• Journal of KIISE:Software and Applications
• /
• v.29 no.1_2
• /
• pp.98-113
• /
• 2002

In this paper, we present advanced algorithms to reduce the computations of block matching algorithms for motion estimation in video coding. Advanced multi-level successive elimination algorithms(AMSEA) are based on the Multi-level successive elimination algorithm(MSEA)[1]. The first algorithm is that when we calculate the sum of absolute difference (SAD) between the sum norms of sub-blocks in MSEA, we use the partial distortion elimination technique. By using the first algorithm, we can reduce the computations of MSEA further. In the second algorithm, we calculate SAD adaptively from large value to small value according to the absolute difference values between pixels of blocks. By using the second algorithm, the partial distortion elimination in SAD calculation can occur early. So, the computations of MSEA can be reduced. In the third algorithm, we can estimate the elimination level of MSEA. Accordingly, the computations of the MSEA related to the level lower than the estimated level can be reduced. The fourth algorithm is a very fast block matching algorithm with nearly 100% motion estimation accuracy. Experimental results show that AMSEA are very efficient algorithms for the estimation of motion vectors.

• The KIPS Transactions:PartB
• /
• v.10B no.1
• /
• pp.95-102
• /
• 2003

In surveillance video system, there are many classes of images and some spatial regions are more important than other regions. The conventional compression method in this system have been compressed there full frames without classfying them depend on their important parts. To improve the accuracy of the image coding and deliver effective compression for the surveillance video system, it was necessary to separate the regions according to their importance. In this paper, we propose a new effective surveillance video image compression method. The proposed scheme defines importance based three-level region of interest block in a frame, such as background, motion object block, and the feature object block. A captured video image frame can be separated to these three different levels of block regions. And depends on the priority, each block can be modified and compressed in different resolution, compression ratio and qualify factor. Therefore, in surveillance video system, this algorithm not only reduces the image processing time and space, but also guarantees the Important image data in high quality to acquire the system's goal.

• Proceedings of the KSRS Conference
• /
• 2000.04a
• /
• pp.166-171
• /
• 2000

밀티빔 음향 측심기 (Multibeam Echo Sounder)는 탐사선에 수직방향으로 해저면을 주사(Swath)하여, 한번의 송수신(Ping)으로 다중의 빔자료를 얻을 수 있는 측심기로, 해저면에 반사되어 되돌아오는 음파의 음압을 기록하고, 사이드 스캔 소나 자료도 동시에 취득하는 기능을 가지고 있으므로, 측심된 해저 지형(Bathymetry)과 해저 지형을 덮고 있는 해저면의 퇴적 상황(Sediment Environment)도 동시에 얻을 수 있는 다목적 측심기이다. 본 논문에서는 L3사의 Sea Beam 2100 멀티빔 음향 측심기를 통해 얻은 자료를 처리하여, 3차원 공간 데이터인 DEM(Digital Elevation Model)을 생성하고, VRML을 이용한 웹상에서의 해저 지형 가시화를 통해, 세계 어느 곳에서나 웹을 통하여 쉽게 정보를 공유할 수 있는 3차원 해저 지리 정보 시스템의 구현을 목적으로 한다. 멀티빔 음향 측심기를 통해 얻어진 자료는 항해 자료 보정, 음속 보정, 빔 좌표 계산과 분리, 오측심 자료 제거, 조석 보정 등의 단계를 거쳐 측심자료의 정확도 및 신뢰도를 높이는 과정을 거치게 된다. 보정된 멀티빔 음향 측심자료는 무작위 점 사상(Point Topology)으로 산재 되어 있는 빔 자료를 임의의 단위영역으로 변환하는 과정을 거쳐야 하는데, 이 과정을 격자화라고 한다. 자료의 격자화를 통해 3차원 공강 데이터인 DEM 파일을 제작하고, 이 DEM 파일과 음압 영상을 이용해 웹상에서의 3차원 해저 지형의 가시화를 실현한다. 웹상에서의 3차원 지형 가시화에서 방대한 양의 지형 데이터는 데이터 전송 시간과 렌더링 시간에 치명적인 문제이다. 따라서, 렌더링 시간과 데이터 전송 시간을 단축시키기 위한, 지형 자료의 LOD(Level of Detail)를 통해, VRML을 이용한 보다 효과적인 웹상에서의 3차원 해저 지형의 가시화를 실현한다.면 기업은 고객으로 공간적인 제약으로 인한 불신을 불식시키는 신뢰감을 주게 된다. 이러한 고객서비스 향상과 물류비용 절감은 사이버 쇼핑몰이 전국 어디서나 우리의 안방에서 자연스럽게 점할 수 있는 상황을 만들 것이다.SP가 도입되어, 설계업무를 지원하기위한 기본적인 시스템 구조를 구상하게 된다. 이와 함께 IT Model을 구성하게 되는데, 객체지향적 접근 방법으로 Model을 생성하고 UML(Unified Modeling Language)을 Tool로 사용한다. 단계 4)는 Software Engineering 관점으로 접근한다. 이는 최종산물이라고 볼 수 있는 설계업무 지원 시스템을 Design하는 과정으로, 시스템에 사용될 데이터를 Design하는 과정과, 데이터를 기반으로 한 기능을 Design하는 과정으로 나눈다. 이를 통해 생성된 Model에 따라 최종적으로 Coding을 통하여 실제 시스템을 구축하게 된다.the making. program and policy decision making, The objectives of the study are to develop the methodology of modeling the socioeconomic evaluation, and build up the practical socioeconomic evaluation model of the HAN projects including scientific and technological effects. Since the HAN projects consists of 18 subprograms, it is difficult In evaluate all the subprograms simultaneously. Despite, each program is being performed under the category of HAN projects, so the common soci

• Korean Journal of Weed Science
• /
• v.31 no.4
• /
• pp.323-329
• /
• 2011

We investigated photodynamic stress-induced antioxidant responses in transgenic rice overexpressing Bradyrhizobium japonicum 5-aminolevulinic acid synthase (ALA-S) coding sequence lacking plastidal transit sequence. High light of $350{\mu}mol\;m^{-2}\;s^{-1}$ decreased the quantum yield in the transgenic lines, C4 and C5, compared to that of wild-type line. By contrast, non-photochemical quenching (NPQ) levels of C4 and C5 under high light were higher than those of the transgenic lines under low light of $150{\mu}mol\;m^{-2}\;s^{-1}$ as well as wild-type line under low and high light. Greater levels of NPQ in the transgenic lines exposed to high light were in a close correlation with increases in the xanthophyll pigment, zeaxanthin. Under high light, levels of neoxanthin, violaxanthin, lutein, and ${\beta}$-carotene in the transgenic lines were lower than those in wild-type line. Taken together, nonphotochemical energy dissipation and photoprotectant xanthophyll pigments play a critical role to deal with the severe photodynamic damage in the transgenic rice plants, although they could not overcome the photodynamic stress, leading to severe photobleaching symptoms.

• Korean Journal of Microbiology
• /
• v.32 no.2
• /
• pp.120-125
• /
• 1994

The genes, trpB, trpA and 3’ trpC(F) of Vibrio metschnikovii strain RH530 were cloned and sequenced. The trpB and trpA genes had open reading frames of 1,173 bp and 804 bp encoding 391 and 268 amino acids, respectively. The trpB and trpA genes had conventional ribosome-binding sequences and overlapped with each other by one nucleotide, suggesting that these two genes are translationally coupled. 115 nucleotide upstream the trpB start codon, tjere was an incomplete open reading frame of the 3’-end of the trpC(F). The amino acid sequences of trpB, trpA and trpC(F) of V. metschnikovii RH530 had identities of 64.2%, 82.4% and 73.7% respectively, for those of V. parahaemolyticus; 58.7%, 72.3% and 54.9%, respectively, for Salmonella typhimurium; and 42.6%. 54.1% and 12.5%, respectively, for brevibacterium lactofermentum. The genetic organization of these genes, especially in the noncoding region between trpC(F) and trpB, was distinct from that of Enterobacteriaceae.

• Korean Journal of Microbiology
• /
• v.54 no.3
• /
• pp.289-292
• /
• 2018

Using pyrene as the enrichment nutrient, a bacterial strain 10PY1A, was isolated by enrichment culture from oil-contaminated sea sand of Arabian Gulf in Saudi Arabia, and this strain belongs to the species Idiomarina piscisalsi, based on 16S RNA gene sequence analysis. The genome of I. piscisalsi strain 10PY1A contains 2,346 protein-coding sequences and an average GC content of 47.4% in its chromosome (2.59 Mbp). Genes encoding proteins related to the degradation of pyrene were existed in the strain 10PY1A genome, indicating that this strain can be used to degrade polycyclic aromatic hydrocarbons in oil-contaminated marine flora and soil.

• Journal of Preventive Medicine and Public Health
• /
• v.26 no.2 s.42
• /
• pp.293-309
• /
• 1993

With expanded and extended coverage of the national medical insurance and fast growing health care expenditures, appropriateness of health service utilization and quality of care are concerns of both health care providers and insurers as well as patients. An accurate patient classification system is a basic tool for effective health care policies and efficient health services management. A classification system applicable to Korean medical information-Korean Diagnosis Related Groups (K-DRGs)-was developed based on the U.S. Refined DRGs, and the performance of the developed system was assessed in this study. In the process of the development, first the Korean coding systems for diagnoses and procedures were converted to the systems used in the definition of the U.S. Refined DRGs using the mapping tables formulated by physician panels. Then physician panels reviewed the group definition, and identified medical practice patterns different in two countries. The definition was modified for the differences in K-DRGs. The process resulted in 1,199 groups in the system. Several groups in Refined DRGs could not be differentiated in K-DRGs due to insufficient medical information, and several groups could not be defined due to procedures which were not practiced in Korea. However, the classification structure of Refined DRGs was retained in K-DRGs. The developed system was evaluated fur its performance in explaining variations in resource use as measured by charges and length of stay(LOS), for both all and non-extreme discharges. The data base used in this evaluation included 373,322 discharges which was a random sample of discharges reviewed and payed by the medical insurance during the five-month period from September 1990. The proportion of variance in resource use which was reduced by classifying patients into K-DRGs-r-square-was comparable to the performance of the U.S. Refined DRGs: .39 for charges and .25 for LOS for all discharges, and .53 for charges and .31 for LOS for non-extreme discharges. Another measure analyzed to assess the performance was the coefficient of variation of charges within individual K-DRGs. A total of 966 K-DRGs (87.7%) showed a coefficient below 100%, and the highest coefficient among K-DRGs with more than 30 discharges was 159%.

• Proceedings of the Korean Society of Life Science Conference
• /
• 2001.06a
• /
• pp.67-86
• /
• 2001

A strain producing strongly fibrinolytic enzyme was isolated from soil and was identified to be Bacillus subtilis by biochemical and physiological characterization. The optimal culture conditions for the production of fibrinolytic enzyme was determined to be 1.0% tryptone, 1.5% soluble starch, 0.5% Peptone, 0.5% NaCl, $(NH_{4})_{3}PO_4.3H_{2}O, and MgSO_{4}.7H_{2}O.$ Initial pH and temperature were pH 8.0 and $30^{\circ}C$ , respectively, The highest enzyme production was observed at 30 hours of cultivation at $30^{\circ}C$ The fibrinolytic enzyme was purified to homogeneity by DEAE Sephadex A-50 ion exchange column chromatography, 70% ammonium sulfate precipitation, Sephadex G-200 and G-75 gel filtration column chromatography. The molecular weight of the purified enzyme was 28,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A gene encoding the fibrinolytic enzyme was cloned into a plasmid vector pBluescript, transforming E.coli XL-1 Blue. The clone was able to degrade fibrin, This indicated that the gene could encode a fibrinolytic enzyme. The nucleotide sequence of the 2.7 kb insert was determined in both direction. One open reading frame composed of 1023 nucleotides was found to be a potential protein coding region. There was the putative Shine-Dalgano sequence and TATA box upstream of the open reading frame. The homology search data in the genome database showed that both the 2.7 kb insert and 1 kb open reading frame carried no significance in the nucleotide sequence of known fibrinolytic enzyme from Bacillus serovars. The recombinant cell harboring the novel gene involved in fibrinolysis was subjected to protein purification. The molecular mass of the purified fibrinolytic enzyme was determined to be 31864 Dalton, which was highly in accordance with the molecular mass(33 kDa) of the fibrinolytic gene deduced from the insert. The fibrinolytic enzyme was Purified 50.5 folds to homogeneity in overall yield of 10.7% by DEAE Sephadex A-50 ion exchange, 85% ammonium sulfate precipitation, Sephadex G-50, Superdex 75 HR FPLC gel filtration. In conclusion, a novel fibrinolytic gene from Bacillus subtilis was identified and characterized by cloning a genomic library of Bacillus subtilis into pBleuscript. For the soybean fermented by this strain, it is found that there increased assistant protein about 20% compared to the soybean not fermented and increased about 30% according to amino acid analysis and, in particular, essential amino acid increased about 40%. When keeping this fermented soybean powder at room temperature for about 70days, it showed very high stability maintaining almost perfect activity and, therefore, it gave us great suggestion its possibility of development as a new functional food.

• Molecules and Cells
• /
• v.37 no.3
• /
• pp.213-219
• /
• 2014

MicroRNAs (miRNAs) represent a class of small non-coding regulatory RNAs that play important roles in normal hematopoiesis, including erythropoiesis. Although studies have identified several miRNAs that regulate erythroid commitment and differentiation, we do not understand the mechanism by which the crucial erythroid transcription factors, GATA-1and NF-E2 directly regulate and control differentiation via miRNA pathways. In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. Furthermore, the increase of miR-199b-5p in erythroid cells occurred in a GATA-1- and NF-E2-dependent manner during erythrocyte maturation. Both GATA-1 and NF-E2 bound upstream of the miR-199b gene locus and activated its transcription. Forced expression of miRNA-199b-5p in K562 cells affected erythroid cell proliferation and maturation. Moreover, we identified c-Kit as a direct target of miR-199b-5p in erythroid cells. Taken together, our results establish a functional link among the erythroid transcription factors GATA-1/NF-E2, miR-199b-5p and c-Kit, and provide new insights into the coupling of transcription and post-transcription regulation in erythroid differentiation.

• Journal of Life Science
• /
• v.12 no.1
• /
• pp.96-105
• /
• 2002

To investigate expression of the artificial gene encoding a repeated tripeptide lysyl-glutamyl-tryptophan in tobacco plant, the plant binary vector, pART404 has been constructed, which contains the duplicated CaMV 35S promoter, an artificial gene coding for repetitive polymer (Lys-Glu-Trp)$_{64}$, and nopaline synthase (nos) terminator. The recombinant expression vector was introduced in Nicotiana tabacum (var. Xanthi) via Agrobacterium tumefaciens-mediated trans-formation. The transgenic calli selected by kanamycin containing medium were then regenerated to whole plants. Southern blot analysis indicated that five transgenic plants (No. 1, 7, 9, 43, 45) showed the hybridizing signals at 1.1 kb of the expected size on EcoRI digestion and each of the transgenic plants contained 1 or 3 copies of the artificial gene inserted into its genome. By northern blot analysis, the size of the hybridized total RNA was estimated to be approximately 1.2 kb and the RNA appeared generally to have the integrity. Western blot indicated that the protein was detected at the position of 33 kDa and the expression level of the polypeptide in the transgenic plant (No. 45) was measured to approximately 0.1% of the total protein.