• Journal of Pharmaceutical Investigation
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• v.31 no.4
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• pp.241-256
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• 2001

Alginate microspheres, containing fluorescein isothiocyanate-bovine serum albumin (FITC-BSA) or green fluorescent protein (GFP) were prepared and used as a model drug to develop the oral vaccine delivery system. The alginate microspheres were coated with poly-L-lysine or chitosan. Two methods, w/o-emulsion and spray, were used to prepare alginate microspheres. To optimize preparation conditions, effects of several factors on the particle size and particle morphology of microsphere, and loading efficiency of model antigen were investigated. In both preparation methods, the particle size and the loading efficiency were enhanced when the concentration of sodium alginate increased. In the w/o-emulsion preparation method, as the concentration of Span 80 was increased from 0.5% to 2%, the particle size was decreased, but the loading efficiency was increased. The higher the emulsification speed was, the smaller the particle size and loading efficiency were. The concentration of calcium chloride did not show any effect on the particle size and loading efficiency. In the spray preparation method, the particle size was increased as the nozzle pressure $(from\;1\;kgf/m^2\;to\;3\;kgf/m^2)$ and spray rate was raised. Increasing calcium chloride concentration (<7%) decreased the particle size, in contrast to no effect of calcium chloride concentration on the w/o-emulsion preparation method. Alginate microspheres prepared by two methods were different in the particle size and loading efficiency, the particle size of microspheres prepared by the spray method was about $2-6\;{\mu}m$, larger than that prepared by the w/o emulsion method $(about\;2{\mu}m)$, and the loading efficiency was also higher with spray method. Furthermore, drying process for the microspheres prepared by the spray was simpler and easier, compared with the w/o emulsion preparation. Therefore, the spray method was chosen to prepare alginate microspheres for further experiments. Release pattern of FITC-BSA in alginate microspheres was evaluated in simulated intestinal fluid and PBS (phosphate buffered saline). Dissolution rate of FITC-BSA from alginate/chitosan microsphere was lower than that from alginate microsphere and alginate/poly-L-lysine microsphere. By confocal laser scanning microscope, it was revealed that alginate/FITC-poly-L-lysine microspheres were present in close apposition epithelium of the Peyer's patches of rabbits following inoculation into lumen of intestine, which proved that microspheres could be taken up by Peyer's patch. In conclusion, it is suggested that alginate microsphere prepared by spray method, showing a particle size of & $10\;{\mu}m$ and a high loading efficiency, can be used as a model drug for the development of oral vaccine delivery system.

• The Journal of the Convergence on Culture Technology
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• v.9 no.3
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• pp.67-74
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• 2023

The purpose of this study is to find relationships between the moral distress of nursing college students and career maturity, and between the moral distress of them and career preparation behavior by using a descriptive correlation methodology. Analysis is done using t-test, ANOVA, Pearson correlation coefficient, and Multiple regression with IBM SPSS WIN/22.0. According to the general characteristics, the there is a significant difference in the subjects with an outgoing personality (F=-2.82, p=.001), who suffered from moral distress at an older age (F=.4.85, p=.029). There is a significant difference in career maturity among subjects with an extroverted personality (F=3.63, p=.001). The average score of the subjects' moral distress is 72.51±53.75, and by sub-area, futile care was the highest with 23.36±19.04, and the lowest with 7.61±8.56 in physician practice. The average of career maturity was 3.74(±.56), and by sub-area, career attitude was the highest with 3.98±.54, and the lowest with 3.61±.43 in professionalism. The average of career preparation behavior was 2.67(±.60). In this study, it was confirmed that the moral stress of nursing students is a factor affecting career maturity (r=-.510, p=.000) and career preparation behavior (r=-.353, p=.001). Therefore, It can be seen that it is necessary to develop an intervention program that can positively enhance the career maturity and career preparation behavior of nursing students through intervention for the moral anguish experienced by nursing students.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.44 no.6
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• pp.28-35
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• 2012

Water-in-oil emulsion (W/O) and oil-in-water emulsion (O/W) types biodegradable polymer emulsions prepared to PLA and PBS. The optimal mixing ratio of polymer : solvent : OA : TEA : water was found be 10 : 40 : 4 : 6 : 30(g) when preparing emulsions. Biodegradability was most retained after preparation of polymer emulsions. Particle size of PLA and PBS emulsions were 2-3 ${\mu}m$ and 3-4 ${\mu}m$, respectively. Molecular weight of PLA and PBS emulsions were 108,000 and 92,000, respectively. And molecular weight of PLA and PBS emulsions became slightly lower than those of pellets.

• Journal of Animal Science and Technology
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• v.60 no.8
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• pp.20.1-20.4
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• 2018

Platelet rich plasma (PRP) is popularly used in the horse industry to enhance regeneration of tissue injury that has limitation of blood supply. This study aimed to compare the methods for platelet rich plasma preparation since they has not been established yet. Blood was collected from six horses and platelets were concentrated by three different methods (2-step centrifugation, separated centrifugation and separated centrifugation using histopaque). Concentrated blood was analyzed using Advia hematology systems. In the result, separated centrifugation with histopaque showed the significantly lower number of red blood cells than other groups. The 2-step centrifugation showed the significantly higher number of white blood cells than other groups, while it contained the highest concentration of red blood cells among three groups. In the 2-step centrifugation, separated centrifugation and separated centrifugation with histopaque, platelets were concentrated 4.5, 5.3 and 5.6 times, respectively. And no significant difference of the platelet concentration between the three groups was found. This study demonstrated that separated centrifugation using histopaque was the best method for platelet rich plasma preparation because of the proper amount of platelets and the separation of red blood cells from platelet rich plasma.

• The Korean Journal of Pharmacology
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• v.10 no.1 s.15
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• pp.47-52
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• 1974

The isolated rabbit gall bladder strips were prepared according to the technique described by Amer and Becvar (1969). The strips were placed in a bath containing 100 ml of Locke-Ringer solution maintained at $38^{\circ}C$. Oxygen was continuously bubbled through the solution. The tension of the muscle strip was initially adjusted to 0.7g. The contractile response was measured isometrically by a force-displacement transducer connected to a polygraph. The effect of a number of autonomic drugs were studied for their interaction with caerulein (Prof. V. Erspamer, F.I. 6934 Caerulein, Farmitalia, Italia), a gastrin or CCK.PZ like peptide, on isolated rabbit gall bladder strips. In this preparation caerulein produced contractions of CCK-PZ type, but the relative potency on a weight basis was 40 times that of CCK-PZ. The response of caerulein was not modified by either cholinergic or alpha or beta adrenergic blockade. However, the response of caerulein and of barium on the strips were prevented by papaverine or aminophylline. Isoproterenol, papaverine or aminophylline alone relaxed the preparation whereas caerulein, CCK-PZ, acetylcholine, serotonin, histamine or barium chloride contracted the preparation. In summary, it is concluded that caerulein on the gall bladder strip seems to act independently of the autonomic nervous system and mediated via mechanisms apparently similar to those involved in the action of barium chloride.

• The Journal of Korean Medicine
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• v.30 no.4
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• pp.37-46
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• 2009

Objectives: The purpose of this study was to investigate the external application of paste preparation recorded in the Annals of the Chosun Dynasty (朝鮮王朝實錄) and to clarify the significance of modern use of plaster therapy. Methods: Of many paste preparations, records of All-applying ointment (萬應膏), One-great ointment (太一膏), Pain-relieving plaster (救苦膏), and Pus-promoting powder (促膿膏) were especially examined from the Annals of the Chosun Dynasty. Other medical records regarding how these preparations were passed onto later generations were also studied. Results: According to the records from the Annals of the Chosun Dynasty, paste preparations were mainly used to cure abscess and sore, partly to treat pain diseases. From other medical documents, it could be found that these preparations were continuously used in subsequent eras. Conclusions: From these records, the level and features of Chosun Dynasty medicine could be speculated. It is necessary to find and restore effective paste preparations in Traditional Korean Medicine to use for treating disease today. Therefore, records from the Annals of the Chosun Dynasty are important and meaningful materials.

• Preventive Nutrition and Food Science
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• v.4 no.1
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• pp.33-37
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• 1999

The antimutagenic effects of buchu kimchi and Chinese cabbage kimchi and theri cytotoxic effects against human cancer cell line were investigated in the Salmonella typhimurium system and MTT assay, respectively. Leek and Chinese cabbage were aslo evaluated in the same system. Buchu kimchi was fermented at 15 $^{\circ}C$ for 4 days . Buchu kimchi samples showed somewhat higher antimutagenic effects against aflatoxin B1(AFB1) than CHinese cabbage kimchi in Salmonella typhimurium TA100 strain. There was no difference onthe antimutagenic activity according to the length of fermentation . Leek exerted stronger antimutagenicity against AFB1 than Chinese cabbage in the Ames assay. In MTT assay, 6-day fermented buchu kimchin revealed the highest cytotoxicity against AGS human gastric adenocarcinoma cells in which 62% and 82% of the inhibition were observed wiht the addition of 100ug, 400ug/well, respectively. Buchu kimchi samples caused 60~70% inhibition on the proliferation of HT-29 at 400ug/well. Leek exhibited higher antiproliferative effect against both AGS cells and HT-29 cells than Chinese cabbage in MTT assay. From these results, it is considered that buchu kimchi has stronger antimutagenic and in vitro anticancer effects than Chinese cabbage kimchi and the high inhibition rate of buchu kimchi probably results from leek, the major ingredient of buchu kimchi .

• Clinics in Shoulder and Elbow
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• v.16 no.2
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• pp.153-162
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• 2013

The arthroscopic bony procedures during rotator cuff repair include acromioplasty, distal clavicle resection, footprint preparation and coracoplasty. The indication of each bony procedure is based on the theoretical reasons, and various types of surgical techniques are available. The purpose of this review article is to review the backgrounds of the indication and surgical techniques in the arthroscopic bony procedures during rotator cuff repair.

• Fashion & Textile Research Journal
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• v.24 no.3
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• pp.325-332
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• 2022

The purpose of this study is to provide basic data for career guidance to cosmetology students by examining the effects of job stress on career decisions and job preparation behaviors. Overall 214 parts were empirically analyzed as final samples. Collected data and statistical processing are based on SPSS ver. Analysis was performed using the 21.0 program. An analysis of the effect of job stress on career decision showed that employment anxiety (β= .206, p<.05), job anxiety(β=.824, p<.05), and anxiety in career search(β=.118, p<.05) showed a statistically significant effect(+). However, employment concerns(β=-.312, p<.001) in career determination and employment concerns(β=-.223, p<.01) in career search are statistically significant parts(-). An analysis of the effect of employment stress on employment preparation behavior found that employment anxiety(β=.364, p<.05) has a statistically significant effect(+) on information use preparation, It was found that the statistically significant part(-) affects employment anxiety(β=-.188, p<.01). The study found that the more anxious cosmetology students are about employment, the more they plan and explore career options, and make information-based preparations for employment. Through this research, we hope that there will be lively discussions among cosmetology students on career adaptability, life satisfaction, and employment anxiety.

• The Korea Journal of Herbology
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• v.36 no.1
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• pp.51-57
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• 2021

Objects : In this study, I aimed to determine the size and administration Method of pills preparation in Treatise on Cold Damage Diseases. Methods : I investigated the Treatise on Cold Damage Diseases, Newly Revised Materia Medica, Variorum of the Classic of Materia Medica, The Korean Herbal Pharmacopoeia, and recent studies. Results : There were four kinds of pills preparation. Each size of pill preparation was equal to the Odongja, Tanwhan, Yolk, and quarter of daily dosage of Decoction. One pill of Tanwhan was equivalent to 16 pills of Odongja, and one Yolk was equal to 40 pills of Odongja. The Diameter of Odongja was 6.5 mm, Tanwhan was 16.37897 mm, and Yolk was 22.11484 mm. Jeodangwhan was equal to one quarter daily dosage of Jeodangtang. Maximum size to swallow with water was Odongja and daily dosage, which was 16 pills, includes one square-inch-spoon of powder preparation. When we made the pill preparation, it is difficult to make 16 pills using one square-inch-spoon of powder. Therefore, Zhang Zhongjing made one dosage started from 10 pills to 20 pills to find out optimum dose. Due to the difficulty of making precise size of pills and considerable amount of daily dosage, there was no smaller size than Odongja. When the size of Pill preparation was larger than Odongja, it was ground or boiled slightly to make suspensions. Conclusions : These results suggest that the sizes of Pill preparation in the Treatise on Cold Damage Diseases were true compared with reality.