Journal of Pharmaceutical Investigation
- Volume 31 Issue 4
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- Pages.241-256
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- 2001
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- 2093-5552(pISSN)
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- 2093-6214(eISSN)
Evaluation of Alginate Microspheres Prepared by Emulsion and Spray Method for Oral Vaccine Delivery System
유화법과 분무법에 의해 제조된 경구백신용 알긴산 마이크로스피어의 평가
- Jiang, Ge (College of Pharmacy, Chungnam National University) ;
- Jee, Ung-Kil (College of Pharmacy, Chungnam National University) ;
- Maeng, Pil-Jae (Department of Microbiology, College of Natural Science, Chungam National University) ;
- Hwang, Sung-Joo (College of Pharmacy, Chungnam National University)
- Published : 2001.12.20
Abstract
Alginate microspheres, containing fluorescein isothiocyanate-bovine serum albumin (FITC-BSA) or green fluorescent protein (GFP) were prepared and used as a model drug to develop the oral vaccine delivery system. The alginate microspheres were coated with poly-L-lysine or chitosan. Two methods, w/o-emulsion and spray, were used to prepare alginate microspheres. To optimize preparation conditions, effects of several factors on the particle size and particle morphology of microsphere, and loading efficiency of model antigen were investigated. In both preparation methods, the particle size and the loading efficiency were enhanced when the concentration of sodium alginate increased. In the w/o-emulsion preparation method, as the concentration of Span 80 was increased from 0.5% to 2%, the particle size was decreased, but the loading efficiency was increased. The higher the emulsification speed was, the smaller the particle size and loading efficiency were. The concentration of calcium chloride did not show any effect on the particle size and loading efficiency. In the spray preparation method, the particle size was increased as the nozzle pressure
Keywords
- Alginate microsphere;
- FITC-BSA;
- GFP;
- Emulsion method;
- Spray method;
- Poly-L-lysine;
- Chitosan;
- Peyer's patch