• Proceedings of the Korea Information Processing Society Conference
• /
• 2002.04a
• /
• pp.493-496
• /
• 2002

전자상거래에 대한 많은 관심으로 이제는 전자상거래를 통한 다양한 분야에 대한 시도도 매우 활발하게 이루어지고 있는 실정이다. 뿐만 아니라 정부와 공공기관에서도 전자상거래에 대한 다양한 지원 노력이 이루어져 그야말로 전자상거래의 신시대라 할 수 있다. 그러나 아직 많은 기업들과 단체에서는 전자상거래에 대한 도입을 주저하고 있다. 불확실한 수익구조와 미진한 기술 개발 그리고 인력 때문이다. 본 논문에서는 이러한 문제점을 해결하기 위한 수단 중 하나로서 고객관계관리(eCRM)와 공급사슬관리(eSCM)의 상호보완적 구성기법과 모델에 관하여 다루고 있으며 통합시스템을 구축하기 위한 절차와 최적의 통합시스템을 통한 비즈니스의 부가가치를 극대화하는데 목적이 있다.

• Korean Journal of Microbiology
• /
• v.27 no.3
• /
• pp.210-215
• /
• 1989

In order to elucidate and characterize the signal transduction pathway(s) whereby yeast cells respond to mating pheromone, we have isolated mutants which are able to conjugate in the absence of the alpha-factor receptor. Sixty-one suppressors of a ste2-deletion mutation which also confer a ts conditional "start" arrest phenotypw have been subjected to genetic analysis. The mutants could be assigned to three complementation groups designated CDC70, CDC72 and CDC73, which are unlinked to each other as well as to the previously identified start genes. Quantitation of mating ability of the cdc70, cdc72 and cdc73 mutations in a ste2-deletion background gives levels ranging from 0.1% to 0.3% of wild type, depending on the allele and the gene. The results indicate that the signals from mating pheromone might be mediated by the CDC70, CDC72 and CDC73 products. products.

• Environmental Mutagens and Carcinogens
• /
• v.22 no.1
• /
• pp.54-59
• /
• 2002

We have used cDNA microarray hybridization to identify gene regulated in response to gamma-irradiation in U-937 cell. The cDNA-chip was composed entirely of 1,000 human cancer related gene including apoptosis and angiogenesis etc. In gamma-irradiated U-937 cell, highly charged protein, ribosomal protein L32, four and a half LIM domains 3, lipocalin 2 (oncogene 24p3) and interleukin 15, ataxia telangiectasia mutated (includes complementation groups A, C and D) genes showed increased level of its transcription, and cell division cycle 25A, dihydrofolate reductase, topoisomerase (DNA) II beta(180kD), kinase suppressor of ras and strarigin genes showed reduced level of its transcription compared to untreated U-937 cell. The significant change of level of transcription was not found in well-known ionizing radiation(IR)-responsive gene, such as transcription factor TP53 and p53 related gene, except ataxia telangiectasia mutated gene.

• Journal of the military operations research society of Korea
• /
• v.18 no.1
• /
• pp.15-31
• /
• 1992

Developments toward a 'the end of the Cold War' are the main factor determining the situation in the Korean Peninsula. While all the combat resources of North Korea are systematically controlled and kept available for instant mobilization even in peacetime. South Korea is inferior to North Korea in terms of mobilization system and efficiency, exercise intensity and equipments. In sum, the South faces far greater restraints in wartime conversion capability than does the North. Mobilization refers to a nation's compulsory control, management and operation of its human and material resources for the purpose of national security. The total capability for safeguarding national security must be pursued by the continuous examination and complementation of our mobilization system and capability. This paper proposes an efficient management of national mobilization system that can be instantly operated on the whole in time of national emergency. Therefore, the actual operation of National Security Council is suggested to prepare operations against the emergencies and establish the foundation for national security.

• Journal of Life Science
• /
• v.10 no.2
• /
• pp.50-54
• /
• 2000

The RAD4 gene of Saccharomyces cerevisiae is essential for the incision step of UV-induced excision repair. A yeast RAD4 gene has been previously isolated by functional complementation. In order to identify the RAD4 homologous gene from fungus Coprinus cinereus, we have constructed cosmid libraries from electrophoretically separated chromosomes of the C. cinereus. The 13 C. cinereus chromosomes were resolved by pulse-field gel electrophoresis, hybridized with S. cerevisiae RAD4 DNA, and then isolated homologous C. cinereus chromosome. Here, we report the cloning and characterization of fungus C. cinereus homolog of yeast RAD4 gene. Southern blot analysis confirmed that C. cinereus contains the sequence homologous DNA to RAD4 gene and this gene exists as a single copy in C. cinereus genome. When total RNA isolated from C. cinereus cells was hybridized with the 3.4 kb BglII DNA fragment of the S. cerevisiae RAD4 gene, a 2.5 kb of transcript was detected. The isolated gene encodes a protein of 810 amino acids.

• Molecules and Cells
• /
• v.23 no.3
• /
• pp.405-409
• /
• 2007

A putative type-I chalcone isomerase (CHI) cDNA clone EuNOD-CHI was previously isolated from the root nodule of Elaeagnus umbellata [Kim et al. (2003)]. To see if it encodes a functional CHI, we ectopically overexpressed it in the Arabidopsis (Arabidopsis thaliana) transparent testa 5 (tt5) mutant, which is defective in naringenin production and has yellow seeds due to proanthocyanidin deficiency. Ectopic overexpression of EuNOD-CHI resulted in recovery of normal seed coat color. Naringenin produced by CHI from naringenin chalcone was detected in the transgenic lines like in the wild-type, whereas it was absent from the tt5 mutant. We conclude that EuNOD-CHI encodes a functional type-I CHI. In situ hybridization revealed that EuNOD-CHI expression is localized to the infected cells of the fixation zone in root nodules.

• Archives of Pharmacal Research
• /
• v.22 no.3
• /
• pp.229-231
• /
• 1999

Recessive oncogenes are genetic functions important in the regulation of tissue growth and differentiation. These genetic functions are defined on the basis of the phenotype expressed by homozygotes. Defining the role of these genes in normal developmental and physiological processes is important to the development of accurate models of the normal regulation of growth and differentiation. Drosophila can be a good system to investigate the neoplastic mechanism of oncogenes and provide a greater understanding in the developmental progression of both invertebrates and vertebrates and vertebrates. The lethal (2) giant larvae gene is a recessive oncogene of Drosophila and temperature sensitive mutations of this gene have been isolated. Here, the application of temperature-sensitive mutations in Drosophila oncogene studies is discussed.

• ALGAE
• /
• v.34 no.1
• /
• pp.35-43
• /
• 2019

Genetic study of haploid organisms offers the advantage that mutant phenotypes are directly displayed, but has the disadvantage that strains carrying lethal mutations are not readily maintained. We describe an approach for generating and performing genetic analysis of diploid strains of Chlamydomonas reinhardtii, which is normally haploid. First protocol utilizes self-mating diploid strains that will facilitate the genetic analysis of recessive lethal mutations by offering a convenient way to produce homozygous diploids in a single mating. Second protocol is designed to reduce the chance of contamination and the accumulation of spontaneous mutations for long-term storage of mutant strains. Third protocol for inducing the meiotic program is also included to produce haploid mutant strains following tetraploid genetic analysis. We discuss implication of self-fertile strains for the future of Chlamydomonas research.

• Journal of Microbiology and Biotechnology
• /
• v.32 no.8
• /
• pp.1047-1053
• /
• 2022

When ptsG, a glucose-specific phosphotransferase system (PTS) component, is deleted in Escherichia coli, growth can be severely poor because of the lack of efficient glucose transport. We discovered a new PTS transport system that could transport glucose through the growth-coupled experimental evolution of ptsG-deficient E. coli C strain under anaerobic conditions. Genome sequencing revealed mutations in agaR, which encodes a repressor of N-acetylgalactosamine (Aga) PTS expression in evolved progeny strains. RT-qPCR analysis showed that the expression of Aga PTS gene increased because of the loss-of-function of agaR. We confirmed the efficient Aga PTS-mediated glucose uptake by genetic complementation and anaerobic fermentation. We discussed the discovery of new glucose transporter in terms of different genetic backgrounds of E. coli strains, and the relationship between the pattern of mixed-acids fermentation and glucose transport rate.

• Proceedings of the Korean Society of Computer Information Conference
• /
• 2023.07a
• /
• pp.129-132
• /
• 2023

본 논문은 다양한 플랫폼에서 동일 계정으로 호환이 가능한 게임들이 늘어나고 있는 추세이다. 크로스 플랫폼을 이용하는 다양한 계층의 유저 유입을 만들어 내기 위해서 플랫폼 간의 호환 차이를 줄이는 것은 필수적이다. 플랫폼에 따른 플레이 차이는 크게 UI, 조작 밸런스 시스템 등에서 찾아 볼 수 있으며, 이를 해결하기 위해서 플랫폼에 따라 UI 디자인 재배치, 조작 차이를 줄이기 위한 밸런스 시스템 추가, 다른 플랫폼 사이의 경재 제한 등이 필수적으로 더 나은 방향에 대해서 제시해본다.