• Journal of Veterinary Clinics
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• v.26 no.5
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• pp.445-449
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• 2009

For human organ transplantations, histidine-tryptophan-ketoglutarate solution (HTKS) and University of Wisconsin solution (UWS) have been shown to engender similar outcomes as gold standard cold preservation solutions ($4^{\circ}C$). To select the effective preservation solution for cold storage of kidney xenografts in miniature pig, which could be a potential source animal of bio-organs, this study compared early histopathological outcomes of cold preservation injury using HTKS and UWS. Twelve miniature pigs weighing 25.6 to 34.7 kg were divided into two groups (n = 6 per group), UWS group and HTKS group. The kidneys in each group were harvested, cold flushed, and preserved for 0, 24, 48, and 72 hrs at $4^{\circ}C$ with UWS or HTKS, respectively. Histolopathological examinations were assessed on kidney biopsy specimens, taken after each cold storage. The degree of renal injury was scored using 5 different criteria (pyknotic nuclei, disruption of cytoplasm, detachment of epithelium, loss of microvilli, tubular necrosis and loss of glomerular tufts) of the cellular components of the tissue. The degree of kidney damage was increased with prolonged cold ischemia time. UWS and HTKS have at least similar efficacy in kidney preservation within 24 hrs cold preservation time. However, in HTKS group cold-induced injury started to be observed more than in UWS group after 48 hrs of cold storage. In conclusion, UWS and HTKS were equally effective for cold preservation of miniature pig kidney in early preservation times; however, UWS may be more effective at longer preservation times as compared to HTKS.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.9 no.6
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• pp.1882-1888
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• 2008

Effects of 1-MCP(1-methylcyclopropene) on the postharvest life and fruit quality of cucumber 'Baekdadaki' were investigated. Cucumber were held at room and cold chamber($10^{\circ}C$) treated with several concentrations of 1-MCP. Physico-chemical characteristics such as fresh weight and firmness were greatly varied during storage, and the treatment with I-MCP(500ppb) showed the minimal loss of the values at harvest. The peel b value representing a senescence process was higher investigated in 1-MCP(250 and 1,000ppb treatment, and led to the apparent yellowing and softening after cold storage for 15 days. 1-MCP(500ppb) showed lower decay rate and better marketability of cucumber than other treatments after cold storage for 15 days. The respiration rate was higher found in cucumber treated with 1-MCP(250ppb) than in other treatment. Ethylene evolution was most detected in cucumber treated with 1-MCP(1000ppb) after room storage for 6 days. 1-MCP did not affect the production of ethylene in cucumber during room and cold storage.

• Journal of Biosystems Engineering
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• v.25 no.2
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• pp.107-114
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• 2000

Low temperature storage method is to increase the value of agricultural products by reducing quality loss and regulate consignment time by controlling respiration rates of agricultural products. Respiration rate of agricultural products depends on several factors such as temperature, moisture, gas composition and a microbe inside the storage room. Temperature is the most important factor among these, which affects respiration rate and causes low or high temperature damage. Fuzzy logic was used to control the temperature of a storage room ,which uses information of uncertain facts and mathematical model for room temperature control . Room temperature was controlled better by using fuzzy logic control method rather than on-off control method. Refrigerant flow rates and temperature deviations were measured for on-off system using TEV(temperature expansion valve) and for fuzzy system using EEV(Electrical Expansion Valve) . Temperature of the Storage room was lowered faster by using fuzzy system than on -off system. Temperature deviation was -0.6~+0.9$^{\circ}C$ for on-off system and $\pm$0.2$^{\circ}C$ for fuzzy system developed. Temperature deviation and variation of temperature deviation were used as inout parameters for fuzzy system. The most suitable input and output value were found by experiment. Cooling rate of the storage room decreased while temperature deviation increased for the sampling time of 20 sec.

• Korean Journal of Medicinal Crop Science
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• v.26 no.5
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• pp.401-407
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• 2018

Background: In Korea, seeds of Panax ginseng C. A. Meyer need to be stored under cold temperature and high humidity condition for months to break physiological dormancy, making storage difficult until spring-sowing. This study was conducted to test the effects of seed storage conditions and seed treatment on the emergence of seedling after spring-sowing in a nursery greenhouse. Methods and Results: After dehiscence, endocarp dried seeds in mild or completely, and wet seeds were stored in $2^{\circ}C$ and $-3.5^{\circ}C$ during winter. Storage at $-3.5^{\circ}C$ resulted in a lower emergence rate (ER) than that at $2^{\circ}C$, and additional cold ($2^{\circ}C$) treatment before or after storage at $-3.5^{\circ}C$ increased the ER. Endocarp dehydration prevented pre-germination at $2^{\circ}C$ storage and increased the ER of seeds stored at $-3.5^{\circ}C$. ER was also dependent on the batch of seeds. However, seed treatments before sowing had only limited effects on ER. Root loss was the main reason for damping-off; prolonged cold storage of seeds increased damping-off, as the detection of pathogens was not high. Conclusions: This study showed that storage conditions such as temperature and moisture content of seeds, affect the ER after spring-sowing and vitality of seedlings, suggesting further attention on seed control for secure seedling stands after spring-sowing.

• Applied Biological Chemistry
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• v.41 no.3
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• pp.228-233
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• 1998

The storage life of satsuma mandarin(Citrus unshiu Marc. var. miyagawa) by the various pretreatment of temperatures; non-treated, room temperature, $10,\;20^{\circ}C$ and $35^{\circ}C$ were investigated. The pretreated citrus fruits were stored at $4^{\circ}C$, 85% relative humidity. Weight loss of citrus fruits by the pretreatment at $35^{\circ}C$ for 24 hrs was the lowest among that of others. Decay ratio of $35^{\circ}C$ pretreated fruits was increased at initial stages of storage, but was maintained low level after that, compared to other treatments. After 115 days storage, firmness of fruits was lowered by the softening, and decayed fruits were occurred increasingly. Ethylene evolution was increased between $55{\sim}65$ days after storage, and the amount was increasing rapidly after 115 days. It seemed to be derived from decayed fruits and physiological activities. $CO_2$ content in fruit was decreased at initial stages of storage, but was increased between $55{\sim}100$ days during storage periods. Acid content, soluble solids, total sugar and vitamin C were reduced gradually during cold storage, but the difference among treatments was not so great. Pretreated fruits at $35^{\circ}C$ for 24 hrs before cold storage was effective on preventing from weight loss and respiration ratio. Optimum storage period of early variety of Satuma mandarin was regarded for 100 days on the basis of appeareance and taste.

• Food Science of Animal Resources
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• v.27 no.3
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• pp.314-319
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• 2007

The aims of this work were to compare the effects of dehairing methods and sex on pork quality and cold storage stability. For this study, one half of40 pigs were dehaired by scalding in the same abattoir, and the other half by dehiding. Each treatment consisted of one half gilts and one half castrated male pigs. Meat quality traits were measured at 24 hr after slaughter, and thiobarbituric acid (TBA), metmyoglobin contents and total plate counts (TPC) were evaluated at 1, 7, 14 and 21 days of cold storage. The carcass weight and backfat thickness of pigs dehaired by scalding were significantly higher than those dehided (p<0.05). Compared to scalded carcasses, dehided carcasses had a lower longissimus dorsi muscle temperature (p<0.05). Furthermore, dehided carcasses had significantly lower drip loss than scalded carcasses (p<0.05). However, meat color parameters were not affected by the dehairing method. During cold storage, TBA values of scalded carcasses were slightly higher initially, whereas the dehided carcasses had higher TBA values at 21 days (p<0.05). The total plate counts (TPC) associated with scalding and dehiding treatment were not significantly different at any point during cold storage. The TPC exponentially increased in both scalded and dehided carcasses from 7 to 14 days of cold storage.

• Horticultural Science & Technology
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• v.19 no.2
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• pp.170-173
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• 2001

Postharvest diseases developed on harvested products result in a great economic loss. The objective of this research was to develop a chemical treatment to control major postharvest pathogens including Botrytis cinerea and Penicillium expansum in the cold storage room. Acetic acid ($2.4mg{\cdot}L^{-1}$, $4.8mg{\cdot}L^{-1}$) inhibits spore germination and mycelial growth of B. cinerea and P. expansum on PDA at room temperature ($25^{\circ}C$) and low temperature ($2^{\circ}C$). Fumigation of cold storage room with $SO_2$ ($5g{\cdot}m^{-3}$) gas or gaseous acetic acid ($4.8g{\cdot}m^{-3}$) prior to operation greatly reduced population of fungi in the cold storage room.

• Journal of Applied Biological Chemistry
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• v.43 no.4
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• pp.250-253
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• 2000

The effects of high relative humidity (RH) on the physicochemical properties and microbial activity of mature green tomatoes ('Dombito') during refrigerated storage were determined at three temperatures (5, 10, and $15^{\circ}C$) and four different RH levels (91, 94, 97, and 99%). At each temperature, the weight loss rates of tomatoes at different levels of RH were significantly (p<0.05) different from each other. For the samples stored at $10^{\circ}C$, the weight losses were generally higher than those for the samples at $15^{\circ}C$ within the same RH level (i.e., greater vapor pressure deficit). The color change rates ('a' value) showed positive slopes, indicating that the tomato color was changing from green to red. Neither bacteria nor fungi caused visible damages to the samples, and the microbial counts were below 650 colony forming units/$cm^2$ during the test period.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.7 no.3
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• pp.382-395
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• 1995

A numerical study has been performed on flow characteristics in a domestic refrigerator whose size is $540mm{\times}1,530mm{\times}680mm$, considering existence of a fan and evaporator. The flow field has been simulated with the low Reynolds number $k-\bar{\varepsilon}$ turbulent model and SIMPLE algorithm based on the finite volume method. The region of fan which makes driving force for cold air distribution was modeled as a region in which momentum sources are generated uniformly. The concept of the distributed pressure resistance was applied to describe the momentum loss from evaporator. The result showed that the rate of cold air distribution into freezing room and cold storage room was almost 7 : 3.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.28 no.3
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• pp.237-244
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• 2022

Onions are a significant crop in Korea, and cultivation is increasing every year along with high demand. Onions are planted in the fall and mainly harvested in June, the rainy season, therefore, physiological changes in onion bulbs during long-term storage might have happened. Onions are stored in cold room and at adequate relative humidity to avoid quality loss. In this study, bio-yield stress and weight loss were measured as the quality parameters of net packaged onions during 10 weeks of storage, and the storage environmental conditions are monitored using sensor networks systems. Quality estimation of net packaged onion during storage was performed using the storage environmental condition data through machine learning approaches. Among the suggested estimation models, support vector regression method showed the best accuracy for the quality estimation of net packaged onions.