• Journal of Periodontal and Implant Science
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• 제38권1호
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• pp.41-50
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• 2008

Purpose: Bone morphogenetic protein-2(BMP-2) has been shown to possess significant osteoinducitve potential. There have been attempts to overcome a limitation of mass production, and economical efficiency of BMP. The aim of this study was to produce recombinant human BMP-2(rhBMP-2) from E. coli in a large scale and evaluate its biological activity. Materials and Methods: The E.coli strain BL21(DE3) was used as a host for rhBMP-2 production. Dimerized rhBMP-2 was purified by affinity chromatography using Heparin column. To determine the physicochemical properties of the rhBMP-2 expressed in E. coli, we examined the HPLC profile and performed Western blot analysis. The effect of the purified rhBMP-2 dimer on osteoblast differentiation was examined by alkaline phosphatase (ALP) activity and representing morphological change using C2C12 cell. Results: E. coli was genetically engineered to produce rhBMP-2 in a non-active aggregated form. We have established a method which involves refolding and purifying a folded rhBMP-2 dimer from non-active aggregates. The purified rhBMP-2 homodimer was characterized by SDS-PAGE as molecular weight of about 28kDa and eluted at 34% acetonitrile, 13.27 min(retention time) in the HPLC profile and detected at Western blot. The purified rhBMP-2 dimer stimulated ALP activity and induced the transformation from myogenic differentiation to osteogenic differentiation. Conclusion: rhBMP-2 was produced in E. coli using genetic engineering. The purified rhBMP-2 dimer stimulated ALP activity and induced the osteogenic differentiation of C2C12 cells.

• 한국세라믹학회지
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• 제44권9호
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• pp.477-482
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• 2007

The effect of the mixing ratio of compounds in a slag-OPC-Gypsum system on the physical properties of Slag cement is investigated in this study. $Na_2SO_4$ was used as an alkali activator. Blast furnace slag cement was prepared from a mixture of blast furnace slag, ordinary Portland cement and anhydride gypsum. The fluidity and the compressive strength according to the ratio of each mixture were analyzed in statistical analyses in order to discover the parameters influencing the fluidity and compressive strength. The results showed that the hydration of blast furnace slag took place with the addition of $Na_2SO_4$ and that column-crystalline ettringite was created as the main hydration product of the blast furnace slag. In addition, it was found that the compressive strength of blast furnace slag cement tends to increase when the ordinary Portland cement content is higher up to three days. However, it is known that the compressive strength tends to increase as the blast furnace slag content becomes higher with increases in the level of OPC after 28 days. As a result of this analysis, it is believed that the ordinary Portland cement content influences the initial compressive strength of blast furnace slag cement, and that in later days this is highly influenced by the slag content.

• 한국지반공학회논문집
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• 제21권5호
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• pp.215-223
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• 2005

압전 소자는 전기적 에너지를 기계적 에너지로, 기계적 에너지를 전기적 에너지로 변환하는 물질이다. 피에조 세라믹판을 이용하여 탄성 매질을 겹쳐서 만든 벤더 엘리멘트는 전압을 가하면 변위가 발생하는 특성을 이용하여 액츄에이터로 사용할 수 있고, 반대로 변위가 생기면 전압이 발생하는 압전 현상을 이용하여 변위를 계측하는 트랜스듀서로 활용하여 탄성파 시험의 발진자와 수진기로 실내 시험에서 사용되고 있다. 벤더 엘리멘트를 이용한 탄성파 시험으로 토목재료로 많이 쓰이는 콘크리트의 강성을 측정하였고, 자유단 공진주 시험을 통하여 그 결과를 평가 하였다. 카올리나이트를 이용하여 연약지반을 모사하여 카올리나이트 슬러리의 압밀에 따른 강성 변화를 탄성파 시험을 이용하여 측정할 수 있다. 벤더 엘리멘트는 연약지반에서 전단파 계측에 탁월한 성능을 가지고 있으며, 장래에 연약지반 현장 계측에 사용될 가능성이 큰 것으로 입증되었다.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2008년도 추계 학술발표회
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• pp.1463-1474
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• 2008

In order to investigate stabilization effect and sustainability on As-contaminated farmland soils which were affected the abandoned mine site and stabilized by zerovalent iron(ZVI) and industrial by-products, batch-scale and pilot-scale tests were carried out. In batch tests, ZVI and industrial by-products(blast furnace slag, steel refining slag and oyster shell powder) were used in treatment materials to reduce the As leaching. Industrial by-products were mixed with As-contaminated soils, in the ratio of 1%, 3%, 5% and 7% on the weight base of dried soil. The results of batch-scale tests was shown that the reduction of As concentration was observed in all samples and it was expected that ZVI and steel refining slag were more effective than other treatment materials to stabilize As compounds. In pilot-scale tests, columns were filled with untreated soils and treated soils mixed with ZVI and steel refining slag in the same mixing ratio of 3%. Distilled water was discharged into the columns with the velocity of 0.3 pore volume/day. During the test, pH, EC, Eh and As concentration were measured in the regular term(1pore volume). after six months, pilot-scale tests were retested to investigate sustainability of treatment materials. As a result, It was shown that the leachate from control column was continuously released during the test period and its concentration was greater than $100ug{\cdot}L^{-1}$ which was exceeded the national regulation of water discharged to river or stream ($50ug{\cdot}L^{-1}$). On the other hand, soil treated with ZVI and steel refining slag showed that the concentrations of leachate were lower than national regulation of water discharged to river or stream. Therefore it was expected that ZVI and steel refining slag could be applied to the farmland site as the alternative treatment materials.

• 전기학회논문지P
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• 제63권4호
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• pp.219-225
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• 2014

The purpose of this paper is to control of the low-speed, high-precision PMSM 2-axes pitch/turning. In this paper, apply the PAM-PWM inverter for it. However, The PAM-PWM inverter, control algorithms and hardware is complex. But it is possible to improve the performance in the low-speed operation can reduce the effect of the PWM ripple and Dead Time of inverter by applying suitable DC-bus voltage control. The direct driver PMSM(Permanent Magnet Synchronous Motor) configured to vector control part, PAM control part and the other controller. The vector control part includes PI current, speed control, additional space vector modulation. PAM control part has to have PI voltage controller and P current controller for DC-bus voltage control. Besides, the motor position estimator, the speed estimator and the counter electromotive force and Dead Time Compensation are added. With this arrangement, PMSM was driven with a low pole pitch/turning by performing the current control to the current command or torque command is the paper. As a result, it was possible to minimize the disturbance component that appears in the drive in proportion to the DC voltage magnitude. The use of a hydraulic drive method for a two-axis bubble column is a typical tank. When using the PWM PAM inverter driver is in the turret can be driven by high-precision, low vibration, low noise compared to the hydraulic drive may contribute to the computerization of the turret.

• Steel and Composite Structures
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• 제20권1호
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• pp.91-106
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• 2016

Two-dimensional elastoplastic finite element formulation is employed to investigate the load- carrying capacity degradation of reinforced concrete piers wrapped with steel plates due to occurrence of corrosion at the pier base. By comparing with experimental results, the employed finite element analysis method is verified to be accurate. After that, a series of parametric studies are conducted to investigate the effect of corrosion ratio and corrosion mode of steel plates located near the base of in-service pier P2 on load-carrying capacity of the piers. It is observed that the load-carrying capacity of the piers decreases with the increase in corrosion ratio of steel plates. There exists an obvious linear relationship between the load-carrying capacity and the corrosion ratio in the case of even corrosion mode. The degradation of load-carrying capacity resulted from the web's uneven corrosion mode is more serious than that under even corrosion mode, and the former case is more liable to occur than the latter case in actual engineering application. Finally, the failure modes of the piers under different corrosion state are discussed. It is found that the principal tensile strain of concrete and yield range of steel plates are distributed within a wide range in the case of slight corrosion, and they are concentrated on the column base when complete corrosion occurs. The findings obtained from the present study can provide a useful reference for the maintenance and strengthening of the in-service piers.

• Structural Engineering and Mechanics
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• 제39권2호
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• pp.241-266
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• 2011

The Self Compacting Concrete, SCC is the new generation type of concrete which is not needed to be compacted by vibrator and it will be compacted by its own weight. Since SCC is a new innovation and also the high strength self compacting concrete, HSSCC behavior is like a brittle material, therefore, understanding the strength effect on the serviceability performance of reinforced self compacting concretes is critical. For this aim, first the normal and high strength self compacting concrete, NSSCC and HSSCC was designed. Then, the serviceability performance of reinforced connections consisting of NSSCC and HSSCC were investigated. Twelve reinforced concrete connections (L = 3 m, b = 0.15 m, h = 0.3 m) were simulated, by this concretes, the maximum and minimum reinforcement ratios ${\rho}$ and ${\rho}^{\prime}$ (percentage of tensile and compressive steel reinforcement) are in accordance with the provision of the ACI-05 for conventional RC structures. This study was limited to the case of bending without axial load, utilizing simple connections loaded at mid span through a stub (b = 0.15 m, h = 0.3 m, L = 0.3 m) to simulate a beam-column connection. During the test, concrete and steel strains, deflections and crack widths were measured at different locations along each member. Based on the experimental readings and observations, the cracked moment of inertia ($I_{cr}$) of members was determined and the results were compared with some selective theoretical methods. Also, the flexural crack widths of the members were measured and the applicability for conventional vibrated concrete, as for ACI, BS and CSA code, was verified for SCCs members tested. A comparison between two Codes (ACI and CSA) for the theoretical values cracking moment is indicate that, irrespective of the concrete strength, for the specimens reported, the prediction values of two codes are almost equale. The experimental cracked moment of inertia $(I_{cr})_{\exp}$ is lower than its theoretical $(I_{cr})_{th}$ values, and therefore theoretically it is overestimated. Also, a general conclusion is that, by increasing the percentage of ${\rho}$, the value of $I_{cr}$ is increased.

• 한국해안해양공학회지
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• 제14권2호
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• pp.108-115
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• 2002

만경강·동진강 유역의 오염물질 유출모형을 수립·적용하였다. 모형의 입력자료인 유역정보 및 오염원 정보는 GIS도구를 이용하여 추출·분석한 자료를 이용하였으며, 월별 유출량 및 하천의 오염물질 농도 관측자료와 모형의 계산결과를 비교·분석하였다. 분석결과, 유역유출량은 여름철에 약간 과소 추정하고 있으나 전체적인 변화경향은 일치하고 있다. 또한 모형에서 계산된 오염물질 농도는 10년 평균 수질자료와 1999년도 월별 수질자료를 이용하여 비교하였다. 그 결과, 익산천에서는 크게 과소 추정하고 있으나, 전체적으로는 양호한 정도로 일치하고 있는 것으로 파악되었다 익산천에서 과소 추정하는 이유는 오염퇴적물에 의한 국지적 영향으로 사료되며, 오염퇴적물이 수층에 미치는 영향을 규명하기 위해서는 하천의 퇴적물 오염도 관측·분석을 통한 심도깊은 연구가 수행되어야 할 것이다

• 한국지진공학회논문집
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• 제15권2호
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• pp.35-42
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• 2011

현재까지 축적된 많은 연구결과와 설계기준을 바탕으로 일반적인 중실단면을 갖는 철근콘크리트 교각의 경우 큰 어려움 없이 내진설계가 수행되고 있지만, 중공원형 철근 콘크리트 교각의 경우 실험 및 해석상의 어려움으로 인하여 국내 외적으로 심부구속철근 상세에 대한 명확한 설계기준과 함께 이에 대한 합리적인 구속 모델 및 내진 성능평가 방안 등은 아직까지 미비한 실정이다. 본 연구에서는 주요 설계변수에 따른 중공원형 철근콘크리트 교각의 내진거동 특성을 파악하고, 이를 신뢰성 있는 비선형 유한요소해석 프로그램(RCAHEST)을 통한 결과와의 비교 분석을 바탕으로 보다 경제적이고 합리적인 설계방안 마련을 위한 기초자료를 제시하고자 한다.

• 한국전산구조공학회논문집
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• 제23권1호
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• pp.111-119
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• 2010

여러 연구자들에 의해 최적화 알고리즘을 이용한 최적내진설계에 관한 연구가 컴퓨터의 발달과 더불어 활발히 이루어져 왔다. 하지만 지금까지의 최적내진설계에 관한 연구는 대부분 모멘트저항골조를 대상구조물로 한 연구였다. 가새골조는 모멘트저항골조와 더불어 대표적인 횡력저항시스템이기 때문에 가새골조의 최적내진설계기법 개발을 통해 경제적이며 효율적인 설계가이드라인을 제시할 수 있다면 실무에 미치는 파급효과는 클 것이라 판단된다. 본 논문에서는 가새의 좌굴을 고려한 역V형 특수중심가새골조의 최적내진설계 알고리즘을 제안하고자 한다. 제안된 알고리즘은 구조물의 물량과 에너지 소산량을 목적함수로 설정하고, 강도조건 및 층간변위 조건등의 제약조건으로 설정한다. 알고리즘의 검증을 위해 2D 3층, 9층 역V형 특수중심가새골조 예제를 적용한다.