• Applied Biological Chemistry
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• v.26 no.1
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• pp.58-64
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• 1983

The fatty acid composition of phospholipid from the leaves of rice plants grown at $28^{\circ}C$ and harvested at the 3-4 leaf stage was determined for 8 cultivars. Change among cultivars observed in the composition has been found to be correlative to the chilling susceptivity of the plants at the given leaf-stage. The chilling-resistant cultivars contain phospholipid with higher degree of unsaturation and larger relative proportion of unsaturated fatty acids than the chilling-sensitive ones. The indices for unsaturation of phospholipid from a cultic-ar are well in accordance with its resistance to cold damage. clearly demonstrating that the fluidity of biomembrane which is generally regarded as the prerequisite for a cell to maintain its membrane-related physiological activity at a low temperature is exclusively controlled by the fatty acid composition of phospholipid. Also identified were the components of phospholipid, which are phosphatidyl serine and phosphatidyl coline as major components and phosphatidyl inositol as minor component plus 3 phospholipids in trace proportion, from every cultivar at the early growth-stage.

• Journal of Periodontal and Implant Science
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• v.35 no.3
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• pp.577-590
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• 2005

The purpose of this study was to evaluate the effects of toothpaste containing hydroxyapatite for patients who complained dental hypersensitivity. Before baseline of application of toothpaste with hydroxyapatite, tooth brushing instruction was done respectively and the several indices were measured at baseline, 2, 4, 8 weeks. Clinical indices were estimated, and responses to cold, compressive air, tactile stimulus were evaluated with verbal rating score. Relief effects and visual analogue scale were also evaluated. The results of this study were as follows 1. The occurrence rate of hypersensitivity in upper jaw was higher than that of lower jaws, and molar area showed more hypersensitivity than premolar and incisor area. Buccal site was hypersensitive followed by interproximal and lingual site. 2. Plaque index, gingival index and probing depth reduction were gradually improved after Tooth Brushing Instruction and using toothpaste. 3. Subjects showed most sensitive response to cold stimuli than compressive air and tactile stimuli. 4. The relief effect was increased during using tooth paste and complete relief was increased especially at 8 weeks. 5. Visual analogue scale was increased. In conclusion, it was confirmed that toothpaste containing microcrystalline hydroxyapatite have the relief effect of tooth hypersensitivity. During 8 weeks, stimulus responses were decreased and hypersensitivity relief effect was increased.

• The Korean Journal of Pain
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• v.25 no.2
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• pp.94-98
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• 2012

Background: An intravenous infusion of lidocaine has been used on numerous occasions to produce analgesia in neuropathic pain. In the cases of failed back surgery syndrom, the pain generated as result of abnormal impulse from the dorsal root ganglion and spinal cord, for instance as a result of nerve injury may be particularly sensitive to lidocaine. The aim of the present study was to identify the effects of IV lidocaine on neuropathic pain items of FBSS. Methods: The study was a randomized, prospective, double-blinded, crossover study involving eighteen patients with failed back surgery syndrome. The treatments were: 0.9% normal saline, lidocaine 1 mg/kg in 500 ml normal saline, and lidocaine 5 mg/kg in 500 ml normal saline over 60 minutes. The patients underwent infusions on three different appointments, at least two weeks apart. Thus all patients received all 3 treatments. Pain measurement was taken by visual analogue scale (VAS), and neuropathic pain questionnaire. Results: Both lidocaine (1 mg/kg, 5 mg/kg) and placebo significantly reduced the intense, sharp, hot, dull, cold, sensitivity, itchy, unpleasant, deep and superficial of pain. The amount of change was not significantly different among either of the lidocaine and placebo, or among the lidocaine treatments themselves, for any of the pain responses, except sharp, dull, cold, unpleasant, and deep pain. And VAS was decreased during infusion in all 3 group and there were no difference among groups. Conclusions: This study shows that 1 mg/kg, or 5 mg/kg of IV lidocaine, and palcebo was effective in patients with neuropathic pain attributable to FBSS, but effect of licoaine did not differ from placebo saline.

• Microbiology and Biotechnology Letters
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• v.13 no.1
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• pp.41-49
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• 1985

Bacillus sphaericus was mutagenized with UV light irradiation and dimethyl sulfate. Thirty-five conditional lethal temperature-sensitive(ts) mutants were isolated at the nonpermissive temperature of $42^{\circ}C$ and classified into three groups by their growth characteristics on the nutrient broth, peptone glucose yeast extract agar and mineral salts agar. First was the lethal ts group, 24 mutants, which did not grow at the nonpermissive temperature, the second, 9 mutants, was the less growth is group whose growth was restricted to one-half, and the third, 2 mutants, was the cold lethal ts group whose growth was restricted at the permissive temperature($25^{\circ}C$and $30^{\circ}C$)

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.2
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• pp.571-577
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• 2007

The purpose of this study was to investigate the relationship between the pain and the weather. One hundred ninety three patients treated for pain were recruited for this study. All subjects completed a 8-item weather and pain questionnaire to find out whether the pain are related to change in the weather, Of all subjects, 126 patients(65.3%) believed that change in the weather affected their pain. Of theses 126 patients, 85 patients(67.5%) reported that their pain was affected before weather changes, 44 patients(34.9%) stated that their pain was affected during weather changes. Damp/Rainy(n=91, 72.2%) and cold(n=60, 47.6%) conditions were mostly considered to have influence on pain. Of all subjects, 125 patients(64.8%) believed that change in the weather affected their mood. In the female group, a tendency to have pain or mood sensitivity to change in the weather was significantly higher than the male group(p=0.006, p=0.003) In the non-religious group, a tendency to have pain or mood sensitivity to change in the weather was significantly higher than the religious group(p=0.006, p=0.004). Of the pain sensitive group to change in the weather, 97 patients(77.0%) reported that they also have mood sensitivity to change in the weather(p=0.000). In the pain or mood sensitive group to change in the weather, their pain intensity(VAS) was significantly higher than the other group(p=0.000, p=0.021). The results of this survey give support to the idea that most patients with pain believe that weather has an important impact on their pain. Further investigations are needed to identify the mechanisms involved in the effects of weather changes on pain.

• Korean Journal of Food Science and Technology
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• v.28 no.1
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• pp.179-183
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• 1996

The objective of this study was to investigate the difference of oxidative stability between DHA (docosahexaenoic acid) added and not added commercial mayonnaise, and then to find out stable condition for maintaining the quality of DHA added mayonnaise. During the storage at room temperature $(25^{\circ}C{\pm}1^{\circ}C)$, it showed that DHA added commercial mayonnaise was more sensitive to oxidation than DHA not added commercial mayonnaise. And sensory quality of DHA added commercial mayonnaise decreased drastically after 5 months from the production date of mayonnaise. During the storage at low temperature $(4^{\circ}C{\pm}1^{\circ}C)$, DHA added mayonnaise was also more sensitive to oxidation than DHA not added mayonnaise, but it showed no apparent and sensory problem with both samples during 8 months of cold storage test periods. In considering the distribution condition of mayonnaise in the country, it was suggested that shelf-life of the DHA added commercial mayonnaise should be shortened 2 or 3 months than that of the DHA not added commercial mayonnaise.

• Journal of Veterinary Clinics
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• v.21 no.4
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• pp.329-335
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• 2004

Dog spermatozoa were recovered from the caudae epididymides of 23 domestic dogs which were 11 pure breed and 12 mix-breed dogs ranging in age from 0.6 to 3 years. The experimental designs were as follows: 1) the effect of chilling to 0. 10 or 37$^{\circ}C$. 2) the kinetics of chilling injury at 0 or 4$^{\circ}C$, and 3) the effect of sugars at $0^{\circ}C$. Viable spermatozoa were recovered by percoll gradient separation and adjusted to 5${\times}$10$^{7}$ spermatozoa/ml. In experiment 1, spermatozoa were diluted with 0.33 M glucose supplemented with 3% BSA (G-BSA) at 1:2 dilution. Spermatozoa were loaded into straws and exposed at 0, 10 or 37$^{\circ}C$ for 30 min. In experiment 2, spermatozoa were prepared as the experiment 1 and exposed for 0.5, 5, 15, or 30 min at 0 or 4$^{\circ}C$. In experiment 3, spermatozoa were diluted with different sugars (0.33 M galactose, glucose, fructose, mannitol, lactose, sucrose, raffinose) and cooled to $0^{\circ}C$ for 30 min. Sperm membrane integrity, motility and acrosome integrity were assayed after rewarming at 37$^{\circ}C$ for 5 min. Sperm motility and membrane integrity abruptly decreased with decreasing temperature but acrosome integrity gradually decreased (P<0.05). Sperm motility was more sensitive to cold shock than membrane integrity and acrosome integrity. Spermatozoa cooled to $0^{\circ}C$ were more damaged than those at 4$^{\circ}C$. Sperm motility was not different among exposed times at both. 0 and 4$^{\circ}C$. However, membrane integrity of spermatozoa exposed for 30 min at both 0 and 4$^{\circ}C$ was significantly lower (P<0.05). Spermatozoa diluted in 0.33 M fructose or galactose showed lower motility and higher morphological abnormality with coiled tail at $0^{\circ}C$. These sperm characteristics were strongly related. These results indicate that dog epididymal spermatozoa are relatively sensitive to rapid cooling and higher morphological abnormality at $0^{\circ}C$ was shown in spermatozoa diluted in fructose and galactose.

• Korean Journal of Ichthyology
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• v.22 no.4
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• pp.230-237
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• 2010

Histological observation on the seasonal variation of mucus cells of the mud loach Misgurnus mizolepis inhabiting a natural stream was carried out on three skin regions (dorsal, lateral and occiput) from March 2008 to February 2009. Our results showed no differences in general morphology by season, but the mucus cells of the epidermis showed significant seasonal change in their size and number as the water temperature changed. The ratio of surface area of the mucus cell layer and mucus cells, and the number of mucus cells in surface area of the epidermis were the greatest in the cold winter and the least in the hot summer in all regions of the epidermis. In particular, the occiput seemed to be a very sensitive region in response to environmental change, showing wide fluctuations in the size of mucus cells throughout the year and a great change in between seasons, especially from late autumn to early winter when the temperature decreased. As the temperature became colder, a small and spherical-shaped mucus cell was transformed into a large and elongated columnar form with a lot of secreted mucus material in a superficial layer of the epidermis. From our results, we can safely surmise that cold temperature is an important environmental factor having a close relationship with the modification of mucus cells of M. mizolepis in winter.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.16 no.4
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• pp.206-211
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• 2011

Low water temperature during the summer associated with the occurrence of cold water zone off Haeundae was studied using the data from CTD observations and a monitoring buoy deployed in Suyeong Bay. Shortterm variability of current was dominant and was not related to the wind. The NE-SW components of wind parallel to the coast contained more than 96% of total variance and could account for major fluctuations of water temperature. Upwelling-response of water temperature was very sensitive so that the temperature began to decrease immediately after the onset of the southwesterly wind. In particular, there were three cases in which SW winds for only two days caused considerable temperature drops. In 2009, four upwelling events shorter than 5 days took place while seven events with periods of 2~18 days were recorded in 2010. During a very intense upwelling for seven days in mid-August 2010, temperature decreased by more than $10^{\circ}C$ in spite of the variable winds. Temperature variability at Gampo, Ulsan, Gijang and Haeundae had similar patterns. CTD observation and satellite imagery showed that the upwelling zone could be extended to the Haeundae-Busan area. According to the wavelet analysis, coherent periods were 2~8 days during the frequent upwelling/downwelling events.

• International Journal of Highway Engineering
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• v.11 no.1
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• pp.1-12
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• 2009

A new mix design procedure for cold in-place recycling using foamed asphalt (CIR-foam) has been developed for Iowa Department of Transportation. Some strengths and weaknesses of the new mix design parameters were considered and modified to improve the laboratory test procedure. Based on the critical mixture parameters identified, a new mix design procedure was developed and validated to establish the properties of the CIR-foam mixtures. As part of the validation effort to evaluate a new CIR-foam mix design procedure, dynamic moduli of CIR-foam mixtures made of seven different reclaimed asphalt pavement (RAP) materials collected throughout the state of Iowa were measured and their master curves were constructed. The main objectives of this study are to provide: 1) standardized testing procedure for measuring the dynamic modulus of CIR-foam mixtures using new simple performance testing (SPT) equipment; 2) analysis procedure for constructing the master curves for a wide range of RAP materials; and 3) impacts of RAP material characteristics on the dynamic modulus. Dynamic moduli were measured at three different temperatures and six different loading frequencies and they were consistent among different RAP sources. Master curves were then constructed for the CIR-foam mixtures using seven different RAP materials. Based upon the observation of the constructed master curves, dynamic moduli of CIR-foam mixtures were less sensitive to the loading frequencies than HMA mixtures. It can be concluded that at the low temperature, the dynamic modulus is affected by the amount of fines in the RAP materials whereas, at the high temperature, the dynamic modulus is influenced by the residual binder characteristics.