• Journal of Microbiology and Biotechnology
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• v.29 no.6
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• pp.952-961
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• 2019

Chlorella spp. are green algae that are found across wide-ranging habitats from deserts to arctic regions, with various strains having adapted to survive under diverse environmental conditions. In this study, two novel Chlorella strains (ABC-002, ABC-008) were isolated from a freshwater lake in South Korea during the winter season and examined for possible use in the biofuel production process. The comparison of ABC-002 and ABC-008 strains with Chlorella vulgaris UTEX265 under two different temperatures ($10^{\circ}C$, $25^{\circ}C$) revealed their cold-tolerant phenotypes as well as high biomass yields. The maximum quantum yields of UTEX25, ABC-002, and ABC-008 at $10^{\circ}C$ were 0.5594, 0.6747, and 0.7150, respectively, providing evidence of the relatively higher cold-resistance capabilities of these two strains. Furthermore, both the biomass yields and lipid content of the two novel strains were found to be higher than those of UTEX265; the overall lipid productivities of ABC-002 and ABC-008 were 1.7 ~ 2.8 fold and 1.6 ~ 4.2 fold higher compared to that of UTEX265, respectively. Thus, the high biomass and lipid productivity over a wide range of temperatures indicate that C. vulgaris ABC-002 and ABC-008 are promising candidates for applications in biofuel productions via outdoor biomass cultivation.

• Korean Journal of Agricultural Science
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• v.47 no.4
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• pp.829-844
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• 2020

A vacuum metering device for garlic seeds was developed, and its metering performance was tested according to the design factors. Three design factors were as follows: suction surface diameter (Edge), suction surface curvature (Type), and guide height (Guide). The suction surface curvature represents the distance between the center of the grooved suction plate and the bottom of suction plate. The metering rate and multiple metering rate were analyzed as the metering performance of the developed device with two varieties of garlic seeds: Namhae (warm region-adapted garlic) and Uiseong (cold region-adapted garlic). The best metering performance for the Namhae seeds were found with the following conditions: An Edge, Guide and Type of 40, 4, and 35 mm, respectively. In the case of the Uiseong seeds, the best conditions were as follows: An Edge, Guide and Type of 35, 4, and 30 mm, respectively. The guide height was found to be the most influential design factor on the metering performance of the metering rate and multiple rate for both Namhae seeds and Uiseong seeds. Additionally, the interaction between the area of the suction surface and the curvature of the suction surface had some effects on the multiple rate for the Uiseong seeds. It was concluded that the guide height should be 4 mm or higher so that more than 90% of the metering rate could be achieved for the tested garlic seeds with the developed metering device.

• Korean Journal of Ecology and Environment
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• v.54 no.1
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• pp.71-77
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• 2021

Using data from flies collected with pitfall traps in 365 forests on a nationwide scale in Korea, the abundance and distribution changes of two families (Phoridae and Lauxaniidae) in Korean forests were predicted at the genus level according to two climate change scenarios: RCP 4.5 and RCP 8.5. The most suitable temperature for the 17 major genera was estimated using a weighted average regression model. Stichillus and Anevrina displayed the lowest optimum temperature with 7.6℃ and 8.5℃ in annual mean temperature, respectively, whereas Chonocephalus had the highest optimum temperature with 12.1℃. Among thirty genera, seven genera (four from Phoridae and three from Lauxaniidae), which showed their abundance in a bell-type or linear pattern along the temperature gradient, were used for predicting the distribution changes according to the future climate change scenarios. All the taxa of this study are expected to decrease in abundance and distribution as a function of temperature increase. Moreover, cold-adapted taxa were found to be more affected than warm-adapted taxa.

• ALGAE
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• v.31 no.3
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• pp.231-241
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• 2016

We examined the genetic diversity and abundance of picocyanobacteria using barcoded amplicon sequencing approaches and flow cytometry in the East Sea and the East China Sea to determine the distribution patterns of diversity during spring in seas adjacent to the Korean Peninsula. Synechococcus clades I and IV, which have been known as cold-adapted ecotypes, dominated at most stations. However, the relative abundances of the two dominant clades differed in their spatial patterns. Clade I was exclusively dominant in the shelf area of the East China Sea and the north East Sea. However, the dominant genotypes belonging to clade I had different spatial distributions in the two areas and responded oppositely to seawater temperature. The dominance of distinct genotypes under the different ecological conditions suggests the presence of ecologically different ecotypes within the clade. Abundances of clade IV were greater than those of clade I at most stations in the southwest East Sea, showing an apparently different pattern from that of the other areas. A warm-water adapted clade II was observed at significant levels only at stations located in the eastern East China Sea affected by a branch of the warm Kuroshio Current. These results suggest that the physicochemical properties of influencing water masses play an important role in determining the distribution of Synechococcus genotypes.

• Food Science and Biotechnology
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• v.17 no.6
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• pp.1391-1395
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• 2008

Leuconostoc citreum HJ-P4 is a strain isolated for kimchi fermentation with its low temperature-adapted growth feature and its high dextransucrase activity. The detailed characteristics of cell growth and dextran sucrase activities were investigated at various environmental conditions such as temperatures, pHs, salts, and raw ingredients. This strain showed almost 2-fold higher maximal cell concentration ($X_{max}$) than that of the type culture Leuconostoc mesenteroides B-512F at $10^{\circ}C$. The $X_{max}$ of the strain was maximum at pH 7 and the cell growth was inhibited by salts in a dose-dependent mode up to 7%. Addition of pepper (<6%), garlic (<10%), and ginger (<2%) in kimchi gave no inhibition effect on the growth of HJ-P4. Dextransucrase synthesized by this strain retained over 80% of its maximum activity at $10^{\circ}C$ showing a comparable cold-adapted feature to its host microbe. This culture can be used as a starter culture in the industrial kimchi production giving desirable functions and predominance at low temperature.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.730-735
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• 2003

The effect of pathogenicity on trehalose accumulation was studied using six species of entomopathogenic nematodes adapted to either cold or warm temperature conditions. Also, trehalose accumulation was studied by treating nematodes with glycerol or oil at trehalose concentration and mortality of cold temperature $condition(5^{\circ}C)$, S. longicaudum Gl was increased 50% than control. And at trehalose concent and mortality of warm temperature $condition(30^{\circ}C)$, S. glaseri Agr B3 was increased 45%. Intracellular trehalose accumulation was increased with treatment with 20% glycerol or oil. As the final results, the pathogenic duration of nematodes was increased as intracellular trehalose accumulation was increased

• Korean Journal of Microbiology
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• v.55 no.1
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• pp.83-85
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• 2019

Pseudomonas sp. PAMC 29040 was isolated from a maritime tundra soil in Antarctica for its ability to degrade lignin and subsequently confirmed to be able to depolymerize heterogeneous humic substance (HS), a main component of soil organic matter. The draft genome sequences of PAMC 29040 were analyzed to discover the putative genes for depolymerization of polymeric HS (e.g., dye-decolorizing peroxidase) and catabolic degradation of HS-derived small aromatics (e.g., vanillate O-demethylase). The information on degradative genes will be used to finally propose the HS degradation pathway(s) of soil bacteria inhabiting cold environments.

• Animal Systematics, Evolution and Diversity
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• v.11 no.2
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• pp.159-166
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• 1995

Mature larva and male and female adults of an ephemerid mayfly (Ephemeroptera : Ephemeridae) ,Ephemera separigata n.sp., were described from Korea. Larvae and adults of the species were distinguished from other Ephemera spp. by a apir of narrow and laterally oriented longitudinal stripses on the abodminal terga 7-9 . Larvae of the species adapted to cold water high mountain torrents(altitude 500-700m) where substrates were sand, gravel, and a large portion of pebbles and cobbles. Altitudinal adaptations between Korean Ephemera spp. were briefly discussed.

• Proceedings of the Korea Water Resources Association Conference
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• 2005.05b
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• pp.245-249
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• 2005

The numerical simulation of a two-phase flow In a discrete fracture network model is presented in this paper, The purpose of this work is to consider density-driven flows induced by the density difference between hot autochthonous heavy brines and injected cold water. Mechanical consequences of high pressure waves on the fracture permeability and heat exchanges between fluids and rock matrix are neglected in this study. The finite volume method is employed to discretize spatially and the system is solved by using an IMPES(Implicit Pressure-Explicit Saturation) scheme. In order to solve the strong non-linearity of the system, the Newton-Raphson algorithm is used. The well-known Buckeley-Leverett problem is adapted to validate results calculated from the model and a relatively good agreement is obtained.

• Nuclear Engineering and Technology
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• v.28 no.4
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• pp.405-414
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• 1996

In this paper, the unsteady 2-dimensional turbulent flow model for thermal stratification in a pressurizer surge line of PWR plant is proposed to numerically investigate the heat transfer and flow characteristics. The turbulence model is adapted to the low Reynolds number K-$\varepsilon$ model (Davidson model). The dimensionless governing equations are solved by using the SIMPLE (Semi-Implicit Method for Pressure Linked Equations) algorithm. The results are compared with simulated experimental results of TEMR Test. The time-dependent temperature profiles in the fluid and pipe nil are shown with the thermal stratification occurring in the horizontal section of the pipe. The corresponding thermal stresses are also presented. The numerical result for thermal stratification by the outsurge during heatup operation of PWR shows that the maximum dimensionless temperature difference is about 0.83 between hot and cold sections of pipe well and the maximum thermal stress is calculated about 322MPa at the dimensionless time 28.5 under given conditions.