• Journal of Biosystems Engineering
• /
• v.28 no.5
• /
• pp.439-448
• /
• 2003

An image processing algorithm was developed for a robot system which was used in gene study. The robot system achieved a job of colony picking. The colony included DNA of an organism. The robot picked up the colony in petri-dish, which included the cultivated colony in medium, by a picking pin, and moved the colony to wellplates. The vision system consisted of an image acquisition system which acquired the image information of colony, an illumination device which irradiated the object once when it got the image of it, a computer and so on. The image processing algorithm distinguished the colony and detected colony positions. Performance test of the developed algorithm showed that the distinguishing success rate of colony and detecting success rate of colony positions were over 96%.

• The Journal of the Korean Society for Microbiology
• /
• v.21 no.3
• /
• pp.363-373
• /
• 1986

We developed a giant colony test system with rapidly growing mycobacteria by stab-culture with a loopful inoculum of cells into Middlebrook 7H10 agar medium containing soluble extracts of the fruits of Gardenia jasminoides(7H10-crocin agar medium) and assessed the significance of the giant colony test with 28 strains of 10 clusters of rapidly growing mycobacteria classified by the simple biological 5-test characters. Of the 10 clusters of mycobacteria tested, some of strains which belonged to cluster No. 1a, 5a and 11a did grow as gravis types, whereas most of other clusters gave mitis or intermedius types in their colonial sizes at 12 days culture. By this test, pathogenic strains of M. fortuitum-chelonei complex which belonged to cluster No. 5a, b, 7a and 8a, b could be divided into gravis, intermedius and mitis colony types and the gravis ones were characterized by bluish-white "mushroom-shaped" colonies with central complexes in the texture, whereas the intermedius gave grayish-white "flower-shaped" colonies with radiated folds, but without any central complexes. The mitis colonies were characterized by grayish-white smooth or smooth mucoid colonies and were common among the clusters in their shapes. The colony of M. chelonei was bluish-white mitis type and was characterized by its hilly rhizoid colony. The gravis colony of cluster No. 1a identified as M. phlei was characterized by yellow "round straw- mat-shaped" or "chrysanthemum-shaped" colony with whole complexes in the texture, and the gravis colonies of the cluster No. 11a gave grayish-white "flower-shaped" colonies with central stamens, radiating trough and fine cup-shaped strands in the texture. The four colony types of pathogenic species of M. fortuitum-chelonei complex on 7H10-crocin agar medium were distinctive from those of other clusters of rapidly growing mycobacteria and these results indicated that the giant colony test, in conjunction with the simple 5-test characters, would be of value in the differentiation of M. fortuitum complex from other clusters of rapidly growing mycobacteria.

• Journal of Biosystems Engineering
• /
• v.28 no.5
• /
• pp.449-456
• /
• 2003

A robot system was developed to pick desired colony. This robot system consisted of an image acquisition process which acquires the image information of colony, an illumination device which irradiates the object once when it gets the image of it, a picking head, a replicating head, a bed for fixing well-plates, and a sterilization device of sterilizing pin stained with colony. Picking pins were washed in an ultrasonic wave washing for takes ten seconds. Picking pins were dipped for sterilizing in alcohol for ten seconds. The time for resterilizing picking pins in a heater was five seconds. This performance test resulted 100% success rate for both the colony picking and the colony replication process. Considering the procedure in which 8 pins were smeared by colony, picked on a well plate, and sterilized as one cycle, the system could carry out one cycle in 110 seconds. It took about 138 minutes to pick around 600 colonies.

• ETRI Journal
• /
• v.30 no.1
• /
• pp.129-140
• /
• 2008

In this paper, we propose a novel ant colony optimization (ACO)-based test scheduling method for testing network-on-chip (NoC)-based systems-on-chip (SoCs), on the assumption that the test platform, including specific methods and configurations such as test packet routing, generation, and absorption, is installed. The ACO metaheuristic model, inspired by the ant's foraging behavior, can autonomously find better results by exploring more solution space. The proposed method efficiently combines the rectangle packing method with ACO and improves the scheduling results by dynamically choosing the test-access-mechanism widths for cores and changing the testing orders. The power dissipation and variable test clock mode are also considered. Experimental results using ITC'02 benchmark circuits show that the proposed algorithm can efficiently reduce overall test time. Moreover, the computation time of the algorithm is less than a few seconds in most cases.

• 보존과학연구
• /
• s.23
• /
• pp.77-93
• /
• 2002

There are several methods of pest control for the prevention of wooden cultural properties from damages caused by insects, especially termites. Those are as follows; physical control, chemical control, biological control, and integrated pest management. In Korea, we primarily use mvestigation of monitoring, fumigation, treatment of insecticidal and antiseptic chemicals, soil termiticide treatment, and termite colony elimination system. As damages by termites were detected at Eunghyanggak and Saundang of Haeinsa temple in June, 1998, we carried out monitoring with wooden device, in order to investigate damages of wooden building by termite and paths of termite invasion. According to the result of investigation of monitoring from1999 to 2002, we found that termites were inhabiting continually at forests around Haeinsa and that wooden buildings such as Saundang, Cheonghwadang were damaged by termites. So we thought that control methods such as soil termiticide treatment and termite colony elimination system would be effective. Especially, to investigate efficacy of termite colony elimination system, we carried out efficacy test of termite colony elimination system in Jongmyo during 17 months(2000.7.~2001.11.). The result of efficacy test showed that termite colony elimination system eliminated termite colonies gradually(over 12 months from installation of colony elimination system to elimination of termite colony).

• Animal Systematics, Evolution and Diversity
• /
• v.40 no.1
• /
• pp.102-107
• /
• 2024

Two colonial ascidians, Eudistoma glaucum and Eudistoma purpureum, are reported for the first time in Korean waters through taxonomic study on ascidians collected from a subtidal zone of Jejudo Island. Eudistoma glaucum is distinguished by opaque green color of colony in living, massive colony with large corona, smooth surface of corona, sparse sand only at the peduncle, zooids in circle, about 8-10 stigmata of 3 stigmata rows and test process. Eudistoma purpureum is distinguished by brilliant, opaque, purple color of colony in living, less lobed colony form, smooth shiny surface, sparse sand only at the basal test, absence of symbionts, zooids in circle, no distinct bulging sphincter in siphon, long atrial siphon and about 20 stigmata of 3 stigmata rows. As a result of this study, four species of the genus Eudistoma are now recorded in Korean fauna.

• Toxicological Research
• /
• v.9 no.1
• /
• pp.69-72
• /
• 1993

LBD-005, a newly developed recombinant granulocyte-macrophage colony stimulating factor, was tested for primary skin irritation in male New Zealand White rabbits. In the primary skin irritation test, LBD-005 was applied to intact and abraded skins for 24 hours. Primary irritation index was "0" in test and control sites of all animals' thus LBD-005 was evaluated as a non-irritatant on the basis of the criteria of Draize et al. (1994).l. (1994).

• The Journal of Korean Obstetrics and Gynecology
• /
• v.20 no.2
• /
• pp.124-138
• /
• 2007

Purpose: This study was conducted to investigate the antimicrobial effects of the interior warming herbs on vaginal microbes. Methods: Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Candida albicans and Gardnerella vaginalis were used for vaginitis-induced microbes. Lactobacillus gasseri, Streptococcus spp. and Escherichia coli HB101 were used for normal vaginal florae. And herbs for warming the interior (Zingiberis Rhizoma, Alpiniae Officinari Rhizoma, Aconiti Tuber, Anethi Fructus, Evodiae Fructus, Cinnamomi Cortex Spissus, Caryophylli Flos, Aconiti Tube, Zanthoxyli Pericarpium, Piperis Longi Fructus, Piperis Nigri Fructus) were used in this study. Antimicrobial activities were tested by the change of optical densities (OD) and colony test in vitro. Results: In the results of vaginitis-induced microbes, Anethi Fructus was decreased the OD values on MRSA and Gardnerella vaginalis and Aconiti Tuber was decreased on MRSA. There were no viable MRSA and Gardnerella vaginalis colony forming against Evodiae Fructus, Staphylococcus aureus and MRSA colony forming against Piperis Longi Fructus, Staphylococcus aureus colony forming against Piperis Nigri Fructus and MRSA colony forming against Zanthoxyli Pericarpium. In the results of normal vaginal florae, Zingiberis Rhizoma was decreased the OD values on Streptococcus spp. and all normal vaginal florae were showed viable colony forming against all experimental herbs. Conclusion: According to these results, we can suggest that some kinds of interior warming herbs have antimicrobial effects on vaginal microbes. So there might be needed to make furthermore studies to seek the herbs which have selective antimicrobial effect on pathologic vaginal microbes.

• Proceeding of EDISON Challenge
• /
• 2014.03a
• /
• pp.63-74
• /
• 2014

Predicting protein loop structures is an important modeling problem since protein loops are often involved in diverse biological functions by participating in enzyme active sites, ligand binding sites, etc. However, loop structure prediction is difficult even when structures of homologous proteins are known due to large sequence and structure variability among loops of homologous proteins. Therefore, an ab initio approach is necessary to solve loop modeling problems. One of the difficulties in the development of ab initio loop modeling method is to derive an accurate scoring function that closely approximates the true free energy function. In particular, entropy as well as energy contribution have to be considered adequately for loops because loops tend to be flexible compared to other parts of protein. In this study, the contribution of conformational entropy is considered in scoring loop conformations by employing "colony energy" which was previously proposed to estimate the free energy for an ensemble of conformations. Loop conformations were generated by using two EDISON_Chem programs GalaxyFill and GalaxySC, and colony energy was designed for this sampling by tuning relevant parameters. On a test set of 40 loops, the accuracy of predicted loop structure improved on average by scoring with the colony energy compared to scoring by energy alone. In addition, high correlation between colony energy and deviation from the native structure suggested that more extensive sampling can further improve the prediction accuracy. In another test on 6 ligand-binding loops that show conformational changes by ligand binding, both ligand-free and ligand-bound states could be identified by using colony energy when no information on the ligand-bound conformation is used.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.28 no.1
• /
• pp.16-24
• /
• 2001

The purpose of this study was to evaluate the specificity, sensitivity, and diagnostic power of caries activity test using laser fluorescence. The subjects of this study were 50 children of $7\sim9$ years old. Fluorescence from initial carious lesion of teeth illuminated by an argon laser(480nm) was observed and photographed with barrier filter. Visual examination for the dDfFtT rate and Streptococcus mutans colony counting was done to evaluate correlation with caries activity test using laser fluorescence. Data analysis was accomplished by Axelsson's method. The results from the present study can be summarized as follows: 1. There was positive correlation $(\gamma=0.48)$ between laser fluorescence test and Streptococcus mutans count. And also positive correlation $(\gamma=0.39)$ exists between laser fluorescence test and dDfFtT rate (P<0.01). 2. Positive correlation $(\gamma=0.27)$ between Streptococcus mutans colony count and dDfFtT rate was found(P<0.05). 3. When dDfFtT rate was defined to standard testing method, the specificity, senstivity, and diagnostic power of laser fluorescence test were 44.4%, 85.7%, and 87.8%. 4. When dDfFtT rate was defined to standard testing method, the specificity, senstivity, and diagnostic power of S. mutans colony counting were 77.8%, 92.9%, 84.8%. 5. When S. mutans colony counting was defined to standard testing method, sensitivity, specificity and diagnostic power of laser fluorescence test were 40.0%, 84.8%, 95.1%. In regard to above results, laser fluorescence test considered to be accurate and reliable method for determining caries activity because of it's close relationship with caries susceptibility test and caries experience measurements. And it was also considered to be practical because it would be simple, inexpensive, and time saving method.