• 한국작물학회지
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• 제39권3호
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• pp.232-236
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• 1994

보리 4품종, 밀 4품종, 호밀 3품종, 옥수수 1품종을 가지고 파종 심도를 달리 하였을 때 이들 작물들의 초엽장과 식물체장을 조사하였고 또 이들 간의 상관을 조사한 결과를 요약하면 다음과 같다. 1. 보리, 밀, 호밀, 옥수수가 발아할 때 생기는 관 아래의 절간을 중경 또는 지하경 이라고 호칭하고 있는데 지하경으로 부르는 것이 옳을 것 같으며 보리, 밀, 호밀은 초엽이 종자로부터 지하경과 함께 나오고 옥수수는 지하경 정단의 관에서 나오는 것을 확실하게 해두는 것이 좋을 것 같다. 2. 보리의 초엽의 길이는 파종 심도가 깊어질수록 현저하게 길어지고 식물체장도 같은 경향이 있는데 보리의 2cm파종은 6cm, 10cm깊이 파종 보다 현저하게 짧게 나타났다. 품종간에는 차이를 볼 수 없었다. 3. 밀의 초엽 길이도 마찬가지로 파종 심도에 따라 현저하게 길어졌으며 2cm 파종은 6cm, 10cm 파종보다 현저하게 짧게 나타났으나 보리보다는 못하였다. 이것도 품종간 차이는 별로 나타나지 않았다. 4. 호밀과 옥수수도 파종 심도가 깊어질수록 초엽이 길어졌으며 그 변이가 보리와 밀보다 크게 나타났다. 호밀과 옥수수에서 변이가 컸다. 5. 각 작물과 품종들에 있어서 초엽과 식물체장 간에 유의성이 있는 상관을 보였고 칠보 호밀은 고도의 유의성을 나타내었다.

• Journal of Plant Biology
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• 제34권4호
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• pp.317-323
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• 1991

Partial recovery in auxin transport capacity from inhibition by N-naphthylphthalamic acid (NPA) was observed when corn coleoptile segments were subjected to a prolonged NPA treatment. Kinetic data indicated that the recovery time is a function of the concentration of NPA applied. Desensitization to NPA was also seen in tissue slices where NPA increased net uptake of auxin, indicating that the apparant adaptation in the auxin transport system did not results possibly from auxin accumulated during transport inhibition. Studies on in vitro binding of NPA to membrane vesicles isolated from the coleoptile indicated that preincubation of the tissue with NPA resulted in the reduced binding activity. Scatchard analysis of the data indicated that this was due to decreases in the number of NPA binding sites. The possibility of causal relationship of modified NPA receptors to the sensory adaptation in auxin transport observed in coleoptile segments will be discussed.

• 한국작물학회지
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• 제45권1호
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• pp.50-54
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• 2000

The elongation of mesocotyl and coleoptile plays important roles in the seedling emergence and stand establishment of dry direct-seeded rice. Experiments were carried out to elucidate the effects of seed-presoaking treatments of GA$_3$ and some inorganic salts on the mesocotyl, and coleoptile elongation of rice. Seed-soaked GA$_3$ promoted the elongation of mesocotyl, but little effect on the coleoptile elongation. The stimulation effects of GA$_3$ were found to be enhanced by addition of CaCl$_2$ However, the sole treatment of CaCl$_2$ showed no stimulating effect on the mesocotyl and coleoptile elongation. Mesocotyl elongation was most prominent in the combined treatments of 50ppm GA$_3$ with 100 mM CaCl$_2$. The synergistic effects of GA$_3$ and CaCl$_2$ on mesocotyl elongation varied with varietal groups. The stimulating effects of GA$_3$ were enhanced significantly by the addition of CaCl$_2$ in japonica varieties, Dongjinbyeo, Ilpumbyeo and Milyang 95, and tall indica variety, Labelle, but not in semidwarf Tongil type varieties, Tongilbyeo, Milyang 23, and Nampungbyeo, and semi-dwarf indica, Short Labelle. The promoting effects of GA$_3$ on the mesocotyl elongation were decreased in proportion to the lowered osmotic potential by PEG 6000 on the contrary to CaCl$_2$ This implies that the synergistic effects of CaCl$_2$ with GA$_3$ on mesocotyl elongation was not caused by osmotic potential lowered by CaCl$_2$ addition but by the salt itself. Salts such as Ca(NO$_3$)$_2$, MgCl$_2$ BaCl$_2$, NaCl, KCl and KNO$_3$ showed the synergistic effects with GA$_3$ on mesocotyl elongation as well. The degree of synergistic effects showed no differences among salts tested, implying that there is no specificity of ions constituting the salts.

• 한국작물학회지
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• 제44권1호
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• pp.11-19
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• 1999

This experiment was conducted with nine wheat geno-types to choose the wheat which has excellent early vigour. 'Vigour 18' and 'ZL 59A' are excellent in the long coleoptile genotype, while 'Amery' and 'Janz' are excellent in the short coleoptile genotype. Responding to the growth stage and nitrogen level, Vigour 18 is predominant in the long coleoptile genogype, while Janz in the short coleoptile genotype. Responding to sowing density and nitrogen level, the higher the sowing density was, the shorter the leaf area of Vigour 18 and Janz. Also the leaf area turned out to larger in the plot fertilized with high nitrogen than in the plot fertilized with low nitrogen. This is true of leaf weight and root weight. Concerning specific leaf area (SLA) and leaf area ratio (LAR), the higher the sowing density was, the SLA tended to grow larger, while the SLA grew larger in the plot fertilized with low nitrogen, as were found in Vigour 18 and Janz. The roots of long coleoptile genotype, Vigour 18, turned out to grow longest on the plot sown with 3 seeds. While the roots of short coleoptile genotype, Janz, grew longest on the plot sown with 2 seeds. The relative growth rate (RGR) was the same at low N rates and high N rates. The RGR was 0.071 and 0.072 g $g^{-1}d^{-1}$ at low N rates and high N rates. The partitioning of RGR into net assimilation rate (NAR) and LAR showed that the average LAR at low N rates was similar to the LAR at high N rates. Variation within each cultivar in the LAR and NAR was small relative to the difference between them at low N rates and high N rates. Above ground mass was 8.2 mg greater at high N rates than low N rates, whereas leaf area was 0.05 $\textrm{m}^2$$kg^{-l}$ greater at high N rates than low N rates. The NAR was similar at low N rates and high N rates, whereas LAR was greater at high N rates (0.05 $\textrm{m}^2$$kg^{-l}$); variation in SLA was responsible for the variation in NAR and LAR both at low N rates and high N rates. NAR was more closely associated with the reciprocal of SLA.

• 한국자원식물학회지
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• 제31권5호
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• pp.522-530
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• 2018

Brachypodium distachyon has been developed as a monocot model plant for temperate grasses and bioenergy crops. Although B. distachyon research is moving forward rapidly, the study of photoresponses has not been explored. To extend our knowledge of responses to light in monocots, we performed photoresponse analysis of B. distachyon using two inbred lines, Bd21 and Bd21-3. In this study, we first compared growing phenotypes between the two lines and investigated coleoptile and primary leaf growths under dark, far-red, red, and white light conditions. The results showed that the growth of the two lines were similar until tillering stage, but other developmental stages from heading to senescence were much delayed in Bd21-3, which resulted in increased height and tiller numbers. Under different light conditions, primary leaf lengths were kept increasing during the growth period, whereas the coleoptile extension was inhibited 4 to 7 days after growth depending on the light conditions applied. These results suggest that the responses to light in B. distachyon can be examined by measuring coleoptile lengths approximately 7 days after seedling growth. Moreover, we selected light-responsive genes known in Arabidopsis thaliana, such as chlorophyll A/B binding protein (CAB), light-harvesting chlorophyll binding protein (Lhcb) and chalcone synthase (CHS), and confirmed their light-induced gene expression in B. distachyon. Therefore, the present study suggests that the inhibition of coleoptile growth can be used as the parameter to analyze photoresponses in the monocot model plant, and also provide the reference genes whose expression is induced by far-red and red light treatment.

• Journal of Plant Biology
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• 제39권2호
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• pp.99-105
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• 1996

Immunocytochemistry utilizes the specificity of the antigen-antibody reaction to localize specific antigens in cells or cellular organelles. Here we report the use of monoclonal antibodies, in conjunction with gold-labeled second antibodies to study the ultrastructural localization and tissue distribution of the Mr 98, 000 anionic peroxidase and other wall antigens. The antibody specific for this wall peroxidase, mWP3, labeled mainly the cell wall area. At the tissue level, the Mr 98, 000 peroxidase is located predominantly in the leaf mesophyll, internal coleoptile and sieve elements, but not in the root, as assayed with these procedures. The coleoptile walls were less heavily stained than the walls of leaf mesophyll cells. At the subcellular level, it is localized mainly in intercellular regions of the cell walls. A similar staining pattern was revealed by mWP19, one of anti-$\beta$ glucosidase antibody, though it looked less heavily stained than one with mWP3. In order to serve as a control wall staining using IgM monoclonal antibodies, mWP18 was used. Most of the label is localized over wall regions of cells of the young leaf mesophyll and coleoptile.

• Journal of Plant Biology
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• 제33권4호
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• pp.315-320
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• 1990

We investigated the interaction of auxin and Ca2+ on coleoptile segment elongation in seedlings of Zea mays L. Seedlings imbibed and raised either in the presence of 10 mM CaCl2 (HC), or in the absence of Ca2+ (LC) were used. Exposure to 10-5M auxin of coleoptiles from either HC or LC seedlings resulted in strong promotion of elongation. However, longer latent period (90 min) of the auxin effect was observed in HC than in LC seedlings (20 min). The length of latent period observed in HC coleoptiles was proportional to the concentration of CaCl2. The latent period of auxin effect observed in HC seedlings was abolished by pretreatment of the coleoptiles with TMB-8 which inhibits IP3-induced Ca2+ release from the tonoplasts. In segments of LC seedlings, the promotive effect of IAA (10-5M) was abolished by treatment with 5 mM calcium but was reversible upon treatment of the segments with 5 mM EGTA. These results suggest that the effect of auxin on coleoptile elongation is closely related to intracellular Ca2+ level.

• 아시안잔디학회지
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• 제3권2호
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• pp.73-76
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• 1989

To establish a lawn Zoysia japonica Steud with seeds a win of experiments were conducted for the investigation of seedling root system. The results m summarized m follows; Zoysia japonica and maize elongated mesocotyle in germinating stage. but rye and barley did not. The mesocotyle of Z. japonica seed pushed the elongating coleoptile up throngh the soil, hence could emerge from more deeply planted. The crown roots of Z japonica originated from the coleoptile node. The crown roots of barley originated from the first foliage led node.

• Journal of Plant Biology
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• 제34권4호
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• pp.297-302
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• 1991

The effects of polyamines on auxin polar transport were studied in corn coleoptile segments. Among putresine, spermidine and spermine tested in labelled auxin transport, spermidine inhibited auxin polar transport most strongly. Its inhibitory effect appeared after 1 h of transport period. Spermidine inhibited labelled auxin and 14C-benzoic acid accumulation into the tissue in the various pH range tested (pH 4.0-8.0). These results suggest that the inhibition of auxin transport may not be due to decrease in pH by spermine the effect of decreased pH in the extracellular space.

• Journal of Plant Biology
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• 제34권4호
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• pp.325-330
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• 1991

The ability of several auxin analogs to induce ethylene production was tested in the corn coleoptile. The synthetic auxins 1-naphthaleneacetic acid (1-NAA) and 2, 4-dichlorophenoxyacetic acid (2, 4-D) had strong stimulatory effects on ethylene induction surpassing that of IAA. Both 2-naphthalaneacetic acid (2-NAA) and 2, 6-dichlorophenoxy acetic acid (2, 6-D), structural analogs of these auxins, respectively, were found to be inactive. Treatment with NPA, a strong inhibitor of polar auxin transport, led to drastic increase in IAA-induced ethylene production while it has bo effect on ethylene production induced by 1-NAA. A positive correlative existed between intracellular auxin level and ethylene production.