• Title/Summary/Keyword: coding sequences

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cDNA Cloning and Nucleotide Sequence Determination for VP7 Coding RNA Segment of Human Rotavirus Isolated in Korea (한국에서 분리된 사람 로타바이러스의 VP7 코딩 RNA 분절의 cDNA 합성과 염기서열 결정)

  • Kim, Young Bong;Kim, Kyung Hee;Yang Jai Myung
    • Korean Journal of Microbiology
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    • v.30 no.5
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    • pp.397-402
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    • 1992
  • The cDNA of RNA segment coding for VP7 of human rotavirus isolated from patient's stool at Seoul area was synthesized, amplified by polymerase chain reaction, field in with Klenow fragment of DNA polymerase I and cloned into pUC19. The cDNA sequence was determined and compared with that of VP7 coding RNA segments of group A rotaviruses isolates in foreign country. Over 90% sequence homology was found with serotyppe I sepcific WA1 and RE9 strains. Comparative analysis of the deduced amino acid sequences within the two variable regions (amino acid residue 87 through 101 and 208 through 221) with WA1 and RE9 strains also showed high degree of sequence similarity with each other.

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Joint Spatial-Temporal Quality Improvement Scheme for H.264 Low Bit Rate Video Coding via Adaptive Frameskip

  • Cui, Ziguan;Gan, Zongliang;Zhu, Xiuchang
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.6 no.1
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    • pp.426-445
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    • 2012
  • Conventional rate control (RC) schemes for H.264 video coding usually regulate output bit rate to match channel bandwidth by adjusting quantization parameter (QP) at fixed full frame rate, and the passive frame skipping to avoid buffer overflow usually occurs when scene changes or high motions exist in video sequences especially at low bit rate, which degrades spatial-temporal quality and causes jerky effect. In this paper, an active content adaptive frame skipping scheme is proposed instead of passive methods, which skips subjectively trivial frames by structural similarity (SSIM) measurement between the original frame and the interpolated frame via motion vector (MV) copy scheme. The saved bits from skipped frames are allocated to coded key ones to enhance their spatial quality, and the skipped frames are well recovered based on MV copy scheme from adjacent key ones at the decoder side to maintain constant frame rate. Experimental results show that the proposed active SSIM-based frameskip scheme acquires better and more consistent spatial-temporal quality both in objective (PSNR) and subjective (SSIM) sense with low complexity compared to classic fixed frame rate control method JVT-G012 and prior objective metric based frameskip method.

A deblocking filer for block-based compressed video sequences (블럭 기반으로 압축된 동영상을 위한 블럭화 현상 제거 기법)

  • 김성덕;이재연;라종범
    • Journal of the Korean Institute of Telematics and Electronics S
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    • v.35S no.2
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    • pp.89-96
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    • 1998
  • Conventional block-based video coders induce annoying blocking artifacts in very low bitarte coding. We propose a delocking filter which is appropriate for real time operation in a conventional video decoder. The proposed algorithm uses on dimensional filtering across block boundaries horizontally and vertiaclly with two separate filtering modes. The mode decision is quite simple but is fully based on the characteristics of human visual system and video sequences. In flat regions, a strong smoothing filter is appliced; and in the other regions, a moew sophisticated smoothing filter, which is based on the frequency information around block boundaries, is used to reduce blocking artifacts without introuducing undesired blur. Eeven though the proposed deblocking filter is quite simple, simulation results show that it improves both subjective and objective image quality for various image features.

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The Grammatical Structure of Protein Sequences

  • Bystroff, Chris
    • Proceedings of the Korean Society for Bioinformatics Conference
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    • 2000.11a
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    • pp.28-31
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    • 2000
  • We describe a hidden Markov model, HMMTIR, for general protein sequence based on the I-sites library of sequence-structure motifs. Unlike the linear HMMs used to model individual protein families, HMMSTR has a highly branched topology and captures recurrent local features of protein sequences and structures that transcend protein family boundaries. The model extends the I-sites library by describing the adjacencies of different sequence-structure motifs as observed in the database, and achieves a great reduction in parameters by representing overlapping motifs in a much more compact form. The HMM attributes a considerably higher probability to coding sequence than does an equivalent dipeptide model, predicts secondary structure with an accuracy of 74.6% and backbone torsion angles better than any previously reported method, and predicts the structural context of beta strands and turns with an accuracy that should be useful for tertiary structure prediction. HMMSTR has been incorporated into a public, fully-automated protein structure prediction server.

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Three-Dimensional Subband Coding of Video using Wavelet Packet Algorithm (웨이브릿 패킷 알고리즘을 이용한 3차원 비디오 서브밴드 코딩)

  • Chu, Hyung Suk;An, Chong Koo
    • The Transactions of the Korean Institute of Electrical Engineers D
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    • v.54 no.11
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    • pp.673-679
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    • 2005
  • This Paper presents the 3D wavelet transformation based video compression system, which possesses the capability of progressive transmission by increasing resolution and increasing rate for multimedia applications. The 3D wavelet packet based video compression system removes the temporal correlation of the input sequences using the motion compensation filter and decomposes the spatio-temporal subband using the spatial wavelet packet transformation. The proposed system allocates the higher bit rate to the low frequency image of the 3D wavelet sequences and improves the 0.49dB PSNR performance of the reconstructed image in comparison with that of H.263. In addition to the limitation on the propagation of the motion compensation error by the 3D wavelet transformation, the proposed system progressively transmits the input sequence according to the resolution and rate scalability.

Molecular Cloning and Characterization of Neuronal $\beta$-subunit of Large-Conductance$Ca^{2+}$-activated $K^+$ Channels from Rat Brain

  • Heo, Moon-Sun;Ha, Tal-Soo;Park, Chul-Seung
    • Proceedings of the Korean Biophysical Society Conference
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    • 2001.06a
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    • pp.38-38
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    • 2001
  • We cloned the cDNA encoding the neuron-specific $\beta$-subunit ($\beta$4) of large-conductance calcium-activated potassium channels from rat brain and determined the DNA sequences of the entire coding region (GenBank accession; AY028605). The deduced amino acid sequences of r$\beta$4, 210 amino acids in length, are closely related to the $BK_{Ca}$ $\beta$4 subunits of other species but show only limited sequence homology to other $\beta$-subunits, $\beta$1-$\beta$3.(omitted)d)

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Draft genome sequence of Lactobacillus reuteri KLR3004 from a fattening pig (비육돈 분변으로부터 분리한 Lactobacillus reuteri KLR3004 유산균주의 유전체 분석)

  • Park, Jongbin;Lee, Jun-Yeong;Jin, Gwi-Deuk;Kim, Eun Bae
    • Korean Journal of Microbiology
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    • v.53 no.2
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    • pp.146-148
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    • 2017
  • We sequenced the genome of Lactobacillus reuteri KLR3004 strain isolated from a fattening pig in South Korea. The sequences were assembled into a draft genome containing 1,996,237 bp with a G+C content of 38.75% and 1,837 predicted protein-coding sequences in 149 contigs.

Proteins Heading for the Chloroplast (엽록체로 향하는 단백질)

  • 홍주태
    • Journal of Plant Biology
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    • v.33 no.1
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    • pp.81-84
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    • 1990
  • The chloroplast has been the prime light-energy harvesting organelle on earth. It also carries out several key metabolic processes, such as lipid synthesis and nitrogen metabolism. Even though the chloroplast has its own genome, its coding capacity can afford only dozens of proteins, and most of the proteins functioning in the chloroplast are imported from the cytosol where nuclear encoded chloroplast genes are synthesized on free cytosokic ribosomes. Precursor proteins synthesized on cytosolic ribosomes have transit peptides at the amino termini of the proteins, and the transit peptide is sufficient to transfer chloroplast proteins from the cytosol into the chloroplast. When comparing amino acid sequences duduced from the nucleotide sequences of the clones of the chloroplast proteins, high homologies can be found among the transit peptides of proteins with the same function. Overall amino acid compositions of the transit peptides show amphiphilic characters of the transit peptides, and the amphiphilicity indicates that three dimensional structure of the transit peptide is responsible for the translocation of the chloroplast proteins.

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Comparative Plastome Sequences of Corchoropsis Siebold & Zucc.

  • Yu Gyeom Kim;Joonhyung Jung;Joo-Hwan Kim
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.70-70
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    • 2022
  • Corchoropsis Siebold & Zucc. was traditionally included in Tiliaceae, however, several morphological characters and molecular phylogenetic studies supported that it was the member of Dombeyoideae (Malvaceae). It is used as compost, medicinal, and ornamental herbs. Out of total three species, two species and one variety, grow in Korea. Although it is an East Asian endemic genus, plastome data has not been defined. Here, we characterize the plastome sequences of C. tomentosa (160,093 bp) and C. tomentosa var. psilocarpa (160,724 bp). Corchoropsis encodes 78 protein-coding genes, 30 tRNAs, and four rRNAs with a pseudogene inf A. Phylogenetic study revealed that Tilioideae is sister of Dombeyoideae. This study will contribute to define the genome structures, phylogenetic, and evolutionary studies of the genus Corchoropsis.

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A DCT-Domain Pre-filtering Scheme in a Video Encoder (동영상 부호화기 내부에서의 효과적인 DCT영역 전처리 필터링)

  • Kim, Sung-Deuk;Ra, Jong-Beom
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.37 no.5
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    • pp.42-53
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    • 2000
  • Efficient implementation of pre-filtering has been an important issue in video sequence coding, because it can remove camera noise and improve coding efficiency dramatically This paper introduces a novel pre-filtering scheme that is performed inside a video encoder The proposed pre-filtering is based on the approximated generalized Wiener filtering and two-dimensional discrete cosine transform (DCT) factorization. and is achieved by scaling the DCT coefficients of original image blocks for intra block coding and those of motion-compensated error blocks for inter block coding, respectively Even though the pre-filtering operation is embedded in a video encoder, its additional computational complexity is marginal compared to the encoding process, and the overall architecture of the conventional video encoder is maintained In spite of its simplicity, the proposed pre-filtering scheme provides good filtering and coding performance for noisy video sequences.

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