• 대한한방부인과학회지
• /
• 제20권1호
• /
• pp.1-15
• /
• 2007

Purpose : This study was conducted to investigate the antimicrobial effects of herb for removing dampness and promoting urination against vaginal microbes. Methods : Staphylococcus aureus. Methicillin-resistant Staphylococcus aureus(MRSA), Candida albicans and Gardnerella vaginalis were used for vaginitis-induced microbes. Lactobacillus gasseri, Streptococcus spp. and Escherichia coil HB101 were used for normal vaginal florae. And herbs for removing dampness and promoting urination(Dianthi herbs. Tokoro Rhizoma, Saururi Herbs, Pyrrosiae Folium, Artemisiae Iwayomogii Herba, Plantaginis Semen, Tetrapanacis Medulla, Polygoni Avicularis Herba, Malvae Semen, Akebiae Caulis, Kochiae Fructus, Lygodii Spora) were used. Antimicrobial activities were estimated by the change of optical densities and colony test in vitro. Results : Plantaginis Semen, Artemisiae Iwayomogii Herba and Lygodii Spora had the antimicrobial susceptibility and selective toxicity against MRSA and Gardnerella vaginalis. Polygoni Avicularis Herba had the antimicrobial susceptibility and selective toxicity against Staphylococcus aureus. MRSA and Gardnerella vaginalis. Malvae Semen and Kochiae Fructus had the antimicrobial susceptibility and selective toxicity against MRSA. Dianthi Herba had the antimicrobial susceptibility and selective toxicity against Gardnerella vaginalis. Conclusion : According to these results, we can suggest that Plantaginis Semen, Artemisiae Iwayomogii Herba, Lygodii Spora, Polygoni Avicularis Herba, Malvae Semen, Kochiae Fructus and Dianthi Herba would be available to the antimicrobial agent for vaginitis-induced microbe in vitro.

• 한국환경성돌연변이발암원학회지
• /
• 제22권2호
• /
• pp.106-111
• /
• 2002

AG6O, the complex of acriflavine and guanosine, has been shown to possess the synergistic antitumorigenic activity in the previous paper (J. Pharm. Pharmacol. 1997, 49:216). In this study, we have investigated the genotoxic properties of AG60 using in vitro and in vivo system such as Ames bacterial reversion test, chromosomal aberration assay and micronucleus assay. In Ames reverse mutation test, AG60 treatment at the dose range up to 250 $\mu\textrm{g}$/plate caused the dose-independent random induction of the mutagenic colony formation in S. typhimurium TA98, TA100, TA1537, and E. coli WP2uvrA, while any mutagenic effect of AG60 wasn't observed in S. typhimurium TA1535. Any significant chromosomal aberration wasn't observed in chinese hamster lung (CHL) fibroblast cells incubated with PBS or AG60 at the concentrations of 2.5, 5, 10 $\mu\textrm{g}$/$m\ell$ for 24 hours without but even with 59 metabolic activation system for 6 hours. In vivo ICR mice, the intramuscular injection of AG60 at the doses of 7.15, 14.3, and 28.6 mg/kg did not induce the frequency of micronucleus formation. However, mitomycin C, as one of the positive controls at the dose of 2 mg/kg caused the 8.4% induction in the frequency of micronucleus and 24% increase in the chromosomal aberration.

• 대한본초학회지
• /
• 제20권2호
• /
• pp.1-6
• /
• 2005

Objectives : The purpose of this study is to investigate genetic toxicity of Cervi pantotrichum Cornu herbal acupuncture solution(CPCHA). Methods : In this study, a series of investigation have been carried out to analyze the effects of Cervi pantotrichum Cornu herball acupuncture solution(CPCHA) on colony forming ability of NIH3T3cells, Hela cells and adrenorectal coloncell for genetic toxicity test. Results and Conclusions : From the above results, it is suggested that Cervi pantotrichum Cornu herball acupuncture solution(CPCHA) was limited 0.5-10ug/ml by test. Cervi pantotrichum Cornu herball acupuncture solution(CPCHA) did not exert the protective role to the genetic toxicity in kinds of cell lines used in this study. From these results, Cervi pantotrichum Cornu herbal aqua-acupuncture solution needs further study to prove it's function in cell culture system.

• 한국표면공학회지
• /
• 제21권2호
• /
• pp.39-46
• /
• 1988

Corrosion behavior of 5-26wt% Ni-Zn electrodeposited alloys was investigated by means of dipping electrochmical polarization measurement and galvanic coupling test. The weight loss and anodic polarization curve of coatings were measured by means of dipping and polarization measurement respectively. The galvanic protection effect of alloy coatings was measured by galvanic coupling test and the surface morpholgy of coatings was observed after corrosion testing. Our findings support thous of previous investigations showing that ${\gamma}$single phase coatings, while galvanic pprotection effect of dual phase coatings improved in comparison with ${\gamma}$single phase coating. The formation of colony in coating of ${\gamma}$and η+${\gamma}$ dual phsae caused the localized corrsion and increased the corrosion rate of the Ni-Zn coating.

• 방사선산업학회지
• /
• 제7권2_3호
• /
• pp.141-147
• /
• 2013

The simple and facile radiation technique of the preparation of antibacterial biodegradable polymer films containing silver nanoparticles (Ag NPs) was described. The biodegradable poly(butylene adipate-co-terephthalate) (PBAT) films containing silver trifluoroacetate (Ag TFA) were prepared by a solvent casting method, and then the films were irradiated by electron beams at the various doses ranging from 20 to 200 kGy to form Ag NPs in the biodegradable polymers. The results of UV-vis and FE-SEM/EDX analyses revealed that the Ag NPs were successfully formed in the PBAT matrix during the electron beam irradiation, and their amounts were dependant on the absorbed dose and Ag TFA concentrations. Furthermore, on the basis of the results of the antibacterial test through disk diffusion and colony counting test, the irradiated PBAT/Ag TFA films exhibited the antibacterial property due to the formation of Ag NPs.

• 한국콘텐츠학회논문지
• /
• 제18권3호
• /
• pp.256-264
• /
• 2018

본 연구는 구강건강행위와 구강병 발생요인검사결과와의 관련성을 확인하고자 경기도와 충청남도에 소재한 대학의 치위생과 학생을 대상으로 진행되었다. 자기기입식 설문지 작성과 구강병 발생요인검사로 진행되었으며 다음과 같은 결론을 얻었다. 칫솔질 횟수는 1일 3회가 60.2%, 1일 간식 횟수는 1회 이하가 49.7%, 간식의 종류는 점착성이 없는 가당 간식이 66.5%, 음료수의 종류는 유가당 음료수가 49.7%로 가장 높게 나타났다. 자극성 타액 분비율은 평균 9.41ml, 타액 완충능력은 평균 9.52점, 포도당 잔류시간은 평균 12.02분으로 조사되었다. Streptococcus mutans colony count 검사에서 저위험군인 <$10^5$은 80.1%, Lactobacillus는 저위험군인 <$10^5$은 82.6%로 조사되었다. 자극성 타액 분비율과 비자극성 타액 및 타액 완충능은 양의 상관관계를 보였으며, 불소도포 경험이 있는 경우 포도당 잔류시간이 10.66분, 불소도포 경험이 없는 경우 13.33분으로 나타나 통계적으로 유의한 차이를 보였다(p=.008). 전신건강과 직결되어 있는 구강건강을 위해서는 대중들이 쉽게 접할 수 있는 구강보건교육의 기회를 마련하고, 평생구강건강관리 프로그램을 지속적으로 개발 및 제공해야 할 것으로 사료된다.

• 한국환경보건학회지
• /
• 제40권3호
• /
• pp.255-263
• /
• 2014

Objectives: This study evaluated the fungicidal efficacy of a fumigant containing 20% ortho-phenylphenol against Trichophytone mentagrophytes (T. mentagrophytes), Candida albicans (C. albicans) and Aspergillus niger (A. niger). Methods: Five replicates of each carrier were contaminated by depositing 0.05 mL of each fungal suspension. After drying, two carriers without exposure to the fumigant and three carriers with exposure to the fumigant were left in a sealed room ($25m^3$) at $21{\pm}0.5^{\circ}C$ and $60{\pm}10%$ relative humidity for 15 hours. Immediately after removal from the test room, each carrier was transferred into recovery diluent and suspended, diluted and inoculated. After incubation, the numbers of each colony were counted, and the parameter values (N, T, d) were calculated. Results: The working culture suspension number (N value) of T. mentagrophytes, C. albicans and A. niger were $1.0{\times}10^8$, $1.2{\times}10^8$ and $5.7{\times}10^7CFU/mL$, respectively. All the colony numbers on the carriers exposed to the fumigant (n1, n2, n3) were higher than 0.5N1 (the number of fungal test suspensions by pour plate method), 0.5N2 (the number of fungal test suspensions by filter membrane method) and 0.5N1, respectively. In addition, all mean numbers of test strains recovered on the control-carriers (T value) were over $10^6CFU/mL$. For the fungicidal effect of the fumigant, all numbers of fungal reductions after exposure of the fumigant (d value) were 4 logCFU/mL. Conclusions: The present study showed that fumigant containing 20% ortho-phenylphenol has effective fungicidal activity against T. mentagrophytes, C. albicans and A. niger.

• 대한본초학회지
• /
• 제22권4호
• /
• pp.287-300
• /
• 2007

Objectives : The genotoxicity of extract of "Hyeonggaeyeongyotang", a polyherbal formula has been used as a tonic agents in oriental medicine was tested. Methods : Extract of "Hyeonggaeyeongyotang" was tested by In Vitro Chromosome Aberration Test, Bacterial Reverse Mutation Assay and Micronucleus test according to OECD Guidelines and KFDA Guidelines [2005-60]. Results : The obtained results were as follows: 1. Chromosome Aberration Test: No significant changes in the number of aberrant metaphases having structural and number of aberrations were detected in all concentrations of "Hyeonggaeyeongyotang" extracts treated in this study. 2. Bacterial Reverse Mutation Assay: No significant increases in the number of revertant colonies compared to its negative control were detected in all concentrations of "Hyeonggaeyeongyotang" extracts treated in this study against all 5 strains except for $50{\mu}g/ml$ treated group where significantly decreases in colony numbers were detected agains all five strains used in this study as pharmacological effects not genotoxicity. 3. Micronucleus test: No significant changes in the number of micronucleated polychromatic erythrocytes among 2000 polychromatic erythrocytes compared to negative control were detected in all "Hyeonggaeyeongyotang" extracts-dosing groups tested. Conclusions : From above-mentioned results, it is concluded that "Hyeonggaeyeongyotang" extracts have not any genotoxicity against In Vitro Chromosome Aberration Test, Bacterial Reverse Mutation Assay and Micronucleus test.

• 한국컴퓨터정보학회논문지
• /
• 제21권5호
• /
• pp.135-139
• /
• 2016

Scrophularia ningpoensis hemsl has been traditionally used in China and Vietnam for treatment of bacteria, atopy, pimple, tonsillitis, angina and encephalitis for a long time. The main objectives of this study were to evaluate the antibacterial activity of the Scrophularia ningpoensis hemsl extract on biofilm formation of Klebsiella pneumoniae. Antibacterial activity was conducted using disc diffusion assay and minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) were determined using the broth micro dilution method in accordance to Clinical and Laboratory Standards Institute guidelines(CLSI). Furthermore, cytotoxicity on L929 were assessed using animal cell culture for the proliferation test(MTT cell assay) and the biofilm forming capacity of the K. pneumoniae were determined using the colony forming unit (CFU) assay. The extract exhibited considerable antibacterial activity. K. pneumoniae was susceptible to the extract with the MIC and MBC of 0.1875 and $1.5mg/m{\ell}$ respectively. Cytoxicity test in L929 showed no sign of toxicity at the concentration of $0.75mg/m{\ell}$ and at the same concentration the extract caused inhibition of bacterial biofilm formation. The extract of Scrophularia ningpoensis hemsl possesses an in vitro antibacterial antibiofilm activities against K. pneumoniae, with no sign of cytoxicity on L929.

• KSBB Journal
• /
• 제14권2호
• /
• pp.146-152
• /
• 1999

갈반병이 유발된 느티리버섯과 비섯 재배장 주변 토양에서 채집한 균주로부터 갈반병 원인균주 4개와 갈항균주 3개를 선발하였다- 선발된 균주를 API 2DE kit에 의해 동정한 결과 갈반병 원인 균주 P-24, P-28은 Pseudomonas putida로 P-27, P-33은 P.tolaasii로 동정되었으며, 길형균주 중 A-ll, A-20은 Pseudomonas fluorescence로 A-29는 Pseudοmonas sp.로 동정되었으며, 그 중 A-ll 균주가 버섯 균사 및 자실체에 해를 주지 않으면서 길항성이 우수한 균주로 선발되었다. 길항성은 갈반병 원인균 대 길항균의 농도비를 1:1로 처리하였을 때 길항성이 양호하였다.