• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.489-492
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• 2002

We have performed batch chromatography experiments to calculate parameters which are used to design SMB chromatography system. Isotherms of S- and R-ketoprofen enantiomers were gained from small amount loading experiments in a column, and flow rate of four zones of SMB chromatography were calculated using mass balance equations. As a results, diameter and length of columns were 10mm and 600mm, and flow rate of each zone were as follows; $Q_{Feed}$=1.00ml/min ,$Q_{Elu}$=2.81ml/min ,$Q_{Raf}$=1.81ml/min ,$Q_{Ext}$=2.00ml/min , and $Q_{Rec}^{TMB}$=10.75ml/min , From the Darcy's equation, the pressure drop in whole columns of SMB chromatography was 128bar.

• 한국환경농학회지
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• 제15권4호
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• pp.464-471
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• 1996

Commerce silty clay loam 토양에서 제초제 trifluralin의 용탈을 조사하였다. Trifluralin의 Koc값은 875이었다. 토양 컬럼(5.4 cm i.d. ${\times}$ 26 cm length)을 이용한 용탈실험에서, 3배의 pore부피에 해당하는 물로 trifluralin을 침출 할 경우, 토양 층에는 99.993%이었고, 0.007%의 미량만 침출수로 침출 되었다. 야외포장 실험에서는 미국 Louisiana Baton Rouge에 있는 포장에 trifluralin을 1683g/ha 살포하였다. 처리 31일 후, 0-60cm 토양 층에 있는 trifluralin 전체 량의 96.9%가 0-10cm 토양 층에 분포되었다. 또한, 62일 후 1m 및 2m 지하수에서 trifluralin의 농도는 0.04-0.08ng/mL이었다. 이는 미국 EPA의 음용수 중 trifluralin의 허용농도인 2.0ng/mL보다 낮았다. Trifluralin은 토양에 강하게 흡착되었기 때문에 토양 column 및 야외 포장에서의 침출량은 매우 적었으며, 토양 column 및 야외 포장에서의 결과는 일치하였다.

• 한국공간구조학회논문집
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• 제10권1호
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• pp.95-102
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• 2010

공간구조물은 일반 라멘구조와는 다른 동적특성을 가지고 있으며 이런 동적특성에 관해 많은 연구가 수행되고 있다. 그러나 대부분의 연구는 특정 형태의 공간구조물에 대해 수행되었으며 내진설계를 위해 직접적으로 이용 가능한 연구결과는 매우 제한적이다. 본 연구에서는 공간구조물의 기본적인 동적특성을 내재한 트러스-아치구조물을 대상으로 양단의 기둥의 길이가 다른 경우에 트러스 아치구조물의 지진응답변화를 분석하고자 한다. 양단 기둥 길이의 차이에 따라 가속도 응답이 수평방향에 비해 수직방향에서 더 많은 영향을 받는다. 따라서 상부구조물을 지지하는 하부구조물의 강성이 다른 경우에 공간구조물의 내전설계에 있어서 수직방향 응답에 대한 고려가 더욱 많이 요구된다.

• Steel and Composite Structures
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• 제51권6호
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• pp.661-678
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• 2024

The utilization of geopolymer recycled aggregate concrete (GRAC) as the infilled core of the concrete-filled steel tubular (CFST) columns provides superior economic and environmental benefits. However, limited research exists within the field of geopolymer recycled aggregate concrete considered a green and sustainable material, in addition to the limitation of the design guidelines to predict the behavior of such an innovative new material combination. Moreover, the behavior of high-strength concrete is different from the normal-strength one, especially when there is another material of high-strength properties, such as the steel tube. This paper aims to investigate the behavior of the axially loaded square high-strength GRACFST columns through the nonlinear finite element analysis (NLFEA). A total of thirty-two specimens were simulated using ABAQUS/Standard software with three main variables: recycled aggregate replacement ratio (0, 30, and 50) %, width-to-thickness ratios (52.0, 32.0, 23.4, and 18.7), and length-to-width ratio (3, 5, 9, and 12). During the analysis, the response in terms of the axial load versus the longitudinal strain was recorded and plotted. In addition, various mechanical properties were calculated and analyzed. In view of the results, it has been demonstrated that the mechanical properties of high-strength GRACFST columns such as ultimate load-bearing capacity, compressive stiffness, energy absorption capacity, and ductility increase with the increase of the steel tube thickness owing to the improvement of the confinement effect of the steel tube. In contrast, the incorporation of the recycled aggregate adversely affected the mentioned properties except the ductility, while the increase of the recycled aggregate replacement ratio improved the column's ductility. Moreover, it has been found that the increase in the length-to-width ratio significantly reduced both the failure strain and the energy absorption capacity. Finally, the obtained NLFEA results of the ultimate load-bearing capacity were compared with the corresponding predicted capacities by numerous codes. It has been concluded that AISC, ACI, and EC give conservative predictions for the ultimate load-bearing capacity since the confinement effect was not considered by these codes.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2001년도 추계학술대회 논문집
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• pp.166-169
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• 2001

22,000 Ton hydraulic press was developed using wire winding method. The hydraulic press consists of three piece of frame type. The outer layers of yoke-column frame and main cylinder linear were wound with piano wire(1mm${\times}$4mm) under controlled tension and the total length of wound wire was about 450Km. The developed hydraulic press is used for the forming of heat plate with ultra-large size. To obtain large force with relative small apparatus, high pressure of $1,500 Kgf/cm^2$ was supplied to main cylinder through pressure amplification by booster pump. Therefore sealing technique of main cylinder is so crucial that the seals were made of mitre ring type with super-elastic metal. The press total weight is about 150 tons, which is quite light and compact relative to that of conventional hydraulic press.

• 한국자동차공학회논문집
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• 제16권2호
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• pp.87-92
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• 2008

The breakup processes and spray plume characteristics of liquid jets injected in subsonic air cross-flows were experimentally studied. The behaviors of column, penetration, breakup of plain liquid jet and droplet sizes, velocities have been studied in non-swirling cross-flow of air. Nozzle has a 1.0 mm diameter and Lid ratio=5. Experimental results indicate that the breakup point is delayed by increasing air momentum, the penetration decreases by increasing Weber number and the split angle is increased by increasing air velocity or decreasing injection velocity. SMD increases according as increasing height or decreases in accordance with increasing air velocity. This phenomenon is related to the momentum exchange between column waves and cross-flow stream. Droplet vector velocities were varied from 11.5 to 33 m/s. A higher-velocity region can be identified in down edge region at Z/d=40, 70 and 100. Lower-velocity region were observed on bottom position of the spray plume.

• Molecular & Cellular Toxicology
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• 제1권2호
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• pp.87-91
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• 2005

Although 2D-PAGE has been widely used as the primary method for protein separation, difficulties in displaying proteins with an extreme values of isoelectric paint (pI), molecular size and hydrophobicity limit the technique. In addition, time consuming steps involving protein transfer and extraction from the gel-pieces can result in sample loss. Here, we describe a novel protein separation technique with capillary size-exclusion chromatography (CSEC) for rapid protein identification from human plasma. The method includes protein fractionation along with molecular size followed by in-solution tryptic digestion and peptide analysis through reversed phase liquid chromatography (RPLC) coupled to nanoflow electrospray-tandem mass spectrometry (ESI-MS/MS). Tryptic peptides are applied an a $100\;{\mu}m\;i.d.{\times}10mm$ length pre-column and then separated on a $75\;{\mu}m{\times}200mm$ analytical column at -100 nL/min flaw rate. Proteins were identified over the wide ranges of pI (3.7-12.3) when this technique was applied to the analysis of $1-2\;{\mu}L$ of human plasma. This gel-free system provides fast fractionation and may be considered a complementary technique to SDS-PAGE in proteomics.

• Structural Engineering and Mechanics
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• 제6권1호
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• pp.77-93
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• 1998

Some design formulas and design procedures for single and multiple tuned liquid column dampers (TLCDs) are proposed in this study. Previous studies show that if the properties of the TLCD system are properly selected then the TLCD could be as effective as the traditional tuned mass dampers. In addition, the TLCD system offers advantages such as flexibility in terms of installation, little maintenance required, and potentials for multiple usage, etc., which are incomparable by other mechanical types of dampers. In this paper, a set of optimal properties such as length and head loss of a TLCD system are derived under the assumption that the building vibrates in a dominate mode and is subjected to Gaussian white noise excitation. A design procedure for a single TLCD system will be illustrated and discussed. Due to the nonlinearity in the damping term, the TLCD system is sensitive to the loading intensity. This loading sensitivity could limit the application range of the TLCD system. It will be shown in this paper that such a nonlinear effect can be reduced by using multiple TLCDs. As a demonstrative example, the control effects on a flexible building modeled as a single degree-of-freedom system subjected to white noise excitation will be analyzed and discussed using single or multiple TLCDs.

• 대한화학회지
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• 제39권11호
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• pp.863-868
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• 1995

내벽이 유리로 코팅된 스테인레스 스틸 관, 실리카로 코팅된 스테인레스 스틸관 및 보통 스테인레스 스틸관 등 여러 재질의 관에 C18 (5.mu.) 고정상을 충전시켜 길이 30cm, 내경 1mm 및 0.5mm인 마이크로컬럼들을 제작하고 그 성능을 비교 조사하였다.p-Nitroaniline과 N, N-dimethyl-o-nitro-p-toluidine 및 ethyl-benzene 등을 시험 용질로 사용하였다. 유리 또는 실리카로 코팅된 컬럼이 보통 스테인레스 스틸 컬럼보다 효율이 우수하며 컬럼내면이 거울같이 고와야 정지상 충전이 잘되는 것을 확인하였다. 유리코팅된 경우 내경 0.5mm, 길이 30cm 인 컬럼에 대해서도 이론단수가 10,000을 상회하는 결과를 얻을 수 있었다.

• Journal of Applied Biological Chemistry
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• 제62권1호
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• pp.73-79
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• 2019

A novel esterase gene (est7S) was cloned from an enriched metagenomic library derived from an oil-spill area. The gene encoded a protein of 505 amino acids, and the molecular mass of the Est7S was estimated to be 54,512 Da with no signal peptide. Est7S showed the highest identity of 40% to an esterase from a sludge metagenome compared to the characterized enzymes with their properties, although it showed 99% identity to a carboxylesterase in the genome sequence of Alcanivorax borkumensis SK2. Est7S had catalytic triad residues, Ser183, Glu312, and His420, and the GESAG motif in most family VII lipolytic enzymes. Est7S was purified from the crude extract of clone SM7 using Sephacryl S-200 HR and HiTrap Q column chromatographies. The purified Est7S was optimally active at $50^{\circ}C$ and pH 10.0. Est7S showed a high specific activity of 366.7 U/mg protein. It preferred short length esters, particularly p-nitrophenyl acetate, efficiently hydrolyzed R- and S-enantiomers of methyl-3-hydroxy-2-methylpropionate, and glyceryl tributyrate. These properties of Est7S may provide potential merits in biotechnological applications such as detergent and paper processing under alkaline conditions.