• Title/Summary/Keyword: combination culture

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Development of bag culture medium of Auricularia auricula (목이버섯 봉지재배 배지 개발)

  • Yoo, Young-Jin;Choi, So-Ra;Kim, Hee-Jun;Lee, Gi-Kwon;Song, Young-Ju;Kim, Jong-Gon
    • Journal of Mushroom
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    • v.12 no.3
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    • pp.216-219
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    • 2014
  • Studies were made to optimize the media composition in bag culture and conducted to determine the possibility of artificial cultivation of Auricularia auricula. Sawdust spawn of media composition for optimal growth were found to be oak-sawdust 80%combination of 20% popla-sawdust were the best of the optimal combination. And optimal substrate combination were found to be cotton-seed meal combination of 10% wheat bran 5% mixed were the best of combination. The duration of spawn run period and primordial formation period on bag(1.2 kg) were 50 days and 7 days, respectively. The weight of fruiting body and the yield(100ea, 1.2 kg) of fresh fruit-body were 24 g and 45,000 g, respectively.

Influence of Air Temperature and Soil Moisture Conditions on the Growth and Yield of Hot Pepper under a Plastic Tunnel Culture (고추의 비가림재배 시 온도와 토양수분 환경이 생육 및 수량에 미치는 영향)

  • Lee, Hee Ju;Lee, Sang Gyu;Choi, Chang Sun;Kim, Jun Hyeok;Kim, Sung Kyeom;Jang, Yun Ah;Lee, Sang Jung
    • Journal of Environmental Science International
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    • v.24 no.6
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    • pp.769-776
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    • 2015
  • This study was conducted to determine the effects of high temperature and deficit irrigation on growth and yield of hot pepper. Hot pepper was subjected to four irrigation treatments: fully irrigation (FI), 10, 20, and 30 days deficit irrigation (DI) combination with high temperature treatment. Control plants were grown natural environment and conventional culture methods. The plant height treated with high temperature was significantly higher than that of control plant. At FI combination with high temperature treatment, growth parameters such as stem diameter, leaf area, fresh and dry weight were the greatest. The yield was the greatest (2,036 kg/10a) under control, DI combination with high temperature treatment decreased by approximately 42% compare with FI combination with high temperature treatment. The number of abnormal fruits was approximately 38/plant under control, which was the smallest and that of 30 days DI combination with high temperature was higher 3.3 times compare with control. Flower abscission and calcium deficiency induced by DI treatments, especially those physiological disorder promoted by increasing DI treatments period. Results indicated that yield of hot pepper reduced by DI treatments, these results suggest that the growers should irrigate to proper soil moisture for preventing reduction of total fruit yield.

Efficient Plant Regeneration from Shoot Tip and Young Leaf in Rhodiola sachalinensis A. Bor.

  • Chi, Hyung-Joon;Yoon, Jae-Ho;Yang, Deok-Chun;Song, Won-Seob
    • Plant Resources
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    • v.6 no.3
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    • pp.233-241
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    • 2003
  • The shoot tip and young leaf of Rhodiola sachalinensis were cultured to invest the plant growth regulator condition for callus induction, shoot and root regeneration. When the shoot tip was sterilized in 2.0% of NaOCl for 20min., the contamination rate was the lowest. And the survival rate of the culture material was good in carbenicillin 500mg/L treatment group. Callus was obtained from shoot tip and young leaf segments. NAA 0.1-1.0mg/L and 2,4-D 0.1-0.5mg/L alone treatment were shown to have a good response on callus induction from shoot tip culture. In the case of young leaf culture, NAA and 2,4-D 0.1-0.5mg/L alone treatment were good in callus induction. In culturing shoot tip NAA 0.5mg/L and BA 0.5mg/L, NAA1.0mg/L and BA 0.lmg/L combination treatment was good in shoot regeneration. The regenerated shoots were rooted on MS medium supplemented with NAA and BA combination treatment. Especially, NAA 1.0mg/L and BA 0.1mg/L combination treatment was effective for root regeneration.

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Plant Regeneration by Anther Culture of Lilium asiatic hybrid 'Gran Paradiso' (아시아틱 백합 ( Lilium asiatic hybrid 'Gran Paradiso' )의 약배양에 의한 식물체 재생)

  • 고정애
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.1
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    • pp.1-6
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    • 1999
  • In order to obtain plantlet derived by anthers, the anthers of Lilium asiatic hybrid 'Gran Paradiso' were cultured on Murashige and Skoog's medium supplemented with various combinations of auxin and cytokinin. The most suitable pollen stage of anther culture for the callus induction was 3 days before anthesis at the early to late binucleate stage. Organogenic calli were induced on MS medium supplemented with 5.0 mg/L 2,4-D alone and the combination of 1.0 mg/L 2,4-D and 1.0 mg/L kinetin, however, the combination of NAA and BA was more effective than that of 2,4-D and kinetin on plant regeneration through organogenesis. Shoots were formed from the induced callus on the medium with 0.5 mg/L NAA and 1.0 mg/L BA after 180 days of culture. Multiple shoots with 3-4 leaves, roots, and bulblets were formed on the medium with the combination of 2.0 mg/L NAA and 2.0 mg/L BA after 250 days of culture. The chromosome from root tip of the regenerated plantlet showed the diploid (2n=2x=24). Diploid plants were transferred to the pots and all plants were flowered in two years.

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The Characteristics of Blue Color Combination Shown in Men's Fashion (남성 패션에 나타난 청색의 배색 특성)

  • Jang, Jung-Im;Cho, Ju-Yeon;Lee, Yeon-Hee
    • The Research Journal of the Costume Culture
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    • v.17 no.2
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    • pp.309-319
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    • 2009
  • This study's goal is to analyze the color characteristics of Blue used in men's fashion for design developing process. First, we researched the previous studies and examined documents about color characteristics of Blue in general as well as coloration in fashion design and men's fashion. We composed color samples by collecting two-color coloration used in men's fashion collection for 5 years from 2004 S/S to 2008 F/W through a specialized fashion information web-sites. We limited the colors from Blue Green(BG) to Purple Blue(PB). Second, we analyzed the characteristics of hue combination and tone combination. A total of 351 pictures were collected and RGB and HV/C value were converted with Munsell Conversion program(ver.8.0.1). Color data has been sorted to 10 hues and 12 PCCS tones. From this, we were able to figure out that similar/same hue coloration was used more than contrary hue coloration and similar/same tone coloration was used more than contrary tone coloration for Blue. We've limited Blue coloration characteristics of men's fashion to two-color coloration for an analysis; the succeeding study will need to examine on the characteristics of multi-coloration and detailed Blue coloration image by various garments.

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Levels of Viral Glycoprotein Provide a Measure of Modulated Chemotherapeutic Effect

  • Shin, Jaeyong;Yoon, Yeon-Sook;Pyo, Suhkneung
    • Biomolecules & Therapeutics
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    • v.7 no.3
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    • pp.216-220
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    • 1999
  • A chemosensitivity assay with small replicate Mm5mt/cl C3H mammary tumor cell cultures was developed to determine whether changes in viral antigen expression and release into culture fluids could be utilized as an in vitro measure of modulating drug effect. The 52,000 MW viral envelope glycoprotein (gp52) of the mouse mammary tumor virus (MMTV) was measured in culture fluids of control and drug-treated cultures while cell density was simultaneously determined by cell staining and OD 664 nm determination. While extra-cellular gp52 levels and cell density progressively increased over 72 hours for control cultures, declines in both parameters provided dual measures of effect for combination [N(phophonacetyl-L-aspartic acid)+5-fluorouracil], combination 〔N(phophonacetyl-L-aspartic acid )+5-fluoro-5'-deoxyuridine〕and single component treatment of this combination. At each treated time point, thesecombinations begin to produce a greater decline in both cell density and gp52 levels as compared to single drug treatments. These results indicate that N(phopho-nacetyl-L-aspartic acid) in combination can enhance the effectiveness of single drug.

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A Study on Scarf Design Development with a Combination of Traditional Korean Rice Cake Pattern and Jogakbo Patchwork (전통 떡살문양과 조각보를 조합한 스카프디자인 개발)

  • Kim, Sun-Young
    • Journal of the Korean Home Economics Association
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    • v.50 no.1
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    • pp.29-39
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    • 2012
  • The purpose of this research is to suggest a scarf design which reflects the traditional culture native to Korea by making use of the combined pattern of jokgakbo patchwork and ddeoksal(rice cake). For research methodology, the computer design programs of Adobe Illustrator CS3 and Adobe Photoshop CS3 were used along with the related literature. For the combined sets of motif pattern, the following were consider: first, a combination of jogakbo in square shape and chrysanthemum in a rice cake mold; second, a combination of radius jogakbo and geometrical pattern; third, a combination of vertical and horizontal forms of jogakbo and rice cake pattern; fourth, a combination of yeouijumunbo and butterfly pattern in rice cake mold. For the scarf design with these applications, technical skills such as repeat, rotation, symmetry, free setup, and distortion were used for the combination motif. In terms of the shape of the design, there are two, square and lengthwise tetragons.

Interspecific relationship between two food organisms in the combination culture tank of rotifer, Brachionus rotundiformis and copepod, Tigriopus japonicus (로티퍼, Brachionus rotundiformis와 코페포다, Tigsiopus japonicus의 혼합 배양조에 있어서 두 종간의 상호 관계)

  • JUNG Min-Min;RHO Sum;KIM Hyeung-Sin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.1
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    • pp.66-69
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    • 2000
  • The common harpacticoida copepod species Tigriopus japnnicus was often obseued in the mass culture of marine rotifer tanks of sea farming stations. In this study, we investigated the co-existing effect of T. iaponicus to the rotifer Brachionus rotundiformis population growth. The culture conditions as temperature, salinity, culture volume, photo period, culture period and observation interval were 25{\circ}C, 22ppt, 40ml, 12L:12D, 16 deys$ and every two days during the experimental period, respectively. Nannochloropsis oculata was used as the food for the two testing organisms. After counting the growth of rotifer and copepod, they were transferred to new culture tank with N. oculata at the density of $7{times}10^5\;cells/ml$. The population growth of B. rotundiformis was suppressed by T. japonicus when these two organisms were mix-cultured comparing to single culture of B. rotundiformis. But population growth of T, japomcus was not affected by the presence of B. rotundiformis. The interspecific relationships of prey-predation and food competition were not observed in the combination culture of rotifer B. rotundiformis and copepod T japonicus.

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The Effect of Antibiotics in Combination with EDTA-Tris on the Methicillin-Resistant Major Pathogens of Bovine Mastitis in Milk (유즙내에서 메티실린 내성을 지닌 젖소 유방염 주요 원인균에 대한 항생제와 EDTA-Tris의 병합의 효과)

  • Yoo, Jong-Hyun;Park, Hee-Myung
    • Journal of Veterinary Clinics
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    • v.25 no.5
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    • pp.346-354
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    • 2008
  • The combined effects of EDTA-Tris and eighteen antimicrobial agents have been evaluated in eight clinically isolated methicillin-resistant bacteria (Staphylococcus aureus, Escherichia coli, Streptococcus uberis and Streptococcus agalactiae) from bovine mastitis. The antimicrobial activity was evaluated by measuring the minimal bactericidal concentration (MBC) for the antibiotics alone or in combination with EDTA-Tris in Mueller-Hilton broth and milk. Combined use of EDTA-Tris and antibiotics potentiated or antagonized activity of antibiotics against mastitic pathogens. Milk increased the antibiotic potency of erythromycin and spiramycin on S. aureus. Culture in milk changed patterns of EDTA-Tris combinational effects compared with that in standard Mueller-Hilton broth. Combined with EDTA-Tris in milk, synergic effects were observed in colistin, dihydrostreptomycin, kanamycin, erythromycin, gentamycin, oxytetracycline, streptomycin to E. coli, Str. uberis, and Str. agalactiae. However, significant antagonistic effects of milk on antibiotic susceptibility in combination with EDTA-Tris were noted in neomycin, streptomycin, penicillin, roxithromycin, and amoxicillin. This study indicates that combination therapy of EDTA-Tris with antibiotics in bovine mastitis should be used with caution because of the possible antagonistic effects of antibiotic combination with EDTA-Tris on mastitic pathogens. In addition, antibiotic susceptibility test in combination with EDTA-Tris in milk culture condition can be benefit in search of effective treatment regimen for some antibiotic-resistant bacteria of mastitis.

The Effect of Combination Patterns between HRM and Business Strategy on Performance (인사관리와 사업전략 간의 결합패턴과 성과와의 관계에 대한 연구)

  • Kim, Jinhee
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.1
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    • pp.99-104
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    • 2020
  • This paper examines the performance according to the combination pattern of HRM and business strategy. The business strategy was divided into Miles & Snow's prospector, defender, and analyzer. Data were collected from the Korea Labor Institute's workplace panel survey from 2005 to 2015, and the analysis used 465 companies that are common respondents of the six wave surveys. To test the research model, structural equation modeling was employed. The results of the analysis showed that the combination of high commitment HRM and prospector had a positively significant effect on the performance. Contrariwise, the combination of high commitment HRM and defender, the combination of high commitment HRM and analyzer were not significant effect on performance.