• Microbiology and Biotechnology Letters
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• v.26 no.6
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• pp.537-544
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• 1998

The cultivation conditions of transformant Alcaligenes eutrophus AER5 harboring cloned phbC gene for mass production of poly (3-hydroxybutyrate-3-hydroxyvalerate)[P(3HB-3HV)] containing high molar fraction of 3-hydroxyvalerate (3-HV) were investigated. In two-stage batch cultivation, transformant accumulated P(3HB-3HV) containing 52.2 mol% of 3HV compared to 30 mol% of parent strain A. eutrophus H16. The increased 3-HV molar fraction was due to the amplified activity of PHB synthase participating in condensation of 3-HB and 3-HV. To increase efficiency of P(3HB-3HV) accumulation, fructose was added along with precursor compound valerate, and total cell mass and P(3HB-3HV) concentrations remarkably increased, but not 3-HV molar fraction. The effect of magnesium ion showed that P(3HB-3HV) concentration and 3-HV molar fraction were significantly increased upto 6.1 g/L and 71.3 mol% at 0.01 g/L of MgSO$_4$, respectively. The efficiency of several pH adjuster, NaOH, NaOH and (NH$_4$)$_2$SO$_4$, and NH$_4$OH, on total cell mass, p(3HB-3HV) concentration, and 3-HV molar fraction was also compared. To overcome the disadvantage of two-stage cultivation, one-stage intermittent fed-batch cultivation was attempted, such a way 10.0 g/L of fructose was supplied for cell growth at initial 36 hr and then 10.0 g/L of valerate and 5.0 g/L of fructose were applied to induce the accumulation of P(3HB-3HV), consequently, 10.4 g/L of P(3HB-3HV) with 38 mol% of 3-HV fraction could be obtained after 72 hr. These results can be used for elucidating cultivation strategy for mass production of P(3HB-3HV) containing high 3-HV molar fraction using transformant A. eutrophus AER5 harboring cloned phbC gene.

• Korean Journal of Ecology and Environment
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• v.40 no.1
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• pp.130-142
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• 2007

This study was to examine impacts of turbid water on fish community in the downstream of Yongdam Dam during the period from June to October 2006. For the research, we selected six sampling sites in the field: two sites were controls with no influences of turbid water from the dam and other remaining four sites were the stations for an assessment of potential turbid effects. We evaluated integrative health conditions throughout applications of various models such as necropsy-based fish health assessment model (FHA), Index of Biological Integrity (IBI) using fish assemblages, and Qualitative Habitat Evaluation Index (QHEI). Laboratory tests on fish exposure under 400 NTU were performed to find out impact of turbid water using scanning electron microscope (SEM). Results showed that fine solid particles were clogging in the gill in the treatments, while particles were not found in the control. This results indicate that when inorganic turbidity increases abruptedly, fish may have a mechanical abrasion or respiratory blocking. The stream health condition, based on the IBI values, ranged between 38 and 48 (average: 42), indicating a "excellent" or "good" condition after the criteria of US EPA (1993). In the mean time, physical habitat condition, based on the QHEI, ranged 97 to 187 (average 154), indicating a "suboptimal condition". These biological outcomes were compared with chemical dataset: IBI values were more correlated (r=0.526, p<0.05, n=18) with QHEI rather than chemical water quality, based on turbidity (r=0.260, p>0.05, n=18). Analysis of the FHA showed that the individual health indicated "excellent condition", while QHEI showed no habitat disturbances (especially bottom substrate and embeddeness), food-web, and spawning place. Consequently, we concluded that the ecological health in downstream of Yongdam Dam was not impacted by the turbid water.

• Korean Security Journal
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• no.50
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• pp.175-206
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• 2017

The current study described the history of Islamic extremism and the recent expansion of international Islamic extremism in Indonesia. For doing so, both content analysis of the existing written documents and qualitative interviews were conducted. For the content analysis, media reports and research articles were collected and utilized. For qualitative interviews, Indonesian students and workers in Korea, Korean spouses married to Indonesian, and Korean missionaries in Indonesia were contacted and interviewed. Qualitative interview was conducted between 30 minutes and 2 hours. On the spot, interviews were recorded and later transcribed into written documents. Due to the difficulty of identification of population and the uneasiness of accessability to th study subjects, convenient sampling and snowball sampling were used. According to the results, Islamic extremism in Indonesia had a deep historical root and generally shared similar historical experience with other muslim countries such as Afghanistan, Pakistan, Egypt, and Saudi Arabia where Islamic extremism was deeply rooted in. That is, Islamic extremism began as a reaction to the western imperialism, after independence, Islamic extremism elements were marginalized in the process of construction of the modern nation-state, and Islamic extremist movement was radicalized and became violent during the Soviet-Afghan War. In addition, after 9.11, Islamic extremism in Indonesia was connected to international Islamic extremism network and integrated into such global movement. Such a historical development of Indonesian Islamic extremism was quite organized and robust. Meanwhile, the eastward infiltration and expansion of international Islamic extremism such as IS and Al Qaeda was observed in Indonesia. Particularly, such a worrisome expansion was more clearly visible in the marginalized and underdeveloped countrysides in Indonesia. Such expansion in Indonesia could negatively affect on the security of South Korea. Geographically, Indonesia is proximate to South Korea. This geographical proximity could be a direct security threat to the Korean society, as if Islamic extremism in North Africa and Middle East becomes a direct security threat to Europe. Considering the presence of a large size of Indonesian immigrant workers and communities in South Korea, such a concern is very realistic. The arrest of an Indonesian Islamic extremism supporter in November, 2016, could be a harbinger of the coming trend of Islamic extremism expansion inside South Korea. The Indonesian Islamic community in South Korea could be a passage of Indonesian Islamic extremism into the South Korean society. In this context, it is timely and necessary to pay an attention to the recent trend of Islamic extremism expansion in Indonesia.

• Korean Journal of Environment and Ecology
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• v.25 no.1
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• pp.17-30
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• 2011

This study has analyzed the vegetation composition in the areas using different slope revetment techniques of riverbanks, in a way to improve the vegetation induction in the future considering the ecological aspects of the induction in Garaeyoul Village, Gangdong-gu, Seoul. The result of the vegetation distribution in each different slope revetment technique was analyzed, that the naturalized herb was dominant in the pole block accounting for 70.7%, while native herb was dominant in the lawn block occupying 48.5% and in the crib block occupying 55.7%. The vegetation coverage measured by different slope revetment techniques was highest in the crib block(average 97.5%), followed by the lawn block(average 95.3%) and pole block(average 27.9%). The vegetation base area was the most wide in the crib block and the most narrowest in the pole block. The result of community analysis in areas using different slope revetment techniques, dry herbs and vine herbs were dominated and the coverage was very poor in the pole block. In the lawn block, the barb was fragmented into narrow areas due to the blocks, which prevented the creation of large-scale plant communities. In the crib block, Miscanthus sacchariflorus, Fescue arundinacea and Aster pilosus, which also consisted of large-scale communities. Average dry weight was also measured by each slope revetment technique. The weight was heaviest in the crib block with $187.63g/m^2$, followed by the lawn block($137.65g/m^2$) and pole block($6.75g/m^2$). The soil moisture contents in the crib block and lawn block was 16.3% and 15.2%, respectively, while that of the pole block was 4.7%, which revealed the highly dry condition of the pole block soil. The analysis result of soil chemical property showed that there was little difference in soil texture, soile acidity(pH), and exchangeable ion content, however, the soil organic matter(OM) content and available $P_2O_5$ were highest in the pole block. In the future, in order to induce more vegetation into river banks and walls, a proper construction method should be applied so that the vegetation area can be expanded as much as possible, considering the safety of the banks.

• Journal of the Korean Institute of Landscape Architecture
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• v.40 no.5
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• pp.51-62
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• 2012

Geumho-dong, Seoul, a redeveloped residential area, is located in the foothills of Mt. Eungbong. The geographical undulation, the composition of a large apartment complex, and the partial implementation of the redevelopment project have caused the severe physical and social disconnections in this area. In order to recover functioning in the disconnected community, this study pays attention to the regeneration of the open spaces as an everyday place and in the form a network system among those open spaces. Various types of the open spaces are classified into points or faces, 'bases' and linear 'paths' analyze the network status. More than half of the open space have connecting-distance of 500m or more. Furthermore, many areas are not even included in the service-area of the open spaces. Analysis of the connectivity and integration value using the axial map has carried out to check weak linkages and to choose the sections where additional bases are required. In addition, to improve the quality of the bases and the paths, a field investigation is conducted and problems are diagnosed. The network planning of the open spaces in Geumho-dong is established, ensuring the quality and quantity of bases and paths. The plan includes the construction of an additional major base in the central area and six secondary bases in other parts, and comes up with ways to improve the environment of underdeveloped secondary bases. In the neighborhood parks at Mt. Daehyun areas, the major path are added, and the environment of the paths is improved in certain areas. Because of the network planning, the connecting-distances between bases are reduced significantly, the connectivity and integration value of the area are increased, and the service areas of the open spaces cover the whole area properly. Although this study has some limitations such as the needs for the legal and institutional supports and difficulties of a quantitative indexing process, its significance lies in the suggestion of a more reasonable and practical plan for the overall network system by defining complex types of open spaces simply and clearly and by examining the organic relationships quantitatively and qualitatively.

• Journal of the Korean Institute of Landscape Architecture
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• v.41 no.5
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• pp.19-27
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• 2013

This paper claims that the One Million-peyong Park(hereafter abbreviated as OMP) project is different from a typical citizen participatory park project by recognizing the exceptional leadership of the Civic Committee for the One Million-pyeong Park Construction(CCOMPC) in promoting and developing the OMP project. Since 2001 the CCOMPC has published a variety of written promotional materials to inform and educate the public about the project. In terms of approaching the promotional materials, this research focuses on the use of language on how the CCOMPC justifies the OMP project, namely the OMP justification discourse, and considers the discourse as a unique form of social document that represents the perspective of the CCOMPC in explaining the local environmental issues and values of urban parks to the public. Using a discourse analysis method, this research analyzes the justification discourses and investigates how they changed over the three main development phases of the OMP: the initiation and preliminary development phase(1999-2001.2), the development phase (2001.2-2008), and the time period after the greenbelt policy release on Dunchi Island(2008-present). In each discourse, the OMP project is rationalized as a citizen participation park project that (1) aims to enhance the quality of public green space in Busan, (2) is accompanied by various community engagement programs that emphasize the value of urban nature and environmental education to expand citizen participation, and (3) has contributed to the National Urban Park Bill. This research emphasizes the role of the discourses in helping the public gain a critical understanding about the local environment and values of urban parks. By analyzing the contents of the discourses, it explains the social learning values of the OMP expressed in the discourses.

• Korean Journal of Ecology and Environment
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• v.45 no.1
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• pp.110-122
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• 2012

This study examined the benthic diatom community distribution, land cover/use and water quality in relation to stream shape complexity (SSC) in the Seom River watershed. SSC showed a significant relation to the riparian land cover/use pattern and also water quality variables of the studied streams. Streams with high stream shape complexity (HSC) appeared to have a high proportion of forest and farmland, while streams having a low stream shape complexity (LSC) appeared to have high proportion of city. Streams with lower SSC showed higher nutrients concentration in the stream waters. Benthic diatom species composition and dominant species appeared to be similar regardless of SSC differences among the studied streams, while the variation of diatom density was manifested with SSC. The relative abundance of dominant benthic diatoms varied with SSC. Saprophilic diatoms were dominant in the streams of LSC, while saproxenic diatoms were dominant in the streams of HSC. During the evaluation of biological water quality using the benthic diatom indices, Trophic Diatom Index (TDI) and Diatom Assemblage Index to organic water pollution (DAIpo), the streams of LSC generally showed poorer water quality than those of MSC (Middle stream shape complexity) and HSC. In particular, BOD, TP, and $PO_4$-P showed significant relationships with DAIpo. In conclusion, shape complexity of streams in the Seom River watershed showed a close relation with benthic diatom distribution. This relation seemed to primarily be resulted from the effect of proximate factors, such as water quality, which might be affected by the land use types determining the degree of SSC.

• Korean Journal of Ecology and Environment
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• v.46 no.4
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• pp.551-560
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• 2013

Environmental factors and epilithic diatom communities in the lower streams near the harbor region of South Korean peninsula were examined during no monsoon period in May 2013. The sampling of water and epilithic diatoms was conducted at both streams, 19 regulated streams (RS) that there are one or several dams constructed in the river system, and 19 un-regulated streams (US) that there are no dams within the river. A cluster analysis based on the number of species and abundance of epilithic diatoms through the stations, divided into three groups such as groups I (mainly US), II (mixed with US and RS) and III (mainly RS), respectively. Group I showed that water quality is good and high diversity of diatom, while Group II and III was water quality is relatively poor, but not differed in biomass of diatom from Group I. In addition, Group II that had high conductivity, nitrogen and phosphorus, was the lowest in diatom diversity among them. Dominant species were Nitzschia palea (17%) and Navicula seminuloides (11%) in Group I, Nitzschia inconspicua (19%) and Navicula perminuta (9%) in Group II, and Nitzschia inconspicua (15%) and Nitzschia palea (14%) in Group III, respectively. These taxa were widely distributed in brackish water, and not closely related with specific water quality, like eutrophic water. However, the groups II and III belonged to RS, had not only little biomass, but bad water quality such as high concentrations of nutrient and chlorophyll-a. Therefore, to determine the effect of dam construction on the lower water ecosystem, the planktonic algae, which can occur algal bloom in the estuary, also was considered to be a parallel investigation.

• 대한공업교육학회지
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• v.38 no.2
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• pp.89-113
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• 2013

The study started from the point that learners should be basically equipped to quickly respond to changes in demand for future human resources efficiently and effectively, and an attitude to take part in learning voluntarily should thus be cultivated. To this end, the author applied the TAI model based on a module as a class strategy to solve problems related to several classes occurred in specialized high schools. This application of the TAI model was also motivated by the necessity for designing an appropriate teaching-learning method that encourages improvement of self-learning capacity, consistent learning motivation, and willingness rather than external factors. Through this work, the author aims to heighten the interest of learners and improve endurance in learning. The study results are as follows. First, the learning unit was structuralized as a module and then learning stages were proceeded with so that capacity to adapt to learning would improve. The achievement standard (learning goal) was structuralized in a module type by analyzing and reorganizing the textbook considering the link between the actual practice field and a curriculum based on subject characteristics. Through the process of module type learning stages, the student's capacity to ascertain characteristics of units and to adapt to learning both improved. Second, through application of a TAI model based on a module, Self-Directed Learning capacity and capacity to adapt to a new field improved. By applying the module, Self-Directed Learning could be accomplished more effectively than in the existing passive learning environment. By grafting cooperative learning of the TAI model, students had greater interest and achievement in classes, self-confidence and community consciousness, and an opportunity to broaden their thinking. In particular, improvement of self-confidence is effective in cultivating the capacity required in industrial society to transcend the learning of simple functions and knowledge, as well as the capacity to adapt to new environments. Third, by solving tasks focused on fields related to industrial sites, not only actual survey capacity but also additional capacity to identify and solve tasks through various experiences improved. Furthermore, it was helpful for students to have interest and motivation about their own life and learning to cultivate the qualities and capacity to adapt to the working world where new knowledge and technology rapidly change. Fourth, by applying a standard-based assessment system for technical subjects introduced in 2012 to model design for learning plans, clearer and more objective assessment criteria were arranged, and individual learning through instruction at each achievement level could be accomplished. This improved achievement level of individual learners was helpful in reaching achievement goals at specific levels by overcoming the disadvantages of cooperative learning in each group.

• Korean Journal of Ecology and Environment
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• v.38 no.2 s.112
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• pp.196-206
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• 2005

The fish community in the mid-upper region of Gam Stream was examined seasonally from 2001 to 2003 in order to perform an environmental impact assessment prior to a construction of Gamcheon Multipurpose Dam. Additional investigation was conducted in August 2004 to confirm the fish fauna reported and to examine the ecological characteristics of Zacco temminckii population. The total number of fish caught from the study sites was 1,081 fish representing 5 families 14 species. There were 6 Korean endemic species including Coreoleuciscus splendidus, Squalidus gracilis majimae, Microphysogobio yaluensis, Liobagrus mediadiposalis, Coreoperca herzi and Odontobutis platycephala, but no endangered or vulnerable species were found. Length-weight relation, condition factor (K) and relative condition factor (Kn) of Zacco temminckii were compared by the study sites and stream. The equations based on length-weight relation in Buhang and Gam Streams were TW\;=\;0.000004TL^{3.2357}$ and TW\;=\;0.000002TL^{3.3566}$, respectively indicating the fish in Gam Stream became more rotund as length increases. The condition factor (K) and relative condition factor (Kn) against total length of Zacco temminckii at two streams indicated that the fish (>70 mm) in Cam Stream (mean K and Kn= 1.116, 1.21 respectively) had better nutritional condition than those in Buhang Stream (mean K and Ln = 1.046, 1.14 respectively). The results were corresponded with natural disturbances such as drought and intensive rainfall from 2001 to 2003 followed by human activities such as stream repair works. Therefore, it is considered to perform environmental impact assessment with not only confirmation of fish composition but also examination of ecological characteristics in population- level.