• 한국물환경학회지
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• 제26권1호
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• pp.52-60
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• 2010

We investigated temporal changes of growth of hydrophytes, aquatic biota and absorption of nutrient depending on planting mat type (synthetic fiber, coconut fiber) of artificial vegetation island (AVI) through pilot test using AVI miniatures in Kyungan Stream area of Lake Paldang. There were not significant differences of the water quality parameters (DO, pH, conductivity, turbidity, temperature), phytoplankton and zooplankton abundance among AVI miniatures and control station. The benthic macroinvertebrates showed most individual numbers in the miniature which was made by synthetic fiber and planted with Phragmites australis. The average and maximum height of hydrophytes in AVI miniatures was similar except one miniature where Phragmites australis was planted in synthetic fiber mat and grew more slowly. The adsorbed amount of nutrients and microbes in coconut fiber mat were larger than those in synthetic fiber mat regardless of trophic state of installed waterbody. The continuous increase of adsorbed amount of nutrients and microbes of coconut fiber mat for 8 months in an oligotrophic lake indicates that coconut fiber mat is suitable for the planting mat of AVI in an oligotrophic lake where nutrients are limited for growth of hydrophytes.

• 한국미생물·생명공학회지
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• 제47권3호
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• pp.412-422
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• 2019

The present study reports the morphological and molecular characterization of the fungal strain, CMSS06 and evaluates its raw starch hydrolyzing ability in four different agricultural substrates (rice bran, banana peel, cassava tubers, and coconut water). The potential use of each agricultural substrate to replace the expensive fermentation media was evaluated with six different fermentation media: rice bran (RB), banana peel (BP), cassava starch (CS), cassava in coconut water (CSCW), cassava in modified coconut water (CMCW), and pure Coconut water (CW). The fungal strain CMSS06 was identified as Thielaviopsis ethacetica by the analysis of the ITS sequences. The T. ethacetica alpha amylase enzyme exhibited maximum alpha amylase activity at 72 h, pH 7.0, and $40^{\circ}C$ on soluble starch. This species resulted in the highest enzyme activity (mU/ml) of 26.06, 10.89, 58.82, 14.2, and 54.67 with the RB, BP, CS, CSCW, and CMCW fermentation media, respectively. The results indicate that CS can be used as a carbon substrate and CMCW can be used to accelerate the fermentation by T. ethacetica. The enzyme was partially purified by 40-60% ammonium sulphate fraction, and it showed total enzyme activity, total protein content, specific activity, purification fold, and a recovery of 2400 mU, 30 mg, 80 mU/mg, 2.7, and 71.1%, respectively. The molecular mass of the T. ethacetica alpha amylase was estimated on SDS-PAGE, and two bands around 50 kDa and 70 kDa were identified. The present study implies that T. ethacetica can produce alpha amylase, and it can be used to hydrolyze raw starch during the fermentation processes.

• 한국자원식물학회지
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• 제35권4호
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• pp.508-514
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• 2022

Bulbophyllum auricomum Lindl. is very popular among orchid growers due to the attractive fragrance of its flowers and has become an endangered orchid in Myanmar. In this study, we carried out an aseptic technique that can be used to conserve this endangered orchid species. The seeds of B. auricomum Lindl. were obtained from artificial pollination and cultured in MS basal medium for seed germination. The effect of coconut water and BAP in MS basal medium on callus induction was investigated. The highest callus induction was found at 2.0 mg/L BAP. The maximum growth of protocorm-like bodies (PLBs) was evaluated, and the best response was observed on MS medium supplemented with 150 mL/L coconut water at pH 5.6. MS basal medium supplemented with 150 mL/L coconut water along with 2.0 mg/L BAP and 1.0 mg/L NAA (MCBN) showed the highest number of plantlets at 15℃ at the second week of culture. At the second and third week of culture, MS medium supplemented with 2.0 mg/L BAP and 1.0 mg/L NAA (MBN) showed the best result in terms of the number of leaves and the longest leaves at 15℃ and 25℃, respectively. The present study showed evidence of successful in vitro propagation of B. auricomum Lindl.

• 대한환경공학회지
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• 제31권9호
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• pp.747-752
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• 2009

본 연구에서는 다양한 재질의 입상활성탄들을 이용하여 연속흡착실험에서 $BrO_3\;^-$의 파과특성을 조사하였다. 활성탄 재질별 파과시점은 석탄계 재질의 활성탄들이 가장 늦게 파과에 도달하였으며, 다음으로 야자계와 목탄계 재질의 활성탄으 로 나타났다. 활성탄 g당 $BrO_3\;^-$에 대한 최대 흡착량(X/M)은 석탄계 재질의 활성탄들이 각각 1334.5 ${\mu}g$/g 및 798.2 ${\mu}g$/g으로 가장 컸으며, 야자계 668.6 ${\mu}g$/g, 목탄계 156.8 ${\mu}g$/g으로 나타났고, 석탄계 활성탄들이 야자계와 목탄계에 비하여 최대 흡착량이 약 2.0배~8.5배 정도 큰 것으로 조사되었다. $BrO_3\;^-$에 대한 CUR은 석탄계 재질의 활성탄들이 각각 0.19 g/day과 0.25 g/day, 야자계와 목탄계가 각각 0.33 g/day과 0.71 g/day로 나타났다. 활성탄의 흡착용량을 나타내는 k값의 경우 석탄계 재질의 활성탄들이 각각 121.3과 76.7로 나타나 야자계와 목탄계의 43.3과 14.6에 비하여 석탄계 활성탄들이 월등히 높은 k값을 나타내어 다른 재질의 활성탄에 비하여 $BrO_3\;^-$ 흡착용량이 큰 것으로 조사되었다.

• 공업화학
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• 제30권5호
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• pp.530-535
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• 2019

본 연구에서는 coconut oil과 sugar ester를 이용한 유화과정에 대한 최적화공정을 수행하였다. 최적화를 위해 반응표면분석법 중 중심합성계획모델을 이용하였다. 반응표면분석법의 반응치로는 유화액의 점도, 평균입자크기와 7 days 경과 후 ESI 등을 설정하였으며, 계량인자로는 유화시간, 유화속도 및 유화제의 첨가량을 설정하였다. 중심합성계획모델로 최적화과정을 수행한 결과 계량인자는 유화시간(22.63 min), 유화속도(6,627.41 rpm) 및 유화제의 첨가량(2.29 wt.%)에서 최적실험값인 점도(1,707.56 cP), 평균입자크기(1,877.05 nm) 및 7 days 경과 후 ESI (93.23%)로 종합만족도(D = 0.8848)가 높게 나타났다. 또한 이 조건에서 실제 실험을 통해 얻은 결과는 이론결과와 비해 평균오차율 $1.2{\pm}0.1%$로 작게 나타났다. 따라서 coconut oil의 유화과정에 반응표면분석법 중 중심합성계획법을 적용할 경우 매우 낮은 오차율을 얻을 수 있었다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권1호
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• pp.31-37
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• 2002

This paper was investigated to clarify the possibility of a biodegradation of materials adsorbed on different porous granular-activated carbons (GACs) such as coal-& coconut-based GAC. Total organic carbon, humic substance and ammonia were used to compare their removal efficiencies. The objective of this study is to determine the adsorption capacity of bioregenerated GAC. When raw water reacted with chloride, the yield of THMs increased as a function of the input amount of chloride. The formation of trihalomethanes (THMs) was investigated in water treated with chlorine when humic acid was used as THM precursor. As the input amount of chloride in raw water increased by two or five-fold to remove the $NH_3$, the chloroform of the THMs significantly increased also five or ten-fold. It was found that the chloroform was significantly removed by the treatment of biological activated carbon (BAG) in comparison with the ozone treatment, and the removal efficiency of THMs in coal-typed GAC was $10-30\%$ better than coconut-typed GAC due to the biological degradation on the surface of the activated carbons.

• 농업생명과학연구
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• 제44권5호
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• pp.9-13
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• 2010

본 연구는 절화용 국화의 양액재배를 위한 코코넛 배지의 이용 가능성을 검토하기 위해 수행되었다. 본 연구에서 코코넛 배지는 중과피에서 유래된 dust와 fiber 그리고 가공된 chip을 이용하여 조성하였다. 시험구의 처리는 코코넛 단용배지 (dust 100%), dust와 fiber 그리고 dust와 chip을 각각 70:30의 부피비로 혼합한 혼용배지, 그리고 비교를 위해 펄라이트를 대조구로 하였다. 배지별 함수율은 펄라이트배지에서 75.8%로 가장 낮았고 코코넛 더스트 단용배지에서 93.1%로 가장 높게 나타났다. 배액의 pH는 코코넛 단용 및 혼용배지에서 5.8 ~ 6.5사이였으나 펄라이트배지에서 6.7 ~ 7.3의 범위로 높게 나타났다. 배액의 EC는 코코넛 단용배지에서 가장 높고 펄라이트배지에서 가장 낮은 것으로 나타났다. 국화의 초장, 엽면적, 그리고 건물량은 펄라이트배지와 코코넛 단용배지와 비교하여 코코넛 혼용배지에서 우수한 것으로 나타났다. 배지처리별 개화소요일수는 차이가 없었다.

• 셀메드
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• 제6권2호
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• pp.11.1-11.7
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• 2016

The aimed of the presence study was to determine the antiulcer potential of virgin coconut oil (VCO), either extracted by wet process (VCO_A) or fermentation process (VCO_B), and to compare their effectiveness against the copra oil (CO) using the HCl/ethanol-induced gastric ulcer model. Earlier, the oils underwent chemical analysis to determine the free fatty acids composition, physicochemical properties and anti-oxidant capability. In the antiulcer study, rats (n=6) were pre-treated orally for 7 consecutive days with distilled water (vehicle), 100 mg/kg ranitidine (positive group) or the respective oils (10, 50, and 100% concentration). One hour after the last test solutions administration on Day 7^th, the animals were subjected to the gastric ulcer assay. Macroscopic and microscopic analyses were performed on the collected rat's stomachs. From the results obtained, the chemical analysis revealed i) the presence of high content of lauric acid followed by myristic acid and palmitic acid in all oils and; ii) the significant (^*p< 0.05) different in anisidine- and peroxide-value, percentage of free fatty acid, total phenolic content and total antioxidant activity among the oils. The animal study demonstrated that all oil possess significant (^*p< 0.05) antiulcer activity with VCO_B being the most effective oil followed by VCO_A and CO. The macroscopic observations were supported by the microscopic findings. Interestingly, all oils were more effective than 100 mg/kg ranitidine (reference drug). In conclusion, coconut oils exert remarkable antiulcer activity depending on their methods of extraction, possibly via the modulation of its antioxidant and anti-inflammatory activity.

• Journal of Plant Biotechnology
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• 제7권4호
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• pp.247-257
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• 2005

An efficient, rapid and large-scale in vitro clonal propagation of agronomically important Indian cereal crop genotypes (NSH27 & K5) of Sorghum bicolor (L.) Moench. by enhanced shoot proliferation in shoot tip segments was designed. MS medium fortified with plant growth regulators and coconut water markedly influenced in vitro propagation of Sorghum bicolor. In vitro plantlet production system has been investigated on Murashige and Skoog (MS) medium with the synergistic combination of 6-benzyladenine ($22.2\;{\mu}M$), kinetin ($4.6\;{\mu}M$), adenine sulphate ($2.8\;{\mu}M$), 5% coconut water and 3% sucrose which promoted the maximum number of shoots as well as beneficial shoot length. Subculturing of shoot tip segments on a similar medium enabled continuous production of more than 100 healthy shoots with similar frequency. When the healthy shoot clumps were cultured on MS medium fortified with 6-benzyladenine ($22.2\;{\mu}M$), kinetin ($4.6\;{\mu}M$), adenine sulphate ($2.8\;{\mu}M$), ${\alpha}$-naphthaleneacetic acid ($2.7\;{\mu}M$), ascorbic acid ($30.0\;{\mu}M$) and 5% coconut water, a rapid production of axillary and adventitious buds was developed after 8 wk culture. More than 300 shoots were produced 10 wk after culture. Rooting was highest (100%) on half strength MS medium containing 22.8 mM IAA. Micropropagated plants established in garden soil, farmyard soil and sand (2:1:1) were uniform and identical to the donor plant with respect to growth characteristics. These plants grew normally without showing any traits.

• Journal of Plant Biotechnology
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• 제43권1호
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• pp.138-145
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• 2016

To find the optimal propagation condition for endangered Cypripedium macranthos Sw., also known as lady's slipper orchid, the effect of various organic additives on in vitro germination, protocorm formation and seedling growth was investigated during asymbiotic seed culture. When $100ml{\cdot}L^{-1}$ coconut water was added to the basal medium, the highest germination rate and protocorm formation rate were achieved, with 70.8% and 74.2% respectively. Supplementation of phloem sap from birch tree or maple tree also showed a facilitating effect to improve the germination and protocorm development. With $100ml{\cdot}L^{-1}$ birch sap or maple sap, both the germination and protocorm formation rates were roughly more than 65% and 68%. The roots and buds of the seedlings grew vigorously in the medium containing $100ml{\cdot}L^{-1}$ coconut water or phloem sap, in particular, their bud formation rates increased by more than 70%. Addition of banana powder and peptone could not create a more significantly favorable culture condition, and non-addition had the worst results. Our results demonstrated that proper organic amendments such as coconut water and phloem sap might be preferred to in vitro germination and the growth of seedlings developed from the protocorm of C. macranthos Sw. during asymbiotic seed culture.