• Journal of Microbiology
• /
• 제37권4호
• /
• pp.248-255
• /
• 1999

We have analyzed the MRE3/REC114 gene of Saccharomyces cerevisiae, previously detected in isolation of mutants defective in meiotic recombination. We cloned the MRE3/REC114 gene by complementation of the meiotic recombination defect and it has been mapped to chormosome XIII. The DNA sequence analysis revealed that the MRE3 gene is identical to the REC114 gene. The upstream region of the MRE3/REC114 gene contains a T_4C site, a URS (upstream repression sequence) and a TR (T-rich) box-like sequence, which reside upstream of many meiotic genes. Coincidentally, northern blot analysis indicated that the three sizes of MRE3/REC114 transcripts, 3.4, 1.4 and 1.2 kb, are induced in meiosis. A less abundant transcript of 1.4 kb is detected in both mitotic and meiotic cells, suggesting that it is needed in mitosis as well as meiosis. To examine the role of the MRE3/REC114 gene, we constructed mre3 disruption mutants. Strains carrying an insertion or null deletion of the MRE3/REC114 gene showed slow growth in nutrient medium and the doubling time of these cells increased approximately by 2-fond compared to the wild-type strain. Moreover, the deletion mutant (${\delta}$mre3) displayed no meiotically induced recombination and no viable spores. The mre3/rec114 spore lethality can be suppressed by spo13, a mutation that causes cells to bypass reductional division. The double-stranded breaks (DSBs) which are involved in initiation of meiotic recombination were not detected in the analysis of meiotic chromosomal DNA from the mre3/rec114 disruptant. From these results we suggest that the MRE3/REC114 gene product is essential in normal growth and in early meiotic stages involved in meiotic recombination.

• 생명과학회지
• /
• 제13권4호
• /
• pp.383-389
• /
• 2003

1845 bp의 SeMIPS cDNA를 발육종자에서 분리하고 이 cDNA의 구조와 특성을 분석하였다. 이 cDNA는 엽록체로 향하는 신호펩타이드의 아미노산 서열이 존재하지 않아서 세포질형 MIPS로 예상되었다. 또한 이 cDNA의 아미노산 서열의 유사성은 다른 MIPS와 비교한 결과 60-94%의 높은 아미노산 서열 유사성을 보여주었으며, 특히 식물끼리의 유사성이 훨씬 높았다. Northern blot분석에서 볼 때 참깨의 조직별 SeMIPS mRNA는 완숙종자, 줄기, 뿌리에서는 약하게 발현되었고, 잎에서는 비교적 강하게 발현되는 현상을 보여주었다. Yeast 돌연변이체를 통한 활성 시험에서는 SeMIPS가 myo-inositol 1-phosphate synthase의 효소활성을 가지고 있다는 실험적 증거를 얻었으며, C-말단 아미노산 20개가 효소활성에 필수적이라는 사실이 본 실험에서 검증되었다.

• 생명과학회지
• /
• 제13권4호
• /
• pp.522-528
• /
• 2003

본 연구는 출아형 효모 Saccharomyces cerevisiae에서 자외선의 상해 시 이를 정상으로 회복시키는 절제회복 (excision repair) 유전자로 알려진 RADA4의 특성 규명을 위하여 균류 Coprinus cinereus에서 이와 유사한 유전자를 분리하였다. RAD4 유사 유전자를 분리하기 위하여 균류 C. cinereus의 염색체 DNA를 전기영동하여 분리한 다음 효모 RAD4 DNA를 probe로하여 이와 hybridization하였다. 이 결과 RAD4 유사 유전자는 3.2 kb의 insert DNA를 갖고 있었다. 또한 Southern hybridization으로 이 유사 유전자는 fungus C. cinereus의 염색체에 존재함을 확인하였다. 분리한 RAD4 유사 유전자의 전사체 크기는 2.5 kb 였으며, 자외선의 상해 시 전혀 'inducibility가 없음을 Northern hybridization으로 확인하였다. 또한 유사유전자 부분을 삭제하였을 때 이 부분이 없는 세포는 전혀 생존을 못하였다. 이 결과 분리한 RAD4 유사유전자는 세포의 생존에 관여함을 알 수 있었다.

• Molecules and Cells
• /
• 제38권1호
• /
• pp.81-88
• /
• 2015

Flowering time (or heading date) is controlled by intrinsic genetic programs in response to environmental cues, such as photoperiod and temperature. Rice, a facultative short-day (SD) plant, flowers early in SD and late in long-day (LD) conditions. Casein kinases (CKs) generally act as positive regulators in many signaling pathways in plants. In rice, Heading date 6 (Hd6) and Hd16 encode $CK2{\alpha}$ and CKI, respectively, and mainly function to delay flowering time. Additionally, the major LD-dependent floral repressors Hd2/Oryza sativa Pseudo-Response Regulator 37 (OsPRR37;hereafter PRR37) and Ghd7 also confer strong photoperiod sensitivity. In floral induction, Hd16 acts upstream of Ghd7 and CKI interacts with and phosphorylates Ghd7. In addition, Hd6 and Hd16 also act upstream of Hd2. However, whether CKI and $CK2{\alpha}$ directly regulate the function of PRR37 remains unclear. Here, we use in vitro pull-down and in vivo bimolecular fluorescence complementation assays to show that CKI and $CK2{\alpha}$ interact with PRR37. We further use in vitro kinase assays to show that CKI and $CK2{\alpha}$ phosphorylate different regions of PRR37. Our results indicate that direct posttranslational modification of PRR37 mediates the genetic interactions between these two protein kinases and PRR37. The significance of CK-mediated phosphorylation for PRR37 and Ghd7 function is discussed.

• 한국식생활문화학회지
• /
• 제11권1호
• /
• pp.107-121
• /
• 1996

This study was carried out to develop the computer-assisted Hazard Analysis and Critical Control Point (HACCP) program for a systematic approach to the identification, assessment and control of hazards for foodservice manager to assure the microbiological quality of food in hospital foodservice operations. Sanitation practices were surveyed and analyzed in the dietetic department of 4 hospitals. Among them, one 762-bed general hospital was selected as standard model to develop computer-assisted HACCP program. All data base files and processing programs were created by using Foxpro package for easy access of HACCP concept. HACCP program was developed based on the methods suggested by NACMCF, IAMFES and Bryan. This program consisted of two parts: the pre-stage for HACCP study and the implementation stage of the HACCP system. 1. Pre-stage for HACCP study includes the selection of menu item, the development of the HACCP recipe, the construction of a product flow diagram, and printing the HACCP recipe and a product flow diagram. A menu item for HACCP study can be selected from the menu item lists classified by cooking methods. HACCP recipe includes ingredients, their amount and cooking procedure. A flow diagram is constructed based on the HACCP recipe. The HACCP recipe and a product flow diagram are printed out. 2. Implementation of HACCP study includes the identification of microbiological hazards, the determination of critical control points, the establishment of control methods of each hazard, and the complementation of data base file. Potentially hazardous ingredients are determined and microbiological hazards are identified in each phase of the product flow. Critical control points (CCPs) are identified by applying CCP decision trees for ingredients and each process stage. After hazards and CCPs are identified, criteria, monitoring system, corrective action plan, record-keeping system and verification methods are established. When the HACCP study is complemented, HACCP study result forms are printed out. HACCP data base file can be either added, corrected or deleted.

• The Plant Pathology Journal
• /
• 제37권3호
• /
• pp.232-242
• /
• 2021

Glomerella leaf spot (GLS) is a severe infectious disease of apple whose infective area is growing gradually and thus poses a huge economic threat to the world. Different species of Colletotrichum including Colletotrichum gloeosporioides are responsible for GLS. For efficient GLS control, it is important to understand the mechanism by which the cruciferous crops and C. gloeosporioides interact. Arginine is among one of the several types of amino acids, which plays crucial role in biochemical and physiological functions of fungi. The arginine biosynthesis pathway involved in virulence among plant pathogenic fungi is poorly understood. In this study, CgCPS1 gene encoding carbamoyl phosphate synthase involved in arginine biosynthesis has been identified and inactivated experimentally. To assess the effects of CgCPS1, we knocked out CgCPS1 in C. gloeosporioides and evaluated its effects on virulence and stress tolerance. The results showed that deletion of CgCPS1 resulted in loss of pathogenicity. The ∆cgcps1 mutants showed slow growth rate, defects in appressorium formation and failed to develop lesions on apple leaves and fruits leading to loss of virulence while complementation strain (CgCPS1-C) fully restored its pathogenicity. Furthermore, mutant strains showed extreme sensitivity to high osmotic stress displaying that CgCPS1 plays a vital role in stress response. These findings suggest that CgCPS1 is major factor that mediates pathogenicity in C. gloeosporioides by encoding carbamoyl phosphate that is involved in arginine biosynthesis and conferring virulence in C. gloeosporioides.

• 한국의상디자인학회지
• /
• 제20권4호
• /
• pp.83-94
• /
• 2018

This study surveyed 255 college students enrolled in beauty contract departments located in Seoul(5 schools) and Gyeonggido(6 schools) to investigate their admission support procedure and educational satisfaction. The study found as follows; Data collection was performed from March 25 to May 30, 2018 using SPSS WIN 21.0 for their statistical processing. With respect to data analysis, first, frequency analysis was conducted to look at the general characteristics of the subjects. Second, the admission support procedure of beauty contract department was investigated and cross analysis was performed to see if there was any change according to the subjects' general characteristics. As a result of looking at the awareness on department, 89% were found to be aware of the contract department system. As for their motivation to applying for the department, the largest reasons were found to obtain a degree and to become a hair designer within a short period of time. The most frequent route of application was a recommendation by their high school teachers. The highest priority consideration in choosing a department was the brand awareness of an associated industrial entity. With respect to the complementation education in curriculum, practical technique related to the major was found the highest. 66.7% said they would recommend the department when it comes to the intention to recommend the department. 24.0% were found satisfied with the education regarding educational satisfaction. Therefore, in order to improve the educational satisfaction of the contract department, it is necessary to acquire knowledge of the field technique and practical salon education.

• 기록학연구
• /
• 제51호
• /
• pp.5-44
• /
• 2017

공공기록물은 공공행정의 효율성과 민주성을 증명하는 동시에, 국민으로 하여금 이를 확인하고 감시할 수 있도록 함으로써 알권리를 충족시켜주는 수단이다. 그러나 폐지기관은 존속기한 설정으로 인해, 생산한 기록물을 등록하지 않고 방치하는 경우가 많아 분실 위험이 크다는 지적을 받아왔다. 본 연구는 폐지기관 기록물관리의 필요성을 환기시키고, 현황파악을 통해 문제점을 도출하고 그에 따른 제시하고자 한다. 먼저 본 논문은 폐지기관 기록물관리 제도를 기관별 관리현황과 관련 법령 지침을 중심으로 분석하였다. 이를 위해 폐지기관과 국가기록원을 대상으로 기록물관리 기반 구축 현황과 관련 정책에 관한 인터뷰를 진행했고, 현행 폐지기관 기록물관리 관련 법조항과 지침사항을 살펴보았다. 그리고 분석결과를 토대로 법령 지침의 실효성 문제와 폐지기관의 기록물관리업무에 대한 인프라 구축 미흡, 국가기록원의 소극적인 대응방식 등을 주요 문제점으로 지적하였다. 본고는 이러한 문제점에 대해 관련 법령과 지침의 보완, 폐지기관 내 기록물관리업무의 전문성 강화, 유관기관간의 협의 감독 체계 구축 등 크게 세 가지 방향의 개선책을 제안하였다.

• 한국가스학회지
• /
• 제23권1호
• /
• pp.1-11
• /
• 2019

한국 정부는 액화 천연가스를 대체하는 에너지원으로서 러시아의 파이프라인 천연가스 도입을 1990년대 이후부터 지속적으로 모색해오고 있다. 이러한 동북아 에너지 협력에 대한 논의가 현실 정치뿐만 아니라 학술적인 차원에서도 다양하게 진행되고 있지만, 기존의 선행연구들은 협력의 당위성만을 제시하고 있을 뿐이지 이론적 분석틀이 미약하다는 한계를 지니고 있다. 이에 본 논문에서는 경제통합론이라는 이론적 자원을 바탕으로 에너지협력의 동기 조건 단계를 분석하고자 한다. 연구 결과 동북아 천연가스 협력과 관련해서는 경제적 동기만이 유일하기는 하지만, 실질적인 기대이익뿐만 아니라 경제구조의 보완성과 정책목표의 수렴성이라는 조건들이 갖추어진 것으로 파악되었다. 결론적으로 동북아에서 높은 수준의 에너지 협력은 어렵겠지만 특혜무역협정 수준의 초보적인 천연가스 협력이 가능하다는 전망이 제시될 수 있었다.

• 대한안전경영과학회지
• /
• 제22권4호
• /
• pp.75-86
• /
• 2020

With the advent of the 4.0 era of logistics due to the Fourth Industrial Revolution, infrastructures have been built to receive the same services online and offline. Logistics services affected by logistics 4.0 and IT technology are rapidly changing. Logistics services are developing using technologies such as big data, artificial intelligence, blockchain, Internet of things, and augmented reality. The convergence of logistics services and various IT new technologies is accelerating, and the development of data management solution technology has led to the emergence of electronic cargo waybill to replace paper cargo waybill. The electronic waybill was developed to supplement paper waybill that lack economical and safety. However, the electronic waybill that appeared to complement the paper waybill are also in need of complementation in terms of efficiency and reliability. New research is needed to ensure that electronic cargo waybill gain the trust of users and are actively utilized. To solve this problem, electronic cargo waybill that combine blockchain technology are being developed. This study aims to improve the reliability, operational efficiency and safety of blockchain electronic cargo waybill. The purpose of this study is to analyze the blockchain-based electronic cargo waybill system and to derive evaluation indicators for system supplementation.