• 제목/요약/키워드: complete genome

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퇴비에서 분리한 진세노사이드 전환능력이 있는 Niabella ginsenosidivorans BS26T 의 유전체 서열 분석 (Complete genome sequence of Niabella ginsenosidivorans BS26T, a ginsenoside-converting bacterium, isolated from compost)

  • 이영우;시디키 무하마드 주베르;류청매;김대철;임완택
    • 미생물학회지
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    • 제54권4호
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    • pp.465-467
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    • 2018
  • 퇴비로부터 분리한 Niabella ginsenosidivorans $BS26^T$ 균주의 유전체서열을 분석하였다. 균주 $BS26^T$의 유전체는 G + C 비율이 44.48%이며, 4,800개의 유전자와 4,704개의 단백질 코딩 유전자, 85개의 위유전자 그리고49개의 RNA유전자를 포함한 단일 원형 염색체로 구성되었으면 그 크기는 5,627,734 bp였다. 균주 $BS26^T$는 인삼사포닌의 당 분해에 관여하는 여러 타입의 글라이코시다제 유전자를 가지고 있었다. 이러한 유전체 분석은 주요 진세노사이드 전환에 관여하는 유전자 특징을 이해하는데 큰 기여가 되었다.

Complete Chloroplast Genome Sequence of Dumortiera hirsuta

  • Kwon, Woochan;Kim, Yongsung;Park, Jongsun
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2018년도 춘계학술발표회
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    • pp.43-43
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    • 2018
  • Dumortiera hirsuta (Sw.) Nees (Dumortieraceae) is a thallose liverwort distributed in tropics and subtropics. It is the only species in family Dumortieraceae, which is the second basal family in order Marchantiales. D. hirsuta is characterized by hairy receptacles and lacking air chamber. The complete chloroplast genome of D. hirsuta was successfully rescued from raw reads generated by HiSeq4000. Its total length is 122,050 bp consisting of four regions: large single copy (LSC) region (81,697 bp), small single copy (SSC) region (20,061 bp), and two inverted repeats (IRs; 10,146 bp per each). It contained 129 genes (84 coding DNA sequence (CDS), eight rRNAs, and 37 tRNAs); 18 genes including four rRNAs, and five tRNAs are duplicated in the IR regions. The overall GC content of D. hirsuta is 28.7%, which is almost same to that of Marchantia paleacea. Phylogenetic tree based on all genes from whole chloroplast genomes will provides phylogenetic position of D. hirstua. This sequence will be an fundamental resources for further researches of order Marchantiales.

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생물막 생성 Staphylococcus xylosus S170 균주의 유전체 분석연구 (Complete genome sequence of biofilm-producing strain Staphylococcus xylosus S170)

  • 홍지수;노은정
    • 미생물학회지
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    • 제54권2호
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    • pp.167-168
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    • 2018
  • Staphylococcus xylosus는 일반적으로 포유동물의 피부에 존재하며 식품가공설비와 의료기기 등에서도 발견이 보고되었다. 본 연구에서는 강력한 생물막 생성 특성을 가지는 Staphylococcus xylosus S170의 전체 유전체 서열을 분석하여 보고한다. 이 유전체는 2,910,005 bp 크기, 2,674개의 단백질 코딩 서열과 22개의 rRNA, 57개의 tRNA유전자를 포함한다. 본 연구에서 제공하는 유전체 정보는 생물막 관련 유전자 분석으로 생물막 형성 기작을 좀 더 잘 이해하는데 도움이 될 수 있다.

Phylogenetic position of Daphne genkwa (Thymelaeaceae) inferred from complete chloroplast data

  • YOO, Su-Chang;OH, Sang-Hun;PARK, Jongsun
    • 식물분류학회지
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    • 제51권2호
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    • pp.171-175
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    • 2021
  • Daphne genkwa (Thymelaeaceae) is a small deciduous shrub widely cultivated as an ornamental. The complete chloroplast genome of this species is presented here. The genome is 132,741 bp long and has four subregions: 85,668 bp of large single-copy and 28,365 bp of small single-copy regions are separated by 9,354 bp of inverted repeat regions with 107 genes (71 protein-coding genes, four rRNAs, and 31 tRNAs) and one pseudogene. The phylogenetic tree shows that D. genkwa is nested within Wikstroemia and is not closely related to other species of Daphne, suggesting that it should be recognized as a species of Wikstroemia.

Complete Genome Sequence of Chryseobacterium mulctrae KACC 21234T : A Potential Proteolytic and Lipolytic Bacteria Isolated from Bovine Raw Milk

  • Elnar, Arxel G.;Kim, Geun-Bae
    • Journal of Dairy Science and Biotechnology
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    • 제40권2호
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    • pp.86-91
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    • 2022
  • Chryseobacterium mulctrae KACC 21234T is a novel species isolated from raw bovine milk. Psychrotrophic bacteria are considered contaminants and are hypothesized to originate from the environment. In this investigation, the C. mulctrae KACC 21234T genome was determined to be 4,868,651 bp long and assembled into four contigs with a G+C ratio of 33.8%. In silico genomic analyses revealed the presence of genes encoding proteases (endopeptidase Clp, oligopeptidase b, carboxypeptidase) and lipases (phospholipase A(2), phospholipase C, acylglycerol lipase) that can catalyze the degradation of the proteins and lipids in milk, causing its quality to deteriorate. Additionally, antimicrobial resistance and putative bacteriocin genes were detected, potentially intensifying the pathogenicity of the strain. The genomic evidence presented highlights the need for improved screening protocols to minimize the potential contamination of milk by proteolytic and lipolytic psychrotrophic bacteria.

Discovery of Thaumaglossa rufocapillata Redtenbacher (Dermestidae, Coleoptera) from Mantis oothecae in Korea and its complete mitochondrial genome

  • Gyu-Dong Chang;Jaeil Shim;Sangmin Ji;Jeong-Hun Song
    • International Journal of Industrial Entomology and Biomaterials
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    • 제47권2호
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    • pp.115-125
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    • 2023
  • The insect industry in Korea is currently undergoing steady expansion. Among the various insects kept as pets or for educational purposes, four mantis species, including Hierodula patellifera (Audinet-Serville), Tenodera angustipennis Saussure, Tenodera sinensis Saussure, and Statilia maculata (Thunberg), are becoming increasingly popular, leading to higher demand. As we aimed to raise mantises, we collected oothecae, some of which were found to be parasitized by dermestid beetles. These parasitoids have been unequivocally identified as Thaumaglossa rufocapillata Redtenbacher based on morphological characters. A diagnosis, habitus photographs, and illustrations of diagnostic characters are provided to facilitate identification. We also present the complete mitochondrial genome (mitogenome) sequence of T. rufocapillata for providing fundamental data for research aimed at controlling pests in the insect industry.

Complete chromosome and plasmid sequences of Staphylococcus aureus strain JDFM SA01, isolated from a milk filter in Korean dairy farm

  • Ryu, Sangdon;Shin, Donghyun;Heo, Jaeyoung;Jeong, Seong-Yeop;Jeong, Do-Youn;Yun, Bohyun;Kang, Minkyoung;Kim, Younghoon;Oh, Sangnam
    • Journal of Animal Science and Technology
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    • 제62권3호
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    • pp.423-426
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    • 2020
  • Staphylococcus aureus is a significant pathogen that can source a variety of illness worldwide. In this announcement, we report here the complete genome sequence of S. aureus strain JDFM SA01, isolated from a milk filter collected from Korean dairy farm. The final complete genome assembly consists of one circular chromosome (2,748,925 bp) with an overall GC content of 32.9% and one circular plasmid sequence (24,655bp) with a GC content of 28.7%.

순환여과양식시스템으로부터 분리된 Halioglobus sp. RR3-57의 유전체 분석 (Complete genome of a denitrifying Halioglobus sp. RR3-57 isolated from a seawater recirculating aquaculture system)

  • 김영삼;노은수;이다은;김경호
    • 미생물학회지
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    • 제53권1호
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    • pp.58-60
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    • 2017
  • Halioglobus sp. RR3-57는 해수순환여과양식시스템의 생물여과조에서 순수분리되었으며, PacBio RS II 서열분석법을 이용하여 전체 유전체 서열이 해독되었다. 그 결과 길이 4,847,776 bp, G+C함량 57.5%인 염색체와 155,799 bp, 53.2%인 플라스미드로 구성된 유전체 서열을 획득하였다. 유전체 분석결과 탈질작용에 관련된 18개의 유전자와 불완전한 프로파지(prophage)로 추정되는 유전자서열이 확인되었다.

Complete nucleotide sequence of genome RNA of Daphe virus S and its relationship n the genus Carlavirus (oral)

  • Lee, B.Y.;K.H. Ryu
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.115.2-116
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    • 2003
  • Complete genomic nucleotide sequence of Daphe virus S (DVS), a member of the genus Carlavirus, causing leaf distortion and chlorotic spot disease symptoms in daphne plants, has been determined in this study. The genome of DVS contained six open reading fames coding for long viral replicase, triple gene block, 36 kDa viral coat protein (CP) and 12 kDa from the 5' to 3' ends, which is a typical genome structure of carlaviruses. Two Korean isolates of DVS isolates were 98.1% and 93.6% amino acid identical in the CP and 12kDa, respectively. The CP gene of DVS shares 25.2-55.2% and 42.9-56.1% similarities with that of 19 other carlaviruses at the amino acid and nucleotide levels, respectively. The 3'-proximal 12 kDa gene of DVS shares 20.2-57.8% amino acid identities with that of 18 other members of the genus. The 3' noncoding region of DVS consists of 73 nucleotides with long excluding poly A tract, and shares 69.1-77.1% identities to the known carlaviruses. In the phylogenetic analyses of the two proteins, DVS was closely related to Helenium virus S and Chrysanthemum virus B. This is the first complete sequence information for the DVS, and further confirms the classification of DVS as a distinct species of the genus Carlavirus.

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Bioinformatics in the Post-genome Era

  • Yu, Ung-Sik;Lee, Sung-Hoon;Kim, Young-Joo;Kim, Sang-Soo
    • BMB Reports
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    • 제37권1호
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    • pp.75-82
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    • 2004
  • Recent years saw a dramatic increase in genomic and proteomic data in public archives. Now with the complete genome sequences of human and other species in hand, detailed analyses of the genome sequences will undoubtedly improve our understanding of biological systems and at the same time require sophisticated bioinformatic tools. Here we review what computational challenges are ahead and what are the new exciting developments in this exciting field.