• 한국잡초학회지
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• 제17권4호
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• pp.375-381
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• 1997

볏단이 있던 곳이나 콤바인 수확한 볏짚이 피복된 곳은 잡초 발생인 생육이 감소하는 현상이 관찰되고 있다. 이는 볏짚에 함유한 allelopathic 물질에 의한 것으로 추정되어 볏짚을 80% 메칠알콜로 추출후 둑새풀로 생물검정, 10종의 표준 유기산이 둑새풀의 생육과 발아에 미치는 영향, 볏짚추출물을 silicagel column 으로 분획후 HPLC, GC/MS로 분석한 결과는 아래와 같았다. 1. HPLC분석에 의하여 볏짚에는 fumaric acid와 4종류의 미확인 유기산이 확인되었다. 2. fumaric acid를 포함한 7종의 유기산은 $10^{-2}$의 농도에서 둑새풀의 생육을 상당히 억제하였고 특히 발아를 100-90% 억제시켰다. 3. 볏짚에 함유한 물질중 억제효과가 가장 큰 것은 GC/MS분석에 의하여 II-4-4Rf 0.5에 함유한 물질로 분자량 253인 치환된 유기산인 신물질로 Octadecanoic acid, 2-oxo-, methyl ester에서 methyl ester가 유리된 물질로 추정되었다.

• 대기
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• 제26권1호
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• pp.73-84
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• 2016

A method to make ice spike using home refrigerator with ice tray was found. Many experiments have carried out with this method and many natural phenomena occurring on the formation of ice spike are found. A new concept of the Latter Freezing Water (LFW) was imported to explain the ice spike formation. At LFW position on water surface, the Sprout of Super cooled Water (SSW) grows by the Volume Expansion Effect (VEE) caused by the phase change of water in water. And air bubbles that are expelled from ice during freezing process, gather, rise, and detonate at the upper most part of SSW that make SSW freeze and grow upward with the water pipe in it. Together with VEE the capillarity in the water pipe makes the column grow more, that makes the ice spike. Many other findings were succeeded; 1) Ice spike process is completed before the whole water freezes. 2) If water is corrupted or shocked, even though it is very slight, ice spike is not generated. 3) Rain water contains the most LFW among all kind of waters used in experiments. 4) LFW is changed into normal water after passing the ice spike. 5) A new concept of the ice bullet is introduced. 6) The reason of frequent occurrences of the ice spike at Mt. Mai is investigated also.

• Journal of Microbiology and Biotechnology
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• 제30권11호
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• pp.1769-1776
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• 2020

As part of our current work to discover structurally and/or biologically novel compounds from Korean wild mushrooms, we isolated five ergostane-type steroids (1-5) from the fruiting bodies of Xerula furfuracea via repeated column chromatographic separations and HPLC purification. The chemical structures of the isolated steroids were shown to be (22E,24R)-24-methylcholesta-4,22-diene-3,6-dione (1), ergosta-7,22-diene-3β,5α,6β-triol (2), ergosta-7,22-diene-3β,5α,6β,9α-tetraol (3), (22E,24R)-5α,8α-epidioxyergosta-6,22-diene-3β-ol-3-O-β-D-glucopyranoside (4), and (22E,24R)-5α,8α-epidioxyergosta-6,9,22-triene-3β-ol-3-O-β-D-glucopyranoside (5)based on comparison of the data regarding their spectroscopic and physical properties with those of previous studies. Notably, this is the first report on the presence of the identified steroids (1-5) in this mushroom. We tested compounds 1-5 to determine their effects on adipogenesis and osteogenesis in the mouse mesenchymal stem cell line C3H10T1/2 and found that compounds 4 and 5 suppressed the differentiation of stem cells into adipocytes. Notably, in addition to its suppressive effect on adipogenesis, compound 5 was also shown to promote the osteogenic differentiation of stem cells. These findings demonstrate that the bioactive compounds isolated might be effective for the treatment of menopause-associated syndromes, such as osteoporosis and obesity, as the isolated compounds were shown to suppress adipogenesis and/or promote osteogenesis of stem cells.

• 한국구조물진단유지관리공학회 논문집
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• 제18권6호
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• pp.41-49
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• 2014

실험에서는 스틸밴드로 외부보강된 철근콘크리트 부재의 부착강도에 관한 연구를 진행하였으며 총 9개의 실험체를 제작하여 인발 실험을 실시하였다. 실험결과 스틸밴드의 수와 초기응력의 증가에 따라 부착강도는 증가하는 것으로 나타났다. 이것은 스틸밴드에 의해 가해지는 긴장력으로부터 철근과 콘크리트간 횡방향에 의한 구속력 증가로 판단된다. 변수로한 실험결과들을 통하여 스틸밴드로 외부보강된 철근콘크리트 부재에 관한 부착강도 산정식을 검토하였다.

• 한국식품과학회지
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• 제22권1호
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• pp.7-12
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• 1990

산화촉진제 존재하에서의 트리글리세리드 분자종의 산화특성을 밝히기 위하여, 규산 컬럼으로 분획한 대두유 트리글리세리드에 $Fe^{2+}$와 heme 화합물인 myoglobin을 첨가하여 이들 산화촉진제가 트리글리세리드 각 분자종의 신화안정성에 미치는 영향을 조사하였다. 대두유 트리글리세리드에 대한 산화촉진효과는 본 실험의 조건에서는 myoglobin이 $Fe^{2+}$보다 켰으나, 트리글리세리드 분자종의 산화안정성에 있어서는 첨가한 산화촉진제의 종류에는 큰 영향을 받지 않았다. 또 산화촉진제의 효과는 분자종의 구성지방산의 불포화도가 낮은 경우는 뚜렷하였으나 불포화도가 높은 경우는 뚜렷하지 않았다. 그리고 산화촉진제를 첨가하였을 때 트리글리세리트 분자종의 산화안정성은 분자종의 이중결합수가 같은 경우는 구성지방산의 불포화도가 낮을수록 높았으나, 구성지방산의 불포화도가 같은 경우 공존하는 포화지방산 acyl기의 사슬길이에는 영향을 받지 않았다.

• Advances in Traditional Medicine
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• 제4권4호
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• pp.267-273
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• 2004

Oxidative stress is associated with many kinds of chronic diseases. Antioxidants such as polyphenols are compounds that protect cells against the damaging effects of reactive oxygen species. Grape seeds are considered good resources of polyphenols, and grape seed extracts have a very strong antioxidant effect. In the present study, we established a simple gradient reverse-phase high performance liquid chromatography method to determine polyphenol content from three different grape seed resources. An ODS (2), $150\;{\times}\;3.2\;mm$ column has been employed, and six polyphenols have been determined: gallic acid, protochatechuic acid, (+)-catechin, (-)-epicatechin, procyanidin B2, and epicatechin gallate. Catechin and epicatechin were the main polyphenol compounds in all three extracts. The amount of procyanidin B2 was higher in Extract 1 (from a company of China), while Extract 2 (extracted in our lab) and Extract 3 (from a company of USA) contained higher proportions of epicatechin gallate. For the total polyphenol content, Extract 1 was much higher than that of Extract 2 and 3. The results suggest that the dietary dose of grape seed extracts from different resources should be adjusted according to polyphenol content.

• Journal of Acupuncture Research
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• 제18권2호
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• pp.227-236
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• 2001

Objectives : This study was performed to evaluate the clinical results of the thoracolumbar fracture patients treated with oriental medical methods. Methods : We reviewed 40 patients of thoracolumbar fracture, who were hospitalized at WonKwang University KwangJu Oriental Medical Hospital and treated by oriental medical methods. We classified thoracolumbar fracture according to three column theory and then analyzed the cause of injury, sex-age distribution, treatment, and etc.. Results : 1. The distribution showed female predominance(72.5%) in sex and 70age-bracket predominance in age. 2. In the distribution of causes, the largest group was "lifting heavy objects"(40%) and the next was "slip down"(37.5%). 3. In the duration of symptoms, the largest group was the group of "acutest" (40%). 4. The distribution of injured level was L1 body the most(20%). 5. In classification of fracture, "compression fracture" was the most(57.5%) and the next was "burst fracture", "unstable fracture" in order. 7. Therapeutic effects above "fair" were 37 cases(92.5%). 8. The higher grade of clinical symptoms is, the lower therapeutic effect is. 9. In the distribution of treatment results according to classification of fracture, both the compression fracture and the burst fracture were almost same high and the unstable fracture is relatively lower than them. Conclusions : The result to treating thoracolumbar fracture by oriental medical methods is satisfactory.

• 대한화학회지
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• 제31권1호
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• pp.45-54
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• 1987

Calcon carboxylic acid[2-hydroxy-1-(2-hydroxy-4-sulfo-1-naphthylazo)-3-naphthoic acid;CCA)]를 이온교환시킨 Dowex 1-X8수지(CCA-Dowex 1-X8)와 2-methyl-8-hydroxyquinoline(MHQ)을 침윤시킨 Amberlite XAD-4 수지(MHQ-XAD-4)를 여러가지 매질중에 있는 철(Ⅲ)이온의 분리-농축에 사용하였다. 이들 수지의 안정성을 검토하고 철(Ⅲ)이온에 대한 흡착능을 측정하였다. 매트릭수의 주성분인 Al(Ⅲ), Ca(Ⅱ), 및 Fe(Ⅲ)들의 수지에 대한 흡착성을 pH를 변화시키며 조사하여 최적 pH범위를 결정하였다. 용리법으로 알루미늄호일과 초정약수중 미량의 철분을 매트릭스 이온으로부터 분리-농축하였다. 농축된 Fe(Ⅲ)는 소량의 강산으로 용리시켜 불꽃 원자흡광광도법으로 정량하였다. 초정약수중의 Fe(Ⅱ)와 Fe(Ⅲ) 이온들은 SP-Sephadex C-25컬럼으로 농축하고, ferrozine용액과 1% ascorbic acid-ferrozine용액으로 단계적 용리법에 의해 용리시킴으로써 각각을 분리할 수 있었다. 분리된 각 이온들은 Fe(Ⅲ)-ferrozine착물의 분석 파장인 562nm에서 분광 광도법으로 정량하였다.

• 콘크리트학회논문집
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• 제22권1호
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• pp.103-111
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• 2010

미국, 유럽 그리고 일본에서는 90년대 말에 큰 지진을 경험하면서부터 지진에 대한 성능 설계 개념이 설계 기준에 포함되기 시작하였다. 일반적으로 스트럿-타이 모델은 D-영역에서 비교적 강도를 잘 예측하지만 파괴 때 연성정보를 제공하진 않는다. 그러므로 RC 구조물의 강도와 연성을 예측할 수 있는 간단한 해석 도구가 필요하다. 이 연구에서 RC 구조물의 지진에 대한 거동을 해석할 수 있는 도구인 2차원 래티스 모델을 제안한다. 2차원 래티스 모델은 실험을 통한 상관관계 연구에서 RC 구조물의 강도뿐만 아니라 연성도 예측하는 것으로 나타났다.

• The Korean Journal of Ecology
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• 제18권1호
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• pp.1-15
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• 1995

Alakline phosphatase activity (AP A) as a phosphorus deficiency measurement in flowing waters and of microhabitats (rocks, wood, leaves, and sediments) was measured and its relationship to flux of nutrients and response to rainfall events were determined for two geologically different streams in west Alabama from August to November. Results indicated water column AP A in both streams had a low correlation with levels of orthophosphate, total organic phosphorus, nitrate, ammonia, dissolved organic carbon, and discharge (r=0.075-0.583; n=g-IU. Communities on rock surfaces showed a higher AP A level than those on wood and leaves. Sediment passed through a $106{\mu}m$ sieve showed 2-9 times higher AP A level than material passed through $425{\mu}m$ sieve. The first storm after drought at Yellow Creek introduced substantial quantities of DOC (2.5 times baseflow concentrations) and $N0_3-N$ (5.8 times baseflow concentrations) which did not affect AP A significantly. The second storm at Little Schultz Creek caused minor changes in nutrient cocentrations; however $N0_3-N$ levels and AP A were drastically lower due to the dilution effect. Retention of stream water AP A at Yellow Creek and Little Schultz Creek on $0.45{\mu}m$ filter (54 and 43%, respectively) and $0.22{\mu}m$ (83 and 77% of total APA. respectively) indicated more free dissolved portion of the enzyme was present at Little Schultz Creek. Little Schultz Creek (with carbonate and with a higher productivity and biomass) showed a consistantly greater AP A activity $(132{\pm}54\;{\mu}M{\cdot}1^{-1}{\cdot}min^{-I};\;n=g)$ than Yellow Creek $(41{\pm}23\;{\mu}M{\cdot}1^{-I}{\cdot}min^{-I}$, with a sandstone substrate; n=l1, $p{\leq}O.OO1)$. Overall, a greater APA on all microhabitats and the presence of more dissolved enzyme in Little Schultz Creek during the study period may indicates it is more P deficient than Yellow Creek.