• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.97-97
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• 2017

Plant genomes include heterochromatic loci that consist of repetitive sequences and transposable elements. LTR retrotransposon is the major class of transposons in advanced plants in terms of proportion in plant genome. The elements contribute not only to genome size but also to genome stability and gene expression. A number of cases have been reported transposon insertions near genic regions affect crop traits such as fruit pigments, stress tolerance, and yields. Functional LTR retrotransposons produce extrachromosomal DNA from genomic RNA by reverse transcription that takes place within virus-like-particles (VLPs). DECREASED DNA METHYLATION 1 (DDM1) plays important roles in maintaining DNA methylation of heterochromatin affecting all sequence contexts, CG, CHG, and CHH. Previous studies showed that ddm1 mutant exhibits massive transcription of retrotransposons in Arabidopsis, but only few of them were able to create new insertions into the genome. RNA-dependent RNA POLYMERASE 6 (RDR6) is known to function in restricting accumulation of transposon RNA by processing the transcripts into 21-22 nt epigenetically activated small interfering RNA (easiRNA). We purified VLPs and sequence cDNA to identify functional LTR retrotransposons in Arabidopsis ddm1 and ddm1rdr6 plants. Over 20 LTR copia and gypsy families were detected in ddm1 and ddm1rdr6 sequencing libraries and most of them were not reported for mobility. In ddm1rdr6, short fragments of ATHILA gypsy elements were detected. It suggests easiRNAs might regulate reverse transcription steps. The highest enriched element among transposon loci was previously characterized EVADE element. It has been reported that active EVADE element is more efficiently silenced through female germline than male germline. By genetic analyses, we found ddm1 and rdr6 mutation affect maternal silencing of active EVADE elements. DDM1-GFP protein accumulated in megaspore mother cell but was not found in mature egg cell. The fusion protein was also found in early embryo and maternal DDM1-GFP allele was more dominantly expressed in the embryo. We observed localization of DDM1-GFP in Arabidopsis and DDM1-YFP in maize and found the proteins accumulated in dividing zone of root tips. Currently we are looking at cell cycle dependency of DDM1 expression using maize system. Among 10 AGO proteins in Arabidopsis, AGO9 is specifically expressed in egg cell and shoot meristematic cells. In addition, mutation of AGO9 and RDR6 caused failure in maternal silencing, implying 21-22 nt easiRNA pathway is important for retrotransposon silencing in female gametophyte or/and early embryo. On the other hand, canonical 24 nt sRNA-directed DNA methylation (RdDM) pathways did not contribute to maternal silencing as confirmed by this study. Heat-activated LTR retrotransposon, ONSEN, was not silenced by DDM1 but the silencing mechanisms require RdDM pathways in somatic cells. We will propose distinct mechanisms of LTR retrotransposons in germline and somatic stages.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.3
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• pp.198-205
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• 1982

In 1980, rice was considerably damaged by abnormal low temperature. In this paper, to determine the effect of low temperature on the growth and nutrition of rice, the experiment were carried out: varietal response to low temperature in the region with different elevations. Regional differences of heading response to low temperature were observed among varieties. The difference of days between regions were bigger in tongil lines than Japonica lines. Especially, Milyang 42 and Hangangchalbeo might belong the cold suceptible group, since the varieties were severely delayed their heading in the high mountainous region as comparred to plain region. The delay of heading with low temperature was brought out grain sterility, and fertility and ripening ratio is influenced cold tolerance and elevations, and it's decreased yield. Varieties with higher grain sterility by low temperature have higher total nitrogen content, but tended to have lower potassium and phosphate contents in the flag leaf. High content of total nitrogen, low contents of potassium and silicate were observed in the sterilized grain chaff an the opposite result were noted in the ferilized grain chaff at the ripening stage. The results reveal that the balance of these mineral element may play an important role in ripening and possibly cold tolerance.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.4
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• pp.249-256
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• 2007

This experiment was conducted to compare the agronomic characteristics and productivity in introduced hairy vetch cultivars and developed new Korean Hairy vetch cultivars in two areas of the experimental field of Grassland and Forage Crops Division, National Institute of Animal Science from 2005 to 2006. The experiment was arranged in randomized complete block design with three replications. A total of 11 hairy vetch cultivars used in this study including 9 introduced cultivars (Penn-02, Sander, Latigo, Welta, Ostsaat, VV4712, Minnie, Barlosa and Capello) and 2 Korean cultivars (Cold green and Cheong pa). The early flowering varieties of Minnie, Barlosa, Capello and Cold green were grown as early maturity cultivars, while the late flowering varieties of Penn-02, Sander, Latigo, Welta, Ostsaat, VV4712 and Cheong pa were grown as medium and late maturity. The winter hardiness of hairy vetch was good except for Minnie, Barlosa and Capello of early maturity cultivars. In this study, dry matter yield of VV4712 was the highest of 11 hairy vetch cultivars. And dry matter yield of medium and late maturity vetch increased in the middle region while that of early maturity increased in the south. The new Korean cultivar, Cheong pa, as a medium and late maturity hairy vetch was not bad compared to introduced cultivars and Cold green as an early maturity hairy vetch was an excellent cultivar compared to introduced cultivars in both regions. Latigo in NDF (neutral detergent fiber) and Welta in ADF (acid detergent fiber) were lower than other cultivars, while Sander in IVDMD (in vitro dry matter digestibility) and CP (crude protein) content were higher than other cultivars. The results of this experiment indicated that the productivity of hairy vetch cultivars was highest in VV4712, and Cheong pa and Cold green of new Korean cultivar were the promising cultivars of hairy vetch in Korea.

• Journal of The Korean Society of Grassland and Forage Science
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• v.40 no.3
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• pp.156-160
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• 2020

This study was conducted at the National Institute of Animal Science from 2010 to 2017. As a variety that is sufficiently productive in the southern regions to replace imported varieties and sufficiently cold-resistant to be cultivated in the central-northern regions, "IR605" was developed and submitted to the Korea Seed & Variety Service in an application for protection. The novel Italian ryegrass variety "IR605" is a diploid with green leaves, a semi-erect growth habit before wintering, and an erect growth habit in the spring. "IR605" was a medium maturing variety with a heading date of around May 15th. "IR605" had a flag leaf width of 9.9 mm, flag leaf length of 26.7 cm, and plant length on the heading date of 100 cm, which was approximately 5 cm longer than "Kowinearly." The stem thickness and ear length of "IR605" are 0.08 mm thicker and 0.5 cm longer than those of "Kowinearly", respectively. The cold-resistance of "IR605" was weaker than that of "Kowinearly", but strong enough to be cultivated in Pyeongchang, Gangwon province. The dry matter yield of "IR605" (9,308 kg/hectare) was 20% higher than that of "Kowinearly", which was further pronounced in the southern region of Haenam, where there was a 52% increased (p < 0.05). The in vitro dry matter digestibility of "IR605" was 68.4% at which was slightly higher than that of "Kowinearly", The total digestible nutrients was 58.5%, which was slightly lower than "Kowinearly". Overall, the feed quality characteristics of "IR605" were similar to those of "Kowinearly".

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.sup3
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• pp.33-37
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• 2016

Skin cancer is one of the most common cancers worldwide, including in Iran. Variations in its incidence rate among geographical areas are due to various contributing factors. Since there has been a lack of studies on this topic in our country, the present spatial analysis of skin cancer incidence in Iran in 2009 was conducted using data from the cancer registry system for the country. The reported incidences of the disease were standardized on the basis of the World Health Organization population and the direct method. Then the data were inserted into the GIS software, and finally, using the analysis of hot spots (Getis-Ord Gi), high-risk areas were drawn. Provinces that were 1.9 SD higher or lower than the national average were considered hot spots or cold spots, with significance at the level of 0.05. In 2009, a total of 9,964 cases of skin cancer occurred, 3,696 in women and 6,268 in men (standardized incidence rates of 15.8 and 22.6, respectively). The results of the study showed that in men and women, the disease demonstrated high incidence in the central provinces and desert regions. In women, Yazd Province and in men, Qom Province had significant hot spots (p <0.05). While Isfahan, Markazi, Tehran and Kurdistan provinces were expected to be hot spots, the differences from the national average were not significant at the 0.05 level. As well, the provinces of Sistan Va Baluchistan, Kerman, and Hormozgan were identified as cold or low-risk disease regions (p <0.05). The central provinces of the country due to hot weather conditions, more solar radiation, and closer vicinity to the central desert of Iran demonstrated higher incidence rates for skin cancer, so further epidemiological studies into the etiology and early detection are essential in these areas.

• Journal of Korea Water Resources Association
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• v.46 no.3
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• pp.253-265
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• 2013

The objective of this study is to analyse the current climate zone applied by K$\ddot{o}$ppen climate classification and the future climate zone projected by the A2 scenario in Asia regions. The spatial and temporal variations of precipitation and temperature were also analyzed. As regards to the result of analysis on the variation of climate factor, temperature and precipitation will be increasing $4.0^{\circ}C$ and 12% respectively in the 2080s comparing with the reference period (1991~2010). Spatially, the range of temperature increase on the high latitude area is higher than that on the low latitude area. The precipitation will be increasing averagely in the overall area, but the spatial unequal distribution of precipitation will be intensified. At the result of the future climate zone, the area of warm climates will be increasing while the area of cold climates will be decreasing. In 2080s, the temperature will be increasing as much as 7.2% and 1.9% on the Tropical climates and Arid climates respectively, but it will be decreasing as -2.4%, -4.9% and -1.8% on the Warm temperate climates, Cold climates and Polar climates respectively. Furthermore, the part of Savannah climates and Desert climates will be mostly increasing. It is mainly caused by the temperature increase and desertification impact according to global warming.

• Journal of the Korean Geosynthetics Society
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• v.13 no.4
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• pp.97-107
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• 2014

Finite element modelling and analysis were conducted for the roll-type steel mats which were placed on loose sand and subjected to a standard truck wheel load in this study. The roll-type steel mats mean that the steel mats can be folded as a circle shape for the carrying to fields in cold regions where workability is limited and are developed for a rapid rehabilitation method for roadway across soft ground which is caused by thawing during the summer season in cold regions. The model is composed of link elements to simulate nonlinear behavior of connections between steel mats, thick shell elements to have flexural stiffness of the steel mats, and springs to simulate characteristics of foundation soils. The structural behaviors of the shell, link elements, and springs were verified at each modelling step through experiment and analysis. Beam and shell analysis without the link elements were conducted and compared to results obtained from the model presented in this study. Significant vertical displacement is shown in the shell model with hinge connections. Therefore, the results demonstrate that the analysis model for the roll-type steel mats on loose sand needs further detail parametric studies.

• Journal of the Economic Geographical Society of Korea
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• v.21 no.4
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• pp.321-334
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• 2018

There are many factors that cause uneven regional developments in the country and one of main factors is outflow of regional income or products. The purpose of this study is to analyze regional production runoff by comparing GRDP and GRNI in basic local governments level. In this study, GRNI of basic local governments are estimated by local income tax data, The results of the study are as follows. Firstly, GRNI is more concentrated than GRDP. The analysis of Moran I showed that the spatial autocor-relation of GRNI is more distinct than that of GRDP. Local Moran I analysis shows that spatial hot spots and cold spots are more apparent in GRNI than GRDP. Secondly, the outflows of GRDP into a small number of regions are apparent. In about 80% of basic local governments, the net outflows of GRDP occur. The large net outflow regions are cities where manufacturing industry has developed and in the 20 lowest net outflow rate regions, 70-80% of GRDP outflows. The large net inflow regions are metropolitan area in Seoul and large local cities. Seocho-gu, Yongsan-gu, and Gangnam-gu in Seoul have a large net inflows and net inflow rates are over 90% of GRDP.

• The Transactions of the Korea Information Processing Society
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• v.3 no.7
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• pp.1669-1679
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• 1996

In this paper, we address the problem of how to replace huffers in multimedia database systems with time-varying skewed data access. The access pattern in the multimedia database system to support audio-on-demand and video-on-demand services is generally skewed with a few popular objects. In addition the access pattem of the skewed objects has a time-varying property. In such situations, our analysis indicates that conventional LRU(least Recently Used) and LFU(Least Frequently Used) schemes for buffer replacement algorithm(ABRN:Adaptive Buffer Replacement using Neural suited. We propose a new buffer replacement algorithm(ABRN:Adaptive Buffer Replacement using Neural Networks)using a neural network for multimedia database systems with time-varying skewed data access. The major role of our neural network classifies multimedia objects into two classes:a hot set frequently accessed with great popularity and a cold set randomly accessed with low populsrity. For the classification, the inter-arrival time values of sample objects are employed to train the neural network.Our algorithm partitions buffers into two regions to combine the best roperties of LRU and LFU.One region, which contains the 핫셋 objects, is managed by LFU replacement and the other region , which contains the cold set objects , is managed by LRUreplacement.We performed simulation experiments in an actual environment with time-varying skewed data accsee to compare our algorithm to LRU, LFU, and LRU-k which is a variation of LRU. Simulation resuults indicate that our proposed algorthm provides better performance as compared to the other algorithms. Good performance of the neural network-based replacement scheme means that this new approach can be also suited as an alternative to the existing page replacement and prefetching algorithms in virtual memory systems.

• Journal of the Korean Applied Science and Technology
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• v.36 no.4
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• pp.1410-1419
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• 2019

In this research, it is to find a method that frost does not form on the outdoor unit to develop a heat pump capable of heating in cold regions. For this reason, we produced an incubator capable of creating an environment of -25℃, and constructed an experimental apparatus so that experiments in the room were possible. However, it is necessary to grasp the characteristics of the air reaching the front of the heat pump outdoor unit installed in the experimental apparatus, and flow analysis was performed using ANSYS CFX, which is general-purpose software. As a result, the flow velocity of the air reaching the front of the outdoor unit in the outdoor unit chamber in the entire region of the simulation conditions (5.0 to 7.5 m/s) has many differences in the upper and lower portions, resulting in a natural state. It turned out that the condition can not be satisfied. Therefore, it is determined that it is necessary to additionally install a frequency divider at the front of the outdoor unit to make the flow velocity constant.