 6.3 has two ITIM motifs of the sequence LxYxxL and SxYxxV while line 7.2 has two ITIM motifs of the sequences LxYxxL and LxYxxV. These motifs bind to SHP-2 (protein tyrosine phosphatase, non-receptor type 11) that plays a regulatory role in immune functions. Moreover, our data indicate that LILRB1 and LILRB3 associated with and activated major histocompatibility complex (MHC) class I and ${\beta}2-microglobulin$ and induced the expression of transporters associated with antigen processing, which are essential for MHC class I antigen presentation. This suggests that LILRB1 and LILRB3 are transcriptional regulators, modulating the expression of components in the MHC class I pathway and thereby regulating immune responses. Furthermore, LILRB1 and LILRB3 activated Janus kinase2/tyrosine kinase 2 (JAK2/TYK2); signal transducer and activator of transcription1/3 (STAT1/3), and suppressor of cytokine signaling 1 genes expressed in Macrophage (HD11) cells, which induced Th1, Th2, and Th17 cytokines. Conclusion: These data indicate that LILRB1 and LILRB3 are innate immune receptors associated with SHP-2, MHC class I, ${\beta}2-microglobulin$, and they activate the Janus kinase/signal transducer and activator of transcription signaling pathway. Thus, our study provides novel insights into the regulation of immunity and immunopathology.