• 대한본초학회지
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• 제27권5호
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• pp.65-75
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• 2012

Objectives : Cosmeceutical activities such as anti-oxidative and anti-aging effects of the Doinseunguitang and its composition, a traditional prescription, were evaluated. Methods : We performed MTT assay, melanin synthesis inhibition assay, DPPH free radical scavenging assay, SOD-like activity, xanthine oxidase inhibition assay, astringent activity assay, tyrosinase inhibition assay, elastase and collagenase inhibition assay. Results : The results were obtained as follows : DPPH free radical scavenging of water extract Doinseunguitang (DISG) and ethanol extract DISG was 60% and 50% at 1,000 ppm. Xanthine oxidase inhibition effect of ethanol extract showed more than 80% at 500 ppm. Tyrosinase inhibition and inhibition melanin synthesis activities were measured in 40% and 50% at 1,000 ppm and 100 ppm. Elastase and collagenase inhibition rate of ethanol extracts DISG 40% and 80% at 1,000 ppm. It was concluded that compositive ingredients (Persicae Seman, Glycyrrhizae Radix, Cinnamomi Ramulus, Rhei Rhizoma) influenced the most results. Conclusions : The results indicated that, ethanol extract which is superior in its anti-oxidative and anti-aging effects is useful to be applied in herbal cosmetic industry.

• 혜화의학회지
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• 제7권1호
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• pp.939-955
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• 1998

Achyranthis Bidentatae Radix(ABR) is important prescriptions that have been used in oriental medicine for stomatitis and wound healing. The study was done to evaluate the inhibitory effects of cytotoxicity, formation of superoxide on the macrophage and neutrophil, prostaglandins($PGE_2$), interleukins($IL-1{\beta}$), collagenase activity and synthesis of collagen and DNA. The results were obtained as follows: 1. ABR was not showed the proliferation difference of human fibroblast and monocyte in 0.01% and 0.001% concentrations to be experimented and in result, it was concluded that they have no cytotoxicity but showed cytotoxicity in 0.1% concentrations. 2. ABR inhibited the formation of superoxide to 48% at the concentration of 0.001% in the mouse monocyte. 3. ABR inhibited the formation of superoxide to 40% at 0.001%, 58% at 0.0001% as compared with control in the human monocyte. 4. ABR inhibited the formation of superoxide to 58% at 0.0001%, 40% at 0.001% in the human neutrophil. 5. ABR was not showed the proliferation difference of human monocyte in all concentrations to be experimented and in result, it was concluded that they inhibited the formation of prostaglandins($PGE_2$) in the human monocyte stimulated with E. coli. 6. ABR showed the all concentration of inhibiting the production of inter1eukins($IL-1{\beta}$) in the human monocyte stimulated with E. coli. 7. ABR didn't influence on collagen synthesis and total protein in fibroblasts. 8. ABR inhibited the collagenase activity to 84% at 0.1%, 69% at 0.2%, 76% at 0.5%, 91% at 0.001% respectively.

• 혜화의학회지
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• 제7권1호
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• pp.837-852
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• 1998

Ulmus parvifolia(UP) is important prescriptions that have been used in oriental medicine for stomatitis and wound healing. The study was done to evaluate the inhibitory effects of cytotoxicity, formation of superoxide on the macrophage and neutrophil, prostaglandins($PGE_2$), interleukins($IL-1{\beta}$), collagenase activity and synthesis of collagen and DNA. The results were obtained as follows: 1. UP was not showed the proliferation difference of human fibroblast and monocyte in all concentrations to be experimented and in result, it was concluded that they have no cytotoxicity. 2. UP inhibited the formation of superoxide to 22% at 0.01%, 52% at 0.001% in the mouse monocyte. 3. UP inhibited the formation of superoxide to 6% at the concentration of 0.001% as compared with control in the human monocyte. 4. UP was not showed the proliferation difference of human neutrophil in all concentrations to be experimented and in result, it was concluded that they inhibited the formation of superoxide. 5. UP was not showed the proliferation difference of human monocyte in all concentrations to be experimented and in result, it was concluded that they inhibited the formation of prostaglandins($PGE_2$) in the human monocyte stimulated with E. coli. 6. UP was showed the all concentration of inhibiting the production of interleukins($IL-1{\beta}$) to slight in the human monocyte stimulated with E. coli. 7. UP influence on collagen synthesis and total protein in fibroblasts to at the slight of 0.05%, specially to excellent to 0.2%. 8. UP inhibited the collagenase activity to 20% at 0.1%, 31% at 0.2%, 45% at 0.5%, 24% at 0.01% respectively.

• 생명과학회지
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• 제26권7호
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• pp.782-788
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• 2016

본 연구에서는 화장품의 천연소재로 사용 가능함을 알아보기 위해 추출부위가 각각 다른 총 11종의 자생식물의 항산화, 항주름에 관한 생리활성 효능을 스크리닝 하였다. DPPH scavenging assay 측정결과 100 μg/ml 농도에서 70% 넘는 활성을 나타낸 시료는 순비기나무, 송이고랭이, 방울고랭이, 큰고랭이, 자귀풀이었다. Hydrogen peroxide scavenging assay 측정결과 1,000 μg/ml 농도에서 물꼬챙이골을 제외한 10종이 90%넘는 활성을 나타내었다. Xanthine oxidase scavenging assay 측정결과 1,000 μg/ml 농도에서 순비기나무(Leaf; 86.3%)가 가장 높은 활성을 나타냈다. 4가지 항산화 실험에서 모두 효능이 우수하게 나온 시료는 순비기나무, 송이고랭이, 방울고랭이, 큰고랭이, 자귀풀이며 그중 collagenase 저해활성 측정결과 방울고랭이가 가장 높은 저해 활성을 나타내어 항산화 및 항주름에 효능이 있는 시료로 나타났다. 이러한 결과를 바탕으로 활성이 높은 자생식물은 화장품에 사용되는 천연소재로 이용이 가능한 좋은 수종으로 판단된다.

• 한국균학회지
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• 제47권2호
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• pp.153-163
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• 2019

본 연구는 갯버들(Salix gracilistyla)의 화장품 소재로의 활용가능성을 판단하기 위해, 기존 일반적인 방법이 아닌 균사배양 방법을 통해 가능성을 확인하였고, 구체적으로는 대표적인 약용버섯인 불로초(Ganoderma lucidum) 균사의 배양을 통하여 그 효능을 확인하였다. 총 폴리페놀 및 플라보노이드 함량과 DPPH 라디칼소거능 분석결과에서는, 갯버들 추출물(SGE)이 불로초 균사배양 갯버들 추출물(GLSGE) 보다 높게 평가되었으나, 콜라게나제 활성 억제 평가에서는 불로초 균사배양 갯버들 추출물(GLSGE)이 갯버들 추출물(SGE)과 비교하여 상대적으로 낮게 평가되었다. 그럼에도 불구하고, 불로초 균사배양 갯버들 추출물(GLSGE)은 갯버들 추출물(SGE)을 처리한 경우보다, HDFn 세포의 생존율을 높은 수준으로 증가시켰으며, HDFn 세포의 제1형 프로콜라겐의 생합성 또한 매우 높은 수준으로 증가시키는 것이 확인되었다. 결과적으로 앞선 실험을 통해, 불로초 균사배양 갯버들 추출물이 항노화 활성을 가지는 기능성 화장품 소재로 활용 가능성이 있다고 사료되며, 기능성 소재의 효과를 더욱 증진시키기 위한 방안으로 불로초 균사의 배양이 효과적이라는 것을 의미한다.

• 약학회지
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• 제51권5호
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• pp.343-349
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• 2007

To investigate the effect of ethanol extract of Saxifraga stolonifera MEERBURGH on skin care, we measured anti-oxidant and anti-aging activity. S. stolonifera ethanol extract itself had anti-oxidant activity in a dose-dependent manner in DPPH radical scavenging. Silica dose-dependently increased the intracellular ROS generation in RAW 264.7 cells. S. stolonifera ethanol extract inhibited silica-induced intracellular superoxide anion generation, $H_2O_2$ and hydroperoxide generation in RAW 264.7 cells. S. stolonifera ethanol extract significantly inhibited both hyaluronidase and elastase activity, also significantly inhibited MMP-1(collagenase) activity as well. In NIH 3T3 fibroblast cells, S. stolonifera ethanol extract significantly increased collagen-like polymer synthesis, which suggesting the S. stolonifera ethanol extract might be used as hydration and anti-wrinkle agents. From the above results, it is suggested that the main ingredients of S. stolonifera ethanol extract play an important role in anti-oxidant and anti-aging activity.

• KSBB Journal
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• 제15권6호
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• pp.635-643
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• 2000

To compare the antioxidative activities of fish skin and bovine skin gelatin hydrolysate, gelatin hydrolysates from Alaska pollack and bovine skin were prepared by various enzymatic hydrolysis methods (1st step, Alcalase; 2nd step, pronase E; 3rd step, collagenase) using a continuous three-step membrane reactor. The molecular weight distributions of the 1st, 2nd and 3rd step hydrolysates were 7∼10 kDa, 2∼5 kDa and 0.7∼0.9 kDa, respectively. The antioxidative activity of fish skin gelatin hydrolysate was stronger than that of bovine skin gelatin hydrolysate, and in particular, both of 2nd step hydrolysates showed more antioxidative activity than hydrolysates of any other step. The optimum antioxidative activity concentration of the 2nd step hydeolysates of fish and boving skin were 1% (w/w) in a linoleic acid water-alcohol emulsion. In cultured cells exposed to t-butyl hydroperoxide (t-BHP), the 2nd step hydrolysate of fish skin gelatin delayed cell death most. These results suggest that the antioxidative activity of fish skin gelatin hydrolysate is higher than that of bovine skin gelatin hydrolysate because of their different amino acid contents.

• 생명과학회지
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• 제10권2호
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• pp.218-227
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• 2000

Bone morphogenetic protein 1 (BMP-1) is part of a complex capable of inducing ectopic bone formation in mammals. Studies on TGF-β1 processing and Drosophila dorsal-ventral patterning have focused attention on BMP-1 as important in mediating the biological activity of this bone inducing complex. Herein, the bacterial expression, refolding, purification, and initial characterization of the BMP-1 proteolytic domain (BPD) are described. A semi-quantitative fluorescence-based thin layer chromatography assay was developed to assist in rapidly screening for optimal renaturation conditions. According to a preliminary screen for optimal conditions for the refolding of BPD , a detectable proteolytic activity against a high turnover substrate for astacin, a homologous protease from crayfish was observed. The conditions identified have allowed the expression of sufficient amounts of BPD for the characterization of the protein. Its proteolytic activity exhibits the same cleavage specificity as astacin against seven substrates that were previously synthesized for studying astacin. Furthermore, this activity is inhibited by the metal chelator 1,10-phenanthroline but not by its analogue 1,7-phenanthroline. The collagenase inhibitor Pro-Leu-Gly hydroxamate was found to inhibit both astacin and BPD activity. The results presented in this paper argue that BMP-1 does in fact possess an intrinsic proteolytic activity.

• 한국수산과학회지
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• 제56권6호
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• pp.833-840
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• 2023

In this study, the antioxidant and anti-skin aging properties of the Korean marine algae Ishige foliacea were investigated. Solvent extracts from I. foliacea were prepared with 70% ethanol, 80% methanol, and water. The extraction yields of various solvent extracts ranged from 9.55% to 35.12%. In terms of antioxidant activity, the ethanol extract showed the highest ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) radical scavenging activity, nitrite oxide scavenging activity, reducing power, and FRAP (ferric reducing antioxidant power). Regarding anti-skin aging activity, evaluation of the skin whitening and anti-wrinkle activities revealed that the methanol, water, and ethanol extracts possessed the highest tyrosinase (IC₅₀=0.98 mg/mL), elastase (IC₅₀=0.15 mg/mL), and collagenase (IC₅₀=0.06 mg/mL) inhibitory activities, respectively. These results suggest that I. foliacea holds potential as an antioxidant and anti-skin aging substance in food and cosmetic materials.

• 대한화장품학회지
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• 제36권4호
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• pp.281-288
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• 2010

본 연구는 에탄올 농도별(0, 30, 50, 70, 100 %)로 추출한 사매추출물의 항산화 효과를 Oxygen Radical Absorbance Capacity(ORAC)로 평가한 결과 30 % 에탄올 추출물에서 가장 우수하였다. 따라서 30 % 에탄올 추출물의 주름 완화에 관련한 효능 및 효과를 확인하기 위하여 마우스 유래 섬유아세포에 대한 세포 독성, MMP-8 collagenase 억제 활성 검사를 실시하였다. 그 결과 30 % 에탄올 사매추출물을 섬유아세포에 처리한 결과 세포 독성이 없었고, 20 %의 MMP-8 collagenase 억제 활성을 보였다. 사매 추출물 1 %가 함유된 Di-Wrinkle Free Cream (DWFC)으로부터 35 ~ 50세의 21명 여성을 대상으로 하루에 2번 도포하여 8주간 인체 안전성과 주름개선 임상평가를 수행하였다. 주름 평가 방법은 3차원 입체 측정기(PRIMOS)를 이용하여 평가를 하였다. DWFC에 대한 안전성 평가는 피험자 방문 시 마다 이상반응, 부종, 인설, 가려움, 자통, 작열감, 뻣뻣함, 따끔거림이나 다른 이상반응이 발생하는지 육안평가와 문진으로 평가하였다. PRIMOS(GFM, Germany)를 이용하여 눈 주변의 주름을 분석한 결과 시험제품 사용 8주 후 통계적으로 유의하게 주름이 13 % 감소하였으며, 시험기간 동안 특별한 피부 이상반응은 관찰되지 않았다. 이와 같은 결과로 보아 사매추출물은 인체에 부작용이 없는 주름개선에 효과 있는 화장품으로 개발이 가능할 것으로 사료된다.