• Title/Summary/Keyword: column method

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Residence Time Distribution in the Chromatographic Column: Applications in the Separation Engineering of DNA

  • Park, Young G.
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제8권2호
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    • pp.117-125
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    • 2003
  • Experimental and theoretical works were performed for the separation of large polyelectrolyte, such as DNA, in a column packed with gel particles under the influence of an electric field. Since DNA quickly orient in the field direction through the pores, this paper presents how intraparticle convection affects the residence time distribution of DNAs in the column. The concept is further illustrated with examples from solid -liquid systems, for example, from chromatography Showing how the column efficiency is improved by the use of a n electric field. Dimensionless transient mass balance equations were derived, taking into consideration both diffusion and electrophoretic convection. The separation criteria are theoretically studied using two different Peclet numbers in the fluid and solid phases. These criteria were experimentally verified using two different DNAs via electrophoretic mobility measurements. which showed how the separation position of the DNAs varies in the column in relation to the Peg/Pef values of an individual DNA. The residence time distribution was solved by an operator theory and the characteristic method to yield the column response.

A mathematical steel panel zone model for flanged cruciform columns

  • Saffari, Hamed;Sarfarazi, Sina;Fakhraddini, Ali
    • Steel and Composite Structures
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    • 제20권4호
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    • pp.851-867
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    • 2016
  • Cruciform sections are an appropriate option for columns of orthogonal moment resisting frames for equal bending strength and stiffness about two main axes and the implementation is easier for continuity plates. These columns consist of two I-shaped sections, so that one of them is cut out in middle and two generated T-shaped sections be welded into I-shaped profile. Furthermore, in steel moment frames, unbalance moment at the beam-column connection leads to shear deformation in panel zone. Most of the obtained relations for panel zone strength derived from experimental and analytical results are on I-shaped columns with almost thin flanges. In this paper, a parametric study has been carried out using Finite Element Method (FEM) with effective parameters at the panel zone behavior. These parameters consist of column flange thickness, column web thickness, and thickness of continuity plates. Additionally, a mathematical model has been suggested to determine strength of cruciform column panel zone and has been shown its accuracy and efficiency.

Failure analysis of reinforced concrete frames with short column effect

  • Caglar, Naci;Mutlu, Mahir
    • Computers and Concrete
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    • 제6권5호
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    • pp.403-419
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    • 2009
  • Short column effect is cause to failure of columns which may result in severe damages or even collapse during earthquakes. The scope of the study is mainly to reveal the effect of short column on the holistic behaviour of the buildings. The nonlinear analysis of 31 different frame buildings containing short column problem are carried out using finite element method. The finite element models were selected by 2 bays and 3 stories. Since the short columns are generally seen in the first storey of the buildings, in the study, they are only constructed in the same storey. The adverse effect of the short column on the response of buildings was shown in terms of the total load factor and displacement capacity of building. The response of buildings in terms of ground storey displacements is presented in figures and discussed. It is revealed that if the window openings are constructed along the bays, the total load capacity is decreased 85% compared with reference model in which all of bays are filled with infill walls.

Buckling of concrete columns retrofitted with Nano-Fiber Reinforced Polymer (NFRP)

  • Bilouei, Babak Safari;Kolahchi, Reza;Bidgoli, Mahmood Rabani
    • Computers and Concrete
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    • 제18권5호
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    • pp.1053-1063
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    • 2016
  • As concrete is most usable material in construction industry it's been required to improve its quality. Nowadays, nanotechnology offers the possibility of great advances in construction. For the first time, the nonlinear buckling of straight concrete columns armed with single-walled carbon nanotubes (SWCNTs) resting on foundation is investigated in the present study. The column is modelled with Euler-Bernoulli beam theory. The characteristics of the equivalent composite being determined using the Mori-Tanaka model. The foundation around the column is simulated with spring and shear layer. Employing nonlinear strains-displacements, energy methods and Hamilton's principal, the governing equations are derived. Differential quadrature method (DQM) is used in order to obtain the buckling load of structure. The influences of volume percent of SWCNTs, geometrical parameters, elastic foundation and boundary conditions on the buckling of column are investigated. Numerical results indicate that reinforcing the concrete column with SWCNTs, the structure becomes stiffer and the buckling load increases with respect to concrete column armed with steel.

Buckling analysis of embedded concrete columns armed with carbon nanotubes

  • Arani, Ali Jafarian;Kolahchi, Reza
    • Computers and Concrete
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    • 제17권5호
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    • pp.567-578
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    • 2016
  • As concrete is most usable material in construction industry it's been required to improve its quality. Nowadays, nanotechnology offers the possibility of great advances in construction. For the first time, the nonlinear buckling of straight concrete columns armed with single-walled carbon nanotubes (SWCNTs) resting on foundation is investigated in the present study. The column is modelled with Euler-Bernoulli and Timoshenko beam theories. The characteristics of the equivalent composite being determined using mixture rule. The foundation around the column is simulated with spring and shear layer. Employing nonlinear strains-displacements, energy methods and Hamilton's principal, the governing equations are derived. Differential quadrature method (DQM) is used in order to obtain the buckling load of structure. The influences of volume percent of SWCNTs, geometrical parameters, elastic foundation and boundary conditions on the buckling of column are investigated. Numerical results indicate that reinforcing the concrete column with SWCNTs, the structure becomes stiffer and the buckling load increases with respect to concrete column armed with steel.

콘크리트의 구속효과와 재료비선형을 고려한 내부 구속 CFT 기둥의 축력-모멘트 상호작용 분석 (Analysis of the Axial Force-Bending Moment Interaction for a CFT Column Considering the Confining Effect and the Material Nonlinearity of Concrete)

  • 한택희;염응준;윤기용;이창수;강진욱;강영종
    • 한국전산구조공학회:학술대회논문집
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    • 한국전산구조공학회 2006년도 정기 학술대회 논문집
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    • pp.141-148
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    • 2006
  • Concrete in a CFT(Concrete Filled Tube) column has enhanced strength and ductility because it is triaxially confined by a steel tube. But CFT columns are designed based on linear analyses by stress block method without the confining effect or the nonlinearity of the concrete. These make the significantly difference between the analysis results and the experimental results. Thus in this study, a nonlinear CFT column model was developed considering the confining effect on the concrete in a CFT column. This developed model was verified by experimental results from other researchers and compared with the results of various specifications. With the developed model, parametric studies were performed and the developed column model showed reasonable and accurate results.

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각형강관과 H형강보 접합부의 유한요소 모델링에 관한 연구 (An Investigation into the Finite element Modelling on connections of H-beam to S.H.S Column)

  • 이종석;변우정;이광훈;강석봉;박순규
    • 한국전산구조공학회:학술대회논문집
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    • 한국전산구조공학회 1995년도 가을 학술발표회 논문집
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    • pp.68-75
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    • 1995
  • In recent year, column member is used for square hollow section(5.H.5) and beam member is used for H-section. But 5.H.5 column has vulnerability because of low flexural stiffness between column and beam connection joints. To reinforce this vulnerability, 5.H.5 column filled with concrete and concrete slab connection compounded with H-beam is developed in many ways. In this paper, modelling of predicting behavior of various types of connections is studied using finite element method. k order to simulate the actual behavior, a three-dimensional modelling is used. A simple efficient contact algorithm with a new gap element is employed to simulate the interaction between 5.H.5 column and concrete, The modelling result$ are compared with the experimental results.

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Using the pendulum column as an isolator by reducing the gravity effect

  • Abdallah Azizi;Majid Barghian
    • Earthquakes and Structures
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    • 제25권4호
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    • pp.297-305
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    • 2023
  • The conventional method of structural seismic design was based on increasing structural capacity, which usually didn't reduce earthquake seismic effects. By changing the philosophy of structure design, technologies such as passive seismic control have been used in structures. So far, a large number of seismic isolation systems have been introduced to dissipate earthquake energy that is applied to a structure. These systems act against earthquakes rather than increasing the strength and capacity of the structure. In the present paper, a suspended column called a "pendulum column" is investigated, and a new idea has been considered to improve the performance of the pendulum column isolator by changing the gravity effect by adding a spring under the isolator system. The behavior of the studied isolator system has been researched. Then the isolator system was investigated under different earthquakes and compared with a common pendulum column isolator. The results show that changing the gravity effect has an effective role in the response of the system by reducing the system stiffness. Equations for the system showed that even in a special state, complete isolation is possible. Finally, the tested model verified the theory.

Experimental and numerical study on progressive collapse of composite steel-concrete frames

  • Jing-Xuan Wang;Ya-Jun Shen;Kan Zhou;Yong Yang
    • Steel and Composite Structures
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    • 제50권5호
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    • pp.531-548
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    • 2024
  • This paper presents an experimental investigation into the progressive collapse behavior of composite steel-concrete frames under various column removal scenarios. This study involves testing two two-bay, two-story composite frames featuring CFST columns and profiled steel decking composite slabs. Two removal scenarios, involving the corner column and middle column, are examined. The paper reports on the overall and local failure modes, vertical force-deformation responses, and strain development observed during testing. Findings indicate that structural failure initiates due to fracture and local buckling of the steel beam. Moreover, the collapse resistance and ductility of the middle column removal scenario surpass those of the corner column removal scenario. Subsequent numerical analysis reveals the significant contribution of the composite slab to collapse resistance and capacity. Additionally, it is found that horizontal boundary conditions notably influence the collapse resistance in the middle column removal scenario only. Finally, the paper proposes a simplified calculation method for collapse resistance, which yields satisfactory predictions.

Achiral/Chiral Coupled Column법에 의한 식품 중의 D-아미노산의 정량분석 (Micro-Determination of D-Amino Acids in Food by Using Achirai/Chiral Coupled Column Method)

  • 이선행;장윤희;이광필
    • 분석과학
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    • 제9권1호
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    • pp.52-61
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    • 1996
  • 식품 중의 몇몇 유리 D-아미노산이 column switching method에 의해 검출되었다. D-아미노산과 L-아미노산의 총량의 정량은 $C_{18}$ 컬럼을 사용한 비키랄 분리에 근거한다. L-아미노산에 대한 D-아미노산의 수준은 o-phthalaldehyde로 유도체화된 아미노산의 postcolumn reaction detection을 포함하는 column switching system에 의해 정량되었다. Postcolumn detection system의 키랄 분리는 chiral crown ether 컬럼에 의해 실행되었다. 이 system은 간장과 발효된 콩, 그리고 콩 같은 식품 중에 있는 D-아미노산의 정량에 적용된다. 이 achiral/chiral coupled-column system하에서는 시료 전처리과정이 중요함을 알게 되었다. Phenylalanine은 시판용 간장 속에 242ppm, 재래식 간장 속에는 102ppm, 발효된 콩에는 1g당 8.34mg, 콩에는 1g당 2.87mg이 함유된 것으로 밝혀졌다. D-phenylalanine은 시판용 간장 속에는 0.67%, 재래식 간장에는 0.34%, 발효된 콩에는 1.81% 미만, 콩에는 2.82% 미만이 검출되었다.

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