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http://dx.doi.org/10.5352/JLS.2003.13.4.383

Characterization and functional analysis of a myo-inositol 1-phosphate synthase cDNA in sesame (Sesamum indicum L.)  

진언호 (동아대학교 응용생명공학부 생명공학과)
천재안 (동아대학교 응용생명공학부 생명공학과)
정정한 (동아대학교 응용생명공학부 생명공학과)
Publication Information
Journal of Life Science / v.13, no.4, 2003 , pp. 383-389 More about this Journal
Abstract
A cDNA (SeMIPS) encoding myo-inositol 1-phosphate synthase has been isolated from developing sesame (Sesamum indicum L. cv. Dan-Baek) seeds and its structure and function analyzed. The SeMIPS protein was highly homologous with those from plant species (88-94%), while a much lower degree of sequence homology (60%) was found with that of human. The functional domains commonly found in MIPS protein were identified and their amino acid residues were compared with each other. Northern blot indicated that the expression of the SeMIPS gene might be organ-specifically regulated. A complementation assay based on a yeast mutant system confirmed that the SeMIPS gene encodes a myo-inositol 1-phosphate synthase (MIPS) of sesame by showing functional expression of the SeMIPS cDNA in the yeast mutants containing the disrupted INO1 gene.
Keywords
Myo-inositol 1-phosphate synthase; inositol metabolism; sesame;
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