• 한국지반공학회논문집
• /
• 제37권1호
• /
• pp.5-15
• /
• 2021

북극권과 같은 한대지역의 동상민감성 지반은 계절적 대기 온도 변화 및 설치된 구조물의 온도에 의해 지반의 융기 및 침하 문제가 존재한다. 이러한 동상민감성 지반의 융기 및 침하 방지를 위한 지반안정화 공법으로는 매립 및 치환공법, 열 사이펀 등이 존재한다. 여기서 열 사이펀이란 내부 냉매의 증발, 응축을 반복하며 열 순환을 통해 이상 유동(two-phase flow)의 형태로 냉매가 지반의 온도를 외부로 전달하여 지반 온도를 조절 할 수 있는 공법이다. 본 연구는 이러한 열 사이펀의 성능을 열전도율로 수치화하기 위하여 ABAQUS 내부의 User-subroutine 코딩을 통해 열 사이펀을 지중의 한 열원으로 간주, 경계조건으로 적용시켜 기존 문헌의 열 사이펀 실내모형실험의 온도분포 결과와 비교하여 산정하였다.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2022년도 추계학술대회
• /
• pp.214-214
• /
• 2022

Soluble starch synthases (SSs) elongate α-glucans from ADP-Glc to the glucan nonreducing ends and play a critical role in synthesizing resistant starch in the rice. A total of 10 SSs isoforms were reported in rice, including granules-bound starch synthase I (GBSSI), GBSSII, starch synthase I (SSI), SSIIa (SSII-3), SSIIb (SSII-2), SSIIc (SSII-1), SSIIIa (SSIII-2), SSIIIb (SSIII-1), SSIVa (SSIV-1), and SSIVb (SSIV-2). SSIII proteins are involved in forming the B chain and elongating cluster filling chains in amylopectin metabolism. The functions of SSIIIb (SSIII-1) are less clear as compared to SSs. Here, we sought to shed light on the genetic diversity profiling of the SSIII-1 gene in 374 rice accessions composed of 54 wild-type accessions and 320 bred cultivars (temperate japonica, indica, tropical japonica, aus, aromatic, and admixture). In total, 17 haplotypes were identified in the SSIII-1 coding region of 320 bred cultivars, while 44 haplotypes were detected from 54 wild-type accessions. The genetic diversity indices revealed the most negative Tajima's D value in the temperate-japonica, followed by the wild type, while Tajima's D values in other ecotypes were positive, indicating balancing selection. Nucleotide diversity in the SSIII-1 region was highest in the wild group (0.0047) while lowest in temperate-japonica. Lower nucleotide diversity in the temperate-japonica is evidenced by the negative Tajima's D and suggested purifying selection. The fixation index (F_ST) revealed a very high level of gene flow (low F_ST) between the tropical-japonica and admixture groups (F_ST=-0.21) followed by admixture and wild groups (-0.04), indica and admixture groups (0.02), while low gene flow with higher F_ST estimates between the temperate-japonica and aus groups (0.72), tropical-japonica and aromatic groups (0.71), and temperate-japonica and admixture groups (0.52). Taken together, our study offers insights into haplotype diversity and evolutionary fingerprints of SSIII-1. It provides genomic information to increase the resistant starch content of cooked rice.

• Korean Circulation Journal
• /
• 제54권5호
• /
• pp.233-252
• /
• 2024

Background and Objectives: Myocardial ischemia-reperfusion injury (MIRI) refers to the damage of cardiac function caused by restoration of blood flow perfusion in ischemic myocardium. However, long non-coding RNA prostate androgen regulated transcript 1 (PART1)'s role in MIRI remain unclear. Methods: Immunofluorescence detected LC3 expression. Intermolecular relationships were verified by dual luciferase reporter assay. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, flow cytometry and transferase-mediated dUTP nick-end labeling (TUNEL) assays analyzed cell viability and apoptosis. The release of lactate dehydrogenase was tested via enzyme-linked immunosorbent assay (ELISA). Left anterior descending coronary artery surgery induced a MIRI mouse model. Infarct area was detected by 2,3,5-triphenyltetrazolium chloride staining. Hematoxylin and eosin staining examined myocardial injury. ELISA evaluated myocardial marker (creatine kinase MB) level. Results: PART1 was decreased in hypoxia/reoxygenation (H/R) induced AC16 cells and MIRI mice. PART1 upregulation attenuated the increased levels of Bax, beclin-1 and the ratio of LC3II/I, and enhanced the decrease of Bcl-2 and p62 expression in H/R-treated cells. PART1 upregulation alleviated H/R-triggered autophagy and apoptosis via miR-302a-3p. Mechanically, PART1 targeted miR-302a-3p to upregulate transcription factor activating enhancer-binding protein 2C (TFAP2C). TFAP2C silencing reversed the protected effects of miR-302a-3p inhibitor on H/R treated AC16 cells. We further established TFAP2C combined to dual-specificity phosphatase 5 (DUSP5) promoter and activated DUSP5. TFAP2C upregulation suppressed H/R-stimulated autophagy and apoptosis through upregulating DUSP5. Overexpressed PART1 reduced myocardial infarction area and attenuated MIRI in mice. Conclusion: PART1 improved the autophagy and apoptosis in H/R-exposed AC16 cells through miR-302a-3p/TFAP2C/DUSP5 axis, which might provide novel targets for MIRI treatment.

• Clinical and Experimental Pediatrics
• /
• 제45권2호
• /
• pp.183-191
• /
• 2002

목 적 : 본 연구에서는 임상적으로 XLA로 진단받고 현재 치료 중인 한가족 3명 환아의 가계를 대상으로 말초혈액 단핵구의 Btk 단백질 발현과 Btk 유전자 변이를 분석하고자 하였다. 방 법 : 환아 말초혈액 단핵구의 Btk 발현도를 항 Btk 항체를 이용한 유세포계측을 통해 분석하고 PCR-SSCP 및 직접 염기서열 분석에 의해 Btk 유전자 변이를 분석하였다. 결 과 : 유세포 계측 및 PCR-SSCP에 의하여 가계내의 환자 3명 및 보인자 4명을 확인하였으며 환아들의 단핵구의 Btk 발현도를 조사한 결과 2.7% 미만으로 건강인에 비해 매우 유의한 감소를 나타내었다. 유전자 변이 분석 결과 Btk 유전자 exon 3에서 점돌연변이($T{\rightarrow}C$)가 발견되어 61번째 아미노산의 치환이 일어난 과오돌연변이를 확인할 수 있었다. 동일한 유전형을 지닌 3명의 환아들은 임상적으로 매우 다양한 표현형을 나타내었다. 특히 환아들의 혈청 IgG level이 낮을수록 임상 양상은 심한 소견을 보였다. 결 론 : 본 연구 결과를 종합해 볼 때 임상적으로 XLA로 진단된 환아와 가족에 대한 항 Btk 항체를 이용한 유세포계측 및 PCR-SSCP 방법은 XLA 환아 및 보인자의 진단에 매우 유용하며 신속하게 적용될 수 있는 기법으로 생각된다. 또한 분자유전학 기법을 이용하여 환아의 유전자 변이를 검색한 결과 Btk의 PH 도메인 내 한개의 과오돌연변이를 검색하여 XLA를 최종 확진할 수 있었다.

• ALGAE
• /
• 제29권1호
• /
• pp.15-25
• /
• 2014

The number of species of non-native and invasive marine algae is growing, with concomitant public concern about native ecosystems and coastlines. Gelidium vagum, recently introduced from northeast Asia to Europe and North America, commonly occurs from the intertidal to subtidal zones in Korea, China, and Japan. To investigate the level of genetic diversity of native populations, we analyzed mitochondrial cox1 and cob from 108 specimens of G. vagum from Korea, China, eastern Russia, including from the Netherlands and USA. The haplotype network of individual and cox1 + cob datasets revealed no genetic structure in local populations, suggesting genetic flow between Korean populations. Our results corroborate a typical pattern of genetic diversity for introduced species, with low levels in introduced populations and high levels in native populations. All haplotypes were shared between the Netherlands and USA, but not between Korea and the Netherlands / USA except cox1. Additional sampling will identify donor populations in native northeast Asian waters. This is the first report of the utility of the mitochondrial coding cob sequences in red algae.

• Journal of Microbiology and Biotechnology
• /
• 제15권5호
• /
• pp.1115-1119
• /
• 2005

The photoheterotrophic $H_2$ production of Rhodobacter sphaeroides was significantly increased through disruption of the genes coding for uptake hydrogenase and poly-${\beta}$-hydroxybutyrate (PHB) synthase (Lee et al., Appl. Microbiol. Biotechnol. 60: 147-153, 2002). In this work, we further removed the B800-850 light-harvesting (LH) complex from the strain and found an increase in $H_2$ production at the light-saturating cell growth (${\ge}10$ Watts $[W]/m^2$). Neither the mutant nor the wild-type produced more $H_2$ at the brighter light. Accordingly, light does not appear to be limited for the $H_2$ production by the presence of B800-850. However, increase in the level of the spectral complexes resulted in decrease of $H_2$ production. Thus, although the B875 is essential for light harvesting, the consumption of cellular energy for the synthesis of B800-850 and the surplus LH complexes may reduce the energy flow into the $H_2$ production of R. sphaeroides.

• 전자공학회논문지S
• /
• 제34S권10호
• /
• pp.98-103
• /
• 1997

Given the small bit allocation for motion information in very low bit-rate coding, motion estimation using the block matching algorithm(BMA) fails to maintain an acceptable level of prediction errors. The reson is that the motion model, or spatial transformation, assumed in block matching cannot approximate the motion in the real world precisely with a small number of parameters. In order to overcome the drawback of the conventional block matching algorithm, several triangle-based methods which utilize triangular patches insead of blocks have been proposed. To estimate the motions of image sequences, these methods usually have been based on the combination of optical flow equation, affine transform, and iteration. But the compuataional cost of these methods is expensive. This paper presents a fast motion estimation algorithm using a multiple linear regression model to solve the defects of the BMA and the triange-based methods. After describing the basic 2-D triangle-based method, the details of the proposed multiple linear regression model are presented along with the motion estimation results from one standard video sequence, representative of MPEG-4 class A data. The simulationresuls show that in the proposed method, the average PSNR is improved about 1.24 dB in comparison with the BMA method, and the computational cost is reduced about 25% in comparison with the 2-D triangle-based method.

• 한국컴퓨터정보학회논문지
• /
• 제16권12호
• /
• pp.121-130
• /
• 2011

본 논문에서는 적극적인 저작권교육을 위해 초등학생들이 자주 접하는 디지털 저작물을 만드는 과정을 통한 교수방법을 설계하였다. 학습자가 창작자가 되어 게임을 소재로 한 프로그래밍을 통해 알고리즘과 순서도, 코딩을 통하여 창작의 노력을 깨닫게 하고 자신의 노력이 소중함을 표현하는 저작자 표시와 저작의 가치를 비용으로 책정하도록 하여 동료평가를 통해 그 가치를 평가받는 교수방법을 설계하였다. 또한, 현장에 적용하여 학생들의 내면의 변화를 관찰하여 그 효과를 분석하였다.

• 컴퓨터교육학회논문지
• /
• 제18권5호
• /
• pp.25-34
• /
• 2015

본 논문의 목적은 Computational Thinking(CT) 교육에서의 학습자 평가를 위한 방안으로 학습자가 제작한 프로젝트의 코드를 분석하는 방법을 제안한다. 최근 초중등 SW교육에서는 블록형 프로그래밍 도구를 활용한 교육이 이루지고 있으므로, 학생들의 산출물인 스크래치 프로젝트의 코드를 분석하여 CT 개념 습득과 학습자의 수준을 평가하는 방안을 모색하였다. 초보학습자 45명의 프로젝트를 분석한 결과 초보학습자들의 잘못된 습관에 대한 패턴이 나타났으며 CT 개념 학습을 코드의 패턴에 의해 평가할 수 있다는 것을 검증하였다. 학습자의 수준이 높을수록 논리적 사고, 동기화, 플로우 제어, 데이터 표현의 요소 점수가 높게 나타났다. 본 연구의 결과는 초중등 SW 교육에서 CT 개념 학습에 대한 평가를 위해 활용할 수 있다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권5호
• /
• pp.2983-2986
• /
• 2013

MicroRNAs (miRNAs) are small, non-coding RNAs (18-25 nucleotides) that post-transcriptionally modulate gene expression by negatively regulating the stability or translational efficiency of their target mRNAs. In this context, the present study aimed to evaluate the in vitro effects of miR-153 inhibition in the breast carcinoma cell line MDA-MB-231. Forty-eight hours after MDA-MB-231 cells were transfected with the miR-153 inhibitor, an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was utilized to determine the effects of miR-153 on cell viability. Flow cytometry analysis and assessment of caspase 3/7 activity were adopted to determine whether miR-153 affects the proliferation rates and apoptosis levels of MDA-MB-231 cells. Our results showed that silencing of miR-153 significantly inhibited growth when compared to controls at 48 hours, reducing proliferation by 37.6%, and inducing apoptosis. Further studies are necessary to corroborate our findings and examine the potential use of this microRNA in future diagnostic and therapeutic interventions.